scholarly journals Dose-mortality, Cytotoxicity and Repellent Activity of Abutilon hirtum (Lam.) Sweet against Callosobruchus chinensis (L.), Artemia salina L. and Tribolium castaneum (Hbst.)

2017 ◽  
Vol 9 (4) ◽  
pp. 367-373
Author(s):  
S. Hossain ◽  
S. A. Rimi ◽  
H. Ali ◽  
R. A. Shawon ◽  
M. Abdullah ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Artemia Salina ◽  
Repellent Activity ◽  
Callosobruchus Chinensis ◽  
Ether Extract ◽  
Stored Grain ◽  
Cytotoxic Effects ◽  
Aerial Parts ◽  
Chcl3 Extract ◽  
Level Of Significance

Petroleum ether (Pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of the aerial parts of Abutilon hirtum (Lam.) Sweet were subjected to dose-mortality against the stored grain pest Callosobruchus chinensis (L.), cytotoxicity against brine Shrimp Artemia salina L. nauplii and repellent activity against adult beetles of Tribolium castaneum (Hbst.). Against C. chinensis only CH3OH extract showed promising mortality and provided LD50 values 1.344, 1.294, 1.243 and 1.152 mg/cm2 after 6, 12, 18 and 24 h of exposure respectively, however, Pet. ether and CHCl3 extracts didn’t show mortality. Against A. salina nauplii Pet. ether, CHCl3 and CH3OH extracts showed cytotoxic effects; while Pet. ether extract gave LC50 values 2461.031, 642, 191.233, 94.618 ppm after 6, 12, 18 and 24 h of exposure respectively, and CHCl3 extract provided LC50 values 1336.124, 679.387, 276.961 and 199.988 ppm; and CH3OH offered 531.896, 212.840, 91.499 and 72.975 ppm after 6, 12, 18 and 24 h of exposure respectively. For repellency against T. castaneum adults CHCl3 extract showed significant result at 1% level of significance (P < 0.01), while the CH3OH extract showed moderate repellency at 5% level of significance (P < 0.05), but the Pet. ether extract didn’t show any significant repellent activity.

scholarly journals Bioactive Potentials of Cleome viscosa L. Extracts: Dose-mortality, Insect Repellency and Brine Shrimp Lethality

2017 ◽  
Vol 9 (4) ◽  
pp. 375-382
Author(s):  
S. A. Rimi ◽  
S. Hossain ◽  
S. Islam ◽  
Z. Islam ◽  
S. B. Chhabi ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Tribolium Castaneum ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Callosobruchus Chinensis ◽  
Cytotoxicity Test ◽  
Ether Extract ◽  
Chcl3 Extract ◽  
Level Of Significance ◽  
Cleome Viscosa

Petroleum ether (Pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of Cleome viscosa have thoroughly screened against Tribolium castaneum (Hbst.) for repellency and dose mortality, against Callosobruchus chinensis for dose mortality activities, and against Artemia salina nauplii for lethality. In case of repellency of T. castaneum, Pet. ether extract found significantly active at 1% level of significance (P < 0.01), CHCl3 extract offered activity at 5% level of significance (P < 0.05) and CH3OH extract did not show any activity at all. In case of mortality against T. castaneum  the LD50 values of Pet. ether, CHCl3, and CH3OH extracts were 2.380, 2.188, 1.867, 1.658 and 1.429 mg/cm2; 4.950, 2.906, 2.664, 2.658 and 2.609 mg/cm2; and 4.950 and 2.791, 2.609, 2.552 and 2.474 mg/cm2 after 0.5, 6, 12, 18 and 24 h of exposure respectively.  Against C. chinensis the LD50 values of the same extracts were 0.781, 0.670, 0.642, 0.614, 0.560 and 0.494 mg/cm2; 1.964, 1.050, 0.867, 0.725, 0.635 and 0.590 mg/cm2; and 2.631, 1.851, 1.471, 1.703, 1.524 and 1.457 mg/cm2 after  6, 12, 18, 24, 30 and 36 h of exposure respectively. In case of cytotoxicity test against A. salina, the extracts offered LC50 values in a descending order CH3OH > CHCl3 > Pet. ether extracts.

scholarly journals Phytochemical Action of White Mulberry Extracts against Three Important Stored Grain Pests

2017 ◽  
Vol 9 (4) ◽  
pp. 359-366
Author(s):  
Z. R. Sabuj ◽  
A. Khatun ◽  
M. Abdullah ◽  
D. M. Badruddoza ◽  
H. Ali ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Tribolium Castaneum ◽  
Sitophilus Oryzae ◽  
Stem Bark ◽  
Callosobruchus Chinensis ◽  
Ether Extract ◽  
Stored Grain ◽  
Chcl3 Extract ◽  
Stored Grain Pests ◽  
White Mulberry

Petroleum ether (Pet. ether), CHCl3 and CH3OH extracts of white mulberry were subjected to dose-mortality tests against Callosobruchus chinensis (L.), Sitophilus oryzae (L.) and Tribolium castaneum (Hbst.). Pet. ether extract of  leaves against C. chinensis, S. oryzae and T. castaneum provided LD50 9.16, 5.06, 3.79, 2.81, 2.06, 1.55, 1.32, 1.38 mg/g; 17.6, 12.9, 11.3, 82.4, 83.5, 9.47, 8.73, 8.61 mg/g and 0.93, 0.826, 0.691, 0.623, 0.567, 0.532, 0.525 mg/cm2 after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against C. chinensis Pet. ether extract of root and stem bark gave LD50 28.3, 20.7, 18.4, 16.9, 14.4, 13.6, 13.3, 13.3 mg/g and 86.3, 228.9, 177, 109, 61.0, 21.8, 0.999, 15 mg/g after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. CH3OH extracts of leaves offered LD50 21.5, 20.4, 17, 16.2, 15.6, 14.7 mg/g against C. chinensis; 5.4, 14.7, 13.7, 11, 10.9, 9.46, 9.10 mg/g against S. oryzae; and 3.804, 3.429, 3.284, 2.736, 2.441, 2.311, 2.264 mg/cm2 against T. castaneum after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against S. oryzae CH3OH extract of root and CHCl3 of stem bark gave LD50 19.5, 19.1, 17.5, 16.7, 16.2, 15.8, 15.9 mg/g and 9.77, 9.44, 7.71, 6.56, 5.94, 5.39, 5.4 mg/g after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. However, CHCl3 extract of leaves and root didn’t show mortality.

Bioactive Potentials of Cassia renigera Benth. Extracts against the Stored Product Pests Callosobruchus chinensis L., Sitophilus oryzae L. and Tribolium castaneum (Hbst.)

2017 ◽  
Vol 9 (4) ◽  
pp. 351-357 ◽  
Author(s):  
M. Nesa ◽  
S. Islam ◽  
S. K. Sharma ◽  
S. B. Rekha ◽  
J. Ferdous ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Sitophilus Oryzae ◽  
Test Organism ◽  
Stem Bark ◽  
Repellent Activity ◽  
Callosobruchus Chinensis ◽  
Ether Extract ◽  
Leaf Extracts ◽  
Level Of Significance ◽  
Cassia Renigera

Petroleum (Pet.) ether, CHCl3 and CH3OH extracts of Cassia renigera Benth. were tested against Callosobruchus chinensis L. for dose-mortality and repellency; and against Sitophilus oryzae L. and Tribolium castaneum (Hbst.) for repellent activity test. The Pet. ether extract of leaf was found active against C. chinensis adults through dose mortality assay and the LD50 values established were 9.270, 4.932, 2.032, 1.721, 1.671, 1.543, 1.513, 1.503 and 1.478 mg cm-2 for 0.5, 6, 12, 18, 24, 30, 36, 42 and 48 h of exposure respectively. However, the leaf extracts of CHCl3 and CH3OH; as well as the Pet. ether, CHCl3 and CH3OH extracts of the stem-bark did not give mortality to the test organism. The CHCl3 extract of leaf showed repellent activity against Tribolium castaneum at 0.1% level of significance (P < 0.001), and the Pet. ether extracts of the leaf and the CH3OH extracts of the stem-bark offered repellency at 1% level of significance (P < 0.01) between dose levels. Only the CHCl3 extracts of the stem-bark gave repellency at 5% level of significance (P < 0.05), while the CH3OH extracts of leaf and Pet. ether extract of stem-bark did not show any significant repellency at all.

scholarly journals Cytotoxicity Assessment of Heritiera Littoralis (Aiton), Madhuca Longifolia (König) Macbr., Nerium Indicum Mill. And Sapium Indicum (Willd.) Leaves on Artemia Salina (L.)

2021 ◽  
Vol 29 (2) ◽  
pp. 139-149
Author(s):  
N Fatema ◽  
K Hasan ◽  
A Islam ◽  
N Yasmin ◽  
M Nesa ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Artemia Salina ◽  
Ether Extract ◽  
Chcl3 Extract ◽  
Madhuca Longifolia ◽  
Cytotoxicity Assessment

Petroleum ether, CHCl3 and CH3OH extracts of leaves of Heritiera littoralis (Aiton), Madhuca longifolia (König) Macbr., Nerium indicum Mill. and Sapium indicum (Willd.) were subjected to assess cytotoxicity against Artemia salina (L.) nauplii. The petroleum ether extract of H. littoralis leaves showed LC50 values 273.77, 97.27, 51.60, 37.12, 14.60 and 12.59 ppm after 12, 18, 24, 30, 36 and 42 h; the CHCl3 extract showed LC50 values 733.25, 105.51, 40.72 and 18.20 ppm after 6, 12, 18 and 24 h whereas CH3OH extract showed 73.05, 30.62, 24.56, 20.85, 16.21 and 6.71 ppm after 6, 12, 18, 24, 30 and 36 h of exposure respectively. The petroleum ether extract of M. longifolia leaves possess LC50 values 259.35, 115.17, 56.84 and 8.73 ppm after 12, 18, 24 and 30 h; the CHCl3 extract possess LC50 values 585.43, 205.86, 112.74, 75.62, 52.84and 47.34 ppm after 12, 18, 24, 30, 36 and 42 h but CH3OH extract possess LC50 values 185.87, 60.70, 30.11 and 15.39 ppm after 12, 18, 24 and 30 h of exposure respectively. The petroleum ether extract of N. Indicum leaves recorded LC50 values 249.82, 146.07, 80.23, 54.21 and 40.19 ppm after 18, 24, 30, 36 and 42 h; the CHCl3 extract gave LC50 values 36.13, 21.72, 19.03, 16.81 and 16.34 ppm after 12, 18, 24, 30 and 36 h but CH3OH extract recorded LC50 values 394.90, 129.69, 81.50, 73.10 and 37.51 ppm after 18, 24, 30, 36 and 42 h of exposure respectively. Similarly, the petroleum ether extract of S. indicum leaves showed LC50 values 24.79, 13.18 and 4.61 ppm after 12, 18 and 24 h; the CHCl3 extract were 50.45, 42.64, 21.20 and 14.93 ppm after 18, 24, 30 and 36 h of exposure and the CH3OH extract showed LC50 values 306.37, 217.18, 149.38, 73.52, 54.45 and 22.91 ppm after 12, 18, 24, 30, 36 and 42 h of exposure respectively. The intensity of efficacy of the extracts could be arranged in the following descending order of S. indicum (petroleum etroleum ether extract) >H. littoralis (CH3OH extract) >M. longifolia (petroleum ether extract) >N. indicum (CHCl3 extract). J. Bio-Sci. 29(2): 139-149, 2021 (December)

scholarly journals Bioactivity Studies of Solanum ferox L. Against Tribolium castaneum (Herbst) Adults

2014 ◽  
Vol 7 ◽  
pp. 29-32
Author(s):  
M Abdullah ◽  
M Nesa ◽  
R Islam ◽  
J Banu ◽  
J Sarkar ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Leaf Extract ◽  
Red Flour Beetle ◽  
Repellent Activity ◽  
Root Extract ◽  
Fruit Extract ◽  
Root Extracts ◽  
Flour Beetle ◽  
Stem Extract ◽  
Level Of Significance

Dose-mortality and repellent activity tests of the chloroform extracts of fruit, leaf, root and stem of Solanum ferox L. against the red flour beetle Tribolium castaneum (Herbst) adults have been done. The fruit, leaf and stem extracts offered promising toxicity to the beetles within 30 min. of exposure. The LD50 values for 30 min., 12, 24, 36 and 48h of exposures were 0.440, 0.247, 0.211, 0.207 and 0.164mg cm-2 respectively for the fruit extract; 2.394, 1.712, 1.540, 1.239 and 0.993mg cm-2 respectively for the leaf extract; and 4.918, 0.871, 0.546, 0.472 and 0.522mg cm-2 respectively for the stem extract. However, the root extract did not offer any mortality for 30 min. and 12h of exposure; and thus the LD50 values for 24, 36 and 48h of exposure were 6.667, 5.086 and 3.357mg cm-2 respectively. While the dose-mortality activity can be arranged in the order of fruit> leaf> stem> root extracts. The fruit and stem extracts showed repellent activity against T. castaneum adults at P<0.01 and the leaf extract at P<0.05 level of significance, while the root extract did not show any repellency. DOI: http://dx.doi.org/10.3329/jles.v7i0.20118 J. Life Earth Sci., Vol. 7: 29-32, 2012

Toxic and repellent action of sugarcane bagasse-based lignin against some stored grain insect pests

1970 ◽  
Vol 25 ◽  
pp. 27-30 ◽  
Author(s):  
LA Muslima Khanam ◽  
D Talukder ◽  
MA Hye
Keyword(s):  
Tribolium Castaneum ◽  
Sugarcane Bagasse ◽  
Laboratory Experiments ◽  
Callosobruchus Maculatus ◽  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Repellent Activity ◽  
Stored Grain ◽  
Time Intervals ◽  
Class V

Laboratory experiments were carried out to investigate the efficacy of sugarcane bagasse-based lignin against four stored grain insect pests viz., Tribolium castaneum, Tribolium confusum, Sitophilus oryzae and Callosobruchus maculatus. Mortality was assessed after 24, 48 and 72 h of treatment and LD50 values were calculated at the respective time intervals. It was observed that T. castaneum is more susceptible to the lignin than the other test insects. Repellent activity of the product was conducted for T. castaneum and T. confusum. All the concentrations of the product were found to be repellent to both the insect species. Strong repellent activity was observed at concentrations of 471.57 and 628.76 µg.cm-2 to T. confusum showing the repellency of class V activity. Key words: Bagasse-based lignin, toxicity, repellency, Tribolium castaneum, T. confusum, Sitophilus oryzae, Callosobruchus maculatus Univ. j. zool. Rajshahi Univ. Vol.25 2006 p.27-30

scholarly journals Repellent activity of Abroma augusta extracts against Tribolium castaneum (Herbst) adults

2014 ◽  
Vol 20 ◽  
pp. 49-55 ◽  
Author(s):  
Omar Ali Mondal ◽  
Jahunul Haque ◽  
Esarul Haque ◽  
Ataur Rahman Khan
Keyword(s):  
Tribolium Castaneum ◽  
Filter Paper ◽  
Stem Bark ◽  
Repellent Activity ◽  
Adhesive Tape ◽  
Stored Grain ◽  
Stem Wood ◽  
Choice Chamber ◽  
Directional Stimulus ◽  
Dry Out

Context: Repellency test can play an important role in special environmental conditions. Objectives: The main objective of this study was to make a standard protocol to control the pest by screening the test materials using adult beetles by repellency test to see whether or not the extracts contain any potential to repel the stored grain pest. Materials and Methods: The experiment was conducted in choice chamber, which was divided by half filter paper discs (Whatman No. 40, diameter 9 cm). One half of the filter paper was loaded with untreated (control) and the other half was loaded with the extract of A. augusta. All the CHCl3 extracts were separately applied onto each of the half-discs and allowed to dry out in the air for 10 mins. Each treated half-disc was then attached lengthwise, edge-to-edge, to a control half-disc with adhesive tape and placed in petri dishes. The orientation of the same was changed in the replicates to avoid the effects of any external directional stimulus affecting the distribution of the test insects Tribolium castaneum (Herbst). Ten adult insects were released in the middle of each of the filter-paper circles and each test was replicated five times. Results: All the test extracts of seed, leaves, root wood, stem bark and stem wood of A. augusta collected in chloroform showed repellent activity against adult beetles of T. castaneum at dose levels of 314.540, 157.270, 78.635, 39.318, 19.659 and, 9.831 ?g/cm2 on filter paper. According to the intensity of repellency the results could be arranged in the order: stem bark >seed> root wood > leaf > stem wood and in all the cases significant differences were obtained. Conclusion: The present results revealed that A. augusta extracts can be used as a reduced risk repellent compound in the grain and cereal stores to manage the population of T. castaneum. The results also seen to be encouraging when there is a greater need for environment-friendly pesticides then ever before. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17655 J. bio-sci. 20: 49-55, 2012

scholarly journals Evaluation of Biological Activities of Evolvulus Nummularius L. through Insecticidal, Insect Repellency and Brine Shrimp Lethality Tests

2014 ◽  
Vol 8 ◽  
pp. 101-104
Author(s):  
Md Moniruzzaman ◽  
Nayeema Parvin ◽  
Sabina Sultana ◽  
Mohammad Abdullah ◽  
Ataur Rahman Khan ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Brine Shrimp ◽  
Biological Activities ◽  
Artemia Salina ◽  
Moderate Activity ◽  
Meoh Extract ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Chcl3 Extract ◽  
Brine Shrimp Lethality Assay

Assessment of Evolvulus nummularius L. through whole plant extracts (collected in petroleum ether [Pet.E.], chloroform [CHCl3] and methanol [MeOH]) were made against Tribolium castaneum (Hbst.) for insecticidal and insect repellency and against Artemia salina L. for brine shrimp lethality, and the results were promising. The LC50 values against T. castaneum adults for Pet.E. extract were 1.280, 1.220, 1.096 and 1.030?g cm-2; for CHCl3 extract 3.249, 2.990, 2.526 and 2.204?g cm-2; and for MeOH extract 2.925, 1.899, 1.875 and 1.789?g cm-2, respectively for 12, 24, 36 and 48h of exposure. The intensity of activity could be arranged in a descending order: Pet.E. extract> MeOH extract> CHCl3 extract. For repellency, CHCl3 and MeOH extracts offered moderate activity (P <0.01), but the Pet.E. extract did not show efficacy against the beetles. The same extracts responded positively in brine shrimp lethality assay where the LC50 values for Pet.E. extract were 141.553, 98.941, 24.684 and 8.560ppm; for CHCl3 extracts 699.048, 366.663, 326.0039 and 40.114ppm; and for MeOH extracts 2336.982, 1258.605, 355.962 and 137.0589ppm respectively for 12, 18, 24 and 30h of exposure. However, the intensity of activity could be arranged in a descending order: Pet.E. extract> CHCl3 extract> MeOH extract. DOI: http://dx.doi.org/10.3329/jles.v8i0.20153 J. Life Earth Sci., Vol. 8: 101-104, 2013

scholarly journals Screening of Glycosmis pentaphylla for Cytotoxic and Repellent Potentials agains Artemia salina Nauplii and Tribolium castaneum (Herbst) Adults

1970 ◽  
pp. 33-36 ◽  
Author(s):  
MS Pramanik ◽  
MY Akter ◽  
AE Ekram ◽  
H Islam ◽  
AR Khan ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Tribolium Castaneum ◽  
Stem Bark ◽  
Artemia Salina ◽  
Repellent Activity ◽  
Root Bark ◽  
Stem Wood ◽  
Plant Parts ◽  
Wood Extracts ◽  
Wood Extract

Chloroform extracts of the leaf, stem bark, stem wood and roots of Glycosmis pentaphylla (Retz.) were tested against the brine shrimp, Artemia salina nauplii for cytotoxic activity in which the dose-mortality assay revealed LC50 values of 28.579, 28.659, 57.213 and 84.111 ppm respectively, for the plant parts efficacy of which could be arranged in the order of leaf > stem bark > stem wood > root. The same extracts were tested for repellency against Tribolium castaneum (Herbst) adults where the root bark and root wood were separately extracted and used. The F values of the arcsin transformed data were 60.983, 14.177, 19.437, 15.429 and 1.082 respectively for the leaf, stem bark, stem wood, root bark and root wood extracts. Except for the root wood extract, strong repellent activity was observed for the rest of the extracts (P<0.001). Key words: Artemia salina; Tribolium castaneum; Glycosmis pentaphylla; cytotoxic activity; repellent activity. DOI: 10.3329/jles.v3i0.7443 J. Life Earth Sci., Vols. 3-4: 33-36, 2009

Bioefficacy of enhanced diatomaceous earth and botanical powders on the mortality and progeny production of Acanthoscelides obtectus (Coleoptera: Chrysomelidae), Sitophilus granarius (Coleoptera: Dryophthoridae) and Tribolium castaneum (Coleoptera: Tenebrionidae) in stored grain cereals

2017 ◽  
Vol 37 (04) ◽  
pp. 243-258 ◽  
Author(s):  
Charles Adarkwah ◽  
Daniel Obeng-Ofori ◽  
Vanessa Hörmann ◽  
Christian Ulrichs ◽  
Matthias Schöller
Keyword(s):  
Tribolium Castaneum ◽  
Diatomaceous Earth ◽  
Adult Mortality ◽  
Botanical Insecticides ◽  
Management Approach ◽  
Sitophilus Granarius ◽  
Progeny Production ◽  
Acanthoscelides Obtectus ◽  
Stored Grain ◽  
Plant Powders

Abstract Food losses caused by insects during postharvest storage are of paramount economic importance worldwide, especially in Africa. Laboratory bioassays were conducted in stored grains to determine the toxicity of powders of Eugenia aromatica and Moringa oleifera alone or combined with enhanced diatomaceous earth (Probe-A® DE, 89.0% SiO2 and 5% silica aerogel) to adult Sitophilus granarius, Tribolium castaneum and Acanthoscelides obtectus. Adult mortality was observed up to 7 days, while progeny production was recorded at 6–10 weeks. LD50 and LT50 values for adult test insects exposed to plant powders and DE, showed that A. obtectus was the most susceptible towards the botanicals (LD50 0.179% and 0.088% wt/wt for E. aromatica and M. oleifera, respectively), followed by S. granarius. Tribolium castaneum was most tolerant (LD50 1.42% wt/wt and 1.40% wt/wt for E. aromatica and M. oleifera, respectively). The combined mixture of plant powders and DE controlled the beetles faster compared to the plant powders alone. LT50 ranged from 55.7 h to 62.5 h for T. castaneum exposed to 1.0% M. oleifera and 1.0% DE, and 0.5% E. aromatica and 1.0% DE, respectively. Botanicals caused significant reduction of F1 adults compared to the control. Combined action of botanical insecticides with DE as a grain protectant in an integrated pest management approach is discussed.

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