scholarly journals High-Level Patchoulol Biosynthesis in Artemisia annua L.

2021 ◽  
Vol 8 ◽  
Author(s):  
Xueqing Fu ◽  
Fangyuan Zhang ◽  
Yanan Ma ◽  
Danial Hassani ◽  
Bowen Peng ◽  
...  
Keyword(s):  
Growth And Development ◽  
Artemisia Annua ◽  
Glandular Trichomes ◽  
Subcellular Location ◽  
Farnesyl Diphosphate Synthase ◽  
Plant Growth And Development ◽  
Microbial Synthesis ◽  
Artemisia Annua L ◽  
High Level ◽  
Membrane Components

Terpenes constitute the largest class of secondary metabolites in plants. Some terpenes are essential for plant growth and development, membrane components, and photosynthesis. Terpenes are also economically useful for industry, agriculture, and pharmaceuticals. However, there is very low content of most terpenes in microbes and plants. Chemical or microbial synthesis of terpenes are often costly. Plants have the elaborate and economic biosynthetic way of producing high-value terpenes through photosynthesis. Here we engineered the heterogenous sesquiterpenoid patchoulol production in A. annua. When using a strong promoter such as 35S to over express the avian farnesyl diphosphate synthase gene and patchoulol synthase gene, the highest content of patchoulol was 52.58 μg/g DW in transgenic plants. When altering the subcellular location of the introduced sesquiterpene synthetase via a signal peptide, the accumulation of patchoulol was observably increased to 273 μg/g DW. This case demonstrates that A. annua plant with glandular trichomes is a useful platform for synthetic biology studies.

scholarly journals PERSEPSI MASYARAKAT TANI TERHADAP PROGRAM PEMBERDAYAAN PETANI MELALUI SOSIALISASI TANAMAN OBAT Artemisia annua L. DI KABUPATEN KEPAHIANG PROVINSI BENGKULU

Humanus ◽  
2014 ◽  
Vol 13 (2) ◽  
pp. 107
Author(s):  
Apri Andani ◽  
Yudhy Harini Bertham ◽  
Abimanyu Dipo Nusantara
Keyword(s):  
Medicinal Plants ◽  
Sea Level ◽  
Growth And Development ◽  
Artemisia Annua ◽  
Ordinal Scale ◽  
Willingness To Participate ◽  
Farming Communities ◽  
Artemisia Annua L ◽  
Qualitative Descriptive ◽  
Descriptive Method

The purpose of this study is to determine the level of farmers’ perception towards empowerment program of farmers through public introduction of Artemisia annua L. medicinal plants in Kepahiang District Bengkulu Province. The location is intentionally determined by considering the geographical eligibility factor for the growth and development of the plant Artemisia annua L., which is more than 1000 meters above sea level. The study is conducted in three villages; Bandung Baru, Tangsi Duren, and Sukasari. In this study, respondents are deliberately determined on the farmers’ willingness to participate in the dissemination program of the research results about the commodities. Data were analyzed using qualitative descriptive method using ordinal scale with scores to distinguish the response of the respondents to the question or statement. The results show that knowledge of farming communities has improved about Artemisia annua L. plants; previously they did not know at all about this plant. Besides being suitable for agro-climate, they also feel that the plant has benefited the family’s health, although not directly felt economically. Generally, the farmers are willing to inform others, join and participate in the process of Artemisia annua L. cultivation. Keywords: Perception, Empowerment, Medicinal Plants, Artemisia annua L

scholarly journals Keragaman Somaklonal untuk Perbaikan Tanaman Artemisia (Artemisia annua L.) melalui Kultur In Vitro

2016 ◽  
Vol 6 (1) ◽  
pp. 26 ◽  
Author(s):  
Endang G Lestari ◽  
Ragapadmi Purnamaningsih ◽  
Muhammad Syukur ◽  
Rosa Yunita
Keyword(s):  
Gamma Ray ◽  
Artemisia Annua ◽  
Economic Value ◽  
Artemisia Annua L ◽  
Department Of Health ◽  
Somaclonal Variability ◽  
Breeding Selection ◽  
The One ◽  
High Level

<p>Somaclonal Variability for the Improvement of Plants<br />Artemisia (Artemisia annua L.) by In Vitro Culture.<br />Endang G. Lestari, Rosa Yunita, and Ali Husni. Artemisia<br />annua L., a family member of Asteraceae, is medicinal<br />plants originated from China. The plant has been widely<br />used by the local people for malaria remedy. Its active substance,<br />artemisine, has been proved to hamper the malaria<br />bacteria incubation, Plasmodium sp. In accordance with the<br />WHO recomendation, the Department of Health of Indonesia<br />is now in the attempt of developing this plant as the<br />subtitute of chloroquin because of the malaria bacteria<br />resistance to this antidote. In Indonesia, the artemisine<br />content of the plant less than 0,5% is the crucial problem<br />leading no investors are interested in its economic value.<br />Therefore, Indonesian Medicinal and Spice Crops Research<br />Institute; BPTO Tawangmangu, Indonesian Institute of<br />Sciences; and PT Kimia Farma cooperate for obtaining the<br />prime clone by breeding, selection, as well as environmental<br />adaptation. In coping with the problem, ICABIOGRAD in the<br />collaboration with Bogor Agricultural University have<br />conducted the research for genetic improvement through<br />mutative induction and field selection. This research on<br />somaclonal variation. was conducted from Januari 2006 to<br />Juni 2008. Eksplan used for experiment were shoots radiated<br />with 10-100 Gy gamma ray. The result showed that the shoot<br />radiated with the dosage of 70-100 Gy was unable to grow.<br />On the other hand, the high level of multiplication was<br />acquired in the one radiated with 10-30 Gy. The optimum<br />radiation for somaclonal radiation was eventually gained<br />with 40-60 Gy. The somaclone lines with 10-60 Gy radiation<br />have been aclimatized and planted in Gunung Putri plot in<br />the elevation of 1545 asl. Artemisinin content at the high<br />biomases genotype is 0,49-0,52%.</p>

AaSPL9 affects glandular trichomes initiation by positively regulating expression of AaHD1 in Artemisia annua L.

Plant Science ◽  
2021 ◽  
pp. 111172
Author(s):  
Yilong He ◽  
Xueqing Fu ◽  
Ling Li ◽  
Xiaofen Sun ◽  
Kexuan Tang ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Glandular Trichomes ◽  
Artemisia Annua L

Mechanisms of ethylene biosynthesis and response in plants

2015 ◽  
Vol 58 ◽  
pp. 61-70 ◽  
Author(s):  
Paul B. Larsen
Keyword(s):  
Plant Growth ◽  
Growth And Development ◽  
Ethylene Biosynthesis ◽  
Unsaturated Hydrocarbon ◽  
Flower Senescence ◽  
Detailed Knowledge ◽  
Plant Growth And Development ◽  
Step Process ◽  
Ethylene Response ◽  
Ethylene Signalling

Ethylene is the simplest unsaturated hydrocarbon, yet it has profound effects on plant growth and development, including many agriculturally important phenomena. Analysis of the mechanisms underlying ethylene biosynthesis and signalling have resulted in the elucidation of multistep mechanisms which at first glance appear simple, but in fact represent several levels of control to tightly regulate the level of production and response. Ethylene biosynthesis represents a two-step process that is regulated at both the transcriptional and post-translational levels, thus enabling plants to control the amount of ethylene produced with regard to promotion of responses such as climacteric flower senescence and fruit ripening. Ethylene production subsequently results in activation of the ethylene response, as ethylene accumulation will trigger the ethylene signalling pathway to activate ethylene-dependent transcription for promotion of the response and for resetting the pathway. A more detailed knowledge of the mechanisms underlying biosynthesis and the ethylene response will ultimately enable new approaches to be developed for control of the initiation and progression of ethylene-dependent developmental processes, many of which are of horticultural significance.

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