DETECTION OF BACILLUS THURINGIENSIS CRYSTAL PROTEIN CRY51AA1 INTOXICATION (IN VIVO) USING ZEBRAFISH MODEL

Pancreatic β-cell loss is critical in diabetes pathogenesis. Up to now, no effective treatment has become available for β-cell loss. A polyphenol recently isolated from Polysiphonia japonica, 5-Bromoprotocatechualdehyde (BPCA), is considered as a potential compound for the protection of β-cells. In this study, we examined palmitate (PA)-induced lipotoxicity in Ins-1 cells to test the protective effects of BPCA on insulin-secreting β-cells. Our results demonstrated that BPCA can protect β-cells from PA-induced lipotoxicity by reducing cellular damage, preventing reactive oxygen species (ROS) overproduction, and enhancing glucose-stimulated insulin secretion (GSIS). BPCA also improved mitochondrial morphology by preserving parkin protein expression. Moreover, BPCA exhibited a protective effect against PA-induced β-cell dysfunction in vivo in a zebrafish model. Our results provide strong evidence that BPCA could be a potential therapeutic agent for the management of diabetes.

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Development of BCR-ABL1 Transgenic Zebrafish Model Reproducing Chronic Myeloid Leukemia (CML) Like-Disease and Providing a New Insight into CML Mechanisms

Cells ◽

10.3390/cells10020445 ◽

2021 ◽

Vol 10 (2) ◽

pp. 445

Author(s):

Daniela Zizioli ◽

Simona Bernardi ◽

Marco Varinelli ◽

Mirko Farina ◽

Luca Mignani ◽

...

Keyword(s):

Chronic Myeloid Leukemia ◽

Myeloid Leukemia ◽

Fusion Gene ◽

Philadelphia Chromosome ◽

Leukemic Cells ◽

Transgenic Zebrafish ◽

Hematopoietic Tissue ◽

Zebrafish Model ◽

Altered Expression

Zebrafish has proven to be a versatile and reliable experimental in vivo tool to study human hematopoiesis and model hematological malignancies. Transgenic technologies enable the generation of specific leukemia types by the expression of human oncogenes under specific promoters. Using this technology, a variety of myeloid and lymphoid malignancies zebrafish models have been described. Chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasia characterized by the BCR-ABL1 fusion gene, derived from the t (9;22) translocation causing the Philadelphia Chromosome (Ph). The BCR-ABL1 protein is a constitutively activated tyrosine kinas inducing the leukemogenesis and resulting in an accumulation of immature leukemic cells into bone marrow and peripheral blood. To model Ph+ CML, a transgenic zebrafish line expressing the human BCR-ABL1 was generated by the Gal4/UAS system, and then crossed with the hsp70-Gal4 transgenic line. The new line named (BCR-ABL1pUAS:CFP/hsp70-Gal4), presented altered expression of hematopoietic markers during embryonic development compared to controls and transgenic larvae showed proliferating hematopoietic cells in the caudal hematopoietic tissue (CHT). The present transgenic zebrafish would be a robust CML model and a high-throughput drug screening tool.

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Anti-seizure activity of African medicinal plants: the identification of bioactive alkaloids from the stem bark of Rauvolfia caffra using an in vivo zebrafish model

Journal of Ethnopharmacology ◽

10.1016/j.jep.2021.114282 ◽

2021 ◽

pp. 114282

Author(s):

Talent Chipiti ◽

Alvaro M. Viljoen ◽

Maria L. Cordero-Maldonado ◽

Clinton.G.L. Veale ◽

Fanie R. Van Heerden ◽

...

Keyword(s):

Medicinal Plants ◽

Seizure Activity ◽

Stem Bark ◽

Zebrafish Model

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In vivo SAR and STR analyses of alkaloids from Picrasma quassioides identify 1-hydroxymethyl-8-hydroxy-β-carboline as a novel natural angiogenesis inhibitor

RSC Advances ◽

10.1039/c5ra22391a ◽

2016 ◽

Vol 6 (12) ◽

pp. 9484-9494 ◽

Cited By ~ 12

Author(s):

Guiyi Gong ◽

Qinghua Lin ◽

Jian Xu ◽

Feng Ye ◽

Lingling Jiang ◽

...

Keyword(s):

Angiogenesis Inhibitor ◽

Zebrafish Model ◽

Picrasma Quassioides

Twenty alkaloids were obtained from the anti-angiogenic fraction of Picrasma quassioides and their SAR/STR were studies by a zebrafish model. We had identified 3 as an angiogenesis inhibitor and confirmed in vitro.

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