The use of transgenic canola (Brassica napus) and plant growth-promoting bacteria to enhance plant biomass at a nickel-contaminated field site

2006 ◽  
Vol 288 (1-2) ◽  
pp. 309-318 ◽  
Author(s):  
Andrea J. Farwell ◽  
Susanne Vesely ◽  
Vincent Nero ◽  
Hilda Rodriguez ◽  
Saleh Shah ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Plant Biomass ◽  
Plant Growth Promoting Bacteria ◽  
Field Site ◽  
Transgenic Canola ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Contaminated Field

scholarly journals Bacillus pumilus increases boron uptake and inhibits rapeseed growth under boron supply irrespective of phosphorus fertilization

AoB Plants ◽  
2019 ◽  
Vol 11 (4) ◽  
Author(s):  
Sajid Masood ◽  
Xue Qiang Zhao ◽  
Ren Fang Shen
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Bacillus Pumilus ◽  
Phosphorus Fertilization ◽  
Plant Growth Promoting Bacteria ◽  
Boron Uptake ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
B Uptake ◽  
Pot Culture

AbstractThe present study was carried out to investigate how plant growth-promoting bacteria (PGPB) influence plant growth and uptake of boron (B) and phosphorus (P) in rapeseed (Brassica napus). Rapeseed was subjected to control, B, P and B + P treatments, either with or without B. pumilus (PGPB) inoculation, and grown in pot culture for 6 weeks. In the absence of B. pumilus, the addition of B, P or both elements improved the growth of rapeseed compared with the control. Interestingly, B. pumilus inoculation inhibited plant growth and enhanced B uptake under B and B + P but not under control and P conditions. In addition, B. pumilus inoculation decreased the pH of soil under B and B + P supplies. Bacillus pumilus inoculation thus increased rapeseed B uptake and inhibited growth under B supply, which suggests that the effects of PGPB on rapeseed growth depend on the addition of B to soil. Bacillus pumilus inoculation may therefore be recommended for the enhancement of rapeseed B levels in B-deficient soils but not in B-sufficient ones.

Growth of canola (Brassica napus) in the presence of plant growth-promoting bacteria and either copper or polycyclic aromatic hydrocarbons

2005 ◽  
Vol 51 (12) ◽  
pp. 1061-1069 ◽  
Author(s):  
M L.E Reed ◽  
Bernard R Glick
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Brassica Napus ◽  
Plant Growth ◽  
Aromatic Hydrocarbons ◽  
Contaminated Soils ◽  
Plant Growth Promoting Bacteria ◽  
Polycyclic Aromatic ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Copper Contaminated

Growth of canola (Brassica napus) seeds treated with plant growth-promoting bacteria in copper-contaminated and polycyclic aromatic hydrocarbon (PAH)-contaminated soils was monitored. Pseudomonas asplenii AC, isolated from PAH-contaminated soil, was transformed to express a bacterial gene encoding 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and both native and transformed bacteria were tested for growth promotion. Inoculation of seeds, grown in the presence of copper or creosote, with either native or transformed P. asplenii AC significantly increased root and shoot biomass. Native and transformed P. asplenii AC and transformed P. asplenii AC encapsulated in alginate were equally effective at promoting plant growth in copper-contaminated soils. In creosote-contaminated soils the native bacterium was the least effective, and the transformed encapsulated bacterium was the most effective in growth promotion.Key words: plant growth-promoting bacteria, phytoremediation, copper, polycyclic aromatic hydrocarbons, Brassica napus, ethylene, alginate encapsulation.

scholarly journals Rhizobacteria Isolated from Saline Soil Induce Systemic Tolerance in Wheat (Triticum aestivum L.) against Salinity Stress

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 989 ◽  
Author(s):  
Noshin Ilyas ◽  
Roomina Mazhar ◽  
Humaira Yasmin ◽  
Wajiha Khan ◽  
Sumera Iqbal ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Salinity Stress ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Soluble Sugar ◽  
Wheat Crop ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

Halo-tolerant plant growth-promoting rhizobacteria (PGPR) have the inherent potential to cope up with salinity. Thus, they can be used as an effective strategy in enhancing the productivity of saline agro-systems. In this study, a total of 50 isolates were screened from the rhizospheric soil of plants growing in the salt range of Pakistan. Out of these, four isolates were selected based on their salinity tolerance and plant growth promotion characters. These isolates (SR1. SR2, SR3, and SR4) were identified as Bacillus sp. (KF719179), Azospirillum brasilense (KJ194586), Azospirillum lipoferum (KJ434039), and Pseudomonas stutzeri (KJ685889) by 16S rDNA gene sequence analysis. In vitro, these strains, in alone and in a consortium, showed better production of compatible solute and phytohormones, including indole acetic acid (IAA), gibberellic acid (GA), cytokinin (CK), and abscisic acid (ABA), in culture conditions under salt stress. When tested for inoculation, the consortium of all four strains showed the best results in terms of improved plant biomass and relative water content. Consortium-inoculated wheat plants showed tolerance by reduced electrolyte leakage and increased production of chlorophyll a, b, and total chlorophyll, and osmolytes, including soluble sugar, proline, amino acids, and antioxidant enzymes (superoxide dismutase, catalase, peroxidase), upon exposure to salinity stress (150 mM NaCl). In conclusion, plant growth-promoting bacteria, isolated from salt-affected regions, have strong potential to mitigate the deleterious effects of salt stress in wheat crop, when inoculated. Therefore, this consortium can be used as potent inoculants for wheat crop under prevailing stress conditions.

scholarly journals Effects of Bacterial ACC Deaminase on Brassica napus Gene Expression

2012 ◽  
Vol 25 (5) ◽  
pp. 668-676 ◽  
Author(s):  
Jennifer C. Stearns ◽  
Owen Z. Woody ◽  
Brendan J. McConkey ◽  
Bernard R. Glick
Keyword(s):  
Stress Response ◽  
Brassica Napus ◽  
Plant Growth ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Hormone Regulation ◽  
Auxin Response ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plants in association with plant growth-promoting rhizobacteria can benefit from lower plant ethylene levels through the action of the bacterial enzyme 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. This enzyme cleaves the immediate biosynthetic precursor of ethylene, ACC. Ethylene is responsible for many aspects of plant growth and development but, under stressful conditions, it exacerbates stress symptoms. The ACC deaminase-containing bacterium Pseudomonas putida UW4 is a potent plant growth-promoting strain and, as such, was used to elaborate the detailed role of bacterial ACC deaminase in Brassica napus (canola) plant growth promotion. Transcriptional changes in bacterially treated canola plants were investigated with the use of an Arabidopsis thaliana oligonucleotide microarray. A heterologous approach was necessary because there are few tools available at present to measure global expression changes in nonmodel organisms, specifically with the sensitivity of microarrays. The results indicate that the transcription of genes involved in plant hormone regulation, secondary metabolism, and stress response was altered in plants by the presence of the bacterium, whereas the upregulation of genes for auxin response factors and the downregulation of stress response genes was observed only in the presence of bacterial ACC deaminase. These results support the suggestion that there is a direct link between ethylene and the auxin response, which has been suggested from physiological studies, and provide more evidence for the stress-reducing benefits of ACC deaminase-expressing plant growth-promoting bacteria.

scholarly journals Enhanced Plant Performance in Cicer arietinum L. due to the Addition of Combination of Plant Growth Promoting Bacteria

2017 ◽  
Author(s):  
María Pérez-Fernández ◽  
Valentine Alexander
Keyword(s):  
Plant Growth ◽  
Cicer Arietinum ◽  
Plant Biomass ◽  
Nitrogen Concentration ◽  
Plant Performance ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Promoting Effect ◽  
Plant Growth Promoting ◽  
Growth Promoting

Six bacterial strains with differing abilities to produce varying concentrations of Indole Acetic Acid were tested individually and in consortia for plant growth promoting and fitness related traits of Cicer arietinum. In all experiments the presence of the nitrogen fixer Mesorhizobium ciceri resulted in increased biomass production.  In the absence of this strain, IAA Psedomonas putida and Bacillus megaterium hinder plant growth and fitness related traits. The application of mixes of the three strains always resulted in better plant performance when M. ciceri was present. Whereas P. putida has a noticeable plant growth-promoting effect B. megaterium resulted less effective. The low levels of IAA produced by the selected strains had a significantly greater positive effect on plant biomass accumulation, flower, pods and seed production as well as on total plant nitrogen and nitrogen concentration in seed than high IAA producer strains.

scholarly journals Global RNA sequencing reveals enhanced photosynthetic activity and auxin response in Brassica napus treated with Pseudomonas chlororaphis PA23

2020 ◽  
Author(s):  
Mark F. Belmonte ◽  
Teresa R de Kievit ◽  
Joey C Wan ◽  
Ayo Bolaji ◽  
Emma Gray ◽  
...  
Keyword(s):  
Gene Expression ◽  
Brassica Napus ◽  
Plant Growth ◽  
Rna Sequencing ◽  
Plant Growth Promoting Bacteria ◽  
Pseudomonas Chlororaphis ◽  
Phenotypic Differences ◽  
Auxin Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth promoting bacteria (PGPB) are a growing subset of agricultural adjuncts which can be used to increase crop yield and plant productivity. Although substantial research has been conducted on the metabolites and active molecules secreted by PGPBs, relatively little is known about their effects on the global transcriptome of the host plant. The present study was carried out to investigate changes in the gene expression landscape of early vegetative Brassica napus following treatment with Pseudomonas chlororaphis PA23. This PGPB was isolated from the soybean rhizosphere and has been extensively studied as a biocontrol agent. However, little is known about its effects on plant growth and development. Using a combination of RNA-sequencing and physiological analyses, we identified increased abundance of mRNA transcripts associated with photosynthesis and phytohormone response. Phenotypically we observed increased photosynthetic rates and larger root and shoot systems in B. napus following P. chlororaphis PA23 treatment. Lastly, we identified auxin production by P. chlororaphis PA23 which likely contributes to changes in gene expression and the observed phenotypic differences in root and shoot structures. Together, the results of our study suggest that PA23 is a potent plant growth promoting agent with the potential for field applications as an agricultural adjunct.

Isolation and identification of plant growth-promoting bacteria associated with tall fescue

2009 ◽  
pp. 211-216 ◽  
Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan
Keyword(s):  
New Zealand ◽  
Plant Growth ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Growth Promoting Bacteria ◽  
Beneficial Bacteria ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

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