Antifungal effect of high- and low-molecular-weight chitosan hydrochloride, carboxymethyl chitosan, chitosan oligosaccharide and N-acetyl-d-glucosamine against Candida albicans, Candida krusei and Candida glabrata

2007 ◽  
Author(s):  
F SEYFARTH ◽  
S SCHLIEMANN ◽  
P ELSNER ◽  
U HIPLER
Keyword(s):  
Molecular Weight ◽  
Candida Albicans ◽  
Candida Glabrata ◽  
Carboxymethyl Chitosan ◽  
Candida Krusei ◽  
Low Molecular Weight ◽  
Chitosan Oligosaccharide ◽  
Antifungal Effect ◽  
Chitosan Hydrochloride ◽  
Low Molecular Weight Chitosan

scholarly journals Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain–Chitosan Nanoparticles

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1681 ◽  
Author(s):  
Janaína Artem Ataide ◽  
Eloah Favero Gérios ◽  
Letícia Caramori Cefali ◽  
Ana Rita Fernandes ◽  
Maria do Céu Teixeira ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Physicochemical Properties ◽  
Enzyme Stability ◽  
Proteolytic Enzymes ◽  
Chitosan Nanoparticles ◽  
Low Molecular Weight ◽  
Chitosan Oligosaccharide ◽  
Nanoparticle Suspension ◽  
Accelerated Stability ◽  
Low Molecular Weight Chitosan

Bromelain, a set of proteolytic enzymes potential pharmaceutical applications, was encapsulated in chitosan nanoparticles to enhance enzyme stability, and the effect of different chitosan sources was evaluated. Chitosan types (i.e., low molecular weight chitosan, chitosan oligosaccharide lactate, and chitosan from shrimp shells) produced nanoparticles with different physicochemical properties, however in all cases, particle size and zeta potential decreased, and polydispersity index increased after bromelain addition. Bromelain encapsulation was higher than 84% and 79% for protein content and enzymatic activity, respectively, with low molecular weight chitosan presenting the highest encapsulation efficiency. Nanoparticle suspension was also tested for accelerated stability and rheological behavior. For the chitosan–bromelain nanoparticles, an instability index below 0.3 was recorded and, in general, the loading of bromelain in chitosan nanoparticles decreased the cohesiveness of the final suspension.

scholarly journals Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria

2012 ◽  
Vol 41 (5) ◽  
pp. 312-317 ◽  
Author(s):  
Rubens Spin-Neto ◽  
Felipe Leite Coletti ◽  
Rubens Moreno de Freitas ◽  
Chaíne Pavone ◽  
Sérgio Paulo Campana-Filho ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bone Density ◽  
High Molecular Weight ◽  
Critical Size ◽  
Bone Defects ◽  
Control Group ◽  
Low Molecular Weight ◽  
Chitosan Hydrochloride ◽  
Low Molecular Weight Chitosan ◽  
High Molecular Weight Chitosan

OBJECTIVE: This study evaluated, using digital radiographic images, the action of chitosan and chitosan hydrochloride biomaterials, with both low and high molecular weight, used in the correction of critical-size bone defects (CSBD's) in rat's calvaria. MATERIAL AND METHOD: CSBD's with 8 mm in diameter were surgically created in the calvaria of 50 Holtzman rats and these were filled with a blood clot (Control), low molecular weight chitosan, high molecular weight chitosan, low molecular weight chitosan hydrochloride and high molecular weight chitosan hydrochloride, for a total of 10 animals, which were divided into two experimental periods (15 and 60 days), for each biomaterial. The radiographic evaluation was made using two digital radiographs of the animal's skull: one taken right after the bone defect was created and the other at the moment of the sacrifice, providing the initial and the final radiographic bone density in the area of the defect, which were compared. RESULT: Analysis of radiographic bone density indicated that the increase in the radiographic bone density of the CSBD's treated with the proposed biomaterials, in either molecular weight, in both observed periods, where similar to those found in control group. CONCLUSION: Tested chitosan-based biomaterials were not able to enhance the radiographic density in the CSBD's made in rat's calvaria.

scholarly journals Cinnamaldehyde and α-terpineol inhibit the growth of planktonic cultures of Candida albicans and non albicans

2021 ◽  
Vol 10 (10) ◽  
pp. e554101019027
Author(s):  
Loyse Martorano Fernandes ◽  
Mariana Cavalcanti Lacerda ◽  
Yuri Wanderley Cavalcanti ◽  
Leopoldina de Fátima Dantas de Almeida
Keyword(s):  
Natural Products ◽  
Candida Albicans ◽  
Candida Glabrata ◽  
Oral Candidiasis ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Candida Krusei ◽  
Clinical Isolates ◽  
Antifungal Effect ◽  
Reference Strains

Agents based in natural products have been investigated for the treatment of oral candidiasis. This study aims to evaluate the antifungal effect of phytoconstituent cinnamaldehyde and α-terpineol in planktonic cultures of Candida albicans, Candida glabrata, Candida krusei and clinical isolates of C. albicans. Reference strains of C. albicans (ATCC 90028 and ATCC 60193), C. glabrata (ATCC 2001), C. krusei (ATCC 34135) and four clinical isolates were used. Nistatin 100,000UI was used as a positive control.  After preparation of the inoculum (1 × 103 CFU / mL), serial microdilution technique was performed using RPMI 1640 medium. Results: in reference strains, the MIC for α-terpineol ranged from 312,5 μg / mL (C. albicans 90028) to 40 μg / mL (C. krusei); and the cinnamaldehyde ranged from 40 μg / mL (C. albicans 90028, C. albicans 60193 and C. glabrata) to 20 μg / mL (C. krusei). Whereas for clinical strains, the MIC for α-terpineol ranged from 156 μg / mL to 78 μg / mL and cinnamaldehyde ranged from 78 μg / mL to 40 μg / mL. Therefore, the cinnamaldehyde and α-terpineol present an inhibitory effect against planktonic cultures of Candida albicans and not albicans.

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