Cytotoxicity Assessment of Heritiera Littoralis (Aiton), Madhuca Longifolia (König) Macbr., Nerium Indicum Mill. And Sapium Indicum (Willd.) Leaves on Artemia Salina (L.)

Petroleum ether, CHCl3 and CH3OH extracts of leaves of Heritiera littoralis (Aiton), Madhuca longifolia (König) Macbr., Nerium indicum Mill. and Sapium indicum (Willd.) were subjected to assess cytotoxicity against Artemia salina (L.) nauplii. The petroleum ether extract of H. littoralis leaves showed LC50 values 273.77, 97.27, 51.60, 37.12, 14.60 and 12.59 ppm after 12, 18, 24, 30, 36 and 42 h; the CHCl3 extract showed LC50 values 733.25, 105.51, 40.72 and 18.20 ppm after 6, 12, 18 and 24 h whereas CH3OH extract showed 73.05, 30.62, 24.56, 20.85, 16.21 and 6.71 ppm after 6, 12, 18, 24, 30 and 36 h of exposure respectively. The petroleum ether extract of M. longifolia leaves possess LC50 values 259.35, 115.17, 56.84 and 8.73 ppm after 12, 18, 24 and 30 h; the CHCl3 extract possess LC50 values 585.43, 205.86, 112.74, 75.62, 52.84and 47.34 ppm after 12, 18, 24, 30, 36 and 42 h but CH3OH extract possess LC50 values 185.87, 60.70, 30.11 and 15.39 ppm after 12, 18, 24 and 30 h of exposure respectively. The petroleum ether extract of N. Indicum leaves recorded LC50 values 249.82, 146.07, 80.23, 54.21 and 40.19 ppm after 18, 24, 30, 36 and 42 h; the CHCl3 extract gave LC50 values 36.13, 21.72, 19.03, 16.81 and 16.34 ppm after 12, 18, 24, 30 and 36 h but CH3OH extract recorded LC50 values 394.90, 129.69, 81.50, 73.10 and 37.51 ppm after 18, 24, 30, 36 and 42 h of exposure respectively. Similarly, the petroleum ether extract of S. indicum leaves showed LC50 values 24.79, 13.18 and 4.61 ppm after 12, 18 and 24 h; the CHCl3 extract were 50.45, 42.64, 21.20 and 14.93 ppm after 18, 24, 30 and 36 h of exposure and the CH3OH extract showed LC50 values 306.37, 217.18, 149.38, 73.52, 54.45 and 22.91 ppm after 12, 18, 24, 30, 36 and 42 h of exposure respectively. The intensity of efficacy of the extracts could be arranged in the following descending order of S. indicum (petroleum etroleum ether extract) >H. littoralis (CH3OH extract) >M. longifolia (petroleum ether extract) >N. indicum (CHCl3 extract). J. Bio-Sci. 29(2): 139-149, 2021 (December)

Sesquiterpene Lactones with the 12,8-Guaianolide Skeleton from Algerian Centaurea omphalotricha

In continuing our investigation on the chemical diversity of Algerian plants, we examined Centaurea omphalotricha, whose chemical composition has been poorly studied. The present work was aimed at characterizing the secondary metabolite pattern of the CHCl3 extract of the aerial parts of this plant that displayed antiproliferative properties in a preliminary screening on HeLa cell line. The chemical analysis led us to characterize the bioactive oxygenated terpenoid fraction which includes, within major known metabolites, two new minor sesquiterpene lactones, centaurolide-A (1) and centaurolide-B (2). The structures of two compounds exhibiting the 12,8-guaianolide skeleton were determined by spectroscopic methods as well as by chemical correlation with inuviscolide (3), a well-known bioactive guaianolide isolated from Dittrichia (=Inula) viscosa. Centaurolides A and B represent the first report of 8,12-guaianolide skeleton in Centaurea genus. The effect of new compounds 1 and 2 and inuviscolide (3) on HeLa cell has also been evaluated.

Density Functional Theory (DFT)-Aided Structure Elucidation of Linear Diterpenes from the Irish Brown Seaweed Bifurcaria bifurcata

Brown alga Bifurcaria bifurcata is an extraordinarily rich source of linear (acylic) diterpenes with enormous structural diversity. As part of our interest into secondary metabolites of the Irish seaweeds, here we report four new acyclic diterpenes (1–4) and seven known terpenoids (5–11) from the CHCl3 extract of B. bifurcata. The planar structures of the new metabolites were elucidated by means of 1D and 2D NMR, HRMS, and FT-IR spectroscopy. Since linear diterpenes are highly flexible compounds, the assignment of their stereochemistry by conventional methods, e.g., NOESY NMR, is difficult. Therefore, we employed extensive quantum-mechanical prediction of NMR chemical shifts and optical rotation analyses to identify the relative and absolute configurations of the new compounds 1–4. Several compounds moderately inhibited the human breast cancer cell line (MDA-MB-231) with IC50 values ranging from 10.0 to 33.5 μg/mL. This study not only demonstrates the vast capacity of the Irish B. bifurcata to produce highly oxygenated linear diterpenoids, but also highlights the potential of new methodologies for assignment of their stereogenic centers.

Flavanones from the rhizomes of Boesenbergia pandurata

Boesenbergia pandurata (Roxb.) Schltr. is a perennial medicinal herb belonging to the Zingiberaceae family. It is cultivated in some tropical countries in Southeast Asia including Vietnam, Thailand, Myanmar, Indonesia and Malaysia. In Vietnam, it is known as "Ngai bun", and the fresh rhizomes are mainly used as a spice. This plant contains prenylated chalcones and other flavonoids as the major bioactive constituents. From the CHCl3 extract of Boesenbergia pandurata, we had isolated six flavanones. Their structures, pinocembrin (1), pinostrobin (2), alpinetin (3), di-Omethylpinocembrin (4), naringenin (5) and 5-Omethylnaringenin (6), were determined based on extensive spectroscopic analysis and in comparison with previous works.

PHYTOCHEMICAL CONSTITUENTS, PHENOLIC CONTENTS, AND ANTIOXIDANT ACTIVITY OF CRATAEGUS AZAROLUS EXTRACTS

Objective: The aim of this study was the isolation and identification of secondary metabolites from Crataegus azarolus (L.) and the antioxidant evaluation of its extracts and compounds.Methods: The air-dried powdered parts of the plant were extracted with 70% methanol and fractionated by chloroform, ethyl acetate, and n-butanol. The n-butanol extract was separated using polyamide SC6 column and silica gel TLC. In addition, a fraction of silica gel column of the CHCl3 extract was analyzed by gas chromatography–mass spectrometer (GC–MS). The total phenolic and total flavonoid contents of CHCl3 and n-butanol extracts were estimated. Furthermore, the antioxidant activities of CHCl3, n-butanol extracts, and two flavonoids were evaluated according to five different methods.Results: Eight compounds were identified in CHCl3 and n-butanol extracts, among them, five volatile compounds identified by GC–MS for the 1st time from the species, as well as three known flavonol glycosides identified by spectral analysis (ultraviolet,1H-nuclear magnetic resonance [NMR], and13C-NMR) and by comparison with literature data. The n-butanol extract showed the higher content of polyphenols (307.33 ± 2.33 mg (gallic acid equivalents)/g extract) and flavonoids (143.0 ± 2.12 mg QE/g extract) and it proves the highest antioxidant activity with all assays used.Conclusion: Five volatile compounds were identified for the 1st time from the C. azarolus and the antioxidant potential of plant extracts was measured using five different methods.

Bioactive Potentials of Cleome viscosa L. Extracts: Dose-mortality, Insect Repellency and Brine Shrimp Lethality

Petroleum ether (Pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of Cleome viscosa have thoroughly screened against Tribolium castaneum (Hbst.) for repellency and dose mortality, against Callosobruchus chinensis for dose mortality activities, and against Artemia salina nauplii for lethality. In case of repellency of T. castaneum, Pet. ether extract found significantly active at 1% level of significance (P < 0.01), CHCl3 extract offered activity at 5% level of significance (P < 0.05) and CH3OH extract did not show any activity at all. In case of mortality against T. castaneum the LD50 values of Pet. ether, CHCl3, and CH3OH extracts were 2.380, 2.188, 1.867, 1.658 and 1.429 mg/cm2; 4.950, 2.906, 2.664, 2.658 and 2.609 mg/cm2; and 4.950 and 2.791, 2.609, 2.552 and 2.474 mg/cm2 after 0.5, 6, 12, 18 and 24 h of exposure respectively. Against C. chinensis the LD50 values of the same extracts were 0.781, 0.670, 0.642, 0.614, 0.560 and 0.494 mg/cm2; 1.964, 1.050, 0.867, 0.725, 0.635 and 0.590 mg/cm2; and 2.631, 1.851, 1.471, 1.703, 1.524 and 1.457 mg/cm2 after 6, 12, 18, 24, 30 and 36 h of exposure respectively. In case of cytotoxicity test against A. salina, the extracts offered LC50 values in a descending order CH3OH > CHCl3 > Pet. ether extracts.

Phytochemical Action of White Mulberry Extracts against Three Important Stored Grain Pests

Petroleum ether (Pet. ether), CHCl3 and CH3OH extracts of white mulberry were subjected to dose-mortality tests against Callosobruchus chinensis (L.), Sitophilus oryzae (L.) and Tribolium castaneum (Hbst.). Pet. ether extract of leaves against C. chinensis, S. oryzae and T. castaneum provided LD50 9.16, 5.06, 3.79, 2.81, 2.06, 1.55, 1.32, 1.38 mg/g; 17.6, 12.9, 11.3, 82.4, 83.5, 9.47, 8.73, 8.61 mg/g and 0.93, 0.826, 0.691, 0.623, 0.567, 0.532, 0.525 mg/cm2 after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against C. chinensis Pet. ether extract of root and stem bark gave LD50 28.3, 20.7, 18.4, 16.9, 14.4, 13.6, 13.3, 13.3 mg/g and 86.3, 228.9, 177, 109, 61.0, 21.8, 0.999, 15 mg/g after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. CH3OH extracts of leaves offered LD50 21.5, 20.4, 17, 16.2, 15.6, 14.7 mg/g against C. chinensis; 5.4, 14.7, 13.7, 11, 10.9, 9.46, 9.10 mg/g against S. oryzae; and 3.804, 3.429, 3.284, 2.736, 2.441, 2.311, 2.264 mg/cm2 against T. castaneum after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against S. oryzae CH3OH extract of root and CHCl3 of stem bark gave LD50 19.5, 19.1, 17.5, 16.7, 16.2, 15.8, 15.9 mg/g and 9.77, 9.44, 7.71, 6.56, 5.94, 5.39, 5.4 mg/g after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. However, CHCl3 extract of leaves and root didn’t show mortality.

Dose-mortality, Cytotoxicity and Repellent Activity of Abutilon hirtum (Lam.) Sweet against Callosobruchus chinensis (L.), Artemia salina L. and Tribolium castaneum (Hbst.)

Petroleum ether (Pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of the aerial parts of Abutilon hirtum (Lam.) Sweet were subjected to dose-mortality against the stored grain pest Callosobruchus chinensis (L.), cytotoxicity against brine Shrimp Artemia salina L. nauplii and repellent activity against adult beetles of Tribolium castaneum (Hbst.). Against C. chinensis only CH3OH extract showed promising mortality and provided LD50 values 1.344, 1.294, 1.243 and 1.152 mg/cm2 after 6, 12, 18 and 24 h of exposure respectively, however, Pet. ether and CHCl3 extracts didn’t show mortality. Against A. salina nauplii Pet. ether, CHCl3 and CH3OH extracts showed cytotoxic effects; while Pet. ether extract gave LC50 values 2461.031, 642, 191.233, 94.618 ppm after 6, 12, 18 and 24 h of exposure respectively, and CHCl3 extract provided LC50 values 1336.124, 679.387, 276.961 and 199.988 ppm; and CH3OH offered 531.896, 212.840, 91.499 and 72.975 ppm after 6, 12, 18 and 24 h of exposure respectively. For repellency against T. castaneum adults CHCl3 extract showed significant result at 1% level of significance (P < 0.01), while the CH3OH extract showed moderate repellency at 5% level of significance (P < 0.05), but the Pet. ether extract didn’t show any significant repellent activity.

Evaluation of Biological Activities of Evolvulus Nummularius L. through Insecticidal, Insect Repellency and Brine Shrimp Lethality Tests

Assessment of Evolvulus nummularius L. through whole plant extracts (collected in petroleum ether [Pet.E.], chloroform [CHCl3] and methanol [MeOH]) were made against Tribolium castaneum (Hbst.) for insecticidal and insect repellency and against Artemia salina L. for brine shrimp lethality, and the results were promising. The LC50 values against T. castaneum adults for Pet.E. extract were 1.280, 1.220, 1.096 and 1.030?g cm-2; for CHCl3 extract 3.249, 2.990, 2.526 and 2.204?g cm-2; and for MeOH extract 2.925, 1.899, 1.875 and 1.789?g cm-2, respectively for 12, 24, 36 and 48h of exposure. The intensity of activity could be arranged in a descending order: Pet.E. extract> MeOH extract> CHCl3 extract. For repellency, CHCl3 and MeOH extracts offered moderate activity (P <0.01), but the Pet.E. extract did not show efficacy against the beetles. The same extracts responded positively in brine shrimp lethality assay where the LC50 values for Pet.E. extract were 141.553, 98.941, 24.684 and 8.560ppm; for CHCl3 extracts 699.048, 366.663, 326.0039 and 40.114ppm; and for MeOH extracts 2336.982, 1258.605, 355.962 and 137.0589ppm respectively for 12, 18, 24 and 30h of exposure. However, the intensity of activity could be arranged in a descending order: Pet.E. extract> CHCl3 extract> MeOH extract. DOI: http://dx.doi.org/10.3329/jles.v8i0.20153 J. Life Earth Sci., Vol. 8: 101-104, 2013