scholarly journals Antidiabetic and Antioxidant Activities of Decoctions of Coccinia grandis Linn. and Centella asiatica (L.) on Alloxan-induced Diabetic rats

2015 ◽  
Vol 17 (1) ◽  
pp. 86-91 ◽  
Author(s):  
Md Siddiqul Islam ◽  
Salma Parvin ◽  
Md Nasir Uddin ◽  
Md Abdul Mazid
Keyword(s):  
Free Radical ◽  
Fasting Blood Glucose ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Diabetic Rats ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
High Dose ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

The decoctions of Coccinea grandis (L.) and Centella asiatica (L.) leaves were evaluated for their antidiabetic and antioxidant potentials in alloxan-induced rat model and by DPPH free radical scavenging assay, respectively. Graded doses of the decoctions administered to normal and experimental diabetic rats for two weeks showed significant reduction in fasting blood glucose levels in the normal as well as in treated diabetic animals. The experiment showed 188.42 ± 1.2 mg/dl glucose and 117.78 ± 11.2 mg/dl cholesterol levels for C. grandis, while 187.23 ± 0.8 mg/dl glucose and 116.14 ± 1.5 mg/dl cholesterol for C. asiatica. Although, the decoctions produced significant effect on serum urea level, the total protein and liver enzymes (SGOT and SGPT) were found to be normal. Acute toxicity studies showed that both the decoctions were safe at 2-fold high dose when compared with the commonly used antidiabetic dose. Total phenolic contents in decoctions were found to be 12.45±0.52 and 8.32 ± 0.31 for C. grandis and C. asiatica, respectively while DPPH free radical scavenging assay showed the IC50 values of 0.14 ± 0.001 for C. grandis and 0.17± 0.001 for C. asiatica. Our current studies for the first time, justified the use of decoctions of the leaves of C. grandis and C. asiatica for treating diabeties, hyperlipaedemia and atherogenic lesion in the indigenous system of medicine. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22322 Bangladesh Pharmaceutical Journal 17(1): 86-91, 2014

Total Phenolic Content and In vitro Antioxidant Activity of Endophytic Fungi Isolated from Calotropis procera L.

2019 ◽  
Vol 15 (2) ◽  
pp. 232-241 ◽  
Author(s):  
Reena Rani ◽  
Dushyant Sharma ◽  
Monika Chaturvedi ◽  
Jaya Parkash Yadav
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Endophytic Fungi ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Calotropis Procera ◽  
Total Phenolic ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Background: Endophytic fungi are microorganisms that colonize inside the plant tissues without causing any disease symptoms. Endophytic fungi isolated from medicinal plants have emerged as an interesting source for the isolation of bioactive compounds. In this study, we selected Calotropis procera, a member of the Asclepiadaceae family commonly called ‘Aak’ to evaluate the antioxidant potential of isolated endophytic fungi. </P><P> Objective: The aim of this study was to assess the total phenolic content (TPC), antioxidant capacity by using different assay and phytochemical screening of endophytic fungi isolated from Calotropis procera (leaves, stem and root). </P><P> Method: Crude ethyl acetate extracts of 20 different endophytic fungi isolated from Calotropis procera were tested for their preliminary phytoconstituents presence, TPC estimation (by Folin–Ciocalteu colorimetric assay) and antioxidant potential [1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, hydrogen peroxide (H2O2) free radical scavenging assay, β-carotene-linoleic free radical scavenging assay, metal chelating assay and xanthine oxidase inhibitory assay]. Results: Phenols, carbohydrates, saponins, tannins, alkaloids and flavanoids were the main phytoconstituents present in the endophytic fungi. Among the endophytes, Aspergillus nomius showed the highest TPC [72.71±1.67 µg GAE (gallic acid equivalent) /mg dry weight of fungi] and antioxidant activity for DPPH free radical scavenging assay (68.86±0.19%). A high positive linear correlation was found between TPC and xanthine oxidase inhibitory assay (R2-0.890) and between TPC and DPPH free radical scavenging assay (R2-0.839). Aspergillus and Fusarium genus species showed significant antioxidant activity by the different assay. Conclusion: The present study revealed some endophytic fungi from Calotropis procera could be a potential source of novel natural antioxidant compounds.

scholarly journals LC-ESI-QTOF-MS/MS Characterization of Seaweed Phenolics and Their Antioxidant Potential

Marine Drugs ◽  
2020 ◽  
Vol 18 (6) ◽  
pp. 331 ◽  
Author(s):  
Biming Zhong ◽  
Nicholas A. Robinson ◽  
Robyn D. Warner ◽  
Colin J. Barrow ◽  
Frank R. Dunshea ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Polyphenol Content ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Seaweed is an important food widely consumed in Asian countries. Seaweed has a diverse array of bioactive compounds, including dietary fiber, carbohydrate, protein, fatty acid, minerals and polyphenols, which contribute to the health benefits and commercial value of seaweed. Nevertheless, detailed information on polyphenol content in seaweeds is still limited. Therefore, the present work aimed to investigate the phenolic compounds present in eight seaweeds [Chlorophyta (green), Ulva sp., Caulerpa sp. and Codium sp.; Rhodophyta (red), Dasya sp., Grateloupia sp. and Centroceras sp.; Ochrophyta (brown), Ecklonia sp., Sargassum sp.], using liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS). The total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC) were determined. The antioxidant potential of seaweed was assessed using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, a 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging assay and a ferric reducing antioxidant power (FRAP) assay. Brown seaweed species showed the highest total polyphenol content, which correlated with the highest antioxidant potential. The LC-ESI-QTOF-MS/MS tentatively identified a total of 54 phenolic compounds present in the eight seaweeds. The largest number of phenolic compounds were present in Centroceras sp. followed by Ecklonia sp. and Caulerpa sp. Using high-performance liquid chromatography-photodiode array (HPLC-PDA) quantification, the most abundant phenolic compound was p-hydroxybenzoic acid, present in Ulva sp. at 846.083 ± 0.02 μg/g fresh weight. The results obtained indicate the importance of seaweed as a promising source of polyphenols with antioxidant properties, consistent with the health potential of seaweed in food, pharmaceutical and nutraceutical applications.

scholarly journals Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay

Data in Brief ◽  
2018 ◽  
Vol 17 ◽  
pp. 870-875 ◽  
Author(s):  
Kotaro Hara ◽  
Takao Someya ◽  
Katsura Sano ◽  
Yoshimasa Sagane ◽  
Toshihiro Watanabe ◽  
...  
Keyword(s):  
Free Radical ◽  
Sri Lanka ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

In Vitro Regeneration and Free Radical Scavenging Assay of Hypericum perforatum L.

2018 ◽  
Vol 42 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Mohammad Yaseen Mir ◽  
Azra N. Kamili ◽  
Qazi P. Hassan ◽  
Sabreena Rafi ◽  
Javid A. Parray ◽  
...  
Keyword(s):  
Free Radical ◽  
Hypericum Perforatum ◽  
In Vitro Regeneration ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypericum Perforatum L ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

scholarly journals BIOACTIVITY SCREENING OF SELECTED TRADITIONAL MEDICINAL PLANTS OF KERALA

2018 ◽  
Vol 11 (12) ◽  
pp. 339
Author(s):  
Alby Alphons Baby ◽  
Regi Raphael K
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Superoxide Anion ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Significant Activity ◽  
Traditional Use ◽  
Ic50 Value ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Objectives: Traditional medicines all over the world is revaluing nowadays by extensive research programs. To validate the traditional use, the active components in them need to be identified, characterized, and biologically evaluated. Stereospermum suaveolens, Hygrophila spinosa, and Naravelia zeylanica are important medicinal plants used by the ethnic people of Kerala against various ailments. The present study validates the ethnomedical uses of S. suaveolens, H. spinosa, and N. zeylanica by screening their antimicrobial, anthelmintic, and antioxidant properties.Method: All the analyses were done according to standard protocols.Results: The ethanolic extracts of their useful parts were investigated for antimicrobial activity against 10 human pathogenic microorganisms. All the three plants had shown prominent antimicrobial activities, and S. suaveolens exhibits comparatively more antifungal activity in their higher concentration (500 μg/mL). Anthelmintic efficiency of the plants was screened using Indian adult earthworm Pheretima posthuma. All of them had shown significant activity, and the highest was observed in S. suaveolens leaves. Antioxidant potential of the plants was screened using 2,2 diphenyl 1-picrylhydrazyl (DPPH) free radical scavenging assay and superoxide anion scavenging assay. In DPPH free radical scavenging assay, maximum radical scavenging was shown by S. suaveolens with IC50 value 61.6±2.3 μg/mL, and in superoxide anion scavenging assay, maximum activity was in N. zeylanica with IC50 value of 74.66±8.5 μg/mL.Conclusion: This study provides scientific evidence on the traditional use of S. suaveolens (leaves), H. spinosa (leaves), and N. zeylanica (aerial part) in treating microbial diseases, worm disturbances, and their potential as an antioxidant agent.

scholarly journals Phytochemical and Pharmacological Evaluation of Cyperus odoratus Extract

2018 ◽  
Vol 21 (2) ◽  
pp. 150-159
Author(s):  
Ajijul Hakim Alif ◽  
Amir Hossain ◽  
Md Akbar Hossain ◽  
Tabitha Mitali Madhu ◽  
Salma Akter Sumi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Free Radical ◽  
Brine Shrimp ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Free Radical Scavenging ◽  
Biological Data ◽  
Cytotoxic Activities ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Cyperus odoratus (Family: Cyperaceae) is a 10 to 50 cm long perennial herb used as traditional medicinal plant in Bangladesh to treat various pathological conditions like piles, tumour and wound. The ethanolic extract of C. odoratus (COET) has been evaluated for different bioactivities, for example, antioxidant, analgesic, anthelmintic and cytotoxic activities. Antioxidant activity was investigated by DPPH free radical scavenging assay, analgesic activity was evaluated from ‘acetic acid induced writhing inhibition test’ in mice, anthelmintic activities was tested by ‘time of paralysis and death’ assays and cytotoxic activities was observed from the ‘brine shrimp lethality bioassay’. Total tannin and phenol contents were also measured by using Folin-Ciocalteu reagent. Phytochemical screening indicated that the presence of alkaloids, tannins, flavonoids, glycosides, proteins, gums, steroids and acidic compounds. In DPPH free radical scavenging assay the extract showed IC50 value of 8.05 μg/ml, whereas the standard ascorbic acid showed 31.40 μg/ml. The total tannin and phenolic contents were found to be 3.35 and 1.68 mg GAE/100 g of dried extract, respectively. In acetic acid induced writhing assay, the extract showed 36.23 and 61.32% writhing inhibition at doses of 250 mg/kg and 500 mg/kg body weight, respectively, whereas standard Diclofenac-Na (25 mg/kg) showed 70.03% writhing inhibition. The plant extract showed anthelmintic activity in a dose dependant manner. COET also showed LD50 against brine shrimp nauplii at 11.344 μg/mL. After observing all preliminary biological data, it has been confirmed that the plant has antioxidant, analgesic, cytotoxic and anthelmintic properties.Bangladesh Pharmaceutical Journal 21(2): 150-159, 2018

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