scholarly journals Molecular characterization and morphological clustering of exotic early maturing rice (Oryza sativa L.) lines

2014 ◽  
Vol 11 (2) ◽  
pp. 233-240 ◽  
Author(s):  
MM Sarker ◽  
L Hassan ◽  
MM Rashid ◽  
S Seraj
Keyword(s):  
Oryza Sativa ◽  
Size Range ◽  
Oryza Sativa L ◽  
Gene Diversity ◽  
Allele Size ◽  
Upgma Dendrogram ◽  
Allele Size Range ◽  
Early Maturing ◽  
Positive Correlations ◽  
Simple Sequence

Characterization and variability analysis is important for the improvement of crop plant. This study aimed to evaluate the morphological and molecular variation of exotic early maturing rice (Oryza sativa L.) lines. A total of 32 exotic rice lines collected from different locations were genotyped and clustered using selected SSR markers. Based on morphological dendrogram, the lines were grouped into three clusters viz.I, II and III. Cluster I, cluster II and cluster III had 12, 11, 9 lines respectively. The results showed that the varieties were closely related belonging to the same cluster. DNA Markers namely Simple Sequence Repeats (SSR) is a useful tool for assessing genetic variations and resolving cultivar identities. Positive correlations were found between gene diversity, number of allele, the allele size range and the maximum number of repeats. Among the primers used RM147 identified more number of alleles and average PIC was 0.88. The UPGMA dendrogram based on Nei’s (1972) genetic distance grouped the 32 rice lines into three major clusters. This result indicates that the line which formed grouped together, they are less diverse. A significant level of polymorphism based on morphological and molecular levels was observed. Being grouped into three clusters C1-4-11-7P-2P-1P and IR 79201-49-1-1-1 could be utilized as potential parents for the improvement of yield in early maturing rice lines. DOI: http://dx.doi.org/10.3329/jbau.v11i2.19900 J. Bangladesh Agril. Univ. 11(2): 233-240, 2013

scholarly journals Molecular and Phenotypic Characterization for Cold Tolerance in Rice (Oryza sativa L.)

2020 ◽  
Vol 23 (2) ◽  
pp. 1-15
Author(s):  
PS Biswas ◽  
H Khatun ◽  
M Anisuzzaman
Keyword(s):  
Cold Stress ◽  
Cold Tolerance ◽  
Oryza Sativa L ◽  
Gene Diversity ◽  
Reproductive Stage ◽  
Seedling Stage ◽  
Phenotypic Characterization ◽  
Breeding Lines ◽  
Upgma Dendrogram ◽  
Ssr Loci

Cold stress affects growth and development of rice plants from germination to reproductive stages. Thirty breeding lines/varieties including tolerant and susceptible check varieties were evaluated for cold tolerance at seedling and reproductive stage using artificially induced low temperature situations to understand the genetic relationship among them and to identify promising donors for cold tolerance. Three breeding lines, BR8253-9-3-4-3, IR77497-31-2-3-1and IR2266-42-6-2 demonstrated higher cold tolerance for the seedling stage (LD score <3.0). Molecular analysis revealed 68% genetic similarity among the genotypes. On average 64% of the breeding lines shared common alleles at any given locus ranging from 27% (RM22870) to 97% (RM186). UPGMA dendrogram showed that majority of the breeding lines showing 4-5 degrees of LD were grouped together. Two breeding lines, BR8258-7-1-5-2B2 and BR8258-7-1-5-2B3 having 86% common alleles showed low leaf discoloration (LD: 4) at seedling stage coupled with relatively low reduction in spikelet fertility (45.8% and 48.1%) and grain yield (43.3% and 39.1%). The gene diversity and PIC value revealed that seven SSR loci (RM1282, RM600, RM5349, RM6972, RM5847, RM5911, and RM511) might be the best markers to differentiate between tolerant and susceptible genotypes at reproductive stage. The breeding lines with tolerance to cold stress at the seedling and the reproductive stages identified from this study, and the SSR markers mentioned above could be useful for developing cold tolerance rice. Bangladesh Rice j. 2019, 23(2): 1-15

Morpho-agronomic and simple sequence repeat-based diversity in colored rice (Oryza sativa L.) germplasm from peninsular India

2011 ◽  
Vol 59 (2) ◽  
pp. 179-189 ◽  
Author(s):  
S. J. M. Gowda ◽  
G. J. Randhawa ◽  
I. S. Bisht ◽  
P. K. Firke ◽  
A. K. Singh ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Simple Sequence Repeat ◽  
Oryza Sativa L ◽  
Sequence Repeat ◽  
Peninsular India ◽  
Simple Sequence ◽  
Colored Rice

scholarly journals Screening of some rice Oryza sativa L. genotypes for drought tolerance using SSR-PCR technique

2014 ◽  
Vol 8 (2) ◽  
pp. 14-18
Author(s):  
Asmaa K. Aurabi ◽  
Kadhim M. Ibrahim ◽  
Shatha A. Yousif
Keyword(s):  
Oryza Sativa ◽  
Drought Tolerance ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Oryza Sativa L ◽  
Sequence Repeat ◽  
Drought Tolerant ◽  
Simple Sequence

Simple Sequence Repeat (SSR) markers were used to investigate the variations between drought sensitive and drought tolerant genotypes. Patterns obtained using RM328 and RM302 detected polymorphism between T14, T15 and Amber33, Amber Baghdad genotypes and those obtained using RM316 and RM201 for the tested genotypes suggested that these primers may have the ability to produce drought tolerance markers. According to the patterns obtained using RM189, RM3825 and RM212 primers indicated that these primers cannot be relied on as markers for drought tolerance.

scholarly journals GENETIC VARIATION AND RELATEDNESS AMONG HIGH YIELDING RICE VARIETIES (Oryza sativa L.) REVEALED BY RAPD MARKERS

2008 ◽  
Vol 21 (1) ◽  
pp. 07-14
Author(s):  
F. Easmin ◽  
M. S. Rahman ◽  
M. S. Islam ◽  
M. A. Samad ◽  
M. S. Alam
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Rapd Markers ◽  
Oryza Sativa L ◽  
Gene Diversity ◽  
Chain Reaction ◽  
Rice Varieties ◽  
Upgma Dendrogram ◽  
Polymerase Chain ◽  
High Level

Genetic variation is a principal concern for the plant breeders. Genetic variation and relationship among high yielding rice varieties viz. Binadhan 4, Binadhan 5, Binadhan 6, Binasail, BRRI dhan28 and BRRI dhan29 were analyzed using four decamer random primers. Polymerase Chain Reaction (PCR) amplified 22 RAPD markers, of which 18 (81.82%) were polymorphic. The proportion of polymorphic loci and the gene diversity values were 59.09% and 0.25 for the Binadhan 4; 59.09% and 0.21 for Binadhan 6; 54.55% and 0.23 for Binasail; 54.55% and 0.19 for BRRI dhan29; 50.00% and 0.19 for Binadhan 5 and 45.45% and 0.18 for BRRI dhan28, respectively. The coefficient of gene differentiation (Gst) across all loci was calculated as 0.35 reflecting the existence of high level of genetic variation among the six modern rice varieties. UPGMA dendrogram based on Nei’s genetic distance segregated the six high yielding rice varieties into two clusters: all four mutant varieties viz. Binadhan 4, Binadhan 5, Binadhan 6 and Binasail formed one cluster and two varieties of BRRI grown in boro season, BRRI dhan28 and BRRI dhan29 grouped together in another cluster. Among the mutants, two boro season varieties, developed from the same parent, Binadhan 5 and Binadhan 6 grouped together with genetic distance of 0.10. Therefore, RAPD offer a reliable method to evaluate genetic variation and relatedness among the high yielding rice varieties.DOI: http://dx.doi.org/10.3329/bjpbg.v21i1.17042

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