scholarly journals ISOLATION OF ALGAL VIRUSES OF TETRASELMIS VIRIDIS AND PHAEODACTYLUM TRICORNUTUM FROM BOTTOM SEDIMENTS OFF THE COAST OF CRIMEA AND SOUTH-WEST RUSSIA

2019 ◽  
Vol 4 ◽  
pp. 123-127
Author(s):  
Author(s):  
O.A. Stepanova ◽  

Virus monitoring is a widespread control of their background circulation. Since 2002, the monitoring of algal viruses that infect microalgae Tetraselmis viridis and Phaeodactylum tricornutum has been carried out in the bays of Sevastopol differing in ecological conditions. The purpose of this work is to analyze and assess the results of this long-term (2002–2020) monitoring. The results obtained with taking into account the exactingness or resistance to environmental conditions of hosts of algal viruses that infect microalgae T. viridis and P. tricornutum were the basis for the following conclusions regarding the ecological well-being of the studied bays of Sevastopol: – relatively favorable ecological conditions in 2007–2008, 2017–2018 and in 2020. (maximum frequency of isolation PtV, and/or minimum TvV, and / or maximum difference in frequency of isolation between PtV and TvV); – unfavorable ecological conditions in 2002–2003, 2006 and 2015 (maximum frequency of isolation TvV, and/or minimum PtV, and/or maximum difference in frequency of isolation between TvV and PtV). Analysis of the monitoring results of algal viruses of T. viridis and P. tricornutum in 2015–2020 revealed that the studied relatively ecologically safe bays are characterized by the maximum number of all isolates of algal viruses (both TvV and PtV) and the numerical predominance of strains of algal virus of P. tricornutum, which is demanding to environmental conditions. At the same time, the minimum amount of all viral isolates was recorded in the ecologically unfavorable bay. The results of a long-term monitoring of algal viruses of T. viridis and P. tricornutum have established the possibility of using the monitoring of marine viruses, in particular of algal viruses of microalgae which are indicative of the ecological situation, as a component of complex ecological monitoring as ecological indicator.


Author(s):  
O.A. Stepanova ◽  
◽  
P.V. Gaisky ◽  
S.A. Sholar ◽  
◽  
...  

The change in sensitivity to viral infection of cultures of three species of Black Sea microalgae (Tetraselmis viridis, Dunaliella viridis and Phaeodactylum tricornutum) after exposure to a constant unidirectional magnetic field with a magnetic induction of 600 G is experimentally studied. The studies were carried out with using a developed laboratory installation. With the duration of the experiments 24 h and 48 h, it was recorded that being in a magnetic field resulted in an increase in the resistance to viral lysis in microalgae T. viridis, its decrease in P. tricornutum and no changes in D. viridis. We assume that the revealed changes in the sensitivity of microalgae to viral infection after exposure to a magnetic field are due to their species characteristics and selectivity.


Author(s):  
O.A. Stepanova ◽  
◽  
P.V. Gaisky ◽  
S.A. Sholar ◽  
◽  
...  

In three experiments aimed at studying the effect of a constant magnetic field with a magnetic induction of 600 G and a duration of experiments of 24 and 36 h, it was found that with a low initial titer of the algal virus of the microalgae Tetraselmis viridis, its stay in a magnetic field for 24 h led to an increase in the infectious titer by one order of magnitude. However, when the virus with a higher infectious titer was in a magnetic field for 24 h, no changes in the titer were observed, and after 36 h, its decrease by one order of magnitude was recorded. The results obtained indicate the need for further research in this direction, with using a wider range of algal viruses.


The described fauna has been extracted from the sediments of the Nechells channel of Hoxnian Interglacial age, whose stratigraphy and flora have already been discussed. It comprises a small number of species of fish, ostracods, Cladocera, mites, molluscs and polyzoa and a considerable number of insects with beetles predominant. The distribution of these has been related to the floral zones of Kelly (1964). The earliest sediments are those of a glacially dammed lake and the limited fauna associated with these are ostracods, Daphnia, Cristatella and, amongst the insects, the larvae of Sialis , trichoptera and chironomids. Shortly after, fish and molluscs appear and by Zone IN 3, beetles first become significant. After IIN a , beetles form the predominant element of the fauna. Calcareous and phosphatic organisms disappear after IIN a , corresponding to a change in the nature of the bottom sediments, but this is largely explained by post-depositional solution. The time and climate distribution of Daphnia and Cristatella mucedo , both of which have winter resting stages, is discussed in relation to climate, as are beetles of the genus Rhynchaenus in relation to the occurrence of food plants, and the chironomids in relation to temperature and limnology. The coleopterous fauna, the first of considerable size to be described from a period of the Pleistocene as old as the Hoxnian, is still essentially British and the identity with present-day species is striking. An exceptional case of this identity is provided by the aedeagus dissected from Hydraena riparia . Only three non-British species have been named and two of these, Platypus oxyurus and Brachytemnus submricatus , from near the top of the sequence, have now a disjunct occurrence in southern Europe. Micropeplus caelatus has been identified and this now has a sporadic distribution in central Europe and is restricted in Britain to the extreme south-west of Ireland. There are no beetles characteristic of cold climate but the earliest fauna as a whole clearly indicates glacial conditions. The climate ameliorates to reach its optimum, a little warmer but not much different from that of today, towards the end of the borehole sequence, and the deposits do not include a fauna characteristic of the second (cooling) half of an interglacial.


2019 ◽  
Vol 25 ◽  
pp. 317
Author(s):  
Ayotunde Ale ◽  
Opeyemi Aloro ◽  
Ayanbola Adepoju
Keyword(s):  

2019 ◽  
Vol 25 ◽  
pp. 121-122
Author(s):  
Olufunmilayo Adeleye ◽  
Ejiofor Ugwu ◽  
Anthonia Ogbera ◽  
Akinola Dada ◽  
Ibrahim Gezawa ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document