Identification and Characterization of an O-Succinyl-L-Homoserine Sulfhydrylase From Thioalkalivibrio sulfidiphilus

L-methionine is an important natural amino acid with broad application prospects. A novel gene encoding the enzyme with the ability to catalyze O-succinyl-L-homoserine (OSH) to L-methionine was screened and characterized. The recombinant O-succinyl-L-homoserine sulfhydrylase from Thioalkalivibrio sulfidiphilus (tsOSHS) exhibited maximum activity at 35°C and pH 6.5. OSHS displayed an excellent thermostability with a half-life of 21.72 h at 30°C. Furthermore, the activity of OSHS increased 115% after Fe2+ added. L-methionine was obtained with a total yield reaching 42.63 g/L under the concentration of O-succinyl-L-homoserine 400 mM (87.6 g/L). These results indicated that OSHS is a potential candidate for applying in the large-scale bioproduction of L-methionine.

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Identification and characterization of YLR328W, theSaccharomyces cerevisiaestructural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme

FEBS Letters ◽

10.1016/s0014-5793(99)00852-2 ◽

1999 ◽

Vol 455 (1-2) ◽

pp. 13-17 ◽

Cited By ~ 39

Author(s):

Monica Emanuelli ◽

Francesco Carnevali ◽

Maria Lorenzi ◽

Nadia Raffaelli ◽

Adolfo Amici ◽

...

Keyword(s):

Recombinant Enzyme ◽

Gene Encoding ◽

Identification And Characterization

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Identification and characterization of the gene encoding an extracellular protease from haloarchaeon Halococcus salifodinae

Microbiological Research ◽

10.1016/j.micres.2020.126468 ◽

2020 ◽

Vol 236 ◽

pp. 126468 ◽

Cited By ~ 1

Author(s):

Jing Hou ◽

Dong Han ◽

Yao Zhou ◽

Yang Li ◽

Heng-Lin Cui

Keyword(s):

Extracellular Protease ◽

Gene Encoding ◽

Identification And Characterization

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Large-Scale Identification and Characterization of Hematopoietic Cell Transplant Recipients and Their Donors With Inherited Chromosomally Integrated Human Herpesvirus 6

Open Forum Infectious Diseases ◽

10.1093/ofid/ofw194.22 ◽

2016 ◽

Vol 3 (suppl_1) ◽

Author(s):

Joshua Hill ◽

Ruth Hall Sedlak ◽

Amalia Magaret ◽

Anna Mikhaylova ◽

Meei-Li Huang ◽

...

Keyword(s):

Large Scale ◽

Human Herpesvirus ◽

Hematopoietic Cell ◽

Cell Transplant ◽

Transplant Recipients ◽

Human Herpesvirus 6 ◽

Hematopoietic Cell Transplant ◽

Herpesvirus 6 ◽

Identification And Characterization

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Identification and characterization of a novel gene encoding myb-box binding zinc finger protein in Gossypium arboreum

Biologia Plantarum ◽

10.1007/s10535-012-0255-3 ◽

2012 ◽

Vol 56 (4) ◽

pp. 641-647 ◽

Cited By ~ 5

Author(s):

M. Zahur ◽

A. Maqbool ◽

M. Irfan ◽

A. Jamal ◽

N. Shahid ◽

...

Keyword(s):

Zinc Finger ◽

Zinc Finger Protein ◽

Gossypium Arboreum ◽

Gene Encoding ◽

Novel Gene ◽

Finger Protein ◽

Identification And Characterization

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Genome-Wide Identification and Characterization of Long Non-Coding RNAs in Peanut

Genes ◽

10.3390/genes10070536 ◽

2019 ◽

Vol 10 (7) ◽

pp. 536 ◽

Cited By ~ 2

Author(s):

Xiaobo Zhao ◽

Liming Gan ◽

Caixia Yan ◽

Chunjuan Li ◽

Quanxi Sun ◽

...

Keyword(s):

Large Scale ◽

Target Genes ◽

Sequencing Data ◽

Regulatory Processes ◽

Genome Wide ◽

Non Coding Rnas ◽

Identification And Characterization ◽

Lower Expression ◽

Weighted Correlation

Long non-coding RNAs (lncRNAs) are involved in various regulatory processes although they do not encode protein. Presently, there is little information regarding the identification of lncRNAs in peanut (Arachis hypogaea Linn.). In this study, 50,873 lncRNAs of peanut were identified from large-scale published RNA sequencing data that belonged to 124 samples involving 15 different tissues. The average lengths of lncRNA and mRNA were 4335 bp and 954 bp, respectively. Compared to the mRNAs, the lncRNAs were shorter, with fewer exons and lower expression levels. The 4713 co-expression lncRNAs (expressed in all samples) were used to construct co-expression networks by using the weighted correlation network analysis (WGCNA). LncRNAs correlating with the growth and development of different peanut tissues were obtained, and target genes for 386 hub lncRNAs of all lncRNAs co-expressions were predicted. Taken together, these findings can provide a comprehensive identification of lncRNAs in peanut.

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Identification and Characterization of a Porcine Torovirus

Journal of Virology ◽

10.1128/jvi.72.5.3507-3511.1998 ◽

1998 ◽

Vol 72 (5) ◽

pp. 3507-3511 ◽

Cited By ~ 59

Author(s):

A. Kroneman ◽

L. A. H. M. Cornelissen ◽

M. C. Horzinek ◽

R. J. de Groot ◽

H. F. Egberink

Keyword(s):

Longitudinal Study ◽

Neutralizing Antibodies ◽

Rt Pcr ◽

Gene Encoding ◽

Nucleocapsid Gene ◽

Sequence Identity ◽

Young Pigs ◽

Porcine Torovirus ◽

Identification And Characterization

ABSTRACT A porcine torovirus (PoTV) was identified and characterized; it is a novel member of the genus Torovirus (familyCoronaviridae, order Nidovirales), closely related to but clearly distinct from the already recognized equine torovirus (ETV) and bovine torovirus (BoTV) representatives. Immunoelectron microscopy of feces from piglets revealed elongated, 120- by 55-nm particles which were recognized by a torovirus-specific antiserum. Amplification by reverse transcriptase (RT) PCR with primers designed to detect conserved regions (on the basis of the genomes of BoTV strain Breda and ETV strain Berne) resulted in the identification of the 489-bp nucleocapsid gene, encoding a 18.7-kDa protein. The sequence identity in this region between PoTV and both ETV and BoTV was only about 68%, whereas the latter two show 81% identity. Neutralizing antibodies directed against ETV were found in sera of adult and young pigs. In all 10 herds sampled, seropositive animals were present, and 81% of randomly selected adult sows possessed antibodies. A longitudinal study with RT PCR showed that piglets shed virus in the feces for 1 or more days, starting 4 to 14 days after weaning.

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