Keragaman Somaklonal untuk Perbaikan Tanaman Artemisia (Artemisia annua L.) melalui Kultur In Vitro

Somaclonal Variability for the Improvement of Plants Artemisia (Artemisia annua L.) by In Vitro Culture. Endang G. Lestari, Rosa Yunita, and Ali Husni. Artemisia annua L., a family member of Asteraceae, is medicinal plants originated from China. The plant has been widely used by the local people for malaria remedy. Its active substance, artemisine, has been proved to hamper the malaria bacteria incubation, Plasmodium sp. In accordance with the WHO recomendation, the Department of Health of Indonesia is now in the attempt of developing this plant as the subtitute of chloroquin because of the malaria bacteria resistance to this antidote. In Indonesia, the artemisine content of the plant less than 0,5% is the crucial problem leading no investors are interested in its economic value. Therefore, Indonesian Medicinal and Spice Crops Research Institute; BPTO Tawangmangu, Indonesian Institute of Sciences; and PT Kimia Farma cooperate for obtaining the prime clone by breeding, selection, as well as environmental adaptation. In coping with the problem, ICABIOGRAD in the collaboration with Bogor Agricultural University have conducted the research for genetic improvement through mutative induction and field selection. This research on somaclonal variation. was conducted from Januari 2006 to Juni 2008. Eksplan used for experiment were shoots radiated with 10-100 Gy gamma ray. The result showed that the shoot radiated with the dosage of 70-100 Gy was unable to grow. On the other hand, the high level of multiplication was acquired in the one radiated with 10-30 Gy. The optimum radiation for somaclonal radiation was eventually gained with 40-60 Gy. The somaclone lines with 10-60 Gy radiation have been aclimatized and planted in Gunung Putri plot in the elevation of 1545 asl. Artemisinin content at the high biomases genotype is 0,49-0,52%.

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Anthelmintic activity of Artemisia annua L. extracts in vitro and the effect of an aqueous extract and artemisinin in sheep naturally infected with gastrointestinal nematodes

Parasitology Research ◽

10.1007/s00436-014-3891-z ◽

2014 ◽

Vol 113 (6) ◽

pp. 2345-2353 ◽

Cited By ~ 17

Author(s):

Aida C. Cala ◽

Jorge F. S. Ferreira ◽

Ana Carolina S. Chagas ◽

Javier M. Gonzalez ◽

Rodney A. F. Rodrigues ◽

...

Keyword(s):

Aqueous Extract ◽

Artemisia Annua ◽

Anthelmintic Activity ◽

Gastrointestinal Nematodes ◽

Artemisia Annua L

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How to Achieve High-Quality Oocytes? The Key Role of Myo-Inositol and Melatonin

International Journal of Endocrinology ◽

10.1155/2016/4987436 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 36

Author(s):

Salvatore Giovanni Vitale ◽

Paola Rossetti ◽

Francesco Corrado ◽

Agnese Maria Chiara Rapisarda ◽

Sandro La Vignera ◽

...

Keyword(s):

Assisted Reproductive Technologies ◽

Reproductive Technologies ◽

Oocyte Quality ◽

Fertilization Rate ◽

In Vitro Models ◽

The One ◽

High Level

Assisted reproductive technologies (ART) have experienced growing interest from infertile patients seeking to become pregnant. The quality of oocytes plays a pivotal role in determining ART outcomes. Although many authors have studied how supplementation therapy may affect this important parameter for both in vivo and in vitro models, data are not yet robust enough to support firm conclusions. Regarding this last point, in this review our objective has been to evaluate the state of the art regarding supplementation with melatonin and myo-inositol in order to improve oocyte quality during ART. On the one hand, the antioxidant effect of melatonin is well known as being useful during ovulation and oocyte incubation, two occasions with a high level of oxidative stress. On the other hand, myo-inositol is important in cellular structure and in cellular signaling pathways. Our analysis suggests that the use of these two molecules may significantly improve the quality of oocytes and the quality of embryos: melatonin seems to raise the fertilization rate, and myo-inositol improves the pregnancy rate, although all published studies do not fully agree with these conclusions. However, previous studies have demonstrated that cotreatment improves these results compared with melatonin alone or myo-inositol alone. We recommend that further studies be performed in order to confirm these positive outcomes in routine ART treatment.

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Toxoplasma gondii: Effects of Artemisia annua L. on susceptibility to infection in experimental models in vitro and in vivo

Experimental Parasitology ◽

10.1016/j.exppara.2009.04.010 ◽

2009 ◽

Vol 122 (3) ◽

pp. 233-241 ◽

Cited By ~ 34

Author(s):

Taísa Carrijo de Oliveira ◽

Deise A. Oliveira Silva ◽

Cristina Rostkowska ◽

Samantha Ribeiro Béla ◽

Eloisa A.V. Ferro ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Artemisia Annua ◽

Experimental Models ◽

Susceptibility To Infection ◽

Artemisia Annua L

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In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture

Genetics & Applications ◽

10.31383/ga.vol5iss2pp1-9 ◽

2021 ◽

Vol 5 (2) ◽

pp. 1

Author(s):

Tamara Ćetković ◽

Anja Haverić ◽

Lejla Čaluk Klačar ◽

Maida Hadžić Omanović ◽

Sanin Haverić

Keyword(s):

Peripheral Blood ◽

Artemisia Annua ◽

Neutral Red ◽

Cytotoxic Effects ◽

Artemisia Annua L ◽

Peripheral Blood Culture ◽

Pharmaceutical Form ◽

In Series ◽

Dose Dependent

The genus Artemisia (fam. Asteraceae) is one of the largest and widely distributed with around 500 species, majority used as aromatic and medicinal plants. Artemisia annua L. is widely used as a dietary spice, herbal tea, as a supplement, and in a non-pharmaceutical form for treatment of malaria and fever. It is orally consumed as capsules, extracts and tinctures and topically applied as an essential oil diluted in lotions and ointments. Artemisinin is the main constituent of Artemisia annua L. extracts. Since the discovery that the artemisinin is efficient in malaria treatment, there is also a growth in consumption of A. annua extracts for antitumour and even recently for antiviral treatments against SARS-CoV-2 infections. This study aimed to investigate genotoxic effect in peripheral blood culture and cytotoxic effects in cancer and normal cell lines, of commercially available A. annua L. tincture in series of dilutions. Both comet and neutral red uptake assays revealed dose-dependent genotoxicity and cytotoxicity of A. annua tincture dilutions. Comet assay revealed significantly increased DNA damage in peripheral blood cells while neutral-red assays showed increase in cytotoxicity (p<0.001) in both normal and cancer cell cultures treated with the lowest extract dilution compared to the highest one applied. Obtained results indicate caution needed in A. annua L. tincture use, especially when poorly diluted.

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