The antioxidant activity of androgen therapy is the basis for preventing complications in men with the type 2 diabetes mellitus

Abstract Objective: Uric acid is an end product of purine metabolism and it has two different functions such as pro-oxidant and anti-oxidant. Where, pro-oxidant and anti-oxidants are opposite in action. Oxidative stress and endothelial dysfunction are a foremost cause of complications in diabetes mellitus, where uric acid may play a major role in this process. Hence, the present study has been designed to evaluate antioxidant activity of uric acid and its correlation with oxidative stress and endothelial dysfunction in type 2 diabetic subjects. Methods: We included 120 subjects in this study with age group of 39 -60 years. Among these 60 were type 2 diabetic subjects and 60 were healthy controls. The estimation of biochemical parameters such as blood sugar, lipid profile, uric acid, and homocysteine are measured in fully auto-analyzer with well recognized methods. MDA measured by TBARS method, total antioxidant capacity as FRAP and NO estimated by Kinetic cadmium method in spectrophotometer. Results: The study was found significant elevation of triglyceride, LDL and MDA and significant lower level of FRAP and NO in T2DM than healthy control. Uric acid was insignificant in T2DM compared to healthy control. However, uric acid has significant correlation with FRAP (r=0.2116, p=0.02) and moderate correlation with triglyceride (r=0.1736, p=0.0579) and homocysteine (r=0.1779, p=0.0519). MDA was negatively and NO was positively correlated with uric acid but statistically insignificant. Conclusion: We have found antioxidant activity of uric acid where it was determined by significant positive correlation with FRAP in type 2 diabetes mellitus.

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Food-derived cyanidin-3-O-glucoside alleviates oxidative stress: Evidence from islet cell line and diabetic db/db mice

Food & Function ◽

10.1039/d1fo02385c ◽

2021 ◽

Author(s):

Xiang Ye ◽

Wen Chen ◽

Pengcheng Tu ◽

Ruoyi Jia ◽

Yangyang Liu ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Antioxidant Activity ◽

Type 2 Diabetes Mellitus ◽

Health Outcomes ◽

Cell Line ◽

Islet Cell ◽

Adverse Health Outcomes

Type 2 diabetes mellitus is a disease associated with oxidative milieu that often leads to adverse health outcomes. Multiple anthocyanins have been reported to possess outstanding antioxidant activity, however, their...

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Biosynthesis of Copper Nanoparticles using Mucuna Pruriens and its Antioxidant and Antidiabetic Activity

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i47b33163 ◽

2021 ◽

pp. 621-629

Author(s):

Shifa Jawahar Ali ◽

R. V. Geetha ◽

S. Rajeshkumar

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Antioxidant Activity ◽

Type 2 Diabetes Mellitus ◽

Side Effects ◽

Copper Nanoparticles ◽

Mucuna Pruriens ◽

Antidiabetic Activity ◽

Antidiabetic Agent

Introduction: The field of Nanotechnology has gained importance since last century. Nanoparticles can be used in medicine due to its increased interaction with microbes and has less side effects than drugs. Antioxidant compounds scavenge free radicals and inhibit the oxidative mechanisms that lead to degenerative diseases. There is a growing number of diabetes patients all over the world. Wide varieties of synthetic drugs are being used for the treatment of Type 2 diabetes mellitus, most of them possess side effects in the long run such as hepatotoxicity, abdominal pain, flatulence and diarrhea. Therefore, there is a need for a search of an alternate antidiabetic agent Aim: The aim of the study is to synthesize Copper nanoparticles from Mucuna pruriens and to evaluate its antioxidant and antidiabetic activity. Materials and methods: Plant extract of Mucuna pruriens was prepared and filtered by Whatman No 1 filter paper. Copper sulphate was added to the plant extract and kept in a magnetic stirrer for nanoparticle synthesis. The synthesized nanoparticle was preliminarily analysed using UV visible spectroscopy. Finally the left over solution was taken to calculate antioxidant activity and antidiabetic activity. Results: Antioxidant activity was calculated by DPPH method and the percentage of inhibition of copper nanoparticles synthesised from Mucuna pruriens was 58.5% for 10µL, 59.6% for 20µL, 67.5% for 30µL, 71.4% for 40µL and 72.3% for 50µL. Antidiabetic activity was calculated by alpha-amylase inhibitory assay and the percentage of inhibition of copper nanoparticles synthesised from Mucuna pruriens was 66% for 10µL, 69% for 20µL, 73% for 30µL, 79% for 40µL and 80% for 50µL. Conclusion: We can conclude that copper nanoparticles synthesised from Mucuna pruriens are a potent antioxidant and antidiabetic agent. Since it shows a good activity in free radical scavenging, copper nanoparticles can be used in a clinical therapeutic application and also in the management of type 2 diabetes mellitus.

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Evaluation of the Inhibition of Carbohydrate Hydrolyzing Enzymes, the Antioxidant Activity, and the Polyphenolic Content ofCitrus limettaPeel Extract

The Scientific World JOURNAL ◽

10.1155/2014/121760 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 18

Author(s):

Eduardo Padilla-Camberos ◽

Estefania Lazcano-Díaz ◽

José Miguel Flores-Fernandez ◽

Moses S. Owolabi ◽

Kirk Allen ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Antioxidant Activity ◽

Type 2 Diabetes Mellitus ◽

Antioxidant Activities ◽

Chronic Hyperglycemia ◽

Hydrolyzing Enzymes ◽

Dose Dependent ◽

Citrus Limetta

Type 2 diabetes mellitus is one of the most frequent causes of death in Mexico, characterized by chronic hyperglycemia. One alternative strategy for this metabolic abnormality is inhibiting the enzymes responsible for the metabolism of carbohydrates. We evaluated whether the aqueousCitrus limettapeel extract could inhibit the metabolism of carbohydrates. We found that this extract inhibited primarily the enzymeα-amylase by 49.6% at a concentration of 20 mg/mL and to a lesser extent the enzymeα-glucosidase with an inhibition of 28.2% at the same concentration. This inhibition is likely due to the high polyphenol content in theCitrus limettapeel (19.1 mg GAE/g). Antioxidant activity of theCitrus limettapeel demonstrated dose-dependent antioxidant activity, varying from 6.5% at 1.125 mg/mL to 42.5% at 20 mg/mL. The study of these polyphenolic compounds having both antihyperglycemic and antioxidant activities may provide a new approach to the management of type 2 diabetes mellitus.

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