scholarly journals Cinnamaldehyde and α-terpineol inhibit the growth of planktonic cultures of Candida albicans and non albicans

2021 ◽  
Vol 10 (10) ◽  
pp. e554101019027
Author(s):  
Loyse Martorano Fernandes ◽  
Mariana Cavalcanti Lacerda ◽  
Yuri Wanderley Cavalcanti ◽  
Leopoldina de Fátima Dantas de Almeida
Keyword(s):  
Natural Products ◽  
Candida Albicans ◽  
Candida Glabrata ◽  
Oral Candidiasis ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Candida Krusei ◽  
Clinical Isolates ◽  
Antifungal Effect ◽  
Reference Strains

Agents based in natural products have been investigated for the treatment of oral candidiasis. This study aims to evaluate the antifungal effect of phytoconstituent cinnamaldehyde and α-terpineol in planktonic cultures of Candida albicans, Candida glabrata, Candida krusei and clinical isolates of C. albicans. Reference strains of C. albicans (ATCC 90028 and ATCC 60193), C. glabrata (ATCC 2001), C. krusei (ATCC 34135) and four clinical isolates were used. Nistatin 100,000UI was used as a positive control.  After preparation of the inoculum (1 × 103 CFU / mL), serial microdilution technique was performed using RPMI 1640 medium. Results: in reference strains, the MIC for α-terpineol ranged from 312,5 μg / mL (C. albicans 90028) to 40 μg / mL (C. krusei); and the cinnamaldehyde ranged from 40 μg / mL (C. albicans 90028, C. albicans 60193 and C. glabrata) to 20 μg / mL (C. krusei). Whereas for clinical strains, the MIC for α-terpineol ranged from 156 μg / mL to 78 μg / mL and cinnamaldehyde ranged from 78 μg / mL to 40 μg / mL. Therefore, the cinnamaldehyde and α-terpineol present an inhibitory effect against planktonic cultures of Candida albicans and not albicans.

Comparative evaluation of six chromogenic media for presumptive yeast identification

2017 ◽  
Vol 70 (12) ◽  
pp. 1074-1078 ◽  
Author(s):  
Alessandra Vecchione ◽  
Walter Florio ◽  
Francesco Celandroni ◽  
Simona Barnini ◽  
Antonella Lupetti ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Comparative Evaluation ◽  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Candida Krusei ◽  
Yeast Identification ◽  
Discrimination Ability ◽  
Candida Lusitaniae ◽  
Reference Strains

AimsThe present study was undertaken to evaluate the discrimination ability of six chromogenic media in presumptive yeast identification.MethodsWe analysed 108 clinical isolates and reference strains belonging to eight different species: Candida albicans,Candida dubliniensis, Candida tropicalis, Candida krusei, Candida glabrata, Candida parapsilosis,Candida lusitaniae and Trichosporon mucoides.ResultsC. albicans, C. tropicalis and C. krusei could be distinguished from one another in all the tested chromogenic media, as predicted by the manufacturers. In addition, C. albicans could be distinguished from C. dubliniensis on BBL CHROMagar Candida, Kima CHROMagar Candida and Brilliance Candida, and C. parapsilosis could be identified on CHROMATIC Candida agar, CHROMOGENIC Candida agar, and Brilliance Candida agar.ConclusionsBrilliance Candida provided the widest discrimination ability, being able to discriminate five out of the seven Candida species tested. Interestingly, C. tropicalis and C. krusei could be already distinguished from each other after 24 hours of incubation.

scholarly journals In vitro kill curves of a new semisynthetic echinocandin, LY-303366, against fluconazole-sensitive and -resistant Candida species.

1997 ◽  
Vol 41 (11) ◽  
pp. 2576-2578 ◽  
Author(s):  
J A Karlowsky ◽  
G A Harding ◽  
S A Zelenitsky ◽  
D J Hoban ◽  
A Kabani ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Amphotericin B ◽  
Candida Species ◽  
Candida Glabrata ◽  
Candida Krusei ◽  
Clinical Isolates

In vitro killing by a new semisynthetic echinocandin, LY-303366, was characterized using clinical isolates of fluconazole-sensitive (Y58) and -resistant (Y180) Candida albicans as well as Candida glabrata (Y7) and Candida krusei (Y171). The 24-h kill curves for Y58 and Y180 demonstrated dose-independent killing of between 1 and 2 log10 with LY-303366 at concentrations of 0.1, 1, 10, 50, 100, and 1,000 times the MIC. Regrowth did not occur at 24 h with either C. albicans isolate at the aforementioned LY-303366 concentrations. At their MICs, LY-303366 and amphotericin B produced similar killing kinetics in cultures of Y58, Y180, Y7, and Y171, while all cultures exposed to fluconazole at its MIC demonstrated stasis or growth over 24 h.

scholarly journals RELIABILITY OF QUANTIFICATION OF CANDIDA SPECIES COLONIES USING ALTERNATIVE PLATING METHODS

2019 ◽  
Vol 5 ◽  
Author(s):  
Sarah Raquel De Annunzio ◽  
Filipe Silveira Fusco ◽  
Carolina Santezi Santezi ◽  
Bárbara Donadon Reina ◽  
Lívia Nordi Dovigo
Keyword(s):  
Candida Albicans ◽  
Correlation Coefficient ◽  
Intraclass Correlation Coefficient ◽  
Candida Tropicalis ◽  
Candida Species ◽  
Candida Glabrata ◽  
Intraclass Correlation ◽  
Candida Krusei ◽  
Reference Strains ◽  
Drop Plate

Objective: to evaluate the concordance of different plating methods for quantification of Candida species colonies. Method: standardized suspensions of reference strains (Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei) were submitted to serial dilution and plating according to methods of track-dilution (TDM), drop plate (DPM) and the conventional spread plate (SPM). Data were submitted to construction of Bland–Altman diagrams, Intraclass Correlation Coefficient (ICC) and ANOVA (⍺=5%). Results: adequate concordance between the methods (CCI >0.71) was observed, and the execution of DPM was the fastest (p<0.001). However, DPM and TDM appear to result in greater values compared to SPM, especially for C. tropicalis and C. krusei. Conclusion: C. albicans and C. glabrata can be plated with DPM and TDM, but the use of these methods for C. krusei and C. tropicalis may result in count variation.

Study of the activity of Punica granatum-mediated silver nanoparticles against Candida albicans and Candida glabrata, alone or in combination with azoles or polyenes

2019 ◽  
Vol 58 (4) ◽  
pp. 564-567
Author(s):  
José António Santos Souza ◽  
Marta M Alves ◽  
Debora Barros Barbosa ◽  
Maria Manuel Lopes ◽  
Eugénia Pinto ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Candida Species ◽  
Biofilm Formation ◽  
Candida Glabrata ◽  
Oral Candidiasis ◽  
Punica Granatum ◽  
Inhibitory Effect ◽  
Green Route ◽  
Potent Inhibitory Effect

Abstract The continuous emergence of Candida strains resistant to currently used antifungals demands the development of new alternatives that could reduce the burden of candidiasis. In this work silver nanoparticles synthesized using a green route are efficiently used, alone or in combination with fluconazole, amphotericin B or nystatine, to inhibit growth of C. albicans and C. glabrata oral clinical strains, including in strains showing resistance to fluconazole. A potent inhibitory effect over biofilm formation prompted by the two Candida species was also observed, including in mature biofilm cells. These results foster the use of phytotherapeutics as effective treatments in oral candidiasis.

scholarly journals Antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis

2017 ◽  
Vol 78 (2) ◽  
pp. 368-374 ◽  
Author(s):  
I. J. Dias ◽  
E. R. I. S. Trajano ◽  
R. D. Castro ◽  
G. L. S. Ferreira ◽  
H. C. M. Medeiros ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Candida Tropicalis ◽  
Oral Candidiasis ◽  
Positive Control ◽  
Fungal Species ◽  
Candida Krusei ◽  
Candida Spp ◽  
Minimum Fungicidal Concentration

Abstract This study analyzed the antifungal activity of phytoconstituents from linalool on Candida spp. strains, in vitro, isolated from patients with clinical diagnoses of oral candidiasis associated with the use of a dental prosthesis. Biological samples were collected from 12 patients using complete dentures or removable partial dentures and who presented mucous with diffuse erythematous or stippled features, indicating a clinical diagnosis of candidiasis. To identify fungal colonies of the genus Candida, samples were plated onto CHROMagar Candida®. The antifungal activity of linalool, a monoterpene unsaturated constituent of basil oil, was performed using the broth microdilution technique. Then, the minimum inhibitory concentration (MIC), the two subsequent stronger concentrations and the positive controls were subcultured on Sabouraud Dextrose Agar plates to determine the minimum fungicidal concentration (MFC). The experiments were performed in triplicate and nystatin was used as a positive control in all tests. Diagnoses of oral candidiasis were verified in eight patients (66.6%) and the most prevalent fungal species was Candida albicans (37.5%), followed by Candida krusei (25.0%); and Candida tropicalis (4.2%). The best antifungal activity of linalool was observed on Candida tropicalis (MIC = 500 mg/mL), followed by Candida albicans (MIC = 1.000 mg/mL), and Candida krusei (MIC = 2.000 mg/mL).Under the study conditions and based on the results obtained, it can be concluded that the Candida strains tested were susceptible to linalool.

scholarly journals Determination of Minimum Inhibitory and Fungicidal Concentrations of Potash Alum Against Clinical Isolates of Candida albicans

2020 ◽  
Vol 29 (04) ◽  
pp. 235-238
Author(s):  
Muhammad Irshad ◽  
◽  
Muhamamd Younas ◽  
Asif Ullah Qureshi ◽  
Amir Hameed
Keyword(s):  
Candida Albicans ◽  
Oral Candidiasis ◽  
Opportunistic Pathogen ◽  
Positive Control ◽  
Clinical Isolates ◽  
In Vitro Susceptibility ◽  
Fungicidal Action ◽  
Broth Microdilution Method

OBJECTIVE: Candida albicans is an opportunistic pathogen causing oral candidiasis. Commercially available antifungal agents are effective in eliminating C. albicans, however, their toxicity and high cost are undesirable. Potash Alum is a naturally occurring salt with antibacterial and antifungal properties. Therefore, Potash Alum may be effective against C. albicans. Objective: The main objective of this study was to investigate the in vitro susceptibility of C. albicans to Potash Alum. METHODOLOGY: Swab samples from 19 patients attending the Oral medicine department of Rehman College of Dentistry were transferred to tubes containing Sabouraud Dextrose Broth. After identification of C. albicans by Gram-staining, a solution of 2-5 x 105 CFUs/mL C. albicans was prepared and subjected to MIC and MFC determination by the standard broth microdilution method. Potash alum concentrations of 5, 10 and 20 mg/mL were used. Commercially available Nystatin was used as a positive control. RESULTS: Our results showed that 10 mg/mL of Potash Alum (PA) solution was able to inhibit growth of most of the clinical isolates of C. albicans. In 5 samples, even 5mg/mL was effective in inhibiting the growth of C. albicans. Potash alum demonstrated fungistatic rather than a fungicidal action against C. albicans. CONCLUSIONS: It is concluded that potash alum has a fungistatic action against C. albicans in vitro. In addition, the optimum in vitro concentration of potash alum solution effective in inhibiting growth of C. albicans was found to be 10mg/mL. KEYWORDS: Candida albicans, potash alum, nystatin, antifungal HOW TO CITE: Irshad M, Younas M, Qureshi AU, Hameed A. Determination of minimum inhibitory and fungicidal concentrations of potash alum against clinical isolates of candida albicans. J Pak Dent Assoc 2020;29(4):235-238.

scholarly journals Azasordarins: Susceptibility of Fluconazole-Susceptible and Fluconazole-Resistant Clinical Isolates ofCandida spp. to GW 471558

2001 ◽  
Vol 45 (6) ◽  
pp. 1905-1907 ◽  
Author(s):  
Manuel Cuenca-Estrella ◽  
Emilia Mellado ◽  
Teresa M. Dı́az-Guerra ◽  
Araceli Monzón ◽  
Juan L. Rodrı́guez-Tudela
Keyword(s):  
Candida Albicans ◽  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Candida Guilliermondii ◽  
Candida Krusei ◽  
Clinical Isolates ◽  
In Vitro Activity ◽  
Fluconazole Resistant ◽  
Candida Lusitaniae

ABSTRACT The in vitro activity of the azasordarin GW 471558 was compared with those of amphotericin B, flucytosine, itraconazole, and ketoconazole against 177 clinical isolates of Candidaspp. GW 471558 showed potent activity against Candida albicans, Candida glabrata, and Candida tropicalis, even against isolates with decreased susceptibility to azoles. Candida krusei, Candida parapsilosis, Candida lusitaniae, and Candida guilliermondii are resistant to GW 471558 in vitro (MICs, >128 μg/ml).

scholarly journals Synthesis, Characterization and in Vitro Antifungal Effect of Some Butyltin(IV) N-substituted 2-aminoethanethiolates

2001 ◽  
Vol 8 (3) ◽  
pp. 165-169 ◽  
Author(s):  
A. Smicka ◽  
V. Buchta ◽  
K. Handlir
Keyword(s):  
Candida Glabrata ◽  
Pathogenic Fungi ◽  
Trichophyton Mentagrophytes ◽  
Candida Krusei ◽  
Antifungal Drugs ◽  
Antifungal Effect ◽  
Absidia Corymbifera ◽  
Structure Activity ◽  
Trichosporon Beigelii

Six new N-substituted di- and tributyltin 2-aminoethanethiolates (cysteaminates) have been prepared and characterised by H1, C13 and S119n NMR spectroscopy. All these compounds exhibit a good in vitro antifungal effect against selected types of human pathogenic fungi (Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata, Trichosporon beigelii, Aspergillus fumigatus, Absidia corymbifera, Trichophyton mentagrophytes) and their activity is comparable with that of some antifungal drugs commonly used in the clinical use like ketoconazole. The structure-activity relationships in these compounds are discussed.

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