scholarly journals Effect of Native American Bean-corn Biculture Planting on Free-living Bacterial Abundance and Plant Growth

2015 ◽  
Vol 12 (4) ◽  
Author(s):  
Heather Miller ◽  
Justin Fiene ◽  
Tamara Marsh
Keyword(s):  
Native American ◽  
Plant Growth ◽  
Plant Biomass ◽  
Plant Growth Promoting Bacteria ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Soil Microbial ◽  
Plant Growth Promoting ◽  
Native American Tribes

Native American tribes with bountiful harvests. Today it is widely recognized that this associated intercropping system derives much of its success from symbiotic bacteria (e.g. Rhizobium). These bacteria colonize the roots of leguminous plants, allowing them to fix atmospheric nitrogen into ammonia. However, the effect of this intercropping practice on the microbial community, independent of the effect of the symbiotic nitrogen-fixing bacteria, is not well understood. Therefore, a study was designed to model the effects of simultaneously intercropping bean and corn on the abundance of aerobic heterotrophic, free-living nitrogen-fixing, and symbiotic nitrogen-fixing bacteria, as well as plant growth and fecundity markers. In parallel, the benefits mediated by rhizobia were evaluated by inoculating a duplicate set of treatments with N-Dure, a rhizobia-containing inoculum. Native American varieties of pole-bean (Phaseolus vulgaris L.) and corn (Zea mays mays L.) were planted in monoculture and biculture treatments. All cultivations were maintained under greenhouse conditions for 52 days with daily watering and no additional fertilizer or microbial amendments. Although a significant increase in weight per plant was noted for the inoculated biculture when compared to either the inoculated bean or corn monocultures (p ≤ 0.05), the abundance of heterotrophic and free-living nitrogen-fixing bacteria did not show a significant change from the related controls, with or without inoculation. However, symbiotic nitrogen-fixing bacteria, as measured by root nodulation, increased significantly (p ≤ 0.05) for the inoculated biculture and single planting. Thus, these data confirm that corn benefited from this associated intercropping system as shown by an increase in plant biomass that can be attributed to Rhizobium. However, neither the legume-bacteria symbiotic relationship nor the increase in plant biodiversity resulting from this intercropping practice appears to have had significant effects on the abundance of the two common soil-associated bacterial groups evaluated, though further research would be necessary to fully assess the changes to heterotrophic bacterial diversity at the species level. KEYWORDS: Three Sisters; Nitrogen-fixing Bacteria; Inoculation with Rhizobia; Plant Growth Promoting Bacteria; Soil Microbial Biota; Corn and Bean Simultaneous Planting.

scholarly journals Genomic characterization and computational phenotyping of nitrogen-fixing bacteria isolated from Colombian sugarcane fields

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luz K. Medina-Cordoba ◽  
Aroon T. Chande ◽  
Lavanya Rishishwar ◽  
Leonard W. Mayer ◽  
Lina C. Valderrama-Aguirre ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Phosphate Solubilization ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Promising Tool ◽  
A Genome ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPrevious studies have shown the sugarcane microbiome harbors diverse plant growth promoting microorganisms, including nitrogen-fixing bacteria (diazotrophs), which can serve as biofertilizers. The genomes of 22 diazotrophs from Colombian sugarcane fields were sequenced to investigate potential biofertilizers. A genome-enabled computational phenotyping approach was developed to prioritize sugarcane associated diazotrophs according to their potential as biofertilizers. This method selects isolates that have potential for nitrogen fixation and other plant growth promoting (PGP) phenotypes while showing low risk for virulence and antibiotic resistance. Intact nitrogenase (nif) genes and operons were found in 18 of the isolates. Isolates also encode phosphate solubilization and siderophore production operons, and other PGP genes. The majority of sugarcane isolates showed uniformly low predicted virulence and antibiotic resistance compared to clinical isolates. Six strains with the highest overall genotype scores were experimentally evaluated for nitrogen fixation, phosphate solubilization, and the production of siderophores, gibberellic acid, and indole acetic acid. Results from the biochemical assays were consistent and validated computational phenotype predictions. A genotypic and phenotypic threshold was observed that separated strains by their potential for PGP versus predicted pathogenicity. Our results indicate that computational phenotyping is a promising tool for the assessment of bacteria detected in agricultural ecosystems.

Nitrogen-fixing bacteria with multiple plant growth-promoting activities enhance growth of tomato and red pepper

2013 ◽  
Vol 53 (12) ◽  
pp. 1004-1015 ◽  
Author(s):  
Md. Rashedul Islam ◽  
Tahera Sultana ◽  
M. Melvin Joe ◽  
Woojong Yim ◽  
Jang-Cheon Cho ◽  
...  
Keyword(s):  
Plant Growth ◽  
Red Pepper ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Rhizobacteria Isolated from Saline Soil Induce Systemic Tolerance in Wheat (Triticum aestivum L.) against Salinity Stress

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 989 ◽  
Author(s):  
Noshin Ilyas ◽  
Roomina Mazhar ◽  
Humaira Yasmin ◽  
Wajiha Khan ◽  
Sumera Iqbal ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Salinity Stress ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Soluble Sugar ◽  
Wheat Crop ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

Halo-tolerant plant growth-promoting rhizobacteria (PGPR) have the inherent potential to cope up with salinity. Thus, they can be used as an effective strategy in enhancing the productivity of saline agro-systems. In this study, a total of 50 isolates were screened from the rhizospheric soil of plants growing in the salt range of Pakistan. Out of these, four isolates were selected based on their salinity tolerance and plant growth promotion characters. These isolates (SR1. SR2, SR3, and SR4) were identified as Bacillus sp. (KF719179), Azospirillum brasilense (KJ194586), Azospirillum lipoferum (KJ434039), and Pseudomonas stutzeri (KJ685889) by 16S rDNA gene sequence analysis. In vitro, these strains, in alone and in a consortium, showed better production of compatible solute and phytohormones, including indole acetic acid (IAA), gibberellic acid (GA), cytokinin (CK), and abscisic acid (ABA), in culture conditions under salt stress. When tested for inoculation, the consortium of all four strains showed the best results in terms of improved plant biomass and relative water content. Consortium-inoculated wheat plants showed tolerance by reduced electrolyte leakage and increased production of chlorophyll a, b, and total chlorophyll, and osmolytes, including soluble sugar, proline, amino acids, and antioxidant enzymes (superoxide dismutase, catalase, peroxidase), upon exposure to salinity stress (150 mM NaCl). In conclusion, plant growth-promoting bacteria, isolated from salt-affected regions, have strong potential to mitigate the deleterious effects of salt stress in wheat crop, when inoculated. Therefore, this consortium can be used as potent inoculants for wheat crop under prevailing stress conditions.

Impact of nitrogen fixing and plant growth-promoting bacteria on a phloem-feeding soybean herbivore

2015 ◽  
Vol 86 ◽  
pp. 71-81 ◽  
Author(s):  
S.M. Brunner ◽  
R.J. Goos ◽  
S.J. Swenson ◽  
S.P. Foster ◽  
B.G. Schatz ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Bacteria ◽  
Nitrogen Fixing ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phloem Feeding

scholarly journals Genomic characterization and computational phenotyping of nitrogen-fixing bacteria isolated from Colombian sugarcane fields

2019 ◽  
Author(s):  
Luz K. Medina-Cordoba ◽  
Aroon T. Chande ◽  
Lavanya Rishishwar ◽  
Leonard W. Mayer ◽  
Lina C. Valderrama-Aguirre ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Phosphate Solubilization ◽  
Bacterial Isolates ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
A Genome ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACTPrevious studies have shown that the sugarcane microbiome harbors diverse plant growth promoting (PGP) microorganisms, including nitrogen-fixing bacteria, and the objective of this study was to design a genome-enabled approach to prioritize sugarcane associated nitrogen-fixing bacteria according to their potential as biofertilizers. Using a systematic high throughput approach, 22 pure cultures of nitrogen-fixing bacteria were isolated and tested for diazotrophic potential by PCR amplification of nitrogenase (nifH) genes, common molecular markers for nitrogen fixation capacity. Genome sequencing confirmed the presence of intact nitrogenase nifH genes and operons in the genomes of 18 of the isolates. Isolate genomes also encoded operons for phosphate solubilization, siderophore production operons, and other PGP phenotypes. Klebsiella pneumoniae strains comprised 14 of the 22 nitrogen-fixing isolates, and four others were members of closely related genera to Klebsiella. A computational phenotyping approach was developed to rapidly screen for strains that have high potential for nitrogen fixation and other PGP phenotypes while showing low risk for virulence and antibiotic resistance. The majority of sugarcane isolates were below a genotypic and phenotypic threshold, showing uniformly low predicted virulence and antibiotic resistance compared to clinical isolates. Six prioritized strains were experimentally evaluated for PGP phenotypes: nitrogen fixation, phosphate solubilization, and the production of siderophores, gibberellic acid and indole acetic acid. Results from the biochemical assays were consistent with the computational phenotype predictions for these isolates. Our results indicate that computational phenotyping is a promising tool for the assessment of benefits and risks associated with bacteria commonly detected in agricultural ecosystems.IMPORTANCEA genome-enabled approach was developed for the prioritization of native bacterial isolates with the potential to serve as biofertilizers for sugarcane fields in Colombia’s Cauca Valley. The approach is based on computational phenotyping, which entails predictions related to traits of interest based on bioinformatic analysis of whole genome sequences. Bioinformatic predictions of the presence of plant growth promoting traits were validated with experimental assays and more extensive genome comparisons, thereby demonstrating the utility of computational phenotyping for assessing the benefits and risks posed by bacterial isolates that can be used as biofertilizers. The quantitative approach to computational phenotyping developed here for the discovery of biofertilizers has the potential for use with a broad range of applications in environmental and industrial microbiology, food safety, water quality, and antibiotic resistance studies.

scholarly journals Enhanced Plant Performance in Cicer arietinum L. due to the Addition of Combination of Plant Growth Promoting Bacteria

2017 ◽  
Author(s):  
María Pérez-Fernández ◽  
Valentine Alexander
Keyword(s):  
Plant Growth ◽  
Cicer Arietinum ◽  
Plant Biomass ◽  
Nitrogen Concentration ◽  
Plant Performance ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Promoting Effect ◽  
Plant Growth Promoting ◽  
Growth Promoting

Six bacterial strains with differing abilities to produce varying concentrations of Indole Acetic Acid were tested individually and in consortia for plant growth promoting and fitness related traits of Cicer arietinum. In all experiments the presence of the nitrogen fixer Mesorhizobium ciceri resulted in increased biomass production.  In the absence of this strain, IAA Psedomonas putida and Bacillus megaterium hinder plant growth and fitness related traits. The application of mixes of the three strains always resulted in better plant performance when M. ciceri was present. Whereas P. putida has a noticeable plant growth-promoting effect B. megaterium resulted less effective. The low levels of IAA produced by the selected strains had a significantly greater positive effect on plant biomass accumulation, flower, pods and seed production as well as on total plant nitrogen and nitrogen concentration in seed than high IAA producer strains.

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