scholarly journals Laboratory Evaluation of the Immune Response of Young Broiler Chickens Vaccinated Against Newcastle Disease Under Field Conditions

1981 ◽  
Vol 60 (6) ◽  
pp. 1204-1208 ◽  
Author(s):  
J.J. GIAMBRONE
2010 ◽  
Vol 7 (2) ◽  
pp. 296-302 ◽  
Author(s):  
MA Jalil ◽  
MA Samad ◽  
MT Islam

The study was conducted to determine the persistence of maternally derived antibody (MDA) and its effects on protection against NDV in broiler chickens and to investigate the status of humoral immune response following vaccination with BCRDV® (F-strain, lentogenic) at different ages of broiler chickens during the period from August to October,  2008. A total of 90 day-old broiler chicks of Cobb 500 strain with the history of vaccination of parent stock against Newcastle disease (ND) was divided into three groups (A, B and C). Birds of group A (n = 35) were used for the study of protection ability of MDA against NDV, the birds of group B (n = 45) were used for the measurement of humoral immune response in chickens following vaccination at different ages and birds of group C (n = 10) were used for the determination of persistence of maternally derived antibody. The level of antibody titre against NDV was determined by HI test. The protective potentiality of MDA and vaccine was determined by the rate of survivability of the chickens following challenge infection. It was observed that the MDA titre in day-old chicks was higher and gradually declined at minimal level at day 28. The MDA titre of 128 or above protected the birds following challenge infection with virulent NDV. There were significant decrease in HI titres of chickens which were vaccinated once at day 1 and day 7, and could not withstand challenge infection with virulent NDV. Single vaccination with BCRDV® at day 14 triggered the production of antibody but could not provide complete protection to the birds. The birds which were boosted with the same vaccine 7 days and 21 days after primary vaccination produced better immune response. However, the birds which were vaccinated primarily at day 1 and boosted at day 7 could not withstand the challenge completely. Of the other regimens of twice vaccination, primary vaccination at day 7 and booster dosing at day 28 was found to be the best in terms of immune response and protection potentiality. Therefore, it may be concluded that (a) The MDA titre level of 128 or above is sufficient to protect broilers against challenge with virulent NDV,( b) Primary vaccination at day 7 followed by a booster dosing at day 28 may be followed for better immune response and protection against ND in broilers.DOI: 10.3329/bjvm.v7i2.5995Bangl. J. Vet. Med. (2009). 7(2) : 296 – 302


2020 ◽  
Vol 99 (6) ◽  
pp. 2944-2954
Author(s):  
Nahed A. El-Shall ◽  
Ramadan S. Shewita ◽  
Mohamed E. Abd El-Hack ◽  
Abdullah AlKahtane ◽  
Saud Alarifi ◽  
...  

2019 ◽  
Vol 12 (8) ◽  
pp. 1251-1255 ◽  
Author(s):  
Gaichamdinliu Gonmei ◽  
Deben Sapcota ◽  
Girin Kumar Saikia ◽  
Pankaj Deka ◽  
Joga Dev Mahanta ◽  
...  

Background and Aim: The chicken gut harbors microflora which impacts the health, production performance and immune response against pathogens. Assam local chickens reared under natural conditions are known to possess high immunocompetence which may be attributable to its gut microbiota make-up. This study aimed to investigate the individual effect of two strains of Lactobacillus reuteri PIA16 isolated separately from cecum and jejunum of Assam indigenous chicken on the immunity of broiler chickens against Newcastle disease virus (NDV) when fed singly and in combination with a prebiotic. Materials and Methods: A total of 240 birds (48 per group) were vaccinated with Lasota strain of NDV on the 5th and 21st day of age. Blood samples were collected before and after immunization against ND for the detection of humoral antibody response by hemagglutination inhibition test. The cell-mediated immune (CMI) response was estimated through response to phytohemagglutinin-P (PHA-P) and expressed as web index. Results: A significant influence on the immune response to NDV was observed in all the L. reuteri PIA16 as well as mannan oligosaccharide (MOS) supplemented groups revealing higher antibody titer than the control counterpart. The CMI response revealed a better cutaneous basophilic hypersensitivity response to PHA-P in the treated groups than the control. Conclusion: Enhancement in immunity was perceived in the broilers fed with L. reuteri PIA16 and in combination with MOS due to the stimulation of the host's humoral and CMI response by the probiotics and prebiotics used. Keywords: hemagglutination inhibition, Lactobacillus reuteri PIA16, mannan oligosaccharide, Newcastle disease, phytohemagglutinin-P, probiotics.


Científica ◽  
2018 ◽  
Vol 46 (2) ◽  
pp. 194
Author(s):  
Paulo Cesar Riquelme Salazar ◽  
Gustavo Do Valle Polycarpo ◽  
Julio Cezar Dadalt ◽  
Pedro De Assunção Pimenta Ribeiro ◽  
Maria Fernanda De Castro Burbarelli ◽  
...  

2016 ◽  
Vol 10 (3) ◽  
pp. 134-140
Author(s):  
M.M. Zaki ◽  
Wafaa A. Abd El-Ghany ◽  
Maha M. Hady ◽  
Reda M.S. Korany

1970 ◽  
Vol 6 (2) ◽  
pp. 139-144
Author(s):  
MS Islam ◽  
AKM Khasruzzaman ◽  
MT Hossain ◽  
MT Islam ◽  
MH Chowdhury ◽  
...  

A study was undertaken to determine the immune response of eight different imported live NDV vaccines in broiler chickens in the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh during the period from July to December 2008. A total of 55 broiler chickens (Ross breed) were divided into eleven groups such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 of which group 1, 3, 5, 7 and 9 were vaccinated primarily with Nobilis® MA5+Clone30, Avipro® ND-IB HB1, Cevac® BIL, Newcastle-Bronchitis Vaccine Fortdodge® and Avipro® ND LaSota vaccine respectively at day 5 of age and secondarily with Nobilis® ND Clone 30, Avipro® ND LaSota, Cevac® New L, Newcastle Disease vaccine Fortdodge® and Avipro® ND LaSota vaccine respectively at day 21 of age by single eye instillation and 2, 4, 6, 8 and 10 were vaccinated with the same vaccines respectively by double eye instillation following the same schedule. Group 11 was kept as unvaccinated control. Sera samples were collected after 10 days of each vaccination and at day 5, 15, 20, 31 of age from nonvaccinated control and subjected to HI test for the determination of antibody titres. It was observed that after primary vaccination the geometric mean (GM) of HI titres of double eye vaccinated groups differed significantly (P


2016 ◽  
Vol 4 (4) ◽  
pp. 33-36
Author(s):  
Forough Talazadeh ◽  
Mansoor Mayahi ◽  
katayoon nofouzi ◽  
Ebrahim Golzari ◽  
Rahil Chegini

2014 ◽  
Vol 38 (1) ◽  
pp. 30-39
Author(s):  
Nidhal Raoof Mahdi

The main objectives of this study was to determine the influence of soluble β-glucan extracted from the cell wall of Saccharomyces cerevisiae on immune response of broiler chickens reared under heat stress .β-glucan 225μg/ml was supplemented in drinking water to broiler chicken vaccinated with Newcastle disease virus (NDV) vaccine. The parameters of the assessment of the immune response was the Heterophil / Lymphocyte ratio as a measure of stress, determination of the serum antibody titer post vaccination with the NDV vaccine by ELISA test and Immunohistochemical detection of macrophages by using monoclonal antibodies (mouse antichicken macrophage KUL01). A hundred and twenty (120) Chickens one day old were divided into two equal groups; group under heat stress and group control; each group was divided into two subgroups (G1, G2, G3 and G4) containing thirty chicks. The experiment was conducted for six weeks. The stressed group exposed to heat stress (≃35ºC) starting from the third week of age upto the end of the experiment. While (Group 1) and (G3) chicks were supplemented with 225µg/ml of soluble β-glucan in drinking water from day 1 to the end of the experiment, while (G2 and G4) chicks were not supplemented. The result of Heterophil /Lymphocyte ratio indicates that there was a significant (P < 0.05) difference within heat stressed treated which showed an elevated H/L ratio at 21,28 and 35 days old. Also there was a significant (P < 0.05) difference between groups that were treated with β-glucan (G1, G3) at 21, 28 and 32 days of age compared with a control non treated non stressed group (G4) at same periods. The results of antibody titer to NDV showed that there were significant (P < 0.05) differences among all groups at 7, 14 and 21 days of age, and the results of immunohistochemical analysis demonstrated positive staining for duodenal and bursal macrophages labeled with KUL-01 mouse anti-chicken monocyte- macrophages monoclonal antibodies. Tissue sections of duodenum and bursa at 14 and 24 days old stressed and non-stressed groups treated with β-glucan showed a positive result (purple-brown staining macrophages) in G1 and G3 as compared with duodenal and bursal tissues of G2 and G4groups which showed no stained cells.


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