Wx gene polymorphism in winter triticale collection samples

2020 ◽  
pp. 80-87
Author(s):  
O. Levchenko
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Starch Content ◽  
Infrared Spectrometry ◽  
Wild Type ◽  
Chain Reaction ◽  
Winter Triticale ◽  
Allelic State ◽  
Wx Gene ◽  
Polymerase Chain

The purpose of the study was to identify the collection of winter triticale in the allelic state of the waxi-genes and to identify sources with the presence of waxi-alleles for these genes. The surveys were conducted over 2017–2019 at the NSc Institute of Agriculture. The subject of the research are 43 collection samples of winter triticale, 29 of which are numbers of own breeding, 14 – breeding varieties of the National Institute of Agriculture of NAAS (9) and scientifi c institutions of Poland (1) and the Russian Federation (4). For control, we used soft winter waxy-wheat Sofi yka and wheat with wild of starch Oksana. Field, laboratory (infrared spectrometry, light microscopy, polymerase chain reaction (PCR)) methods, weights and mathematical and statistical methods of research were used to evaluate the collection material. According to the results of molecular genetic analysis of the Wx gene polymorphism in the winter triticale collection samples, it was found that all the tested samples had wild type alleles according to the Wx-B1 gene and were characterized by the absence of the Wx-D1 gene. The Wx-A1 gene revealed samples with both wild-type alleles and presence in the genome of the wax-allele. 8 collections with Wx-A1 gene alleles were selected: selection numbers 141, 153, 201, 223, 229 and varieties Lubomir, Petrol and Poliskii 7. The selected samples varied signifi cantly in terms of such characteristics as grain productivity, weight of 1000 grains, starch content. The tendency to decrease the size of the granules and increase the evenness of the granulometric structure of the starch in the samples with the presence of the wax-allele of the Wx-A1 gene was established. Wx-A1 gene allele samples are valuable starting material for the creation of new winter triticale varieties with increased amylopectin starch suitable for bioethanol processing. Key words: winter triticale, bioethanol, starch, polymerase chain reaction, amylopectin, amylose, allelic state of wax genes, waxi-allele, wild type.

scholarly journals Polymorphisms of CYP1A1 Genes and Its Correlation with Clinical Variant of Pterygium

2020 ◽  
Vol 1 (2) ◽  
pp. 116-123
Author(s):  
Hendriati ◽  
Vitresia H
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Dna Isolation ◽  
Wild Type ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Cyp1a1 Gene ◽  
Clinical Variant ◽  
Polymerase Chain ◽  
Mutant Homozygote

  Background and Objective: CYP1A1 gene, which has role in carcinogenic metabolisms, is also detected in pterygium tissue. The aim of the study is to determine the polymorphisms of CYP1A1 m2 (rs1048943) and m4 (rs1799814) gene and its correlation with clinical variant of the pterygium. Methods: DNA isolation was performed from blood sample of 80 pterygium patients consisting of 40 inflammatory and 40 non-inflammatory pterygium. Genotyping of rs1048943 SNP AG (m2) in the CYP1A1 gene was performed using Alel Specific Polymerase Chain reaction (AS-PCR) and rs1048943) SNP Genotyping was performed using PCR. Polymorphism results are characterized as wild type (AA), mutant homozygote (GG), and mutant heterozygote (AG). Results: CYP1A1 m2 and m4 gene polymorphism consist of wild type (AA), mutant homozygote (GG), and mutant heterozygote (AG). Both CYP1A1 m2 and m4 genes polymorphism of both groups of inflammatory and non-inflammatory pterygium was mostly consist of wild type polymorphism, followed by the mutant heterozygote polymorphism. The wild type polymorphism was found to be higher in inflammatory pterygium, meanwhile the mutant heterozygote was found to be higher in non-inflammatory pterygium. Conclusion: There were differences in CYP1A1 m2 and m4 gene polymorphism in both pterygium group, but none has been shown to be statistically associated with the clinical variant of the pterygium.

Low frequency of CYP2A6 gene polymorphism as revealed by a one-step polymerase chain reaction method

1999 ◽  
Vol 9 (3) ◽  
pp. 327-332 ◽  
Author(s):  
Gen-Fu Chen ◽  
Yong-Ming Tang ◽  
Bridgett Green ◽  
Dong-Xin Lin ◽  
F. Peter Guengerich ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Low Frequency ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
One Step ◽  
Polymerase Chain ◽  
Cyp2a6 Gene ◽  
Step Polymerase Chain Reaction

Polymorphisms of LHβ and GnRHR genes and their association with the number of embryos recovered in goats

2014 ◽  
Vol 54 (8) ◽  
pp. 987 ◽  
Author(s):  
M. Z. Fu ◽  
G. Li ◽  
Z. Q. Zhou
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Nucleotide Polymorphisms ◽  
Gene Polymorphism ◽  
Corpus Luteum ◽  
Nucleotide Polymorphisms ◽  
Chain Reaction ◽  
Single Strand Conformational Polymorphism ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Polymerase Chain

The objective of the present study was to explore a predictor of superovulation response on the basis of associations between the number of embryos recovered and gene polymorphism. Variation in the goat LHβ and GnRHR genes was investigated using polymerase chain reaction–single-strand conformational polymorphism and DNA sequencing. Two single nucleotide polymorphisms (SNPs) were identified in the 5′-UTR of LHβ gene (A59C, P1 locus) and in the Exon 2 of GnRHR gene (T177A, P6 locus). At the P1 locus in both breeds, the frequencies of one allele were 0.46 and 0.51, respectively. At the P6 locus, the minor allele frequency was 0.23. Associations of both SNPs with the number of embryos recovered and the corpus luteum number were evaluated in Boer and Shaanbei goat breeds. Association analysis showed that both SNPs had significant (P < 0.05) effects on the number of embryos recovered and corpus luteum number. These results indicate that LHβ and GnRHR genes are potential markers for the number of embryos recovered.

Population pharmacokinetic analysis of S-1 including the CYP2A6 genotype in patients with advanced cancer

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 2507-2507
Author(s):  
T. Hirose ◽  
K. Nishimura ◽  
K. Fujita ◽  
M. Adachi ◽  
Y. Sasaki ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Advanced Cancer ◽  
Variant Allele ◽  
Population Pharmacokinetic ◽  
Wild Type ◽  
Chain Reaction ◽  
History Of ◽  
Polymerase Chain ◽  
Variant Alleles ◽  
The Individual

2507 Background: S-1 is an oral anticancer agent composed of tegafur, CDHP, and potassium oxonate. Tegafur is a prodrug of fluorouracil (5-FU), and CDHP prevents degradation of 5-FU by inhibiting dihydropyrimidine dehydrogenase and enhances the anticancer activity of 5-FU. The biotransformation of tegafur to 5-FU is demonstrated to be catalyzed by CYP2A6. CYP2A6 polymorphisms are seen more frequently in Japanese people than Caucasian. Therefore, we performed a population pharmacokinetic (PPK) analysis of S-1 including the CYP2A6 genotype in Japanese patients with advanced cancer and developed a model describing the disposition kinetics of tegafur, CDHP, and 5-FU after oral administration of S-1. Methods: Fifty-eight patients with advanced cancer were eligible if they had a performance status of 0 to 3 and had adequate organ function. A dose of 80 mg/m2 of S-1 was given orally twice daily for 28 consecutive days, followed by 14 days of rest. The PPK analysis was performed with plasma concentration data for tegafur, CDHP, and 5-FU. The CYP2A6 genotype was analyzed with the polymerase chain reaction-restriction fragment length polymorphism method or an allele-specific polymerase chain reaction-based method. On the basis of the CYP2A6 genotype, all patients were classified into 1 of 3 groups: wild type, 1 variant allele, and 2 variant alleles. Results: Creatinine clearance correlated with the individual clearance of CDHP. Body surface area correlated with the individual clearance and volumes of CDHP and tegafur. In patients with 2 variant alleles of CYP2A6, tegafur clearance was 58% less than that in patients with wild type or 1 variant allele of CYP2A6. In addition, in patients with a history of gastrectomy, the absorption rate constant of tegafur was 66% higher than that in patients with no history of gastrectomy. The time-varying concentration of CDHP was the most appropriate model component describing the inhibitory effect on 5-FU catabolism. The individual Bayesian predictions of CDHP, tegafur, and 5-FU concentrations based on the present PPK model were in good agreement with the observed data. Conclusions: This is the first PPK model of S-1 including the CYP2A6 genotype. No significant financial relationships to disclose.

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