scholarly journals Proteomic analysis reveals changes in carbohydrate and protein metabolism associated with broiler breast myopathy

2017 ◽  
Vol 96 (8) ◽  
pp. 2992-2999 ◽  
Author(s):  
Vivek A. Kuttappan ◽  
Walter Bottje ◽  
Ranjith Ramnathan ◽  
Steven D. Hartson ◽  
Craig N. Coon ◽  
...  
Keyword(s):  
Protein Metabolism ◽  
Proteomic Analysis ◽  
Broiler Breast

Proteomic Analysis Identifies Dysfunction in Cellular Transport, Energy, and Protein Metabolism in Different Brain Regions of Atypical Frontotemporal Lobar Degeneration

2012 ◽  
Vol 11 (4) ◽  
pp. 2533-2543 ◽  
Author(s):  
Daniel Martins-de-Souza ◽  
Paul C. Guest ◽  
David M. Mann ◽  
Sigrun Roeber ◽  
Hassan Rahmoune ◽  
...  
Keyword(s):  
Protein Metabolism ◽  
Proteomic Analysis ◽  
Frontotemporal Lobar Degeneration ◽  
Brain Regions ◽  
Cellular Transport

scholarly journals Comparative Proteomic Analysis of Coregulation of CIPK14 and WHIRLY1/3 Mediated Pale Yellowing of Leaves in Arabidopsis

2018 ◽  
Vol 19 (8) ◽  
pp. 2231 ◽  
Author(s):  
Zhe Guan ◽  
Wanzhen Wang ◽  
Xingle Yu ◽  
Wenfang Lin ◽  
Ying Miao
Keyword(s):  
Protein Metabolism ◽  
Proteomic Analysis ◽  
Photochemical Efficiency ◽  
Time Of Flight ◽  
Photochemical Quenching ◽  
Relative Distribution ◽  
Ribulose Bisphosphate Carboxylase ◽  
Ribulose Bisphosphate ◽  
Ps Ii ◽  
Bisphosphate Carboxylase

Pale yellowing of leaf variegation is observed in the mutant Arabidopsis lines Calcineurin B-Like-Interacting Protein Kinase14 (CIPK14) overexpression (oeCIPK14) and double-knockout WHIRLY1/WHIRLY3 (why1/3). Further, the relative distribution of WHIRLY1 (WHY1) protein between plastids and the nucleus is affected by the phosphorylation of WHY1 by CIPK14. To elucidate the coregulation of CIPK14 and WHIRLY1/WHIRLY3-mediated pale yellowing of leaves, a differential proteomic analysis was conducted between the oeCIPK14 variegated (oeCIPK14-var) line, why1/3 variegated (why1/3-var) line, and wild type (WT). More than 800 protein spots were resolved on each gel, and 67 differentially abundant proteins (DAPs) were identified by matrix-assisted laser desorption ionization-time of flight/time of flight mass spectrometry (MALDI-TOF/TOF-MS). Of these 67 proteins, 34 DAPs were in the oeCIPK14-var line and 33 DAPs were in the why1/3-var line compared to the WT. Five overlapping proteins were differentially expressed in both the oeCIPK14-var and why1/3-var lines: ATP-dependent Clp protease proteolytic subunit-related protein 3 (ClpR3), Ribulose bisphosphate carboxylase large chain (RBCL), Beta-amylase 3 (BAM3), Ribosome-recycling factor (RRF), and Ribulose bisphosphate carboxylase small chain (RBCS). Bioinformatics analysis showed that most of the DAPs are involved in photosynthesis, defense and antioxidation pathways, protein metabolism, amino acid metabolism, energy metabolism, malate biosynthesis, lipid metabolism, and transcription. Thus, in the why1/3-var and oeCIPK14-var lines, there was a decrease in the photosystem parameters, including the content of chlorophyll, the photochemical efficiency of photosystem (PS II) (Fv/Fm), and electron transport rates (ETRs), but there was an increase in non-photochemical quenching (NPQ). Both mutants showed high sensitivity to intense light. Based on the annotation of the DAPs from both why1/3-var and oeCIPK14-var lines, we conclude that the CIPK14 phosphorylation-mediated WHY1 deficiency in plastids is related to the impairment of protein metabolism, leading to chloroplast dysfunction.

scholarly journals Proteomic analysis of the second-generation merozoites of Eimeria tenella under nitromezuril and ethanamizuril stress

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Xue-Yan Li ◽  
Li-Li Liu ◽  
Min Zhang ◽  
Li-Fang Zhang ◽  
Xiao-Yang Wang ◽  
...  
Keyword(s):  
Treatment Group ◽  
Drug Treatment ◽  
Protein Metabolism ◽  
Proteomic Analysis ◽  
Second Generation ◽  
Protein Composition ◽  
Eimeria Tenella ◽  
Label Free ◽  
Altered Expression ◽  
Ppi Networks

Abstract Background Eimeria tenella is a highly pathogenic coccidian that causes avian coccidiosis. Both nitromezuril (NZL) and ethanamizuril (EZL) are novel triazine compounds with high anticoccidial activity, but the mechanisms of their action are still unclear. This study explored the response of E. tenella to NZL and EZL by the study of changes in protein composition of the second-generation merozoites. Methods Label-free quantification (LFQ) proteomics of the second-generation merozoites of E. tenella following NZL and EZL treatment were studied by LC-MS/MS to explore the mechanisms of action. The identified proteins were annotated and analyzed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and protein-protein interaction (PPI) networks analysis. Results A total of 1430 proteins were identified by LC-MS/MS, of which 375 were considered as differential proteins in response to drug treatment (DPs). There were 26 only found in the NZL treatment group (N-group), 63 exclusive to the EZL treatment group (E-group), and 80 proteins were present in both drug groups. In addition, among the DPs, the abundant proteins with significantly altered expression in response to drug treatment (SDPs) were found compared with the C-group, of which 49 were upregulated and 51 were downregulated in the N-group, and 66 upregulated and 79 downregulated in the E-group. Many upregulated proteins after drug treatment were involved in transcription and protein metabolism, and surface antigen proteins (SAGs) were among the largest proportion of the downregulated SDPs. Results showed the top two enriched GO terms and the top one enriched pathway treated with EZL and NZL were related, which indicated that these two compounds had similar modes of action. Conclusions LFQ proteomic analysis is a feasible method for screening drug-related proteins. Drug treatment affected transcription and protein metabolism, and SAGs were also affected significantly. This study provided new insights into the effects of triazine anticoccidials against E. tenella.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Proteomic Analysis Helps Identify Mycosis Fungoides

2007 ◽  
Vol 38 (8) ◽  
pp. 13
Author(s):  
SHERRY BOSCHERT
Keyword(s):  
Mycosis Fungoides ◽  
Proteomic Analysis

Proteomic analysis of the anti-obesity effect of Taeumjowui-Tang in high fat diet-induced obese mice

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
E Changkyun Park ◽  
SY Lee ◽  
SH Yun ◽  
WY Kim ◽  
Y Yi ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
High Fat Diet ◽  
Obese Mice ◽  
High Fat

Proteomic analysis of clear cell foci in the human liver identifies differential expression of Starch-Binding Domain-Containing Protein 1 (STBD1)

2019 ◽  
Author(s):  
C Metzendorf ◽  
K Wineberger ◽  
A Cigliano ◽  
DF Calvisi ◽  
F Dombrowski ◽  
...  
Keyword(s):  
Differential Expression ◽  
Proteomic Analysis ◽  
Human Liver ◽  
Clear Cell ◽  
Binding Domain

EARLY EFFECTS OF GLUCOCORTICOIDS ON RIBONUCLEIC ACID AND PROTEIN METABOLISM IN RAT LIVER

1963 ◽  
Vol 44 (4_Suppla) ◽  
pp. S3-S36 ◽  
Author(s):  
Kristian F. Jervell
Keyword(s):  
Rat Liver ◽  
Ribonucleic Acid ◽  
Protein Metabolism ◽  
Early Effects
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