Phytochemical Action of White Mulberry Extracts against Three Important Stored Grain Pests

Petroleum ether (Pet. ether), CHCl3 and CH3OH extracts of white mulberry were subjected to dose-mortality tests against Callosobruchus chinensis (L.), Sitophilus oryzae (L.) and Tribolium castaneum (Hbst.). Pet. ether extract of leaves against C. chinensis, S. oryzae and T. castaneum provided LD50 9.16, 5.06, 3.79, 2.81, 2.06, 1.55, 1.32, 1.38 mg/g; 17.6, 12.9, 11.3, 82.4, 83.5, 9.47, 8.73, 8.61 mg/g and 0.93, 0.826, 0.691, 0.623, 0.567, 0.532, 0.525 mg/cm2 after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against C. chinensis Pet. ether extract of root and stem bark gave LD50 28.3, 20.7, 18.4, 16.9, 14.4, 13.6, 13.3, 13.3 mg/g and 86.3, 228.9, 177, 109, 61.0, 21.8, 0.999, 15 mg/g after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. CH3OH extracts of leaves offered LD50 21.5, 20.4, 17, 16.2, 15.6, 14.7 mg/g against C. chinensis; 5.4, 14.7, 13.7, 11, 10.9, 9.46, 9.10 mg/g against S. oryzae; and 3.804, 3.429, 3.284, 2.736, 2.441, 2.311, 2.264 mg/cm2 against T. castaneum after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against S. oryzae CH3OH extract of root and CHCl3 of stem bark gave LD50 19.5, 19.1, 17.5, 16.7, 16.2, 15.8, 15.9 mg/g and 9.77, 9.44, 7.71, 6.56, 5.94, 5.39, 5.4 mg/g after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. However, CHCl3 extract of leaves and root didnt show mortality.

Download Full-text

Bioactive Potentials of Cleome viscosa L. Extracts: Dose-mortality, Insect Repellency and Brine Shrimp Lethality

Journal of Scientific Research ◽

10.3329/jsr.v9i4.32592 ◽

2017 ◽

Vol 9 (4) ◽

pp. 375-382

Author(s):

S. A. Rimi ◽

S. Hossain ◽

S. Islam ◽

Z. Islam ◽

S. B. Chhabi ◽

...

Keyword(s):

Petroleum Ether ◽

Tribolium Castaneum ◽

Brine Shrimp ◽

Artemia Salina ◽

Callosobruchus Chinensis ◽

Cytotoxicity Test ◽

Ether Extract ◽

Chcl3 Extract ◽

Level Of Significance ◽

Cleome Viscosa

Petroleum ether (Pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of Cleome viscosa have thoroughly screened against Tribolium castaneum (Hbst.) for repellency and dose mortality, against Callosobruchus chinensis for dose mortality activities, and against Artemia salina nauplii for lethality. In case of repellency of T. castaneum, Pet. ether extract found significantly active at 1% level of significance (P < 0.01), CHCl3 extract offered activity at 5% level of significance (P < 0.05) and CH3OH extract did not show any activity at all. In case of mortality against T. castaneum the LD50 values of Pet. ether, CHCl3, and CH3OH extracts were 2.380, 2.188, 1.867, 1.658 and 1.429 mg/cm2; 4.950, 2.906, 2.664, 2.658 and 2.609 mg/cm2; and 4.950 and 2.791, 2.609, 2.552 and 2.474 mg/cm2 after 0.5, 6, 12, 18 and 24 h of exposure respectively. Against C. chinensis the LD50 values of the same extracts were 0.781, 0.670, 0.642, 0.614, 0.560 and 0.494 mg/cm2; 1.964, 1.050, 0.867, 0.725, 0.635 and 0.590 mg/cm2; and 2.631, 1.851, 1.471, 1.703, 1.524 and 1.457 mg/cm2 after 6, 12, 18, 24, 30 and 36 h of exposure respectively. In case of cytotoxicity test against A. salina, the extracts offered LC50 values in a descending order CH3OH > CHCl3 > Pet. ether extracts.

Download Full-text

Dose-mortality, Cytotoxicity and Repellent Activity of Abutilon hirtum (Lam.) Sweet against Callosobruchus chinensis (L.), Artemia salina L. and Tribolium castaneum (Hbst.)

Journal of Scientific Research ◽

10.3329/jsr.v9i4.32590 ◽

2017 ◽

Vol 9 (4) ◽

pp. 367-373

Author(s):

S. Hossain ◽

S. A. Rimi ◽

H. Ali ◽

R. A. Shawon ◽

M. Abdullah ◽

...

Keyword(s):

Tribolium Castaneum ◽

Artemia Salina ◽

Repellent Activity ◽

Callosobruchus Chinensis ◽

Ether Extract ◽

Stored Grain ◽

Cytotoxic Effects ◽

Aerial Parts ◽

Chcl3 Extract ◽

Level Of Significance

Petroleum ether (Pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of the aerial parts of Abutilon hirtum (Lam.) Sweet were subjected to dose-mortality against the stored grain pest Callosobruchus chinensis (L.), cytotoxicity against brine Shrimp Artemia salina L. nauplii and repellent activity against adult beetles of Tribolium castaneum (Hbst.). Against C. chinensis only CH3OH extract showed promising mortality and provided LD50 values 1.344, 1.294, 1.243 and 1.152 mg/cm2 after 6, 12, 18 and 24 h of exposure respectively, however, Pet. ether and CHCl3 extracts didnt show mortality. Against A. salina nauplii Pet. ether, CHCl3 and CH3OH extracts showed cytotoxic effects; while Pet. ether extract gave LC50 values 2461.031, 642, 191.233, 94.618 ppm after 6, 12, 18 and 24 h of exposure respectively, and CHCl3 extract provided LC50 values 1336.124, 679.387, 276.961 and 199.988 ppm; and CH3OH offered 531.896, 212.840, 91.499 and 72.975 ppm after 6, 12, 18 and 24 h of exposure respectively. For repellency against T. castaneum adults CHCl3 extract showed significant result at 1% level of significance (P < 0.01), while the CH3OH extract showed moderate repellency at 5% level of significance (P < 0.05), but the Pet. ether extract didnt show any significant repellent activity.

Download Full-text

Bioactive Potentials of Cassia renigera Benth. Extracts against the Stored Product Pests Callosobruchus chinensis L., Sitophilus oryzae L. and Tribolium castaneum (Hbst.)

Journal of Scientific Research ◽

10.3329/jsr.v9i4.32588 ◽

2017 ◽

Vol 9 (4) ◽

pp. 351-357 ◽

Cited By ~ 1

Author(s):

M. Nesa ◽

S. Islam ◽

S. K. Sharma ◽

S. B. Rekha ◽

J. Ferdous ◽

...

Keyword(s):

Tribolium Castaneum ◽

Sitophilus Oryzae ◽

Test Organism ◽

Stem Bark ◽

Repellent Activity ◽

Callosobruchus Chinensis ◽

Ether Extract ◽

Leaf Extracts ◽

Level Of Significance ◽

Cassia Renigera

Petroleum (Pet.) ether, CHCl3 and CH3OH extracts of Cassia renigera Benth. were tested against Callosobruchus chinensis L. for dose-mortality and repellency; and against Sitophilus oryzae L. and Tribolium castaneum (Hbst.) for repellent activity test. The Pet. ether extract of leaf was found active against C. chinensis adults through dose mortality assay and the LD50 values established were 9.270, 4.932, 2.032, 1.721, 1.671, 1.543, 1.513, 1.503 and 1.478 mg cm-2 for 0.5, 6, 12, 18, 24, 30, 36, 42 and 48 h of exposure respectively. However, the leaf extracts of CHCl3 and CH3OH; as well as the Pet. ether, CHCl3 and CH3OH extracts of the stem-bark did not give mortality to the test organism. The CHCl3 extract of leaf showed repellent activity against Tribolium castaneum at 0.1% level of significance (P < 0.001), and the Pet. ether extracts of the leaf and the CH3OH extracts of the stem-bark offered repellency at 1% level of significance (P < 0.01) between dose levels. Only the CHCl3 extracts of the stem-bark gave repellency at 5% level of significance (P < 0.05), while the CH3OH extracts of leaf and Pet. ether extract of stem-bark did not show any significant repellency at all.

Download Full-text

Molecular characterization and identification of three stored grain pests based on mitochondrial cytochrome C oxidase subunit I (COI) gene sequences

Bangladesh Journal of Zoology ◽

10.3329/bjz.v47i1.42016 ◽

2019 ◽

Vol 47 (1) ◽

pp. 1-11

Author(s):

Abu Faiz Md Aslam ◽

Sharmin Sultana ◽

Faria Farhana Rain ◽

Sumita Rani Das ◽

Ayesha Siddika ◽

...

Keyword(s):

Insect Pests ◽

Sitophilus Oryzae ◽

Dna Barcode ◽

Control Measures ◽

Coi Gene ◽

Composition Analysis ◽

Callosobruchus Chinensis ◽

Pest Species ◽

Stored Grain ◽

Stored Grain Pests

Stored grain pests are discovered in food as immature stages, which further complicates the identification process. A DNA barcode dataset of some important pests that can be used for easy and confirm identification in stages of life is constructed. COI genes of three stored grain insect pests i.e,, Sitophilus oryzae, Callosobruchus chinensis and Oryzaephilus surinamensis were sequenced. The sequenced genes were submitted to NCBI GenBank and obtained accession numbers MG967331.1, MG967332.1, MG967333.1 and MK041216.1. BLAST analysis showed 99 to 100% homology with existing GenBank sequences. The nucleotide composition analysis revealed that the value of A+T (64.8%) is greater than G+C (35.2%). Genetic distance among four sequences of three store pests were ranged from 0.00293-0.32807. Phylogenetic analysis showed that these three species are originated from different clades. Haplotype analysis of mitochondrial COI gene of the stored grain insect pests showed high genetic diversity among them. C. chinensis, O. surinamensis and S. oryzae were separated from their common ancestor by 80, 73 and 64 mutational steps. These information may be helpful for attempting any successful control measures against the pest species. In conclusion, present author established the first DNA barcode dataset of three store grain pests and confirmed its efficiency for identifying these pests. Bangladesh J. Zool. 47(1): 1-11, 2019

Download Full-text