scholarly journals Bioactive Potentials of Cleome viscosa L. Extracts: Dose-mortality, Insect Repellency and Brine Shrimp Lethality

2017 ◽  
Vol 9 (4) ◽  
pp. 375-382
Author(s):  
S. A. Rimi ◽  
S. Hossain ◽  
S. Islam ◽  
Z. Islam ◽  
S. B. Chhabi ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Tribolium Castaneum ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Callosobruchus Chinensis ◽  
Cytotoxicity Test ◽  
Ether Extract ◽  
Chcl3 Extract ◽  
Level Of Significance ◽  
Cleome Viscosa

Petroleum ether (Pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of Cleome viscosa have thoroughly screened against Tribolium castaneum (Hbst.) for repellency and dose mortality, against Callosobruchus chinensis for dose mortality activities, and against Artemia salina nauplii for lethality. In case of repellency of T. castaneum, Pet. ether extract found significantly active at 1% level of significance (P < 0.01), CHCl3 extract offered activity at 5% level of significance (P < 0.05) and CH3OH extract did not show any activity at all. In case of mortality against T. castaneum  the LD50 values of Pet. ether, CHCl3, and CH3OH extracts were 2.380, 2.188, 1.867, 1.658 and 1.429 mg/cm2; 4.950, 2.906, 2.664, 2.658 and 2.609 mg/cm2; and 4.950 and 2.791, 2.609, 2.552 and 2.474 mg/cm2 after 0.5, 6, 12, 18 and 24 h of exposure respectively.  Against C. chinensis the LD50 values of the same extracts were 0.781, 0.670, 0.642, 0.614, 0.560 and 0.494 mg/cm2; 1.964, 1.050, 0.867, 0.725, 0.635 and 0.590 mg/cm2; and 2.631, 1.851, 1.471, 1.703, 1.524 and 1.457 mg/cm2 after  6, 12, 18, 24, 30 and 36 h of exposure respectively. In case of cytotoxicity test against A. salina, the extracts offered LC50 values in a descending order CH3OH > CHCl3 > Pet. ether extracts.

scholarly journals Dose-mortality, Cytotoxicity and Repellent Activity of Abutilon hirtum (Lam.) Sweet against Callosobruchus chinensis (L.), Artemia salina L. and Tribolium castaneum (Hbst.)

2017 ◽  
Vol 9 (4) ◽  
pp. 367-373
Author(s):  
S. Hossain ◽  
S. A. Rimi ◽  
H. Ali ◽  
R. A. Shawon ◽  
M. Abdullah ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Artemia Salina ◽  
Repellent Activity ◽  
Callosobruchus Chinensis ◽  
Ether Extract ◽  
Stored Grain ◽  
Cytotoxic Effects ◽  
Aerial Parts ◽  
Chcl3 Extract ◽  
Level Of Significance

Petroleum ether (Pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of the aerial parts of Abutilon hirtum (Lam.) Sweet were subjected to dose-mortality against the stored grain pest Callosobruchus chinensis (L.), cytotoxicity against brine Shrimp Artemia salina L. nauplii and repellent activity against adult beetles of Tribolium castaneum (Hbst.). Against C. chinensis only CH3OH extract showed promising mortality and provided LD50 values 1.344, 1.294, 1.243 and 1.152 mg/cm2 after 6, 12, 18 and 24 h of exposure respectively, however, Pet. ether and CHCl3 extracts didn’t show mortality. Against A. salina nauplii Pet. ether, CHCl3 and CH3OH extracts showed cytotoxic effects; while Pet. ether extract gave LC50 values 2461.031, 642, 191.233, 94.618 ppm after 6, 12, 18 and 24 h of exposure respectively, and CHCl3 extract provided LC50 values 1336.124, 679.387, 276.961 and 199.988 ppm; and CH3OH offered 531.896, 212.840, 91.499 and 72.975 ppm after 6, 12, 18 and 24 h of exposure respectively. For repellency against T. castaneum adults CHCl3 extract showed significant result at 1% level of significance (P < 0.01), while the CH3OH extract showed moderate repellency at 5% level of significance (P < 0.05), but the Pet. ether extract didn’t show any significant repellent activity.

scholarly journals Phytochemical Action of White Mulberry Extracts against Three Important Stored Grain Pests

2017 ◽  
Vol 9 (4) ◽  
pp. 359-366
Author(s):  
Z. R. Sabuj ◽  
A. Khatun ◽  
M. Abdullah ◽  
D. M. Badruddoza ◽  
H. Ali ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Tribolium Castaneum ◽  
Sitophilus Oryzae ◽  
Stem Bark ◽  
Callosobruchus Chinensis ◽  
Ether Extract ◽  
Stored Grain ◽  
Chcl3 Extract ◽  
Stored Grain Pests ◽  
White Mulberry

Petroleum ether (Pet. ether), CHCl3 and CH3OH extracts of white mulberry were subjected to dose-mortality tests against Callosobruchus chinensis (L.), Sitophilus oryzae (L.) and Tribolium castaneum (Hbst.). Pet. ether extract of  leaves against C. chinensis, S. oryzae and T. castaneum provided LD50 9.16, 5.06, 3.79, 2.81, 2.06, 1.55, 1.32, 1.38 mg/g; 17.6, 12.9, 11.3, 82.4, 83.5, 9.47, 8.73, 8.61 mg/g and 0.93, 0.826, 0.691, 0.623, 0.567, 0.532, 0.525 mg/cm2 after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against C. chinensis Pet. ether extract of root and stem bark gave LD50 28.3, 20.7, 18.4, 16.9, 14.4, 13.6, 13.3, 13.3 mg/g and 86.3, 228.9, 177, 109, 61.0, 21.8, 0.999, 15 mg/g after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. CH3OH extracts of leaves offered LD50 21.5, 20.4, 17, 16.2, 15.6, 14.7 mg/g against C. chinensis; 5.4, 14.7, 13.7, 11, 10.9, 9.46, 9.10 mg/g against S. oryzae; and 3.804, 3.429, 3.284, 2.736, 2.441, 2.311, 2.264 mg/cm2 against T. castaneum after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against S. oryzae CH3OH extract of root and CHCl3 of stem bark gave LD50 19.5, 19.1, 17.5, 16.7, 16.2, 15.8, 15.9 mg/g and 9.77, 9.44, 7.71, 6.56, 5.94, 5.39, 5.4 mg/g after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. However, CHCl3 extract of leaves and root didn’t show mortality.

scholarly journals Cytotoxicity Assessment of Heritiera Littoralis (Aiton), Madhuca Longifolia (König) Macbr., Nerium Indicum Mill. And Sapium Indicum (Willd.) Leaves on Artemia Salina (L.)

2021 ◽  
Vol 29 (2) ◽  
pp. 139-149
Author(s):  
N Fatema ◽  
K Hasan ◽  
A Islam ◽  
N Yasmin ◽  
M Nesa ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Artemia Salina ◽  
Ether Extract ◽  
Chcl3 Extract ◽  
Madhuca Longifolia ◽  
Cytotoxicity Assessment

Petroleum ether, CHCl3 and CH3OH extracts of leaves of Heritiera littoralis (Aiton), Madhuca longifolia (König) Macbr., Nerium indicum Mill. and Sapium indicum (Willd.) were subjected to assess cytotoxicity against Artemia salina (L.) nauplii. The petroleum ether extract of H. littoralis leaves showed LC50 values 273.77, 97.27, 51.60, 37.12, 14.60 and 12.59 ppm after 12, 18, 24, 30, 36 and 42 h; the CHCl3 extract showed LC50 values 733.25, 105.51, 40.72 and 18.20 ppm after 6, 12, 18 and 24 h whereas CH3OH extract showed 73.05, 30.62, 24.56, 20.85, 16.21 and 6.71 ppm after 6, 12, 18, 24, 30 and 36 h of exposure respectively. The petroleum ether extract of M. longifolia leaves possess LC50 values 259.35, 115.17, 56.84 and 8.73 ppm after 12, 18, 24 and 30 h; the CHCl3 extract possess LC50 values 585.43, 205.86, 112.74, 75.62, 52.84and 47.34 ppm after 12, 18, 24, 30, 36 and 42 h but CH3OH extract possess LC50 values 185.87, 60.70, 30.11 and 15.39 ppm after 12, 18, 24 and 30 h of exposure respectively. The petroleum ether extract of N. Indicum leaves recorded LC50 values 249.82, 146.07, 80.23, 54.21 and 40.19 ppm after 18, 24, 30, 36 and 42 h; the CHCl3 extract gave LC50 values 36.13, 21.72, 19.03, 16.81 and 16.34 ppm after 12, 18, 24, 30 and 36 h but CH3OH extract recorded LC50 values 394.90, 129.69, 81.50, 73.10 and 37.51 ppm after 18, 24, 30, 36 and 42 h of exposure respectively. Similarly, the petroleum ether extract of S. indicum leaves showed LC50 values 24.79, 13.18 and 4.61 ppm after 12, 18 and 24 h; the CHCl3 extract were 50.45, 42.64, 21.20 and 14.93 ppm after 18, 24, 30 and 36 h of exposure and the CH3OH extract showed LC50 values 306.37, 217.18, 149.38, 73.52, 54.45 and 22.91 ppm after 12, 18, 24, 30, 36 and 42 h of exposure respectively. The intensity of efficacy of the extracts could be arranged in the following descending order of S. indicum (petroleum etroleum ether extract) >H. littoralis (CH3OH extract) >M. longifolia (petroleum ether extract) >N. indicum (CHCl3 extract). J. Bio-Sci. 29(2): 139-149, 2021 (December)

scholarly journals Evaluation of Biological Activities of Evolvulus Nummularius L. through Insecticidal, Insect Repellency and Brine Shrimp Lethality Tests

2014 ◽  
Vol 8 ◽  
pp. 101-104
Author(s):  
Md Moniruzzaman ◽  
Nayeema Parvin ◽  
Sabina Sultana ◽  
Mohammad Abdullah ◽  
Ataur Rahman Khan ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Brine Shrimp ◽  
Biological Activities ◽  
Artemia Salina ◽  
Moderate Activity ◽  
Meoh Extract ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Chcl3 Extract ◽  
Brine Shrimp Lethality Assay

Assessment of Evolvulus nummularius L. through whole plant extracts (collected in petroleum ether [Pet.E.], chloroform [CHCl3] and methanol [MeOH]) were made against Tribolium castaneum (Hbst.) for insecticidal and insect repellency and against Artemia salina L. for brine shrimp lethality, and the results were promising. The LC50 values against T. castaneum adults for Pet.E. extract were 1.280, 1.220, 1.096 and 1.030?g cm-2; for CHCl3 extract 3.249, 2.990, 2.526 and 2.204?g cm-2; and for MeOH extract 2.925, 1.899, 1.875 and 1.789?g cm-2, respectively for 12, 24, 36 and 48h of exposure. The intensity of activity could be arranged in a descending order: Pet.E. extract> MeOH extract> CHCl3 extract. For repellency, CHCl3 and MeOH extracts offered moderate activity (P <0.01), but the Pet.E. extract did not show efficacy against the beetles. The same extracts responded positively in brine shrimp lethality assay where the LC50 values for Pet.E. extract were 141.553, 98.941, 24.684 and 8.560ppm; for CHCl3 extracts 699.048, 366.663, 326.0039 and 40.114ppm; and for MeOH extracts 2336.982, 1258.605, 355.962 and 137.0589ppm respectively for 12, 18, 24 and 30h of exposure. However, the intensity of activity could be arranged in a descending order: Pet.E. extract> CHCl3 extract> MeOH extract. DOI: http://dx.doi.org/10.3329/jles.v8i0.20153 J. Life Earth Sci., Vol. 8: 101-104, 2013

Bioactive Potentials of Cassia renigera Benth. Extracts against the Stored Product Pests Callosobruchus chinensis L., Sitophilus oryzae L. and Tribolium castaneum (Hbst.)

2017 ◽  
Vol 9 (4) ◽  
pp. 351-357 ◽  
Author(s):  
M. Nesa ◽  
S. Islam ◽  
S. K. Sharma ◽  
S. B. Rekha ◽  
J. Ferdous ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Sitophilus Oryzae ◽  
Test Organism ◽  
Stem Bark ◽  
Repellent Activity ◽  
Callosobruchus Chinensis ◽  
Ether Extract ◽  
Leaf Extracts ◽  
Level Of Significance ◽  
Cassia Renigera

Petroleum (Pet.) ether, CHCl3 and CH3OH extracts of Cassia renigera Benth. were tested against Callosobruchus chinensis L. for dose-mortality and repellency; and against Sitophilus oryzae L. and Tribolium castaneum (Hbst.) for repellent activity test. The Pet. ether extract of leaf was found active against C. chinensis adults through dose mortality assay and the LD50 values established were 9.270, 4.932, 2.032, 1.721, 1.671, 1.543, 1.513, 1.503 and 1.478 mg cm-2 for 0.5, 6, 12, 18, 24, 30, 36, 42 and 48 h of exposure respectively. However, the leaf extracts of CHCl3 and CH3OH; as well as the Pet. ether, CHCl3 and CH3OH extracts of the stem-bark did not give mortality to the test organism. The CHCl3 extract of leaf showed repellent activity against Tribolium castaneum at 0.1% level of significance (P < 0.001), and the Pet. ether extracts of the leaf and the CH3OH extracts of the stem-bark offered repellency at 1% level of significance (P < 0.01) between dose levels. Only the CHCl3 extracts of the stem-bark gave repellency at 5% level of significance (P < 0.05), while the CH3OH extracts of leaf and Pet. ether extract of stem-bark did not show any significant repellency at all.

scholarly journals Insecticidal activity of different fractions of petroleum ether extract of Zingiber cassumunar rhizome against Tribolium castaneum

2015 ◽  
Vol 50 (2) ◽  
pp. 143-152 ◽  
Author(s):  
D Talukder ◽  
AB MH Haque ◽  
S Zaman ◽  
MS Islam ◽  
MN Islam ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Tribolium Castaneum ◽  
Petroleum Ether Extract ◽  
Preparative Thin Layer Chromatography ◽  
Contact Toxicity ◽  
Ether Extract ◽  
Small Column ◽  
Moderate Effect ◽  
Zingiber Cassumunar ◽  
Layer Chromatography

An experiment was carried out to investigate the efficacy of contact toxicity of different fractions of petroleum ether extract of Zinziber cassumunar Roxb. rhizome against Tribolium castaneum. Seventy-eight different fractions of petroleum ether extract were obtained from column chromatography. Elutes having the similar TLC behavior were combined in eight fractions and were named as: MN-1, MN-2, MN-3, MN-4, MN-5, MN-6, MN-7 and MN-8. Four separate fractions were collected from the MN-1 fraction by preparative thin layer chromatography. These four parts were washed with petroleum ether, chloroform, ethyl acetate and methanol. Thereafter, these were separated by small column and designated as: M1N1, M1N2, M1N3, M1N4, M2N1, M2N2, M2N3, M2N4, M3N1, M3N2, M3N3, M3N4, M4N1 and M4N2. Fractions of M1N2, M2N2, M3N2 and M4N2 were found to be the most effective against the beetle T. castaneum after 24, 48 and 72 hours. However, some fractions exhibited the moderate effect and other fractions did not work against the beetle.Bangladesh J. Sci. Ind. Res. 50(2), 143-152, 2015

scholarly journals Brine Shrimp (Artemia salina Leach.) Lethality Test of Ethanolic Extract from Green Betel (Piper betle Linn.) and Red Betel (Piper crocatum Ruiz and Pav.) through the Soxhletation Method for Cytotoxicity Test

2021 ◽  
Vol 9 (A) ◽  
pp. 407-412
Author(s):  
Nerdy Nerdy ◽  
Puji Lestari ◽  
Jon Piter Sinaga ◽  
Selamat Ginting ◽  
Nilsya Febrika Zebua ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Ethanolic Extract ◽  
Artemia Salina ◽  
Lethal Concentration ◽  
Piper Betle ◽  
Cytotoxicity Test ◽  
Pharmacological Effects ◽  
Phytochemical Content ◽  
Anticancer Properties ◽  
Lc50 Value

Traditional medicine is currently being explored and developed widely by various countries because its cost effectiveness and reduced side effects. The green betel (Piper betle Linn.) and the red betel (Piper crocatum Ruiz and Pav.) are plants from the same Piper genus that are widely cultivated in Indonesia and have various phytochemical properties and pharmacological effects. The objective of this research is to determine the cytotoxicity of green leaves and red betel leaves. This research was conducted in two main stages: first, the extraction and preparation through the soxhletation method; second, a cytotoxicity test through the brine shrimp (Artemia salina Leach) lethality test (BSLT). The results showed that there were lethal concentration 50 % (LC50) value in both green betel leaves ethanolic extract and red betel leaves ethanolic extract, with 44.975 μg per mL and 31.556 μg per mL, respectively. The red betel (leaves ethanolic extract has a higher cytotoxicity than green betel (leaves ethanolic extract which can be seen from the lower lethal concentration of 50% (LC50) value. The difference in cytotoxicity is due to differences in plant varieties that cause variations in phytochemical content which affects the pharmacological effects. The green betel leaves ethanolic extract and the red betel leaves ethanolic extract are cytotoxic and have potential anticancer properties.

scholarly journals Black cumin Potential Anticancer effect of Black Cumin seed (Nigella sativa L.) extracts as determined by Cytotoxicity test against Larvae of Artemia salina Leach using Brine Shrimp Lethality Test (BSLT)

2020 ◽  
Vol 13 (2) ◽  
pp. 25-29
Author(s):  
Rasidah ◽  
Vonna Aulianshah
Keyword(s):  
Brine Shrimp ◽  
Cytotoxic Effect ◽  
Nigella Sativa ◽  
Artemia Salina ◽  
Cytotoxicity Test ◽  
Black Cumin ◽  
Black Seed ◽  
Black Cumin Seed ◽  
Seed Extracts ◽  
Cumin Seed

Black cumin plant seed or black seed (Nigella sativa L.) is among the most commonly used spices. This plant is grown mostly for its spicy seeds. Studies have reported that black cumin seeds have high levels of antioxidant that correlates with anticancer activities. The current study aims to determine the cytotoxic effect of black seed extracts on brine shrimp larvae (Artemia salina Leach) using Brine Shrimp Lethality Test (BSLT). Black cumin seed extract was obtained through percolation method using an extraction solvent (ethanol 96%). The cytotoxicity test was performed at 0 ppm concentration (control) and 10, 50, 100, 250 and 500 ppm concentrations of black seed extracts. We use 10 Brine Shrimp larvae per tube and repeated the test three times for each test group. Our results show that ethanolic extract (90%) of black cumin seeds (Nigella sativa L.) has a strong cytotoxic effect on Artemia salina larvae with the LC50 value of 107.2 ppm.

Eupatoric Acid: A Novel Triterpene from Eupatorium odoratum L. (Asteraceae)

2005 ◽  
Vol 60 (9) ◽  
pp. 1006-1011 ◽  
Author(s):  
Sajan Amatya ◽  
Sarbajna M. Tuladhar
Keyword(s):  
Palmitic Acid ◽  
Brine Shrimp ◽  
Petroleum Ether Extract ◽  
Spectroscopic Studies ◽  
Artemia Salina ◽  
The Novel ◽  
Ether Extract ◽  
Phytochemical Studies ◽  
First Time ◽  
Ethylhexyl Phthalate

Phytochemical studies on the petroleum ether extract of the roots of Eupatorium odoratum have resulted in the isolation of a novel triterpene, 3β -hydroxy-28-carboxyolean-12-ene (1) along with seven known compounds - poriferasterol (2), octadecane (3), butyrospermol acetate (4), bis(2- ethylhexyl)phthalate (5), chrysophanol (6), physcion (7) and palmitic acid (8). Novel compound 1 is designated as eupatoric acid. Compounds 2 - 7 were reported here for the first time from this plant. Palmitic acid (8) was also isolated for the first time from this root. The structure of the novel compound was established on the basis of spectroscopic studies. The cytotoxicity of the compounds 1 - 7 was studied using a lethality test against Artemia salina (brine shrimp).

scholarly journals Antimicrobial and Cytotoxic Activities of Root Extracts of Piper Chaba

2008 ◽  
Vol 1 (1) ◽  
pp. 138-144 ◽  
Author(s):  
Tarannum Naz ◽  
Ashik Mosaddik ◽  
M. Ekramul Haque
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Primary Standard ◽  
Artemia Salina ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Ether Extract ◽  
Antifungal Properties ◽  
Bacteria And Fungi ◽  
Antibacterial And Antifungal

  Antibacterial and antifungal properties of petroleum ether, chloroform, ethyl acetate and methanol extracts of Piper chaba (Choi) roots were studied by disc diffusion method and these activities were compared with primary standard drugs Kanamycin and Nystatin, respectively. The extracts were found to exhibit promising antibacterial and antifungal properties against Gram-positive, Gram-negative bacteria and fungi. The extracts were also studied for their cytotoxic activities by brine shrimp lethality bioassay, where gallic acid was used as primary standard. It was observed that the petroleum ether extract was potent cytotoxic with the LC50 value of 0.95 µg/ml against Artemia salina (L). The essential oils of the petroleum ether extract of Piper chaba roots were analysed by GC/MS. It was observed that most of the compounds were sesquiterpenes, some were long chain fatty acids and some were monoterpenes and alkaloids. Seventeen compounds were identified from the GC/MS analysis. Keywords: Piper chaba, antibacterial activity, antifungal activity, cytotoxicity. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1446

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