Construction of a Linkage Map and QTL analysis for Black Rot Resistance in Brassica oleracea L.

1970 ◽  
Vol 1 (1) ◽  
pp. 1-6 ◽  
Author(s):  
MAU Doullah ◽  
GM Mohsin ◽  
K Ishikawa ◽  
H Hori ◽  
K Okazaki
Keyword(s):  
Linkage Map ◽  
Brassica Oleracea ◽  
Phenotypic Variation ◽  
Xanthomonas Campestris ◽  
Resistance Breeding ◽  
Black Rot ◽  
Significant Qtls ◽  
Disease Rating ◽  
Genomic Regions ◽  
Xanthomonas Campestris Pv Campestris

For quantitative trait loci (QTL) controlling resistance to Xanthomonas campestris pv. Campestris, we constructed linkage map using cleaved amplified plymorphic sequences (CAPS) and sequence-related amplified polymorphism (SRAP) analysis with disease rating of F3 families obtained from a susceptible broccoli and resistant cabbage [Green commet P09 × Reiho P01]. We established inoculation technique. In this technique, leaves from approximately 50-day old F3 plants were inoculated by cutting 1.0 cm at mid vain near the margins. A total of 38 CAPS and 60 SRAP primer pairs were screened to assess parental polymorphism against black rot resistance. Ninety two markers were distributed in 10 linkage groups (LGs) covering 320.5 cM (centimorgan), with average 3.56 cM interval between markers. Two genomic regions on LG 2 and LG 9 were significantly associated with resistance to the disease. The analysis revealed QTLs in the map interval between CAM1 – GSA1 on LG 2 accounting for up to 10% of the phenotypic variation and one QTL in the map interval between F12-R12e – BORED on LG 9 explaining 16% phenotypic variation with LOD score of 3.09. Two additional non-significant QTLs on LG 3 in the interval between CHI – ASB1 (LOD = 2.04) and on LG 7 in the interval between IPI – FLC3 (LOD = 2.25) were also detected for resistance to the disease. The QTLs, which were mapped to LG 2 and LG 9 for the disease, could be useful for marker-assisted selection in resistance breeding. Key words: Linkage map; QTL; Black rot; Resistance; Brassica oleracea  DOI: http://dx.doi.org/10.3329/ijns.v1i1.8591 International Journal of Natural Sciences (2011), 1(1):1-6

scholarly journals Molecular marking in breeding Brassica oleracea L. for resistance to Xanthomonas campestris pv. campestris

2021 ◽  
Vol 285 ◽  
pp. 03009
Author(s):  
Yuliya Makukha ◽  
Elena Dubina
Keyword(s):  
Brassica Oleracea ◽  
Ssr Markers ◽  
Microsatellite Loci ◽  
Xanthomonas Campestris ◽  
Black Rot ◽  
White Cabbage ◽  
Allelic State ◽  
Xanthomonas Campestris Pv Campestris ◽  
Segregating Population ◽  
Brassica Oleracea L

In the presented study, we have studied polymorphism of 30 microsatellite loci in terms of resistance to black rot (Xanthomonas campestris). Three SSR markers (Ol10-C01, Ol11-H06, BoESSR 726) were identified, showing polymorphism in white cabbage forms with contrasting resistance to black rot. Also, part of the F2 breeding material was analyzed using markers Ol10-C01, Ol11-H06. It was found that they reveal all types of allelic state of genes in a segregating population in accordance with the Mendelian rule of segregation, which makes these markers suitable for further use in studies on the basis of cabbage resistance to black rot.

scholarly journals Early Defense Mechanisms of Brassica oleracea in Response to Attack by Xanthomonas campestris pv. campestris

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2705
Author(s):  
Lu Lu ◽  
Sokrat G. Monakhos ◽  
Yong Pyo Lim ◽  
So Young Yi
Keyword(s):  
Brassica Oleracea ◽  
Defense Mechanisms ◽  
Xanthomonas Campestris ◽  
Radical Scavenging ◽  
Defense Responses ◽  
Black Rot ◽  
Enzymatic Antioxidants ◽  
Induced Expression ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris

Black rot disease, caused by Xanthomonas campestris pv. campestris (Xcc), results in significant yield losses in Brassica oleracea crops worldwide. To find black rot disease-resistant cabbage lines, we carried out pathogenicity assays using the scissor-clipping method in 94 different B. oleracea lines. By comparing the lesion areas, we selected a relatively resistant line, Black rot Resistance 155 (BR155), and a highly susceptible line, SC31. We compared the two cabbage lines for the Xcc-induced expression pattern of 13 defense-related genes. Among them, the Xcc-induced expression level of PR1 and antioxidant-related genes (SOD, POD, APX, Trx H, and CHI) were more than two times higher in BR155 than SC31. Nitroblue tetrazolium (NBT) and diaminobenzidine tetrahydrochloride (DAB) staining analysis showed that BR155 accumulated less Xcc-induced reactive oxygen species (ROS) than did SC31. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays showed that BR155 had higher antioxidant activity than SC31. This study, focused on the defense responses of cabbage during the early biotrophic stage of infection, indicated that Xcc-induced ROS might play a role in black rot disease development. We suggest that non-enzymatic antioxidants are important, particularly in the early defense mechanisms of cabbage against Xcc.

Field evaluation for resistance to the black rot pathogen Xanthomonas campestris pv. campestris in cabbage (Brassica oleracea)

2005 ◽  
Vol 113 (3) ◽  
pp. 297-308 ◽  
Author(s):  
Brita Dahl Jensen ◽  
Said M. S. Massomo ◽  
Ignas S. Swai ◽  
John Hockenhull ◽  
Sven Bode Andersen
Keyword(s):  
Brassica Oleracea ◽  
Xanthomonas Campestris ◽  
Field Evaluation ◽  
Black Rot ◽  
Xanthomonas Campestris Pv Campestris

Vascular stem resistance to black rot in Brassica oleracea

1999 ◽  
Vol 77 (3) ◽  
pp. 442-446 ◽  
Author(s):  
A Ignatov ◽  
Y Kuginuki ◽  
K Hida
Keyword(s):  
Brassica Oleracea ◽  
Xanthomonas Campestris ◽  
Vascular System ◽  
Early Stage ◽  
Dominant Gene ◽  
Black Rot ◽  
Alternative Mechanism ◽  
Stem Resistance ◽  
Systemic Spread ◽  
Xanthomonas Campestris Pv Campestris

The stem vascular system of several accessions of cultivated Brassica oleracea L. showed resistance to black rot caused by Xanthomonas campestris pv. campestris (Pammel) Dowson. Resistance prevented systemic spread of the pathogen in the vascular system of stem. It was race nonspecific and effective at the early stage of seedling growth. A major dominant gene (Rs; resistant in stem), responsible for expression of compete stem resistance in adult plants (more than six true leaves), was found in a Chinese kale line SR1. At least one more dominant gene with additive interaction was necessary to provide complete resistance in seedlings. In progeny of a cross with a cabbage line PI436606, kale SR1 stem resistance segregated independently of race-specific resistance from a PI436606. It can be considered as an alternative mechanism of plant defense, and a highly valuable feature for crops systemically infected by black rot during propagation in commercial nurseries.Key words: black rot, Xanthomonas campestris pv. campestris, Brassica oleracea, disease resistance, cabbage, kale, broccoli.

scholarly journals Genetic Diversity and Population Structure of the Xanthomonas campestris pv. campestris Strains Affecting Cabbages in China Revealed by MLST and Rep-PCR Based Genotyping

2021 ◽  
Vol 37 (5) ◽  
pp. 476-488
Author(s):  
Guo Chen ◽  
Congcong Kong ◽  
Limei Yang ◽  
Mu Zhuang ◽  
Yangyong Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Xanthomonas Campestris ◽  
Resistance Breeding ◽  
Black Rot ◽  
Yield Losses ◽  
Mlst Analysis ◽  
Pathogen Diversity ◽  
Pathogenicity Tests ◽  
Xanthomonas Campestris Pv Campestris

<i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>) is the causal agent of black rot for cruciferous vegetables worldwide, especially for the cole crops such as cabbage and cauliflower. Due to the lack of resistant cabbage cultivars, black rot has brought about considerable yield losses in recent years in China. Understanding of the pathogen features is a key step for disease prevention, however, the pathogen diversity, population structure, and virulence are largely unknown. In this study, we studied 50 <i>Xcc</i> strains including 39 <i>Xcc</i> isolates collected from cabbage in 20 regions across China, using multilocus sequence genotyping (MLST), repetitive DNA sequence-based PCR (rep-PCR), and pathogenicity tests. For MLST analysis, a total of 12 allelic profiles (AP) were generated, among which the largest AP was AP1 containing 32 strains. Further cluster analysis of rep-PCR divided all strains into 14 DNA groups, with the largest group DNA I comprising of 34 strains, most of which also belonged to AP1. Inoculation tests showed that the representative <i>Xcc</i> strains collected from diverse regions performed differential virulence against three brassica hosts compared with races 1 and 4. Interestingly, these results indicated that AP1/DNA I was not only the main pathotype in China, but also a novel group that differed from the previously reported type races in both genotype and virulence. To our knowledge, this is the first extensive genetic diversity survey for <i>Xcc</i> strains in China, which provides evidence for cabbage resistance breeding and opens the gate for further cabbage-<i>Xcc</i> interaction studies.

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