scholarly journals Human Papilloma Virus and Other Risk Factors of Carcinoma Cervix

1970 ◽  
Vol 38 (1) ◽  
pp. 18-23 ◽  
Author(s):  
S Khatun ◽  
Syed Md Akram Hussain ◽  
F Hossain ◽  
A Choudhury
Keyword(s):  
Risk Factors ◽  
Cervical Cancer ◽  
Cancer Screening ◽  
Human Papilloma Virus ◽  
Sexual Activity ◽  
Hpv Infection ◽  
Carcinoma Cervix ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Screening Strategies

Cervical cancer is the most common cancer among women in many developing countries constituting 20%-30% of female cancers. The etiological factors of cervical cancer include environmental, social, sexual and sexually transmitted agents including human papilloma virus (HPV). HPV has identified in 99.7% invasive cancer. Objective of the study was to find out the prevalence of HPV-DNA virus among diagnosed cases of preinvasive and invasive diseases and to find out different sociodemographic parameters related to carcinoma cervix. This was a cross sectional study. The study has conducted in the department of Obstetrics and Gynaecology of Bangabandhu Sheikh Mujib Medical University. Duration of study was from May 2005 to May 2006. Samples were collected from diagnosed cases of CIN I, II, III and CIS and invasive cancer. Thirty cases were recruited for collection of cervical scraping. Sample were collected of in a specially designed sampler containing liquid transport medium and preserved in minus 20 Degree Celsius and sent to the DNA laboratory for processing and identification of HPV by hybrid capture II method. The results were analyzed by using SPSS version 11.0 and shown in tables and diagrams. The risk factors for HPV infection identified from the result. These are early sexual activity, years of sexual activity, urban people, and higher number of pregnancies. The test could identify HPV-DNA in 100 % of invasive and 50 % of preinvasive cancer. In this study HPV infection was found as a strong determinant for the development of cervical carcinoma and its precursors. Poor socio-economic condition, lower level of education, early marriage and first delivery, multi-parity, years of sexual activity are the risk factors for developing HPV infection. New guidelines for cervical cancer screening strategies could be able to develop from the information obtained from this study. A population-based study with larger sample will be required for the evaluation of cervical cancer screening strategies. Key words: HPV, Carcinoma Cervix   DOI: 10.3329/bmj.v38i1.3582 Bangladesh Medical Journal 38(1) 2009 18-23

scholarly journals Prevalence and correlation of human papillomavirus genotypes with clinical factors in cervical samples from Mexican women

2020 ◽  
pp. 153537022095974
Author(s):  
Fabiola Hernández-Rosas ◽  
Erika Orozco-Hernández ◽  
Liliana Maza-Sánchez ◽  
Pamela Citlalli Salgado-García ◽  
Enrique Navarro-Vidal ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cervical Cancer ◽  
Cancer Screening ◽  
Hpv Infection ◽  
Sexual Partners ◽  
Mexican Women ◽  
Hpv Dna ◽  
Screening Strategies ◽  
Hpv Genotypes ◽  
Cytological Abnormalities

In the last decade, the inclusion of HPV DNA testing in cervical cancer screening has provided one of the best strategies for the prevention and timely detection of HPV. We conducted a high-throughput HPV genotyping study based on MALDI-TOF mass spectrometry to determine the prevalence of 24 HPV genotypes, including oncogenic genotypes, in Mexican women and correlated the results with cytological findings and clinical variables. We likewise identified the risk factors in patients with the HPV infection. Our study included 1000 women from Sonora, Mexico, who participated in cervical cancer screening campaigns and who underwent a Pap smear and HPV DNA test. The results showed that the overall prevalence of HPV was 27.2%, 18.5% with single, and 8.7% multiple infections. The low-risk HPV genotype 6 (8.5%) and oncogenic genotypes 31 (8.1%) and 53 (4.4%) were the most prevalent in the study population. The number of lifetime sexual partners, previous STIs, and age at first intercourse was significantly associated with HPV infection ( P ≤ 0.05). Smoking (OR = 1.5609; 95% IC 1.062–2.292) and more than three lifetime sexual partners (OR = 1.609; 95% IC = 1.124–2.303) represented risk factors for HPV infection. Cytological abnormalities were found in 3.4% of the HPV-positive samples. CIN 1–3 occurred in 0.6% of high-risk HPV cases. In general, the prevalence of the HPV genotypes is high in Mexican women with normal cytological findings. This issue highlights the importance of HPV research in seemingly healthy women and could help guide screening strategies for cervical cancer prevention in Mexico. Impact statement We are submitting data regarding the prevalence and type distribution of the HPV infection and the risk factors associated with it, which may provide a valuable reference to reinforce screening strategies, and to maintain HPV genotype surveillance in Mexico. We discuss the overall prevalence of HPV infection as detected in normal cytological samples stratified by age, different types of infection, and oncogenic capacity. One of the most important findings was that common HPV genotypes detected in healthy women were the genotype numbers: 6, 31, 16, and 56, likewise, smoking and having a history of more than three sexual partners over their lifetime, represented the main risk factors in this study. Furthermore, we found a low frequency of cytological abnormalities and CIN 1–3 in women with HR-HPV.

scholarly journals Risk of cervical cancer associated with HPV infection among the gynae outdoor patients

2014 ◽  
Vol 46 (1-2) ◽  
pp. 3-6
Author(s):  
Tanjila Rahman ◽  
Shahina Tabassum ◽  
Munira Jahan
Keyword(s):  
Risk Factors ◽  
Cervical Cancer ◽  
High Risk ◽  
Sexual Activity ◽  
Hpv Infection ◽  
Early Marriage ◽  
Care Hospital ◽  
Socioeconomic Condition ◽  
Hpv Dna ◽  
Hpv Test

Human papilloma virus is an oncogenic DNA virus, which is identified in 99.7% case of invasive cancer. It is the most prevalent virus involved in sexually transmitted diseases worldwide and an important public health challenge for the prevention of cervical carcinoma. Cervical cancer is the second cause of malignant neoplasia and death in women. The present study was thus designed to detect the risk factors for HPV infection in normal and unhealthy cervix of women attending tertiary care hospital (BSMMU). HPV DNA was detected among the study population using the Hybrid Capture 2 assay, which determined HPV high risk types. A total of 68 women between 25-70 years of age were included in this cross sectional study. Among them 13 (19.1%) cases were tested positive for HPV DNA. The risk factors for HPV infection identified from this result are early sexual activity, lower level of education, early marriage, multi-parity, total duration of sexual activity in years and long term use of oral contraceptive pill and poor socioeconomic condition. Increased awareness and understanding regarding HPV infection would improve the risk of infection and control in sexually active women. HPV diagnosis at early stages of infection is of fundamental importance. The use of HPV test in routine cervical mass screening may not be possible in developing countries like Bangladesh due to its high cost. Nevertheless, it has been shown to be a useful tool when combined with cytology to diagnose high-risk infection in apparently normal tissues, and may help to reduce the risk of cervical cancers. The results obtained from this study ultimately contribute to cervical cancer prevention among Bangladeshi women. DOI: http://dx.doi.org/10.3329/bmjk.v46i1-2.18231 Bang Med J (Khulna) 2013; 46 : 3-6

scholarly journals The correlation of visual inspection, Pap-smear and immunocytochemistry of human papilloma virus in detection of cervical cytology

2016 ◽  
Vol 6 (1) ◽  
pp. 176
Author(s):  
Anuradha Khemka ◽  
Shubhada Jajoo
Keyword(s):  
Cervical Cancer ◽  
Human Papilloma Virus ◽  
Clinical Judgment ◽  
Visual Inspection ◽  
Pap Smear ◽  
Hpv Infection ◽  
Cervical Cytology ◽  
World Health ◽  
Papilloma Virus ◽  
Hpv Dna

Background: Carcinogenicity presents as a major challenge to scientists and society.. Cervical cancer ranks fifth in the world and is the second cause of death in developing countries like India and China. The rate of death due to cervical cancer is greatest in India. At any time women are at risk of harboring HPV infection, which has been seen to cause cervical cancer. Clinical judgment should be used in spite of all high quality modalities available for screening and diagnosis, as initiated by WHO (World health organization) the Down Staging of cervical cancer, VIA, VILI, Pap smear introduced by George Papanicolaou in 1940s , HPV testing by Immunocytochemistry (ICC) and  HPV DNA , Colposcopy , are other modalities to screen cervix. The study aims to evaluate the correlation between Pap smear, visual inspection and Immunocytochemistry of Human papilloma virus (HPV).Methods: All 100 consecutive women were subjected to visual inspection of cervix, Pap smear and immunocytochemistry of HPV. Pap smear was done by conventional method.Results: it was seen that out 100 consecutive cervico-vaginal, ears for Pap and Immunocytochemistry, only 33 were abnormal, 24 were ASCUS, 6 were LSIL, 2 were HSIL, 1 was SCC and 67 were NILM out of which 3 were reactive for ICC. And all 33 were reactive for ICC.Conclusions: The p16 immunostaining performed on conventional smears can become alternative or addition to l HPV DNA tests. The Bethesda System (TBS) 2001 for reporting cervical cytology diagnosis is of high value in detecting abnormal cervical cytology. It is further concluded that the ancillary screening for HPV in NILM category seems to be not advisable as percentage of detection of HPV in this category is low.  

scholarly journals Distribution of high risk Human Papilloma Virus genotypes among cervical cancer patients in a tertiary level hospital in Bangladesh

2019 ◽  
Vol 45 (2) ◽  
pp. 86-92
Author(s):  
Munira Jahan ◽  
Tania Islam ◽  
Sharmin Sultana ◽  
Monira Pervin ◽  
Prof. Ashrafunnessa ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Cancer Patients ◽  
Hpv Infection ◽  
Multiple Infections ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Hpv 18

Background: Cervical cancer is one of the most common types of cancer affecting women worldwide. Persistent HPV infection plays a major role in cervical cancer. The risk of cervical cancer has increased in parallel with the incidence of certain genotypes of human papilloma virus (HPV). Methods: The study was aimed to detect the distribution of HPV genotypes among cervical cancer patients from a specialized hospital in Dhaka, Bangladesh. HPV DNA testing was done by polymerase chain reaction (PCR) using SPF-10 broad-spectrum primers followed by genotyping by reverse hybridization using the INNO-LIPA genotyping system at the Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka. Results: HPV 16 was more prevalent (72.0%) in cervical cancer patients from Bangladesh followed by type 18 (6.0%) and 45 (2.0%). Genotype 16 and 18 alone and as co-infection were detected in 88.0% cases. Multiple HPV infection was found in 20% patients. Along with high risk (HR) HPV 16, 18 and 45 HR HPV 56, 39, 31 and 58 were also prevalent in multiple infections. Conclusion: Thus the study concluded that HR HPV 16 and HPV 18 were more prevalent genotypes among cervical cancer patients in a specialised hospital in Bangladesh. Along with HR HPV 16 and HPV 18, HR HPV 45, 56, 39, 31 and 58 were also prevalent Bangladesh Med Res Counc Bull 2019; 45: 86-92

scholarly journals Prevalence and determinants of high risk human papilloma virus in Hyderabad, India

2018 ◽  
Vol 7 (3) ◽  
pp. 1012 ◽  
Author(s):  
Chandana V. ◽  
Rasheeda Begum Gaguturu
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Age Groups ◽  
Hpv Infection ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Economic Class ◽  
South Central ◽  
One Year

Background: Cervical cancer is the most common cancer among india women and age standardized mortality rate are the highest in south central Asia. In the past decade an among aetiologic association between infection with high risk human papilloma virus and development of cervical cancer has been increased. The study was aimed at studying the prevalence and determinants of high risk human papilloma virus in these patients.Methods: This study was conducted for a period of one year to know the prevalence and determinants of high risk human papilloma virus in the patients attending gynaec out patient department. A total of 266 married women between 15-75 were randomly selected for the study.Results: There is high prevalence of HPV in low (43.3%)/mid (37.4%) socio economic class (17.6%). But the result was not statistically significant (p=0.149). higher prevalence of HPV was found in multiparas (39.4%) compared to multiparas (33.3%) which was not statistically significant (p=0.233). Husbands of 252 women were circumcised out of which 96 wives were positive for HPV DNA (38.1%). Among 14 women with no history of circumcision in their husband HPV DNA positive (28.6%). Correlation of cytology with HPV DNA among different age groups showed increasing prevalence with age but the result was not statistically significant (p=0.115). the result showed that age at marriage, higher the prevalence of HPV DNA. The result was statistically significant (p=0.017). with increasing state of education, the prevalence of HPV infection decreased, but the result was not statically significant (p=0.142).51.3% of women of who were not education were positive for HPV as against only 25% of graduates were HPV positive.Conclusions: Genital hygiene, genetic polymorphisms and other environmental cofactors among HPV DNA positive women, which prevent oncogenesis.

scholarly journals Multicentre, randomised controlled trial of adjuvant chemotherapy in cervical cancer with residual human papilloma virus DNA following primary radiotherapy or chemoradiotherapy: a study protocol

BMJ Open ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. e028171
Author(s):  
Yanhong Wang ◽  
Yi Ouyang ◽  
Jingjing Su ◽  
Jing Liu ◽  
Qunrong Cai ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Adjuvant Chemotherapy ◽  
Randomised Controlled Trial ◽  
Human Papilloma Virus ◽  
Controlled Trial ◽  
Primary Treatment ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Potential Biomarker ◽  
Randomised Controlled

IntroductionThe role of adjuvant chemotherapy after radical radiotherapy (RT) or chemoradiotherapy (CRT) in cervical cancer awaits further confirmation. Evidences have shown that persistent human papilloma virus (HPV) DNA in exfoliated cell post-RT is a potential biomarker of subclinical residual disease and thus increases the risk of recurrence. In this prospective, multicentre, randomised controlled trial, we will use HPV DNA in exfoliated cell to identify patients with cervical cancer who received definitive RT or CRT with higher risk of relapse for adjuvant chemotherapy.Methods and analysisEligible patients with histologically confirmed cervical cancer stage IIA2 to IVA of the International Federation of Gynaecology and Obstetrics, adequate organ function and no locoregional disease or distant metastasis after completion of primary treatment will be screened for HPV DNA in exfoliated cell at 1 month post-RT. Patients with undetectable HPV DNA will undergo standard surveillance. Patients with detectable HPV DNA will be randomly assigned to either adjuvant chemotherapy with docetaxel and nedaplatin for four cycles (arm 1) or observation (arm 2). Patients will be stratified for primary treatment (RT vs CRT). The primary endpoint is relapse-free survival.Ethics and disseminationThis protocol received a favourable ethical opinion from the Ethics Committee of the Second Affiliated Hospital of Fujian Medical University on 6 February, 2018, (No. 28). The trial results will be published in peer-reviewed journals and presented in conferences. A summary of the findings will be made available to participants.Trial registration numberChiCTR-IIR-17012655; Pre-results.

scholarly journals Associations among Serum Lipocalin-2 Concentration, Human Papilloma Virus, and Clinical Stage of Cervical Cancer

Medicina ◽  
2019 ◽  
Vol 55 (6) ◽  
pp. 229 ◽  
Author(s):  
Agnė Vitkauskaitė ◽  
Joana Celiešiūtė ◽  
Saulius Paškauskas ◽  
Erika Skrodenienė ◽  
Rūta Jolanta Nadišauskienė ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papilloma Virus ◽  
Clinical Stage ◽  
Stage Iv ◽  
Hpv Infection ◽  
Control Group ◽  
Lipocalin 2 ◽  
Papilloma Virus ◽  
Tissue Biopsy ◽  
Hpv Types

Background and objective: Lipocalin 2 (LCN2) has an oncogenic role in promoting tumorigenesis through enhancing tumor cell proliferation and the metastatic potential. The aim of our study was to determine whether serum LCN2 could serve as a diagnostic marker of cervical cancer (CC) and to evaluate the correlation between its serum concentration, the clinical stage of the cancer and Human Papilloma Virus HPV infections in women. Materials and methods: A total of 33 women with histologically proven cervical cancer (CC), 9 women with high- grade cervical intraepithelial neoplasia (HSIL) and 48 healthy women (NILM) were involved in the study. A concentration of LCN2 was assayed with the Magnetic LuminexR Assay multiplex kit. An HPV genotyping kit was used for the detection and differentiation of 15 high-risk (HR) HPV types in the liquid-based cytology medium (LBCM) and the tissue biopsy. Results: The majority (84.8%) of the women were infected by HPV16 in the CC group, and there was no woman with HPV16 in the control group (P < 0.01). Several types of HR HPV were found more often in the LBCM compared to in the tissue biopsy (P = 0.044). HPV16 was more frequently detected in the tissue biopsy than the LBCM (P < 0.05). The LCN2 level was higher in HPV-positive than in HPV-negative women (P = 0.029). The LCN2 concentration was significantly higher in women with stage IV than those with stage I CC (P = 0.021). Conclusions: Many HR HPV types, together with HPV16/18, can colonize the vagina and cervix, but often HPV16 alone penetrates into the tissue and causes CC. The serum LCN2 concentration was found to be associated not only with HR HPV infection, irrespective of the degree of cervical intraepithelial changes, but also with advanced clinical CC stage. LCN2 could be used to identify patients with advanced disease, who require a more aggressive treatment.

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