scholarly journals Distribution of high risk Human Papilloma Virus genotypes among cervical cancer patients in a tertiary level hospital in Bangladesh

2019 ◽  
Vol 45 (2) ◽  
pp. 86-92
Author(s):  
Munira Jahan ◽  
Tania Islam ◽  
Sharmin Sultana ◽  
Monira Pervin ◽  
Prof. Ashrafunnessa ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Cancer Patients ◽  
Hpv Infection ◽  
Multiple Infections ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Hpv 18

Background: Cervical cancer is one of the most common types of cancer affecting women worldwide. Persistent HPV infection plays a major role in cervical cancer. The risk of cervical cancer has increased in parallel with the incidence of certain genotypes of human papilloma virus (HPV). Methods: The study was aimed to detect the distribution of HPV genotypes among cervical cancer patients from a specialized hospital in Dhaka, Bangladesh. HPV DNA testing was done by polymerase chain reaction (PCR) using SPF-10 broad-spectrum primers followed by genotyping by reverse hybridization using the INNO-LIPA genotyping system at the Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka. Results: HPV 16 was more prevalent (72.0%) in cervical cancer patients from Bangladesh followed by type 18 (6.0%) and 45 (2.0%). Genotype 16 and 18 alone and as co-infection were detected in 88.0% cases. Multiple HPV infection was found in 20% patients. Along with high risk (HR) HPV 16, 18 and 45 HR HPV 56, 39, 31 and 58 were also prevalent in multiple infections. Conclusion: Thus the study concluded that HR HPV 16 and HPV 18 were more prevalent genotypes among cervical cancer patients in a specialised hospital in Bangladesh. Along with HR HPV 16 and HPV 18, HR HPV 45, 56, 39, 31 and 58 were also prevalent Bangladesh Med Res Counc Bull 2019; 45: 86-92

scholarly journals Genotypes distribution of human papillomavirus in cervical samples of Ecuadorian women

2016 ◽  
Vol 19 (1) ◽  
pp. 160-166 ◽  
Author(s):  
Gustavo David García Muentes ◽  
Lindsay Karen García Rodríguez ◽  
Ramiro Israel Burgos Galarraga ◽  
Franklin Almeida Carpio ◽  
Juan Carlos Ruiz Cabezas
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Cancer Screening ◽  
Cervical Cancer Screening ◽  
Gynecological Cancer ◽  
Hpv Infection ◽  
Multiple Infections ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Hpv 18

ABSTRACT: Introduction: Human papillomavirus (HPV) is considered a necessary causative agent for developing oropharyngeal, anal and cervical cancer. Among women in Ecuadorian population, cervical cancer ranks as the second most common gynecological cancer. Not many studies about HPV burden have been published in Ecuador, and genotypes distribution has not been established yet. The little data available suggest the presence of other genotypes different than 16 and 18. Objectives: In the present study, we attempt to estimate the prevalence of HPV 16, HPV 18 and other 35 genotypes among Ecuadorian women undergoing cervical cancer screening. The overall prevalence of HPV infection was also estimated. Methods: Routine cervical samples were analyzed using Linear Array(r) HPV Genotyping test (Roche). Results: A total of 1,581 cervical samples obtained from Ecuadorian women undergoing cervical cancer screening were included in this study. HPV DNA was detected in 689 cervical samples (43.58%). Of these samples, 604 (38.20%) were positive for a single HPV genotype, while another 85 (5.37%) samples were positive for multiple HPV types. Genotype 16 (5.50%) resulted in the most frequently detected type in both single and multiple infections. HPV 33 (4.55%) and HPV 11 (3.80%) occupied the second and the third place in frequency among all detected genotypes. Conclusions: Viral genotypes different from HPV 16 and HPV 18 are frequently detected among Ecuadorian women. The overall prevalence of HPV resulted higher than the one reported in other South American countries with a greater burden in the second and third decades of life.

scholarly journals Human Papilloma Virus Types 16/18 Distribution in Invasive Cervical Cancer: An Evidence for Vaccination in Bihar, India

2021 ◽  
pp. 59-68
Author(s):  
Rahul Kumar ◽  
Vinita Trivedi ◽  
Richa Chauhan ◽  
Akhtar Parwez ◽  
Biplab Pal ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Vaccination Program ◽  
Hpv 16 ◽  
Tissue Samples ◽  
Papilloma Virus ◽  
Cancer Tissues ◽  
High Risk Hpv ◽  
Hpv 18

There is high incidence of cervical cancer in Bihar, India. Vaccination for cervical cancer in developed countries has played a crucial role in limiting the incidence rate of cervical cancer worldwide. In consideration of debate on clinical efficacy of Human Papilloma Virus (HPV) vaccine in India, study on the prevalence of high risk HPV 16/18 strains in different regions of the nation becomes very crucial. Few individual states have started vaccination but centralised vaccination program does not exist due to lack of sufficient genotypic study of Human Papilloma Virus in different parts of India. Bihar is the third most populous state of India and HPV 16/18 distribution has not been reported yet. The nationwide data of HPV 16/18will help to develop a unified centralised vaccination program. We carried out a distribution study of high risk HPV type 16 and 18 in cervical cancer patients attending a tertiary care hospital of Bihar, India.HPV 16/18 types were analysed in cervical cancer tissues (n = 96) of patients attending the regional cancer hospital of Bihar. Tissue samples were analysed for HPV 16 and HPV 18 using a Real Time PCR technique. The results suggest very high prevalence of HPV 16/18. HPV was identified in all the samples (96/96). About, 74 (77.08%) samples presented with HPV 16 whereas, 16 (16.67%) of the samples presented with HPV 18. 6 Co-infection was presented in 6 (6.25%) of the samples of cervical cancer tissues. HPV 16/18 prevalence is more in the women aged between 41 to 61 years.We report 100% prevalence of HPV16/18 in the cervical cancer tissue samples. A way to minimise this gynaecological concern would be to introduce prophylactic vaccines and early screening in the state of Bihar. The data generated would be crucial in drafting for community screening of HPV. We strongly emphasize the prophylactic HPV Vaccination against HPV 16 to control the alarming rate of cervical cancer in one of the most populous state of India, Bihar.

scholarly journals Prevalence and determinants of high risk human papilloma virus in Hyderabad, India

2018 ◽  
Vol 7 (3) ◽  
pp. 1012 ◽  
Author(s):  
Chandana V. ◽  
Rasheeda Begum Gaguturu
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Age Groups ◽  
Hpv Infection ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Economic Class ◽  
South Central ◽  
One Year

Background: Cervical cancer is the most common cancer among india women and age standardized mortality rate are the highest in south central Asia. In the past decade an among aetiologic association between infection with high risk human papilloma virus and development of cervical cancer has been increased. The study was aimed at studying the prevalence and determinants of high risk human papilloma virus in these patients.Methods: This study was conducted for a period of one year to know the prevalence and determinants of high risk human papilloma virus in the patients attending gynaec out patient department. A total of 266 married women between 15-75 were randomly selected for the study.Results: There is high prevalence of HPV in low (43.3%)/mid (37.4%) socio economic class (17.6%). But the result was not statistically significant (p=0.149). higher prevalence of HPV was found in multiparas (39.4%) compared to multiparas (33.3%) which was not statistically significant (p=0.233). Husbands of 252 women were circumcised out of which 96 wives were positive for HPV DNA (38.1%). Among 14 women with no history of circumcision in their husband HPV DNA positive (28.6%). Correlation of cytology with HPV DNA among different age groups showed increasing prevalence with age but the result was not statistically significant (p=0.115). the result showed that age at marriage, higher the prevalence of HPV DNA. The result was statistically significant (p=0.017). with increasing state of education, the prevalence of HPV infection decreased, but the result was not statically significant (p=0.142).51.3% of women of who were not education were positive for HPV as against only 25% of graduates were HPV positive.Conclusions: Genital hygiene, genetic polymorphisms and other environmental cofactors among HPV DNA positive women, which prevent oncogenesis.

scholarly journals High-risk human papilloma virus infection and cervical neoplasm in female inflammatory bowel disease patients: a cross-sectional study

2019 ◽  
Vol 7 (5) ◽  
pp. 338-344
Author(s):  
Miao Li ◽  
Qing-Fan Yang ◽  
Qian Cao ◽  
Jian Tang ◽  
Yu Gao ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Inflammatory Bowel ◽  
High Risk Hpv

Abstract Background and aim This cross-sectional study investigated the prevalence and risk factors of high-risk human papilloma virus (HPV) infection, especially types 16 and 18, and cervical neoplasia in female Inflammatory bowel disease (IBD) patients. Methods From July 2014 to January 2017, sexually active, female, Chinese IBD patients (21–60 years) and age-matched controls underwent cervical ThinPrep cytology testing (TCT) and high-risk HPV-DNA detection, and completed questionnaires about awareness of cervical cancer and HPV. Cervical dysplasia was categorized as cervical intraepithelial neoplasia (CIN) 1, 2 and 3. Results Of 124 IBD patients (30 ulcerative colitis and 94 Crohn’s disease), 17 (13.7%) had high-risk HPV among whom 9 (7.3%) had HPV 16/18 infection and 4 (3.2%) had cervical CIN (3 CIN 3, 1 CIN 1) by pathology. Among 372 controls, 33 (8.9%) had high-risk HPV and only 1 (0.3%) had HPV 16 infection. Cervical TCT detected atypical squamous cells of unknown significance in one control; no control had CIN. The HPV 16/18 infection rate and CIN prevalence were significantly higher in IBD patients than controls (both P < 0.001). The HPV-infection rate was higher in patients administered methotrexate [P = 0.005, odds ratio (95% confidence interval) 4.76 (1.471–15.402)] or more than two immunosuppressants [P = 0.013, odds ratio (95% confidence interval) 3.64 (1.255–10.562)]. Thiopurine, steroid, infliximab and disease behavior/location were not associated with HPV infection. Only 29.3% of patients had undergone cervical-cancer screening. Awareness of HPV infection and HPV-related cervical cancer was poor (28.2%). Conclusions Female IBD patients are at increased risk of high-risk HPV infection and cervical neoplasia, which may be associated with immunosuppressants. Education and routine follow-up with HPV-DNA testing and TCT are recommended, especially in female Chinese IBD patients.

scholarly journals Human Papilloma Virus and Other Risk Factors of Carcinoma Cervix

1970 ◽  
Vol 38 (1) ◽  
pp. 18-23 ◽  
Author(s):  
S Khatun ◽  
Syed Md Akram Hussain ◽  
F Hossain ◽  
A Choudhury
Keyword(s):  
Risk Factors ◽  
Cervical Cancer ◽  
Cancer Screening ◽  
Human Papilloma Virus ◽  
Sexual Activity ◽  
Hpv Infection ◽  
Carcinoma Cervix ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Screening Strategies

Cervical cancer is the most common cancer among women in many developing countries constituting 20%-30% of female cancers. The etiological factors of cervical cancer include environmental, social, sexual and sexually transmitted agents including human papilloma virus (HPV). HPV has identified in 99.7% invasive cancer. Objective of the study was to find out the prevalence of HPV-DNA virus among diagnosed cases of preinvasive and invasive diseases and to find out different sociodemographic parameters related to carcinoma cervix. This was a cross sectional study. The study has conducted in the department of Obstetrics and Gynaecology of Bangabandhu Sheikh Mujib Medical University. Duration of study was from May 2005 to May 2006. Samples were collected from diagnosed cases of CIN I, II, III and CIS and invasive cancer. Thirty cases were recruited for collection of cervical scraping. Sample were collected of in a specially designed sampler containing liquid transport medium and preserved in minus 20 Degree Celsius and sent to the DNA laboratory for processing and identification of HPV by hybrid capture II method. The results were analyzed by using SPSS version 11.0 and shown in tables and diagrams. The risk factors for HPV infection identified from the result. These are early sexual activity, years of sexual activity, urban people, and higher number of pregnancies. The test could identify HPV-DNA in 100 % of invasive and 50 % of preinvasive cancer. In this study HPV infection was found as a strong determinant for the development of cervical carcinoma and its precursors. Poor socio-economic condition, lower level of education, early marriage and first delivery, multi-parity, years of sexual activity are the risk factors for developing HPV infection. New guidelines for cervical cancer screening strategies could be able to develop from the information obtained from this study. A population-based study with larger sample will be required for the evaluation of cervical cancer screening strategies. Key words: HPV, Carcinoma Cervix   DOI: 10.3329/bmj.v38i1.3582 Bangladesh Medical Journal 38(1) 2009 18-23

Potential coverage of circulating HPV types by current and developing vaccines in a group of women in Bosnia and Herzegovina with abnormal Pap smears

2015 ◽  
Vol 143 (12) ◽  
pp. 2604-2612
Author(s):  
I. SALIMOVIĆ-BEŠIĆ ◽  
M. HUKIĆ
Keyword(s):  
High Risk ◽  
Bosnia And Herzegovina ◽  
Pap Smear ◽  
Multiple Infections ◽  
Pap Smears ◽  
Abnormal Cytology ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Cervical Disease ◽  
Hpv 18

SUMMARYThe objectives of this study were to identify human papillomavirus (HPV) genotypes in a group of Bosnian-Herzegovinian women with abnormal cytology and to assess their potential coverage by vaccines. HPVs were identified by multiplex real-time PCR test (HPV High Risk Typing Real-TM; Sacace Biotechnologies, Italy) of 105 women with an abnormal cervical Pap smear and positive high-risk (HR) HPV DNA screening test. The most common genotypes in the study were HPV-16 (32·6%, 48/147), HPV-31 (14·3%, 21/147), HPV-51 (9·5%, 14/147) and HPV-18 (7·5%, 11/147). The overall frequency of HR HPV-16 and/or HPV-18, covered by currently available vaccines [Gardasil® (Merck & Co., USA) and Cervarix®; (GlaxoSmithKline, UK)] was lower than the overall frequency of other HPVs detected in the study (40·1%, 59/174,P= 0·017). Group prevalence of HR HPVs targeted by a nine-valent vaccine in development (code-named V503) was higher than total frequency of other HPVs detected (68·0%, 100/147,P< 0·001). Development of cervical cytological abnormalities was independent of the presence of multiple infections (χ2= 0·598,P= 0·741). Compared to other HPVs, dependence of cervical diagnosis and HPV-16, -18 (P= 0·008) and HPV-16, -18, -31 (P= 0·008) infections were observed. Vaccines targeting HR HPV-16, -18 and -31 might be an important tool in the prevention of cervical disease in Bosnia and Herzegovina.

scholarly journals Inveksi Human Papilloma Virus (HPV) dan Pencegahannya pada Remaja dan Dewasa Muda

2018 ◽  
Vol 2 (2) ◽  
pp. 81-87
Author(s):  
Agnes S. Rahayu
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Precancerous Lesions ◽  
Invasive Cervical Cancer ◽  
Hpv Infection ◽  
Adolescent And Young Adult ◽  
Adult Women ◽  
Papilloma Virus ◽  
Protection Against Infection

Human papillomavirus (HPV) is a significant source of morbidity and mortality worldwide. The primary risk factors for aquiring HPV are generally associated with sexual activity.  Evidence suggest that condoms provide some protection against infection and disease progression, but any genital contact is sufficient for HPV transmission.  Having more than one sexual partner often result in HPV infection.  All sexually active adolescents are at high risk for aquiring HPV.  Persistent infection with high-risk HPV types (e.g HPV 16 or 18) is considered necessary for the development cervical cancer.  Most infection are asymptomatic and are efficiently cleared by he immune system.  The lesions that caused by HPV can regress in adolescent and young adult women.  A small percentage of adolescents will develop precancerous lesions that may progress to invasive cervical cancer.  Adolescents should be given appropriate education about HPV and the dangers associated with infection.  Vaccination for HPV infection should be given for presexually active children and adolescents.Key words: human papilloma virus, adolescent, cervical cancer, vaccination. 

scholarly journals Μελέτη της λοίμωξης από τους ιούς Epstein-Barr (EBV), ΒΚ (BKV) και του ιού του θηλώματος (HPV) στον θυρεοειδή αδένα και ο ρόλος της στην ανάπτυξη καρκίνου του θυρεοειδούς

2015 ◽  
Author(s):  
Δημήτριος Σταματίου
Keyword(s):  
High Risk ◽  
Human Papilloma Virus ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Epstein Barr ◽  
Hpv 18

Παρά το γεγονός ότι οι ιοί έχουν πρόσφατα εμπλακεί στη ρύθμιση της επιθηλιο-μεσεγχυματικής μετάβασης και στην πρόοδο της καρκινογένεσης, πολύ λίγα είναι γνωστά σχετικά με τη συμμετοχή των ιογενών λοιμώξεων στις κακοήθειες του θυρεοειδούς αδένα. Σκοπός της παρούσας μελέτης ήταν η ανίχνευση αλληλουχιών τριών δυνητικά καρκινογόνων ιών, του BKV, του EBV και του HPV σε μια σειρά δειγμάτων θυρεοειδεκτομής.Ο ιός Epstein-Barr (EBV) ανήκει στην ομάδα των ερπητοϊών και προκαλεί τη λοιμώδη μονοπυρήνωση. Έχει επίσης αναγνωριστεί ως παράγοντας στην ανάπτυξη διαφόρων νεοπλασιών, όπως το λέμφωμα Burkitt, το ρινοφαρυγγικό καρκίνωμα, και άλλες λεμφοβλαστικές διαταραχές σε ανοσοκατεσταλμένα άτομα, γεγονός που υποδεικνύει ότι ο EBV πιθανότατα παίζει σημαντικό ρόλο στη διαδικασία της καρκινογένεσης. Όσον αφορά το θυρεοειδή αδένα, υπάρχουν αρκετές ενδείξεις ότι ο EBV σχετίζεται με την ανάπτυξη κακοήθους λεμφώματος του θυρεοειδούς αλλά και με την εξαλλαγή του διαφοροποιημένου θυρεοειδικού καρκινώματος στην πιο κακοήθη αδιαφοροποίητη μορφή του. Ο ιός BK (BKV) ανήκει στο γένος των polyoma ιών και προκαλεί αιμορραγική κυστίτιδα σε ανοσοκατεσταλμένους ασθενείς, ενώ έχει ανιχνευθεί σ’ένα σημαντικό αριθμό κακοηθειών. Ο ιός Simian Virus 40 (SV40), ένα άλλο μέλος της οικογένειας των polyoma ιών, ξενιστής του οποίου είναι ο ασιατικός ρέζους πίθηκος, έχει εμπλακεί στην καρκινογένεση σε τρωκτικά, ενώ έχει ανιχνευθεί σε δείγματα τόσο θυρεοειδικού καρκίνου, όσο και σε καλοήθη θυρεοειδικά οζίδια.Ο ιός του θηλώματος (Human Papilloma Virus-HPV) ανήκει στο γένος των papilloma ιών. Υπάρχουν πολλοί τύποι HPV, οι οποίοι, εκτός από το ότι προκαλούν διαφόρων ειδών θηλώματα σε διαφορετικά σημεία του σώματος, ανάλογα με τον τύπο, έχει βρεθεί ότι σχετίζονται ιδιαίτερα με την ανάπτυξη καρκίνων. Οι τύποι HPV-16 και HPV-18 χαρακτηρίζονται ως «υψηλού κινδύνου» (high-risk) όσον αφορά την καρκινογένεση, κυρίως του τραχήλου της μήτρας. Μια πρόσφατη έρευνα έδειξε ότι ο HPV-18 έχει τη δυνατότητα να αλληλεπιδρά με πυρηνικούς υποδοχείς, όπως τους υποδοχείς της θυρεοειδικής ορμόνης, των οιστρογόνων και των ανδρογόνων και να τους ενεργοποιεί ανεξαρτήτως της παρουσίας ή της απουσίας της αντίστοιχης ορμόνης. Η μελέτη περιελάμβανε παρασκευάσματα θυρεοειδεκτομής από τριάντα ασθενείς με οζίδια θυρεοειδούς αδένα μέσα σε μια περίοδο 3 ετών. Η δειγματοληψία περιελάμβανε τόσο οζιδιδιακό, όσο και παρακείμενο φυσιολογικό ιστό από το παρασκεύασμα κάθε ασθενούς. Αλληλουχίες των ιϊκών γονιδίων του BKV (γονίδιο VP1), του EBV (γονίδια LMP1, EBNA2 και EBER1) και του HPV (γονίδιο L1), ενισχύθηκαν με PCR. Τα αποτελέσματα της PCR επιβαιώθηκαν με ανάλυση direct sequencing.Αλληλουχίες του γονιδίου VP1 ανιχνεύθηκαν στο 60% (18/30) των δειγμάτων θυρεοειδικού καρκίνου/πολυοζώδους υπερπλασίας, σε σύγκριση με το 43.3% (13/30) σε αντίστοιχο παρακείμενο φυσιολογικό ιστό. Σε δεκαπέντε από τα τριάντα (50%) δείγματα θυρεοειδικού καρκίνου/πολυοζώδους υπερπλασίας, αναδείχθηκαν αλληλουχίες του γονιδίου LMP1 σε σύγκριση με 46.7% (14/30) σε παρακείμενο φυσιολογικό ιστό. Αλληλουχίες του γονιδίου EBNA2 ανιχνεύθηκαν στο 90% (27/30) των δειγμάτων θυρεοειδικού καρκίνου/πολυοζώδους υπερπλασίας, ενώ στο ίδιο ακριβώς ποσοστό (90%-27/30), ανιχνεύθηκαν και στους αντίστοιχους παρακείμενους φυσιολογικούς ιστούς. Όλα τα δείγματα ήταν αρνητικά για αλληλουχίες του EBER1, καθώς και για DNA του ιού HPV.Η παρούσα μελέτη συνιστά τη “λοίμωξη” από BKV και EBV σαν ένα πρώιμο γεγονός, που προφανώς συμβαίνει σε φυσιολογικό ιστό. Οι αλληλουχίες του LMP1, παρατηρήθηκε ότι ανιχνεύθηκαν συχνότερα στα κακοήθη δείγματα συγκριτικά με τα δείγματα φυσιολογικού ιστού. Άν και τα ευρήματά μας δέν επιτρέπουν την εξαγωγή συμπερασμάτων σχετικά με το δυνητικά ογκογόνο ρόλο των εξετασθέντων ιών ως προς το θυρεοειδή αδένα, συνιστούν ένα μοτίβο συνύπαρξης, ή “ενδημικότητας”. Καθώς ο αριθμός των εξετασθέντων δειγμάτων ήταν μικρός και όλα τα δείγματα προέρχονταν από το ίδιο νοσοκομείο, απαιτούνται περαιτέρω μελέτες ώστε να εκτιμηθούν αυτές οι παρατηρήσεις σε σχέση με τοπικοπεριοχικά χαρακτηριστικά.

scholarly journals Ανίχνευση του ιού Human Papillomavirus (HPV) στον καρκίνο του πνεύμονα

2014 ◽  
Author(s):  
Εμμανουέλα Σαρχιανάκη
Keyword(s):  
Human Papillomavirus ◽  
Human Papilloma Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Hpv 18

Παρά το ότι ο ρόλος του ιού των ανθρωπίνων θηλωμάτων ( Human Papilloma virus, HPV) στην εμφάνιση του καρκίνου του τραχήλου της μήτρας είναι καλά τεκμηριωμένος, ο ρόλος του HPV στην καρκινογένεση του πνεύμονα παραμένει αμφιλεγόμενος. Η συχνότητα ανίχνευσης του HPV DNA υπόκεινται σε ευρεία διακύμανση, από 0 έως 100 %. Αυτό εν μέρει οφείλεται στην τεχνική ανίχνευσης που χρησιμοποιήθηκε. Για να διευκρινίσουμε την επίδραση της HPV λοίμωξης στο πνευμονικό παρέγχυμα, αναλύσαμε 100 δείγματα από μη-μικροκυτταρικό καρκίνο πνεύμονα (39 πλακώδη καρκινώματα, 50 αδενοκαρκινώματα, 5 αδενοπλακώδη, 5 αδιαφοροποίητα και 1 μεγαλοκυτταρικό) από την περιοχή της Κρήτης στην Ελλάδα. Ως αρνητικός μάρτυρας χρησιμοποιήθηκαν 16 δείγματα υγειούς πνευμονικού ιστού. Έγινε εκχύλιση DNA από 100 τομές μονιμοποιημένες σε παραφίνη που πάρθηκαν από ασθενείς με μη-μικροκυτταρικό καρκίνο του πνεύμονα. Τα δείγματα εξετάσθηκαν για την παρουσία του HPV DNA με την τεχνική της Real Time PCR χρησιμοποιώντας τους γενικούς εκκινητές GP5+/GP6+. Επιπλέον, τα HPV θετικά δείγματα υπεβλήθησαν σε γονοτυπική ανάλυση. Σε αντιδιαστολή με την απουσία του ιϊκού γονιδιώματος στα δείγματα ελέγχου, HPV DNA ανιχνεύθηκε σε 19 από τα 100 εξετασθέντα δείγματα (19%). Συγκεκριμένα, 4 πλακώδη καρκινώματα, 12 αδενοκαρκινώματα, 1 αδενοπλακώδες και 2 αδιαφοροποίητα ήταν HPV θετικά. Η κατανομή των γονοτύπων του HPV ήταν η ακόλουθη : HPV 16 : 8 περιστατικά (42,1 %) , HPV 11 : 3 περιστατικά (15,8 %) , HPV 6 : 1 περιστατικό (5,2 %) , HPV 33 : 2 περιστατικά (10,5 %) , HPV 31 : 2 περιστατικά (10,5 %) και HPV 18 : 2 περιστατικά (10,5 %) . Η παρουσία του HPV στα δείγματα από καρκίνο παρέχει στοιχεία για τον πιθανό ρόλο του στο μη-μικροκυτταρικό καρκίνο του πνεύμονα και υποδεικνύει την ανάγκη για περαιτέρω έρευνα στο συγκεκριμένο πεδίο.

scholarly journals Results of the first human papilloma virus center in Hungary (2007–2011)

2011 ◽  
Vol 152 (45) ◽  
pp. 1804-1807
Author(s):  
Ádám Galamb ◽  
Attila Pajor ◽  
Zoltán Langmár ◽  
Gábor Sobel
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Hpv Testing ◽  
Papilloma Virus ◽  
Cytological Atypia ◽  
Hpv Dna ◽  
International Data ◽  
Hpv Types ◽  
High Risk Hpv

Human papilloma virus (HPV) is the most common sexually transmitted infection in the 21st century. It has been established that infections with specific HPV types are contributing factors to cervical cancer. Approximately 99.7% of cervical cancers are associated with high risk HPV types. HPV testing plays an important role in the prevention, by decreasing the prevalence and the mortality of cervical cancer. There are 16 HPV-centers operating in Hungary, in which patients undergo HPV screening, cervical exams, and treatment based on standardized guidelines. Patients and methods: The first HPV-center was founded in 2007 in Budapest, at the 2nd Department of Obstetrics and Gynecology, Semmelweis University. This study aimed to define the presence and prevalence of HPV-DNA in the cervical swab samples obtained from patients in our center. Authors conducted to assess the age-specific-prevalence, and HPV type distribution, the associated cervical abnormalities, comparing our results with international data. Results: Overall 1155 woman underwent HPV-testing and genotyping, using polymerase chain reaction. Overall, 55.5% of patients had positive test for HPV DNA types, in which 38.5% for high-risk HPV DNA. Overall prevalence was the highest among females aged 15 to 25years (62.9%). The most common HPV type found was the high risk type 16 (19.5% among the patients with positive HPV testing). Presence of high risk HPV with concurrent cervical cytological abnormality was in 32%. More than two-thirds of woman with cytological atypia (70.6%) were infected with two or more high risk HPV types. HPV 16 was detected in 32% of patients with cytological abnormalities. Conclusions: The results suggest that the prevalence of HPV in this study population exceeds the international data. The results attracts the attention the peak prevalence of the high risk types in the youngest age-group, and the higher risk of cervical abnormality in case of presence of two or more HPV types. The dominance of type 16 and 18 was predictable, but the strong attendance of type 51 and 31 among patients who had cytological atypia, was slightly surprising. Orv. Hetil., 2011, 152, 1804–1807.

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