SIMULTANEOUS ESTIMATION OF PARACETAMOL AND ETODOLAC BY HPLC METHOD IN PURE AND COMBINATION FORMULATION

A simple, accurate and precise stability-indicating RP-HPLC method was developed and subsequently validated for simultaneous determination of ilaprazole and domperidone in bulk and pharmaceutical dosage form. The proposed HPLC method utilizes C18 (250 mm × 4.6 mm × 5 µm) column with mobile phase comprising of 0.5 % glacial acetic acid in water pH 5.5 adjusted with ammonia solution: methanol in the ratio 45:55 v/v at a flow rate of 1.0 ml/min. Quantitation was achieved with UV detection at 286 nm based on peak area with linear calibration curves at concentration ranges 80-120µg/ml for ilaprazole and 240-360 µg/ml for domperidone with correlation coefficient of 0.999.The retention times of ilaprazole and domperidone were found to be 3.0 min, 5.4 min respectively. The mean recoveries obtained for ilaprazole and domperidone were found to be 99.76 ± 0.6463 % and 100.7 ± 0.2424 % respectively. Stress testing which covered acid, alkali hydrolysis, and peroxide, photolytic, thermal degradation was performed to prove the specificity of the proposed method and degradation was achieved. The proposed method was successfully applied for the stability indicating simultaneous estimation of ilaprazole and domperidone in routine quality control analysis in bulk and pharmaceutical formulation.

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Novel Validated RP-HPLC Method for Simultaneous Estimation of Valsartan & Gliclazide in Bulk and Dosage Forms

Current Pharmaceutical Analysis ◽

10.2174/1573412913666170613090348 ◽

2018 ◽

Vol 14 (4) ◽

pp. 412-418

Author(s):

Rasmita Patra ◽

Prakash Narayana Reddy ◽

N.S. Sampath Kumar ◽

Vijaya R. Dirisala

Keyword(s):

Dosage Forms ◽

Hplc Method ◽

Simultaneous Estimation ◽

Rp Hplc

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Development and validation of RP-HPLC method for simultaneous estimation of teneliglipt in hydro bromide hydrate and glimepiride in bulk and tablet dosage form

International Journal of Pharma and Bio Sciences ◽

10.22376/ijpbs.2019.10.2.p18-25 ◽

2019 ◽

Vol 10 (2) ◽

Author(s):

I.PONN ILAVARASAN ◽

TAMILSELVI N ◽

ABINAYA N ◽

GAYATHRI R

Keyword(s):

Dosage Form ◽

Hplc Method ◽

Simultaneous Estimation ◽

Rp Hplc ◽

Development And Validation ◽

Tablet Dosage

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Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Codeine Phosphate and Chlorpheniramine Maleate from Their Combined Liquid Dosage Form

Chromatography Research International ◽

10.1155/2013/404727 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Ramakrishna Kommana ◽

Praveen Basappa

Keyword(s):

Limit Of Detection ◽

Degradation Products ◽

Hplc Method ◽

Simultaneous Estimation ◽

Forced Degradation ◽

Codeine Phosphate ◽

Chlorpheniramine Maleate ◽

Combination Drug ◽

Stability Indicating ◽

Rp Hplc

The present paper describes the development of quick stability indicating RP-HPLC method for the simultaneous estimation of codeine phosphate and chlorpheniramine maleate in the presence of its degradation products, generated from forced degradation studies. The developed method separates codeine phosphate and chlorpheniramine maleate in impurities/degradation products. Codeine phosphate and chlorpheniramine maleate and their combination drug product were exposed to acid, base, oxidation, dry heat, and photolytic stress conditions, and the stressed samples were analysed by proposed method. The proposed HPLC method utilizes the Shimadzu HPLC system on a Phenomenex C18 column (, 5 μ) using a mixture of 1% o-phosphoric acid in water : acetonitrile : methanol (78 : 10 : 12) mobile phase with pH adjusted to 3.0 in an isocratic elution mode at a flow rate of 1 mL/min, at 23°C with a load of 20 μL. The detection was carried out at 254 nm. The retention time of codeine phosphate and chlorpheniramine maleate was found to be around 3.47 min and 9.45 min, respectively. The method has been validated with respect to linearity, robustness, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ). The developed validated stability indicating HPLC method was found to be simple, accurate, and reproducible for the determination of instability of these drugs in bulk and commercial products.

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A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations

E-Journal of Chemistry ◽

10.1155/2011/607069 ◽

2011 ◽

Vol 8 (s1) ◽

pp. S41-S46

Author(s):

Prafulla Kumar Sahu ◽

M. Mathrusri Annapurna ◽

Dillipkumar Sahoo

Keyword(s):

High Performance ◽

Internal Standard ◽

Pharmaceutical Formulations ◽

High Performance Liquid Chromatographic ◽

Hplc Method ◽

Simultaneous Estimation ◽

Aqueous Acetic Acid ◽

Linear Calibration ◽

Acceptance Range ◽

Liquid Chromatographic

This paper describes a high-performance liquid chromatographic method for simultaneous estimation of nabumetone and paracetamol in binary mixture. The method was based on RP-HPLC separation and quantitation of the two drugs on hypersil C-18 column (250 mm × 4.6 mm) using a mobile phase consisting of acetonitrile and 0.05% aqueous acetic acid (70:30v/v) at flow rate of 1 mL min-1. Quantitation was achieved with PDA detector at 238 nm based on peak area with linear calibration curves at concentration ranges 5-25 µg mL-1for both the drugs. Naproxen sodium was used as internal standard. The method has been successively applied to pharmaceutical formulation. No chromatographic interference from the tablet excipients was found. The method was validated in terms of precision, robustness, recovery and limits of detection and quantitation. The intra and inter-day precision and accuracy values were in the acceptance range as per ICH guidelines.

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