scholarly journals IN-SILICO ANALYSIS OF TERPENOIDS IN SACCHAROMYCES CEREVISIAE

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Sudheer Menon
Keyword(s):  
Saccharomyces Cerevisiae ◽  
In Silico ◽  
Carbon Sources ◽  
In Silico Analysis ◽  
Mode Analysis ◽  
The Novel ◽  
Knock Out ◽  
Elementary Mode Analysis ◽  
Engineering Strategy ◽  
Host Metabolism

Heterologous production of terpenoids from plants which are of medicinal and industrial interests is getting much attention. For this purpose saccharomyces cerevisiae is the most commonly used host but the yield of terpenoids is much lower. The main aim of this review is to study the terpenoid pathways of saccharomyces cerevisiae, effect of respective host metabolism as well as to study the effect of different carbon sources in silico by means of elementary mode analysis. The production and yield of IPP was main focus point in order to find out the novel metabolic engineering strategy for increasing production of terpenoids. With glucose acting as a substrate, MVA pathway has low potential to produce terpenoids as compared to DXP pathway if we consider formation of precursor. Moreover the carbon source also has impact on yield with nonfermentable source providing more biomass. At last several knock out methodologies were being employed which identified minimal cuts sets for enhanced growth of terpenoids.

A novel missense mutation in the FGB gene (p.Gly302Arg) leading to afibrinogenemia

2016 ◽  
Vol 36 (S 02) ◽  
pp. S34-S37 ◽  
Author(s):  
H. Rühl ◽  
G. Detarsio ◽  
A. Biswas ◽  
S. Gupta ◽  
M. Davoli ◽  
...  
Keyword(s):  
Missense Mutation ◽  
In Silico ◽  
In Silico Analysis ◽  
Missense Mutations ◽  
The Novel ◽  
Beta Chain ◽  
The Core ◽  
And Function ◽  
Flanking Regions ◽  
Positively Charged

SummaryAfibrinogenemia represents the rarest form of fibrinogen deficiency. Causative missense mutations occur rarely and may improve the understanding of fibrinogen structure and function. Patients and methods: The propositus was a 26-year-old Argentinian with afibrinogenemia. FGA, FGB and FGG exons and flanking regions were screened by sequencing and the mutant protein was analyzed in silico. Results: A novel missense mutation in the FGB gene (Bbeta Gly272Arg, p.Gly302Arg) was identified. In silico analysis revealed its location in a highly conserved region, which preserves the core fold of the C-terminal beta-chain and is important for proper secretion. A substitution by a positively charged large Arg residue in this area would most likely disturb the core fold by additional interactions with adjacent residues (p.Asp291, p.Asp297, p.Asp311), or by forming of non-native interactions with other proteins, thereby hindering the action of molecular chaperones. Both alternatives would disturb the regular secretion of the beta-chain. Conclusions: The novel mis-sense mutation in the FGB gene causes afibrinogenemia most probably by affecting the secretion of the fibrinogen beta-chain.

scholarly journals In-silico analysis of heat shock protein 47 for identifying the novel therapeutic agents in the management of oral submucous fibrosis

2014 ◽  
Vol 25 (5) ◽  
pp. 580 ◽  
Author(s):  
JayasankarP Pillai ◽  
GirishJ Parmar ◽  
Rakesh Rawal ◽  
GirishR Chauhan ◽  
RajarajeswariJ Pillai ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
In Silico ◽  
Oral Submucous Fibrosis ◽  
In Silico Analysis ◽  
Therapeutic Agents ◽  
The Novel ◽  
Submucous Fibrosis ◽  
Silico Analysis ◽  
Novel Therapeutic

scholarly journals Sea Urchin Pigments as Potential Therapeutic Agents Against the Spike Protein of SARS-CoV-2 Based on in Silico Analysis

2020 ◽  
Author(s):  
Elena Susana Barbieri ◽  
Tamara Rubilar ◽  
Ayelén Gázquez ◽  
Marisa Avaro ◽  
Erina Noé Seiler ◽  
...  
Keyword(s):  
Sea Urchin ◽  
In Silico ◽  
In Silico Analysis ◽  
Protein S ◽  
Host Cells ◽  
Spike Protein ◽  
Therapeutic Drug ◽  
The Novel ◽  
Novel Coronavirus ◽  
Silico Analysis

Several studies have been published regarding the interaction between the spike protein of the novel coronavirus SARS-CoV-2 and ACE2 receptor in the host cells. In the presente work, we evaluated the in silico properties of two sea urchin pigments, Echinochrome A (EchA) and Spinochromes (SpinA) against the Spike protein (S) towards finding a potential therapeutic drug against the disease caused by the novel coronavirus (COVID-19). The best ensemble docking pose of EchaA and SpinA showed a binding affinity of -5.9 and -6.7 kcal mol-1, respectively. The linked aminoacids (T505, G496 and Y449 for EchA and Y449, Q493 and G496 for SpinA) are in positions involved in ACE2 binding in both RBDs frim SARS-CoV and SARS-CoV-2 suggesting that EchA and SpinA may interact with Spike proteins drom both viruses. The results suggest that these pigments could act as inhibitors of S protein, pointing them as antiviral drugs for SARS-CoV-2.<br>

scholarly journals Sea Urchin Pigments as Potential Therapeutic Agents Against the Spike Protein of SARS-CoV-2 Based on in Silico Analysis

2020 ◽  
Author(s):  
Elena Susana Barbieri ◽  
Tamara Rubilar ◽  
Ayelén Gázquez ◽  
Marisa Avaro ◽  
Erina Noé Seiler ◽  
...  
Keyword(s):  
Sea Urchin ◽  
In Silico ◽  
In Silico Analysis ◽  
Protein S ◽  
Host Cells ◽  
Spike Protein ◽  
Therapeutic Drug ◽  
The Novel ◽  
Novel Coronavirus ◽  
Silico Analysis

Several studies have been published regarding the interaction between the spike protein of the novel coronavirus SARS-CoV-2 and ACE2 receptor in the host cells. In the presente work, we evaluated the in silico properties of two sea urchin pigments, Echinochrome A (EchA) and Spinochromes (SpinA) against the Spike protein (S) towards finding a potential therapeutic drug against the disease caused by the novel coronavirus (COVID-19). The best ensemble docking pose of EchaA and SpinA showed a binding affinity of -5.9 and -6.7 kcal mol-1, respectively. The linked aminoacids (T505, G496 and Y449 for EchA and Y449, Q493 and G496 for SpinA) are in positions involved in ACE2 binding in both RBDs frim SARS-CoV and SARS-CoV-2 suggesting that EchA and SpinA may interact with Spike proteins drom both viruses. The results suggest that these pigments could act as inhibitors of S protein, pointing them as antiviral drugs for SARS-CoV-2.<br>

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