DEVELOPING PLANT REGENERATION SYSTEMS FOR IN VITRO CONSERVATION OF MANDARIN (CITRUS RETICULATA) AND PUMMELO (CITRUS MAXIMA)

2005 ◽  
pp. 133-136 ◽  
Author(s):  
R.A. Avenido ◽  
L.E. Endonela ◽  
L.F. Pateña ◽  
R.C. Barba
Keyword(s):  
Plant Regeneration ◽  
In Vitro Conservation ◽  
Citrus Reticulata ◽  
Citrus Maxima ◽  
Regeneration Systems

Effects of low temperature on Chrysanthemum shiwogiku var. kinokuniense in vitro conservation

2018 ◽  
Vol 44 (4) ◽  
pp. 675-678
Author(s):  
Weilan ◽  
Shucheng Xu ◽  
Xuelan Zhu
Keyword(s):  
Plant Regeneration ◽  
Low Temperature ◽  
Genetic Stability ◽  
Test Tube ◽  
In Vitro Conservation ◽  
Young Plant ◽  
In Vitro Plantlets ◽  
Surviving Rate ◽  
Issr Pcr

The effect of low temperature condition on wild Chrysanthemum shiwogiku var. in vitro conservation was investigated, and its plant regeneration hereditary stability was detected using its sterile seedling. The results show that the test tube young plant grows rapidly, and its preservation time is short at a temperature at 25±2°C, at condition of 12 h/d, photoperiod at 2000 ~ 3000 lx, and all die after 180 days. However, the sterile seedling grows slowly under low-temperature, and its preservation time extends to 360 days. The sterile seedling surviving rate was above 96%. The data indicate that the 4°C low temperature is advantageous to Chrysanthemum plantlets preservation. After preservation, the recovered plantlets grow well and show no differences in morphology and isoenzyme zymogram of peroxidase, ISSR-PCR compared with the control. In addition, the results show that low temperature in vitro plantlets maintain genetic stability.

scholarly journals Establishment of a Plant Regeneration and Genetic Transformation System of Panax ginseng C.A. Meyer

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 572d-572
Author(s):  
Hak-Tae Lim ◽  
Haeng-Soon Lee ◽  
Tage Eriksson
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Genetic Transformation ◽  
Adventitious Shoots ◽  
Shoot Multiplication ◽  
Regeneration System ◽  
Transformation Protocol ◽  
Genetic Transformation System ◽  
Regeneration Systems

Plant regeneration of ginseng has been known to be difficult, and there are a few reports on plant regeneration of ginseng via somatic embryogenesis. In vitro flowering has, however, been one of the major drawbacks in these regeneration systems in which BA and GA3 were included in germination and shoot multiplication media. Multiplication of adventitious shoots from a single somatic embryo, abnormal morphology, and vitrified shoots were also observed. All these facts have made successful acclimatization of ginseng plantlets difficult. The purposes of this study were 1) to establish the plant regeneration system via organogenesis, 2) to improve normal plant regeneration via somatic embryogenesis, 3) to improve the efficiency of plant regeneration from protoplast culture, 4) to understand the acclimatization process, 5) to develop effective genetic transformation protocol. Data in relation with all these studies are presented in detail.

scholarly journals Respon Pertumbuhan Kultur In Vitro Jeruk Besar (Citrus maxima (Burm.) Merr.) cv. Nambangan terhadap Osmotikum dan Retardan

2015 ◽  
Vol 5 (1) ◽  
pp. 21
Author(s):  
Iswari S. Dewi ◽  
Gani S. Jawak ◽  
Bambang S. Purwoko ◽  
M. Sabda
Keyword(s):  
Active Compound ◽  
Growth Response ◽  
In Vitro Conservation ◽  
Green Leaf ◽  
In Vitro Growth ◽  
Minimal Growth ◽  
Plant Materials ◽  
Inhibition Of Growth ◽  
Citrus Maxima

<p>ABSTRACT</p><p>In vitro  conservation has been applied to many species. However, the suppression of explant growth is essential for extending the duration of conservation.  The objective of the research was  to study  in  vitro  growth response  of  pummelo  cv.  Nambangan  to  conservation  medium containing osmotically  active  compound  (osmoticum)  or  growth  suppressant (retardant).  Two  sets  of experiments were conducted  using randomized complete design and replicated three times.  In vitro shoot with four leaves from pummelo, namely cultivar Nambangan, were used as the plant materials. The treatment in the first experiment was  MS + osmoticum (mannitol  0, 20, 40,  and 60 g L-1) and  in the  second  experiment  was  MS +  retardant  (paclobutrazol  0,  1,  3  and  5  mg  L-1).  The  resultsindicated that senescence of the leaf was induced by 20, 40, and 60 g L-1of mannitol. The best media in inhibition of growth  for  pummelo cv. Nambangan was MS + paclobutrazol  1  mg  L-1.  With this media, plant was inhibited but grew normally with green leaf and root.</p><p>Keywords: mannitol, minimal growth, paclobutrazol, pummelo</p><p> </p><p>ABSTRAK</p><p>Konservasi  in  vitro   sudah  banyak  dilakukan  pada  berbagai  spesies. Penghambatan  pertumbuhansangat  penting  bagi  lamanya  tanaman  dapat disimpan.  Tujuan  penelitian  ini  adalah  mempelajari respon  pertumbuhan  in vitro  pamelo  cv.  Nambangan  terhadap  media  konservasi mengandung osmotikum  atau  penghambat  pertumbuhan  (retardan).  Dua percobaan  dilakukan  terpisah  menggunakan  rancangan  acak  lengkap  dan diulang  3  kali.  Tunas  hasil  perbanyakan  in  vitro  dengan  4 daun, digunakan  sebagai  eksplan.  Perlakuan  pada  percobaan  pertama  adalah  MS +  osmotikum (mannitol 0, 20, 40, 60 g L-1) dan pada percobaan kedua adalah MS + retardan (paclobutrazol 0, 1, 3,5 mg L-1). Hasil menunjukkan bahwa daun mengalami senesen oleh perlakuan mannitol. Media yang direkomendasikan untuk konservasi pamelo cv. Nambangan  adalah  MS + paclobutrazol 1 mg  L-1. Dengan media tersebut pertumbuhan dihambat, tetapi tetap normal, berakar dengan daun tetap hijau.</p><p>Kata kunci: mannitol, pertumbuhan minimal, paclobutrazol, pamelo</p>

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

2015 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kee Hwa Bae ◽  
Eui Soo Yoon
Keyword(s):  
Plant Regeneration ◽  
Seed Germination ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Ornamental Plants ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Temperature Treatment ◽  
In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

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