scholarly journals Optimization of Specific Productivity for Xylonic Acid Production by Gluconobacter oxydans Using Response Surface Methodology

2021 ◽  
Vol 9 ◽  
Author(s):  
Tao He ◽  
Chaozhong Xu ◽  
Chenrong Ding ◽  
Xu Liu ◽  
Xiaoli Gu
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Process Parameters ◽  
Acid Production ◽  
Production Efficiency ◽  
Biomass Concentration ◽  
Specific Productivity ◽  
Acid Fermentation ◽  
Main Challenge ◽  
Xylonic Acid

Large amounts of xylose cannot be efficiently metabolized and fermented due to strain limitations in lignocellulosic biorefinery. The conversion of xylose into high value chemicals can help to reduce the cost of commercialization. Therefore, xylonic acid with potential value in the construction industry offers a valuable alternative for xylose biorefinery. However, low productivity is the main challenge for xylonic acid fermentation. This study investigated the effect of three reaction parameters (agitation, aeration, and biomass concentration) on xylose acid production and optimized the key process parameters using response surface methodology The second order polynomial model was able to fit the experimental data by using multiple regression analysis. The maximum specific productivity was achieved with a value of 6.64 ± 0.20 g gx−1 h−1 at the optimal process parameters (agitation speed 728 rpm, aeration rate 7 L min−1, and biomass concentration 1.11 g L−1). These results may help to improve the production efficiency during xylose acid biotransformation from xylose.

Enhanced L-glutamic acid production by immobilized Corynebacterium glutamicum X680 using Response Surface Methodology

2021 ◽  
Vol 16 (12) ◽  
pp. 87-94
Author(s):  
Subhadeep Ganguly ◽  
Smaranika Pattnaik
Keyword(s):  
Response Surface Methodology ◽  
Glutamic Acid ◽  
Response Surface ◽  
Corynebacterium Glutamicum ◽  
Acid Production ◽  
Immobilized Cells ◽  
Lower Amount ◽  
Acid Fermentation ◽  
Statistical Tool ◽  
Glutamic Acid Production

L-glutamic acid is a non-essential amino acid largely used as flavor enhancer and food additive. It also has several therapeutic applications. Fermentation has gained superiority over its chemical synthesis as it produced stereo-specific isomer. Corynebacterium glutamicum is mostly used microorganism for Lglutamic acid fermentation. The study was dealing with optimization of L-glutamic acid production by immobilized mutant Corynebacterium glutamicum X680 in calcium alginate beads using response surface methodology as effective statistical tool. Among several parameters studied, pH, inoculums size, incubation time, concentration of sodium alginate, agitation and cell:alginate ration showed the most significant effect. Immobilized cells produced significantly (p<0.01) lower amount of L-glutamic acid (24.3mg/ml) compared to the production by free cells (27.6mg/ml). However, reusability of the beads minimized production cost and hence conferred benefit as far as the market economy is concerned.

Fiber laser processing of GFRP composites and multi-objective optimization of the process using response surface methodology

2018 ◽  
Vol 53 (11) ◽  
pp. 1459-1473 ◽  
Author(s):  
Shiva Dayal Rao B ◽  
Abhijeet Sethi ◽  
Alok Kumar Das
Keyword(s):  
Response Surface Methodology ◽  
Fiber Laser ◽  
Response Surface ◽  
Process Parameters ◽  
Heat Affected Zone ◽  
Cutting Speed ◽  
Kerf Width ◽  
Laser Irradiance ◽  
Input Process ◽  
Cut Surface

In the present investigation, a continuous wave fiber laser with maximum power of 400 W was used to cut a glass fiber reinforced plastic sheet of 4.56 mm thickness using Nitrogen as assisting gas. The influence processing parameters such as laser irradiance, gas pressure, and cutting speed on the cut surface quality were investigated by using response surface methodology. The different responses of laser cut surface such as upper kerf width, taper percentage along the cut depth, and heat-affected zone on the top surface were measured to analyze the influence of input process parameters on the responses. A statistical analysis on the obtained results was conducted and found that the optimum values of different input process parameters were laser irradiance: 8.28 × 105 watt/cm2, cutting speed: 600 mm/min and assisting gas pressure: 7.84 bar. The corresponding values of responses were upper kerf width: 177.4 µm, taper 0.73%, and heat-affected zone on top surface: 109.23 µm. The confirmation experiments were conducted with the obtained optimum parameter setting and observed that the predicted values and experimental values for upper kerf width, taper percentage and top surface heat-affected zone were within the error limits of 2.52%, 1.84%, and 0.45%, respectively. Furthermore, damages like loose fibers, interlayer fractures, evaporation of matrix material and fiber breakages were observed.

scholarly journals Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology

2011 ◽  
Vol 42 (2) ◽  
pp. 658-667 ◽  
Author(s):  
Daniela A. Viana Marques ◽  
Márcia N. Carneiro Cunha ◽  
Janete M. Araújo ◽  
José L. Lima-Filho ◽  
Attilio Converti ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Clavulanic Acid ◽  
Response Surface ◽  
Acid Production
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