scholarly journals Laboratory-performance Study of the Quantitative Detection Method for Genetically Modified Soybeans (Roundup Ready Soybeans 40-3-2)

2005 ◽  
Vol 46 (6) ◽  
pp. 270-276 ◽  
Author(s):  
Kikuko KASAMA ◽  
Takahiro WATANABE ◽  
Tatsuya SUZUKI ◽  
Hiroyuki KIKUCHI ◽  
Shoko TOKISHITA ◽  
...  
Keyword(s):  
Detection Method ◽  
Genetically Modified ◽  
Quantitative Detection ◽  
Performance Study ◽  
Roundup Ready ◽  
Laboratory Performance ◽  
Genetically Modified Soybeans

scholarly journals A Detection Method for Recombinant DNA from Genetically Modified Soybeans and Processed Foods Containing Them

1999 ◽  
Vol 40 (2) ◽  
pp. 149-157_1 ◽  
Author(s):  
Takeshi MATSUOKA ◽  
Yoshimi KAWASHIMA ◽  
Hiroshi AKIYAMA ◽  
Hirohito MIURA ◽  
Yukihiro GODA ◽  
...  
Keyword(s):  
Recombinant Dna ◽  
Detection Method ◽  
Genetically Modified ◽  
Processed Foods ◽  
Genetically Modified Soybeans

scholarly journals Real-Time Detection of Genetically Modified Soya Using Lightcycler and ABI 7700 Platforms with TaqMan, Scorpion, and SYBR Green I Chemistries

2002 ◽  
Vol 85 (4) ◽  
pp. 938-944 ◽  
Author(s):  
Catherine F Terry ◽  
Della J Shanahan ◽  
Lydia D Ballam ◽  
Neil Harris ◽  
David G McDowell ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Sequences ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Sybr Green ◽  
Sybr Green I ◽  
Quantitative Detection ◽  
Roundup Ready ◽  
Detection Systems ◽  
Copy Numbers

Abstract A comparative cross platform evaluation of real-time polymerase chain reaction detection of DNA sequences present in Roundup Ready soya was undertaken using the ABI 7700 and Roche Lightcycler detection systems in combination with 3 different detection chemistries: TaqMan, Scorpion primers, and SYBR Green I fluorescent dye. Various copy numbers of a plasmid containing the soya lectin sequence were used to determine the sensitivity and reproducibility of the different technology combinations and to examine both inter and intra machine variability. To examine the relative accuracy of each technology, the genetically modified soya content of baked products containing known amounts of Roundup Ready soya was determined by detection of lectin and the EPSPS transgene. It was determined that the combination of TaqMan detection chemistry and the ABI 7700 platform represented the best method for quantitative detection of genetically modified organisms in terms of both precision and accuracy.

scholarly journals Laboratory-performance Study of the Notified Methods to Detect Genetically Modified Papaya (55-1)

2005 ◽  
Vol 46 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Hiroyuki KIKUCHI ◽  
Takahiro WATANABE ◽  
Kikuko KASAMA ◽  
Chiseko WAKUI ◽  
Akihiko MATSUKI ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Performance Study ◽  
Laboratory Performance
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