GENETIC DIVERGENCE IN DOUBLED HAPLOID LINES OF SPRING WHEAT (Triticum aestivum L.)

Genetic divergence of 17 doubled haploid lines and 3 check varieties of spring wheat were assessed through Mahalanobis D2-statistics. The genotypes were grouped into six clusters. The clusters I and III contained the highest number of lines (5). The cluster V contained single genotype. The maximum inter-cluster distance was noticed between the clusters I and V and minimum between III and VI. The highest intra-cluster distance was observed in the cluster IV and lowest in the cluster V.DOI: http://dx.doi.org/10.3329/bjpbg.v20i2.17034

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Gametoclonal variation detected in the nuclear ribosomal DNA from doubled haploid lines of a spring wheat (Triticum aestivum L., cv. ?C�sar?)

Theoretical and Applied Genetics ◽

10.1007/bf00290079 ◽

1987 ◽

Vol 74 (1) ◽

pp. 31-37 ◽

Cited By ~ 30

Author(s):

A. Rode ◽

C. Hartmann ◽

A. Benslimane ◽

E. Picard ◽

F. Quetier

Keyword(s):

Triticum Aestivum ◽

Ribosomal Dna ◽

Spring Wheat ◽

Doubled Haploid ◽

Triticum Aestivum L ◽

Nuclear Ribosomal Dna ◽

Doubled Haploid Lines ◽

Gametoclonal Variation

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Cytogenetic studies of progenies from crosses between 'Centurk' wheat and its doubled haploids derived from anther culture

Genome ◽

10.1139/g89-490 ◽

1989 ◽

Vol 32 (4) ◽

pp. 622-628 ◽

Cited By ~ 8

Author(s):

Sawsan S. Youssef ◽

R. Morris ◽

P. S. Baenziger ◽

C. M. Papa

Keyword(s):

Triticum Aestivum ◽

Chromosome Pairing ◽

Doubled Haploid ◽

Structural Changes ◽

Meiotic Chromosome ◽

Doubled Haploids ◽

Triticum Aestivum L ◽

Root Tips ◽

Doubled Haploid Lines ◽

A Minor

Karyotype stability, which is essential when using wheat (Triticum aestivum L.) doubled haploids in a breeding program, was evaluated in 14 anther-derived doubled-haploid lines after at least three generations of selfing, by crossing them as females with the parent cultivar 'Centurk' and doing cytological studies on the progenies. There were no deviations from the hexaploid chromosome number (2n = 42) in root tips. Meiotic chromosome pairing was as stable as that in the control ('Centurk' × 'Centurk') in most progenies. Chromosomal structural changes and (or) behavioral deviations were detected at the metaphase I, anaphase I, telophase I, and quartet stages of meiosis in a minor proportion of the cells. The frequencies of multivalents, lagging bivalents and univalents, bridges, and micronuclei were higher in some progenies than in the control. Chromosomal fragments were infrequent. The ranges in percentages of normal cells were 72.4–90.0 at anaphase I, 76.4–92.6 at telophase I, and 82.6–93.2 at quartet stages in the doubled-haploid progenies, compared with 95–100, 92–100, and 94–96, respectively, in the control. On the basis of these results, the doubled-haploid lines should produce enough normal gametes to provide adequate seed supplies when they are used as parents in wheat cultivar and population improvement.Key words: Triticum aestivum, chromosome pairing, chromosome aberrations, gametoclonal variation.

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Stettler hard red spring wheat

Canadian Journal of Plant Science ◽

10.4141/cjps08227 ◽

2009 ◽

Vol 89 (5) ◽

pp. 945-951 ◽

Cited By ~ 25

Author(s):

R M DePauw ◽

R E Knox ◽

F R Clarke ◽

J M Clarke ◽

T N McCaig

Keyword(s):

Triticum Aestivum ◽

Grain Yield ◽

Spring Wheat ◽

Doubled Haploid ◽

Triticum Aestivum L ◽

Kernel Weight ◽

Hard Red Spring Wheat ◽

Volume Weight ◽

Grain Volume Weight ◽

End Use

Based on 34 replicated trials over 3 yr, Stettler, a doubled haploid hard red spring wheat (Triticum aestivum L.), expressed significantly higher grain yield than all checks except Superb. Wheat and flour protein concentration were significantly greater than all of the checks except Lillian. It matured significantly later than AC Barrie and Katepwa but earlier than Superb. Stettler was significantly shorter than all of the checks except Superb and was more resistant to lodging than Katepwa and Laura. Stettler had high grain volume weight and intermediate kernel weight relative to the checks, and meets the end-use quality specifications of the Canada Western Red Spring wheat market class. Stettler expressed resistance to prevalent races of stem rust, common bunt and loose smut, with moderate susceptibility to prevalent races of leaf rust and fusarium head blight.Key words: Triticum aestivum L., cultivar description, grain yield, protein, disease resistance, doubled haploid

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GENETIC DIVERSITY FOR HEAT TOLERANCE TRAITS IN SPRING WHEAT (Triticum aestivum L.)

Bangladesh Journal of Plant Breeding and Genetics ◽

10.3329/bjpbg.v25i2.18663 ◽

2012 ◽

Vol 25 (2) ◽

pp. 15-23

Author(s):

A. A. Khan ◽

M. Mahbubur Rahman ◽

M. F. Amin ◽

M. K. Alam ◽

M. A. Alam

Keyword(s):

Genetic Diversity ◽

Heat Tolerance ◽

Spring Wheat ◽

Genetic Divergence ◽

Flag Leaf ◽

Grain Filling ◽

Triticum Aestivum L ◽

Cluster Distance ◽

Heat Tolerant ◽

Late Sowing

The present investigation was undertaken with the objective to identify the extent of genetic diversity for traits related to heat tolerance among 25 currently available spring wheat genotypes under late sowing condition during the cropping season 2009-2010. All genotypes were distributed into five clusters showing considerable genetic divergence for most of the heat tolerant traits under study. The role of grain filling rate and biomass production in both the vectors had the highest contribution to genetic divergence. The positive values of both the vectors for flag leaf senescence, ground coverage, spikes number, 1000-grain weight, grain yield (g m-2) and harvest index indicating high contribution of these traits towards the divergence among 25 genotypes of wheat. Three pair of clusters, viz. I & II, I & III and I & V can be considered for obtaining more heterotic progeny as the genetic distance between these clusters were larger. Considering yield performances, cluster distance and cluster mean the genotype G-22 from cluster I and genotype G-14 from cluster IV may be considered better parents for further breeding of heat tolerance as they showed maximum divergence and high degree of tolerance to heat under late sowing condition. Moreover, the genotype G-22 could be exploited for direct release as a heat tolerant variety after testing under wider range of environments.DOI: http://dx.doi.org/10.3329/bjpbg.v25i2.18663

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Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture

Czech Journal of Genetics and Plant Breeding ◽

10.17221/113/2019-cjgpb ◽

2020 ◽

Vol 56 (No. 4) ◽

pp. 150-158

Author(s):

Osama Zuhair Kanbar ◽

Csaba Lantos ◽

Paul Karumba Chege ◽

Erzsébet Kiss ◽

János Pauk

Keyword(s):

Triticum Aestivum ◽

Winter Wheat ◽

Anther Culture ◽

Doubled Haploid ◽

Genetic Material ◽

Triticum Aestivum L ◽

Average Value ◽

Doubled Haploid Lines ◽

Dh Lines

We investigated the anther culture (AC) efficiency of thirteen F<sub>4</sub> combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.

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DIVERGENCE ANALYSIS BASED ON YIELD AND YIELD CONTRIBUTING TRAITS OF A COLLECTION OF SPRING WHEAT GENOTYPES (Triticum aestivum L.)

Bangladesh Journal of Plant Breeding and Genetics ◽

10.3329/bjpbg.v28i1.27846 ◽

2015 ◽

Vol 28 (1) ◽

pp. 17-22

Author(s):

M. S. Rahman ◽

L. Ali ◽

A. Sultana ◽

M. Ruhullah ◽

M. S. Hossain

Keyword(s):

Genetic Diversity ◽

Triticum Aestivum ◽

Spring Wheat ◽

Broad Spectrum ◽

Triticum Aestivum L ◽

Principal Coordinate Analysis ◽

Grain Weight ◽

Wheat Genotypes ◽

Cluster Distance ◽

1000 Grain Weight

Genetic diversity among 24 spring wheat genotypes (Varieties/lines) was estimated using Mahalanobis D2-statistics and principal coordinate analysis. The genotypes fell into four clusters of different size. Of the seven different characters spike length and 1000 grain weight had the highest contribution towards the divergence. The highest inter cluster distance was observed between cluster I and III followed by cluster II and III. So, genotypes from the most divergence clusters could be used as parents in hybridization program and are expected to manifest maximum heterosis as well as broad spectrum of variability.

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