scholarly journals Gene Expression Signatures of Synovial Fluid Multipotent Stromal Cells in Advanced Knee Osteoarthritis and Following Knee Joint Distraction

2020 ◽  
Vol 8 ◽  
Author(s):  
Clara Sanjurjo-Rodriguez ◽  
Ala Altaie ◽  
Simon Mastbergen ◽  
Thomas Baboolal ◽  
Tim Welting ◽  
...  
Keyword(s):  
Gene Expression ◽  
Synovial Fluid ◽  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Joint Distraction ◽  
Gene Expression Signatures

scholarly journals Effects of exercise therapy on knee joint function and synovial fluid cytokine levels in patients with knee osteoarthritis

2012 ◽  
Vol 7 (1) ◽  
pp. 183-186 ◽  
Author(s):  
SHAO-LAN ZHANG ◽  
HONG-QI LIU ◽  
XIAO-ZU XU ◽  
JUAN ZHI ◽  
JIAO-JIAO GENG ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Exercise Therapy ◽  
Joint Function ◽  
Cytokine Levels

scholarly journals Knee joint distraction in regular care for treatment of knee osteoarthritis: A comparison with clinical trial data

PLoS ONE ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. e0227975 ◽  
Author(s):  
Mylène P. Jansen ◽  
Simon C. Mastbergen ◽  
Ronald J. van Heerwaarden ◽  
Sander Spruijt ◽  
Michelle D. van Empelen ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Clinical Trial Data ◽  
Trial Data ◽  
Regular Care ◽  
Joint Distraction

scholarly journals Knee joint distraction as standard of care treatment for knee osteoarthritis: a comparison with clinical trial patients

2019 ◽  
Vol 27 ◽  
pp. S515-S516 ◽  
Author(s):  
M. Jansen ◽  
S.C. Mastbergen ◽  
M.D. Van Empelen ◽  
E.C. Kester ◽  
F.P. Lafeber ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Standard Of Care ◽  
Care Treatment ◽  
Joint Distraction ◽  
Standard Of Care Treatment

Clinical outcomes of knee joint distraction combined with marrow stimulation procedures for patients with advanced knee osteoarthritis

The Knee ◽  
2021 ◽  
Vol 33 ◽  
pp. 342-350
Author(s):  
Nobuo Adachi ◽  
Seiju Hayashi ◽  
Atsuo Nakamae ◽  
Masakazu Ishikawa ◽  
Goki Kamei ◽  
...  
Keyword(s):  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Clinical Outcomes ◽  
Marrow Stimulation ◽  
Joint Distraction

scholarly journals Combination of Intra-Articular and Intraosseous Injections of Platelet Rich Plasma for Severe Knee Osteoarthritis: A Pilot Study

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Mikel Sánchez ◽  
Diego Delgado ◽  
Pello Sánchez ◽  
Emma Muiños-López ◽  
Bruno Paiva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Fluid ◽  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Subchondral Bone ◽  
Platelet Rich Plasma ◽  
Joint Function ◽  
Novel Approach ◽  
New Strategy

The aim of this study was to assess a novel approach to treating severe knee osteoarthritis by targeting synovial membrane, superficial articular cartilage, synovial fluid, and subchondral bone by combining intra-articular injections and intraosseous infiltrations of platelet rich plasma. We explored a new strategy consisting of intraosseous infiltrations of platelet rich plasma into the subchondral bone in combination with the conventional intra-articular injection in order to tackle several knee joint tissues simultaneously. We assessed the clinical outcomes through osteoarthritis outcome score (KOOS) and the inflammatory response by quantifying mesenchymal stem cells in synovial fluid. There was a significant pain reduction in the KOOS from baseline(61.55±14.11)to week 24(74.60±19.19), after treatment(p=0.008), in the secondary outcomes (symptoms,p=0.004; ADL,p=0.022; sport/rec.,p=0.017; QOL,p=0.012), as well as VAS score (p<0.001) and Lequesne Index(p=0.008). The presence of mesenchymal stem cells in synovial fluid and colony-forming cells one week after treatment decreased substantially from7.98±8.21 MSC/μL to4.04±5.36 MSC/μL(p=0.019)and from601.75±312.30to139.19±123.61  (p=0.012), respectively. Intra-articular injections combined with intraosseous infiltrations of platelet rich plasma reduce pain and mesenchymal stem cells in synovial fluid, besides significantly improving knee joint function in patients with severe knee osteoarthritis. This trial is registered on EudraCT with the number2013-003982-32.

scholarly journals Age-Related Alterations Affecting the Chondrogenic Differentiation of Synovial Fluid Mesenchymal Stromal Cells in an Equine Model

Cells ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1116 ◽  
Author(s):  
Mazzotti ◽  
Teti ◽  
Falconi ◽  
Chiarini ◽  
Barboni ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Knee Joint ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Chondrogenic Differentiation ◽  
Cellular Aging ◽  
Synovial Joint ◽  
Human Knee Joint ◽  
Age Related

Osteoarthritis is a degenerative disease that strongly correlates with age and promotes the breakdown of joint cartilage and subchondral bone. There has been a surge of interest in developing cell-based therapies, focused particularly on the use of mesenchymal stromal cells (MSCs) isolated from adult tissues. It seems that MSCs derived from synovial joint tissues exhibit superior chondrogenic ability, but their unclear distribution and low frequency actually limit their clinical application. To date, the influence of aging on synovial joint derived MSCs’ biological characteristics and differentiation abilities remains unknown, and a full understanding of the mechanisms involved in cellular aging is lacking. The aim of this study was therefore to investigate the presence of age-related alterations in synovial fluid MSCs and their influence on the potential ability of MSCs to differentiate toward chondrogenic phenotypes. Synovial fluid MSCs, isolated from healthy equine donors from 3 to 40 years old, were cultured in vitro and stimulated towards chondrogenic differentiation for up to 21 days. An equine model was chosen due to the high degree of similarity of the anatomy of the knee joint to the human knee joint and as spontaneous disorders develop that are clinically relevant to similar human disorders. The results showed a reduction in cell proliferation correlated with age and the presence of age-related tetraploid cells. Ultrastructural analysis demonstrated the presence of morphological features correlated with aging such as endoplasmic reticulum stress, autophagy, and mitophagy. Alcian blue assay and real-time PCR data showed a reduction of efficiency in the chondrogenic differentiation of aged synovial fluid MSCs compared to young MSCs. All these data highlighted the influence of aging on MSCs’ characteristics and ability to differentiate towards chondrogenic differentiation and emphasize the importance of considering age-related alterations of MSCs in clinical applications.

scholarly journals Synovial fluid hyaluronan mediates MSC attachment to cartilage, a potential novel mechanism contributing to cartilage repair in osteoarthritis using knee joint distraction

2015 ◽  
Vol 75 (5) ◽  
pp. 908-915 ◽  
Author(s):  
Thomas G Baboolal ◽  
Simon C Mastbergen ◽  
Elena Jones ◽  
Stuart J Calder ◽  
Floris P J G Lafeber ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Knee Joint ◽  
Cartilage Repair ◽  
Joint Distraction

scholarly journals Changes in Cartilage Thickness and Denuded Bone Area after Knee Joint Distraction and High Tibial Osteotomy—Post-Hoc Analyses of Two Randomized Controlled Trials

2021 ◽  
Vol 10 (2) ◽  
pp. 368
Author(s):  
Mylène P. Jansen ◽  
Susanne Maschek ◽  
Ronald J. van Heerwaarden ◽  
Simon C. Mastbergen ◽  
Wolfgang Wirth ◽  
...  
Keyword(s):  
Knee Joint ◽  
Knee Osteoarthritis ◽  
High Tibial Osteotomy ◽  
Cartilage Thickness ◽  
Effect Sizes ◽  
Tibial Osteotomy ◽  
Bone Area ◽  
Cartilage Restoration ◽  
Joint Distraction ◽  
Post Hoc

High tibial osteotomy (HTO) and knee joint distraction (KJD) are joint-preserving treatments that unload the more affected compartment (MAC) in knee osteoarthritis. This post-hoc study compares two-year cartilage-thickness changes after treatment with KJD vs. HTO, and identifies factors predicting cartilage restoration. Patients indicated for HTO were randomized to KJD (KJDHTO) or HTO treatment. Patients indicated for total knee arthroplasty received KJD (KJDTKA). Outcomes were the MRI mean MAC cartilage thickness and percentage of denuded bone area (dABp) change two years after treatment, using radiographic joint space width (JSW) as the reference. Cohen’s d was used for between-group effect sizes. Post-treatment, KJDHTO patients (n = 18) did not show significant changes. HTO patients (n = 33) displayed a decrease in MAC cartilage thickness and an increase in dABp, but an increase in JSW. KJDTKA (n = 18) showed an increase in MAC cartilage thickness and JSW, and a decrease in dABp. Osteoarthritis severity was the strongest predictor of cartilage restoration. Kellgren–Lawrence grade ≥3 showed significant restoration (p < 0.01) after KJD; grade ≤2 did not. Effect sizes between severe KJD and HTO patients were large for MAC MRI cartilage thickness (d = 1.09; p = 0.005) and dABp (d = 1.13; p = 0.003), but not radiographic JSW (d = 0.28; p = 0.521). This suggests that in knee osteoarthritis patients with high disease severity, KJD may be more efficient in restoring cartilage thickness.

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