Antioxidant Activity of Uric Acid and its Correlation with Oxidative Stress and Endothelial Dysfunction in Type 2 Diabetes Mellitus

2020 ◽  
Author(s):  
P. Siva Prasad ◽  
P. Hari Prasad ◽  
T. Sudhakar ◽  
B. Rajkumar ◽  
Adithya Raj ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Antioxidant Activity ◽  
Type 2 Diabetes Mellitus ◽  
Uric Acid ◽  
Endothelial Dysfunction ◽  
Healthy Control ◽  
Type 2 Diabetic

Abstract Objective: Uric acid is an end product of purine metabolism and it has two different functions such as pro-oxidant and anti-oxidant. Where, pro-oxidant and anti-oxidants are opposite in action. Oxidative stress and endothelial dysfunction are a foremost cause of complications in diabetes mellitus, where uric acid may play a major role in this process. Hence, the present study has been designed to evaluate antioxidant activity of uric acid and its correlation with oxidative stress and endothelial dysfunction in type 2 diabetic subjects. Methods: We included 120 subjects in this study with age group of 39 -60 years. Among these 60 were type 2 diabetic subjects and 60 were healthy controls. The estimation of biochemical parameters such as blood sugar, lipid profile, uric acid, and homocysteine are measured in fully auto-analyzer with well recognized methods. MDA measured by TBARS method, total antioxidant capacity as FRAP and NO estimated by Kinetic cadmium method in spectrophotometer. Results: The study was found significant elevation of triglyceride, LDL and MDA and significant lower level of FRAP and NO in T2DM than healthy control. Uric acid was insignificant in T2DM compared to healthy control. However, uric acid has significant correlation with FRAP (r=0.2116, p=0.02) and moderate correlation with triglyceride (r=0.1736, p=0.0579) and homocysteine (r=0.1779, p=0.0519). MDA was negatively and NO was positively correlated with uric acid but statistically insignificant. Conclusion: We have found antioxidant activity of uric acid where it was determined by significant positive correlation with FRAP in type 2 diabetes mellitus.

scholarly journals Correlation of Uric Acid with Oxidative Stress and Endothelial Dysfunction in Type 2 Diabetes Mellitus

2021 ◽  
Author(s):  
Siva Prasad Palem
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Uric Acid ◽  
Endothelial Dysfunction ◽  
P Value ◽  
Healthy Controls ◽  
Antioxidant Power

Objective: To evaluate the correlation of uric acid with oxidative stress and endothelial dysfunction in type 2 diabetic subjects.Material and Methods: The study included 120 subjects, among when 60 were type 2 diabetes subjects and the remaining 60 were age and gender matched healthy controls. The biochemical parameters, blood glucose, lipid profile, uric acid and homocysteine, were measured by standard kits in an autoanalyzer. Oxidative stress was evaluated by measuring malondialdehyde (MDA) and total antioxidant power by manual methods such as thio-barbituric acid reactive substances and ferric reducing ability of plasma (FRAP). Endothelial dysfunction was assessed by measuring nitric oxide (NO) by the kinetic cadmium method.Results: A significant elevation of triglycerides, low density lipoprotein (LDL), and MDA were observed in the type 2 diabetes mellitus patients while FRAP and NO were significantly reduced compared to the healthy controls. In addition, the uric acid levels had a highly significant correlation with FRAP (r=0.212, p-value=0.020), and moderately significant correlation with triglycerides (r=0.173, p-value=0.057) and homocysteine (r=0.178, p-value=0.051). Uric acid was negatively correlated with MDA and positively correlated with NO, but not statistically significant.Conclusion: Our findings suggest that uric acid may have antioxidant properties since it had a significant positive correlation with FRAP.

scholarly journals GTP Cyclohydrolase I Gene Polymorphisms Are Associated with Endothelial Dysfunction and Oxidative Stress in Patients with Type 2 Diabetes Mellitus

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e108587 ◽  
Author(s):  
Pawel P. Wolkow ◽  
Wladyslaw Kosiniak-Kamysz ◽  
Grzegorz Osmenda ◽  
Grzegorz Wilk ◽  
Beata Bujak-Gizycka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Endothelial Dysfunction ◽  
Gene Polymorphisms ◽  
Gtp Cyclohydrolase I ◽  
And Oxidative Stress ◽  
I Gene

scholarly journals Histone deacetylase 3 inhibition alleviates type 2 diabetes mellitus-induced endothelial dysfunction via Nrf2

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Shuai Huang ◽  
Gen Chen ◽  
Jia Sun ◽  
Yunjie Chen ◽  
Nan Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Endothelial Dysfunction ◽  
Histone Deacetylase ◽  
Vascular Endothelium ◽  
Ros Production ◽  
Histone Deacetylase 3

Abstract Background The mechanism underlying endothelial dysfunction leading to cardiovascular disease in type 2 diabetes mellitus (T2DM) remains unclear. Here, we show that inhibition of histone deacetylase 3 (HDAC3) reduced inflammation and oxidative stress by regulating nuclear factor-E2-related factor 2 (Nrf2), which mediates the expression of anti-inflammatory- and pro-survival-related genes in the vascular endothelium, thereby improving endothelial function. Methods Nrf2 knockout (Nrf2 KO) C57BL/6 background mice, diabetic db/db mice, and control db/m mice were used to investigate the relationship between HDAC3 and Nrf2 in the endothelium in vivo. Human umbilical vein endothelial cells (HUVECs) cultured under high glucose-palmitic acid (HG-PA) conditions were used to explore the role of Kelch-like ECH-associated protein 1 (Keap1) –Nrf2–NAPDH oxidase 4 (Nox4) redox signaling in the vascular endothelium in vitro. Activity assays, immunofluorescence, western blotting, qRT-PCR, and immunoprecipitation assays were used to examine the effect of HDAC3 inhibition on inflammation, reactive oxygen species (ROS) production, and endothelial impairment, as well as the activity of Nrf2-related molecules. Results HDAC3 activity, but not its expression, was increased in db/db mice. This resulted in de-endothelialization and increased oxidative stress and pro-inflammatory marker expression in cells treated with the HDAC3 inhibitor RGFP966, which activated Nrf2 signaling. HDAC3 silencing decreased ROS production, inflammation, and damage-associated tube formation in HG-PA-treated HUVECs. The underlying mechanism involved the Keap1–Nrf2–Nox4 signaling pathway. Conclusion The results of this study suggest the potential of HDAC3 as a therapeutic target for the treatment of endothelial dysfunction in T2DM.

scholarly journals HbA1c is a risk factor for cardiovascular disease in association with oxidative stress in patients with type 2 diabetes mellitus

2017 ◽  
Vol 5 (7) ◽  
pp. 3114 ◽  
Author(s):  
Siva Prasad Palem
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Cardiovascular Disease ◽  
Type 2 Diabetes Mellitus ◽  
Risk Factor ◽  
Healthy Controls ◽  
High Level ◽  
Type 2 Diabetic

Background: Cardiovascular disease (CVD) is a major cause of death in diabetic subjects. Glycaemic status is one of the vital factor involved in vascular complications. It was clear the effect of glycaemia on microvascular complications, but uncertain on macrovascular complications. As we know oxidative stress plays a major role in the development of atherosclerosis and cardiovascular disease. Since oxidative stress is a risk factor for cardiovascular disease, the study has designed to perceive an association between HbA1c and oxidative stress in patients with type 2 diabetes mellitus for early prediction of cardiovascular events.Methods: 120 subjects were taken into the study, among these 60 type 2 diabetic subjects and remaining 60 subjects were healthy controls. The parameters like HbA1c, MDA and FRAP were estimated by established methods. ‘Kruskal Wallis’ test was used for variables in the parameters and Pearson correlation test was used to perform correlation between HbA1c and oxidative stress.Results: High level of HbA1c and MDA, low level of FRAP were found in patients with type 2 diabetes than healthy controls. The study was also found HbA1c have positive association with malondialdehyde (MDA) and negative association with FRAP.Conclusions: HbA1c was positively associated with oxidative stress in patients with type 2 diabetes mellitus. In this scenario, type 2 diabetic patients with high level of HbA1c might have risk of cardiovascular events.

scholarly journals Age-dependent systemic DNA damage in early Type 2 Diabetes mellitus

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
Dinko Rogulj ◽  
Ismail El Aklouk ◽  
Konjevoda Paško ◽  
Spomenka Ljubić ◽  
Mirjana Pibernik Okanović ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dna Damage ◽  
Type 2 Diabetes Mellitus ◽  
Uric Acid ◽  
Oxidative Dna Damage ◽  
Ldl Cholesterol ◽  
Age Dependent

Oxidative stress, capable of eliciting damage to various biomolecules including DNA, is a recognized component of diabetes mellitus and its complications. Metabolic syndrome  (MetS) is associated with the development of type 2 diabetes mellitus  (T2DM), as well as other unfavorable outcomes. The aim of this study was to elucidate the role of oxidative stress in the development of T2DM, by investigating association of oxidative DNA damage with metabolic parameters in subjects with MetS and early T2DM.Selected anthropometric and biochemical parameters of MetS, inflammation and oxidative DNA damage: body mass index  (BMI), fatty liver index  (FLI), waist circumference  (WC), total cholesterol, HDL  and LDL-cholesterol, GGT, uric acid, total leukocyte/neutrophil count,  and urinary 8-oxo-deoxyguanosine  (u-8-oxodG) were assessed in male subjects with MetS and both younger  (≤55 years) and older  (>55 years) subjects with T2DM of short duration without complications.BMI, FLI, WC, total and LDL-cholesterol and  uric acid were higher, while the u-8-oxodG was lower in MetS group, when compared to older T2DM subjects. None of these parameters were different neither between MetS and younger T2DM, nor between two sub-groups of subjects with T2DM. Values of CRP, HDL-cholesterol, triglycerides, GGT, leukocytes and neutrophils were not different between all examined groups of subjects. Age-dependent increase in u-8-oxodG suggests that aging process, rather than metabolic disturbances or diabetes per se plays a pivotal role in development of oxidative DNA-damage in T2DM. Oxidative DNA damage cannot serve as an universal early marker of T2DM.

scholarly journals Effect of oxidative stress on endothelium in patients with heart failure and type 2 diabetes ­mellitus

2020 ◽  
Vol 101 (1) ◽  
pp. 13-17
Author(s):  
F I Mammadova
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Diabetes Mellitus ◽  
Heart Failure ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Chronic Heart Failure ◽  
Endothelial Dysfunction ◽  
Control Group

Aim. To estimate the severity of endothelial dysfunction and effects of nitric oxide, thiol status and cystatin on the progression of chronic heart failure and chronic heart failure in type 2 diabetes mellitus. Methods. 80 patients (men and women) with chronic heart failure were included. All patients were divided into two groups: the first group 39 patients with chronic heart failure, the second 41 people with chronic heart failure and type 2 diabetes mellitus. The control group consists of 20 healthy donors. To obtain statistically significant differences with the control group the minimum sample size for observations was determined based on the target variance of a small sample (n=10). The lipid profile and carbohydrate metabolism, endothelin-1, cystatin, nitric oxide were evaluated. Statistical processing was performed using Microsoft Office Excel and IBM SPSS Statistics 20 software. Results. Changes in lipid metabolism were found in both groups, while an increase in carbohydrate metabolism was observed in patients with chronic heart failure with type 2 diabetes mellitus. Under conditions of oxidative stress in patients with chronic heart failure, a decrease in the content of thiol status and an increase in the amount of nitric oxide in the blood serum were recorded. The endothelin-1 level was elevated, particularly in the second group, which indicates a more serious endothelial dysfunction with increased glucose content in patients with chronic heart failure. Conclusion. The level of cystatin C as an atherogenic risk factor was equally increased in the studied patients, possibly it affected by the rate of disease progression; feasible to use these markers to detect the progression of chronic heart failure in the early stages.

scholarly journals Relationship between FVC, FEV1, FEV1/ FVC% and oxidative stress in type 2 diabetes mellitus

2020 ◽  
Vol 14 (2) ◽  
pp. 69-76
Author(s):  
Shomia Farid Tanni ◽  
Sultana Ferdousi ◽  
Md Saiful Islam
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diabetic Patients ◽  
Pulmonary Impairment ◽  
The Mean ◽  
Type 2 Diabetic ◽  
Restrictive Pattern

Background: Diabetes mellitus causes micro and macrovascular disorder with debilitating effects on many organs including lungs. There is pulmonary impairment in type 2 diabetic patients (T2DM) which is usually characterized by restrictive pattern. Increased oxidative stress is associated with type 2 diabetes which may contribute to microvascular and macrovascular complications. Objectives:To assess the relationship between oxidative stress and lung function in patients with type 2 diabetes mellitus. Methods: This cross sectional study was carried out in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka from September’2018 to August’ 2019. For this study, 35 newly diagnosed type 2 diabetic male patients aged 30-50 years and similar age and BMI 35 apparently healthy subjects were enrolled as control. Forced vital capacity (FVC), Forced expiratory volume in 1st second (FEV1) and FEV1/FVC%were assessed by portable spirometer. For evaluation of oxidative stress, plasma malondialdehyde (MDA) and catalase levels were measured by competitive ELISA technique and spectrophotometry. Statistical analysis was done by unpaired ‘t’ test, chi-square test, pearson correlation test and multiple regression analysis as applicable. Results: In this study, the mean percentage of predicted value of FVC and FEV1were significantly lower (p<0.001) in T2DM. In addition restrictive pattern of pulmonary function was found in 65.71% and 14.28% in T2DM and healthy control respectively and the difference was statistically significant. The mean plasma catalase was significantly lower (p<0.01) and plasma MDA was significantly higher (p<0.001) in patients. In addition, FVC showed significant negative correlation and significant association with higher MDA level in T2DM. Moreover, FEV1 also showed significant association with MDA in T2DM. Conclusion: The present study reveals that restrictive pattern of pulmonary impairment is related to oxidative stress in T2DM. J Bangladesh Soc Physiol. 2019, December; 14(2): 69-76

scholarly journals CIRCULATING SERUM ADIPONECTIN AND OXIDATIVE STRESS BIOMARKERS IN PREDIABETES AND TYPE 2 DIABETES MELLITUS PATIENTS

2019 ◽  
pp. 58-60
Author(s):  
RACHNA SHARMA ◽  
SATYANARAYANA P ◽  
PALLAVI ANAND ◽  
SHRAWAN KUMAR
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Uric Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diabetic Patients ◽  
Oxidative Stress Biomarkers ◽  
And Oxidative Stress

Objectives: Adipocytokines and oxidative stress have been independently studied in various morbidities, but their interrelationship in mediating insulin resistance and type 2 diabetes mellitus is still unclear. Thus, the present study was aimed at evaluating malondialdehyde (MDA) levels in pre-diabetes and diabetics and correlating it with adiponectin levels. Methods: Fifty controls, 50 pre-diabetic, and 50 diabetic patients were enrolled. Plasma MDA, uric acid, and adiponectin were measured in the blood samples. Serum MDA adiponectin was analyzed by enzyme-linked immunosorbent assay and uric acid was analyzed by uricase method. Results: Pre-diabetic and diabetic patients had higher levels of MDA and uric acid, while the level of adiponectin was low compared to controls. Correlation of MDA was positive with uric acid but negative with adiponectin in diabetic patients. Likewise, adiponectin and uric acid were also correlated negatively. In pre-diabetes, adiponectin was significantly and negatively correlated with MDA and uric acid. Conclusions: Pre-diabetic and diabetic patients have increased oxidative stress, which is also linked with adipokine abnormalities. From this study, we observed that oxidative stress suppresses adiponectin production which is the protective adipokine in hyperglycemia. Thus, oxidative stress may serve as an indicator or target for in the control of hyperglycemic stress.

Food-derived cyanidin-3-O-glucoside alleviates oxidative stress: Evidence from islet cell line and diabetic db/db mice

2021 ◽  
Author(s):  
Xiang Ye ◽  
Wen Chen ◽  
Pengcheng Tu ◽  
Ruoyi Jia ◽  
Yangyang Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Antioxidant Activity ◽  
Type 2 Diabetes Mellitus ◽  
Health Outcomes ◽  
Cell Line ◽  
Islet Cell ◽  
Adverse Health Outcomes

Type 2 diabetes mellitus is a disease associated with oxidative milieu that often leads to adverse health outcomes. Multiple anthocyanins have been reported to possess outstanding antioxidant activity, however, their...

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