Efficient plant regeneration from embryogenic cell suspension culture of two deepwater rice (Oryza sativa L.) Varieties

Cell suspension cultures were initiated from 28-30 d old scutellar-derived embryogenic calli of two deepwater rice varieties HAJA-1 and HAJA-8 and maintained on R2 liquid medium containing 2 mg l-1 2,4-D. The formation of fine embryogenic cell suspensions and subsequent plant regeneration were dependent on plant genotype and culture media. Variety HAJA-8 was found less viable than the other variety HAJA-1. In general, it was observed that variety HAJA-1 showed better performance compared to variety HAJA-8 to R2 medium for the initiation of embryogenic cell lines and the rate of the cell growth was higher in this variety. Plant regeneration was not obtained from cell suspensions of both varieties when cells were cultured on regeneration media semi-solidified with 0.4% (w/v) agarose. Plant regeneration from cell suspensions of the both varieties were obtained when the agrose concentration of the regeneration media was increased from 0.4 to 1% (w/v). The highest plant regeneration frequencies were obtained from cell suspensions of the varieties HAJA-1 and HAJA-8 (48%) and (42%), respectively, pre-treated with water for 5 h and cultured on MS medium supplemented with 2 mg l-1 BAP+1 mg l-1 Proline. Washing cell suspensions with water for a prolonged period of time (15 h and more) inhibited plant regeneration. DOI: http://dx.doi.org/10.3329/cujbs.v5i1.13374 The Chittagong Univ. J. B. Sci.,Vol. 5(1 &2):91-103, 2010

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Phytochemical Analysis and Establishment of Embryogenic Cell Suspension and Agrobacterium-mediated Transformation for Farmer Preferred Cultivars of West African Plantain (Musa spp.)

Plants ◽

10.3390/plants9060789 ◽

2020 ◽

Vol 9 (6) ◽

pp. 789 ◽

Cited By ~ 1

Author(s):

Temitope Jekayinoluwa ◽

Jaindra Nath Tripathi ◽

George Obiero ◽

Edward Muge ◽

Leena Tripathi

Keyword(s):

Cell Suspension ◽

Culture Media ◽

Crop Improvement ◽

Genotypic Variation ◽

Cell Suspensions ◽

Phytochemical Analysis ◽

Embryogenic Calli ◽

Embryogenic Cell ◽

Agrobacterium Mediated Transformation

Banana and plantain are among the foremost staple food crops providing food and livelihood to over 500 million people in tropical countries. Despite the importance, their production is hampered due to several biotic and abiotic stresses. Plant tissue culture techniques such as somatic embryogenesis and genetic transformation offer a valuable tool for genetic improvement. Identification and quantification of phytochemicals found in banana and plantain are essential in optimizing in vitro activities for crop improvement. Total antioxidants, phenolics, flavonoids, and tannins were quantified in various explants obtained from the field, as well as in vitro plants of banana and plantain cultivars. The result showed genotypic variation in the phytochemicals of selected cultivars. The embryogenic cell suspensions were developed for three farmer-preferred plantain cultivars, Agbagba, Obino l’Ewai, and Orishele, using different MS and B5-based culture media. Both culture media supported the development of friable embryogenic calli (FEC), while MS culture media supported the proliferation of fine cell suspension in liquid culture media. The percentage of FEC generated for Agbagba, Obino l’Ewai, and Orishele were 22 ± 24%, 13 ± 28%, and 9 ± 16%, respectively. Cell suspensions produced from FECs were successfully transformed by Agrobacterium-mediated transformation with reporter gene constructs and regenerated into whole plants.

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Evaluation of a plant regeneration test of embryogenic cell suspension cultures of rice (Oryza sativa L. cv Taipei 309)

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-001-0115-x ◽

2001 ◽

Vol 37 (5) ◽

pp. 658-666 ◽

Cited By ~ 1

Author(s):

Alfora-Stella González-coronel ◽

Barnabas Jenes

Keyword(s):

Oryza Sativa ◽

Plant Regeneration ◽

Cell Suspension ◽

Oryza Sativa L ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Embryogenic Cell Suspension ◽

Embryogenic Cell

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Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

Canadian Journal of Botany ◽

10.1139/b91-283 ◽

1991 ◽

Vol 69 (10) ◽

pp. 2257-2260 ◽

Cited By ~ 4

Author(s):

Ann Francine Greer ◽

Zohreh Tabaeizadeh

Keyword(s):

Plant Regeneration ◽

Cell Suspension ◽

Casein Hydrolysate ◽

Leaf Explants ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Cell Suspensions ◽

Lag Phase ◽

Petiole Explants ◽

Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

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