scholarly journals In vitro variation in antibacterial activity plant extracts on Glaucium elegans and saffron (Crocus sativus)

2014 ◽  
Vol 9 (3) ◽  
Author(s):  
Ehsan Heidari Soureshjani ◽  
Mina Heidari
1970 ◽  
Vol 1 (4) ◽  
pp. 82-88 ◽  
Author(s):  
MJ Foysal ◽  
MM Rahman ◽  
M Alam

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88


Antibiotics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 89 ◽  
Author(s):  
Shahabe Abullais Saquib ◽  
Nabeeh Abdullah AlQahtani ◽  
Irfan Ahmad ◽  
Mohammed Abdul Kader ◽  
Sami Saeed Al Shahrani ◽  
...  

Background: In the past few decades focus of research has been toward herbal medicines because of growing bacterial resistance and side effects of antimicrobial agents. The extract derived from the plants may increase the efficacy of antibiotics when used in combination against pathogenic bacteria. In the current study, the synergistic antibacterial efficacy of plant extracts in combination with antibiotics has been assessed on selected periodontal pathogens. Methods: Ethanolic extracts were prepared from Salvadora persica (Miswak) and Cinnamomum zeylanicum (Ceylon cinnamon), by the soxhalate method. Plaque samples were collected from clinical periodontitis patients to isolate and grow the periodontal pathobionts under favorable conditions. Susceptibility of bacteria to the extracts was assessed by gauging the diameter of the inhibition zones. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts were determined against each bacterium. Synergistic activity of plants extract in combination with antibiotics against the bacteria was also assessed by measuring the diameter of the inhibition zones. Results: Ethanolic extract of both the plants showed an inhibitory effect on the proliferation and growth of all four strains of periodontal pathobionts. Maximum antibacterial activity was exhibited by C. zeylanicum against Tannerella forsythia (MIC = 1.56 ± 0.24 mg/mL, MBC = 6.25 ± 0.68 mg/mL), whereas among all the studied groups the minimum activity was reported by C. zeylanicum against Aggregatibacter actinomycetemcomitans the (MIC = 12.5 ± 3.25 mg/mL, MBC = 75 ± 8.23 mg/mL). Combination of herbal extracts with different antibiotics revealed a synergistic antibacterial effect. The best synergism was exhibited by S. persica with metronidazole against A. actinomycetemcomitans (27 ± 1.78). Conclusions: Current in vitro study showed variable antibacterial activity by experimented herbal extracts against periodontal pathobionts. The synergistic test showed significant antibacterial activity when plant extracts were combined with antibiotics.


Horticulturae ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 36
Author(s):  
Kamal A. M. Abo-Elyousr ◽  
Najeeb M. Almasoudi ◽  
Ahmed W. M. Abdelmagid ◽  
Sergio R. Roberto ◽  
Khamis Youssef

The aim of this study is to assess the effect of extracts of Nerium oleander, Eucalyptus chamadulonsis and Citrullus colocynthis against bacterial spot disease of tomato and to investigate the induction of resistance by tomato (Solanum lycopersicum) in order to promote a sustainable management system. The antibacterial activity of aqueous and ethanol plant extracts was tested against Xanthomonas axonopodis pv. vesicatoria, isolate PHYXV3, in vitro and in vivo. The highest antibacterial activity in vitro was obtained with C. colocynthis, N. oleander and E. chamadulonsis, respectively. In vivo, ethanol extracts of N. oleander and E. chamadulonsis were more effective than aqueous extracts in reducing pathogen populations on tomato leaves. Under greenhouse conditions, application of the plant extracts at 15% (v/v) to tomato plants significantly reduced disease severity and increased the shoot weight of ‘Super Marmande’ tomato. In most cases, plant extracts significantly increased total phenol and salicylic acid content of tomato plants compared to either healthy or infected ones. In addition, C. colocynthis and E. chamadulonsis extracts significantly increased peroxidase activity while only E. chamadulonsis increased polyphenol oxidase after infection with the causal agent. The results indicated that the plant extracts showed promising antibacterial activity and could be considered an effective tool in integrated management programs for a sustainable system of tomato bacterial spot control.


2018 ◽  
Vol 19 (1) ◽  
pp. 35
Author(s):  
Esti Handayani Hardi ◽  
Gina Saptiani ◽  
Nurkadina Nurkadina ◽  
Irawan Wijaya Kusuma ◽  
Wiwin Suwinarti

The aim of this study was to evaluate the antibacterial activity of ethanol concoction (mixed extract) of three herbs plants, such as: Boesenbergia pandurata, Solanum ferox and Zingimber zerumbet against Aeromonas hydrophila, Pseudomonas sp. and both bacteria. The Plant extracts were obtained by using ethanol and the concentration of the extract was 600 mgL-1 of B. pandurata, 900 mgL-1 of S. ferox and 200 mgL-1 of Z. zerumbet. The inhibition zone was measured after 6, 12, 18, 24 h incubation at 30o C. The ratios of concoction S. ferox and B. pandurata (for 100 mL solution) were 90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90. The same ratios were made on the concoction of S. ferox and Z. zerumbet. The concoctions of S. ferox and B. pandurata in the ratio of 50:50 and 60:40, and the concoction of S. ferox and Z. Zerumbet with ratio 60:40 had higher antibacterial activity against A. hydrophila single isolate compared to the other concoctions. Furthermore, the concoction extract of S. ferox and B. pandurate 50:50 and the combined of S. ferox and Z. zerumbet ratio 50:50 and 90:10 were the best combination to inhibit the growth of a single bacterium Pseudomonas sp. The combined S. ferox and B. pandurata ratio 50:50; 10:90 and S. ferox mixed with Z. zerumbet ratio 50:50 and 40:60 were the best combination against the combined bacteria between A. hydrophila and Pseudomonas sp. The conclusion of this research was the combined extract of S. ferox and B. pandurata and S. ferox with Z. zerumbet are effective to suppress the growth of single or combination of A. hydropila and Pseudomonas sp.


2015 ◽  
Vol 9 (14) ◽  
pp. 1020-1025
Author(s):  
A C Duailibe Silvana ◽  
R Macedo Cost Maria ◽  
Lima Kenio ◽  
Uzeda Milton

2011 ◽  
Vol 25 (11) ◽  
pp. 1059-1066 ◽  
Author(s):  
M. Hajimahmoodi ◽  
M. Shams-Ardakani ◽  
P. Saniee ◽  
F. Siavoshi ◽  
M. Mehrabani ◽  
...  

2021 ◽  
Vol 13 (Aquaculture) ◽  
pp. 106-112
Author(s):  
Thi My Duyen Tran ◽  
Trong Tuan Nguyen ◽  
Thi Tuyet Hoa Tran

Crude methanol extract of 9 Vietnamese plants were in vitro screened for their antibacterial activity against three common freshwater fish pathogens including Aeromonas hydrophila, Edwardsiella ictaluri, and Streptococcus agalactiae. Agar disc diffusion method was used to evaluate the antibacterial activity, then solvent extract was performed for the extracts which exhibited the strongest and a broad-spectrum antibacterial activity. Minimal inhibitory concentration (MIC) was conducted for effective plant extracts using broth dilution method. The results indicated that most of the plant extracts exhibited antibacterial propeties to at least one tested bacterium. Headache tree (Premna corymbosa), bushwillows (Combretum quadrangulare) and Celandine spider flower (Cleome chelidonii) showed a broad-spectrum antibacterial activity. The largest inhibitory zones of 35 mm and 21 mm were observed  for the extract of Premna corymbosa against E. ictaluri and S. agalactiae, respectively. E. ictaluri was found to be the most susceptible for all of the extracts while A. hydrophila was the most resistant. The MIC of effective plant extracts against tested bacteria ranged between 0.39 mg/mL and 3.125 mg/mL. The result can be considered for further investigation of the development of an alternative therapy against bacterial infection in aquaculture.


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