scholarly journals Antibiotic Resistance Pattern of Extended Spectrum Beta Lactamase Producing Escherichia coli Isolated From Patients With Urinary Tract Infection in Morocco

2021 ◽  
Vol 11 ◽  
Author(s):  
Mohamed Kettani Halabi ◽  
Fatima Azzahra Lahlou ◽  
Idrissa Diawara ◽  
Younes El Adouzi ◽  
Rabiaa Marnaoui ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples ◽  
Resistance Pattern ◽  
E Coli ◽  
Antibiotic Resistance Pattern ◽  
Extended Spectrum

Extended-spectrum β-lactamases producing Escherichia coli (ESBL-EC) lend resistance to most β-lactam antibiotics. Because of limited treatment options, ESBL-EC infections are generally more difficult to treat, leading to higher hospital costs, reduced rates of microbiological and clinical responses, and a threat to the patient’s life. This study aimed to determine the antibiotic resistance pattern of ESBL-EC isolated from patients with urinary tract infection in Morocco. This retrospective laboratory-based study was conducted at Cheikh Khalifa International University Hospital, Casablanca, from January 2016 to June 2019. A total of 670 urine samples were collected from urinary tract infection patients and processed by standard microbiological methods. In vitro susceptibility testing to different antibiotics of all identified isolates of Escherichia coli (E. coli) was performed following Kirby–Bauer’s disc diffusion method on Mueller–Hinton Agar according to the EUCAST standards. The reviewing of ESBL-EC was confirmed by the appearance of a characteristically shaped zone referred to as a “champagne cork” using the Combined Disk Test. Among a total of 438 E. coli isolated from nonrepetitive urine samples, two hundred fifty-nine (59%) were ESBL-EC, of which 200 (77%) were isolated from adult patients (over the age of 50) and the majority were female. All ESBL-EC isolates were resistant to third-generation cephalosporin and quinolones and sensitive to carbapenem and fosfomycin. Knowledge of antimicrobial resistance patterns in ESBL-EC, the major pathogen associated with urinary tract infection, is indispensable as a guide in choosing empirical antimicrobial treatment.

scholarly journals Emerging antibiotic resistance pattern of urinary tract infection (UTI) due to Escherichia Coli (E.Coli).

2021 ◽  
Vol 28 (12) ◽  
pp. 1804-1811
Author(s):  
Salman Azhar ◽  
Talha Munir ◽  
Mian Sajjad Ahmed ◽  
Wasif Baig ◽  
Ahmed Nawaz Baber ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Culture ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
E Coli ◽  
Antibiotic Resistance Pattern ◽  
Culture And Sensitivity

Objective: To determine the emerging antibiotic resistance pattern of adult patients of MDR UTI cases caused by E.Coli. Study Design: Retrospective Data. Setting: Departments of Medicine and Obstetrics & Gynecology, Madinah Teaching Hospital / University Medical and Dental College, Faisalabad. Period: August 2016 to Sep 2018. Material & Methods: This retrospective study included 187 patients meeting the operational definition of multi-drug resistance cases of urinary tract infection caused by E. coli. All cases either male or female of age above 15 years with positive reports of urine culture and sensitivity for E.coli were included in the study. Along with the demographic variables urine WBC’s, results of urine culture and sensitivity were noted. Antibiotic sensitivity pattern of all the E.coli resistant UTI cases was analyzed. After identification of the microbe, antimicrobial sensitivity was accessed using Kirby–Bauer disc diffusion method on the Mueller–Hinton agar. The antimicrobial susceptibility test was performed against E. coli strains by using the following antibiotics: Piperacilin, Amoxicilin-Calvulanic, Pipmedic Acid, Cefipime, Ceftrixone, Cefuroxime, Cefoperazone-sulbactam, Ceftazidime, Cephradine, Ciprofloxacin, Ofloxacin, Levofloxacin, ofloxacin, Nitrofurantoin, Amikacin, Tombramycin, Gentamicin, Linezolid, Doxycycline, Sulphamethoxazole, Imipenem, Meropenem, Aztreonam in order to obtain an antibiogram. Results: In our data, 66.8% (n=125) were MDR UTI, and more than half of these cases were 63.2% (n=79) were females. Among the total 187 cases, 97.3% patients were resistant to most of the beta-lactam antibiotics, 95.7% were resistant to most of the quinolones and 68.4% were resistant to most of the aminoglycosides. Conclusion: This increasing antibiotic resistance is very alarming and steps should be taken to reduce the misuse of antibiotics. Studies should be done to control the risk factors leading to E. coli UTI. By following proper pharmacological guidelines, principles to use antibiotics and manage outpatient cases of UTIs, the incidence of MDR UTI can be controlled.

scholarly journals Antibiotic resistance pattern in patients with urinary tract infection: an observational study

2019 ◽  
Vol 9 (1) ◽  
pp. 195
Author(s):  
Rupa Devi Mallam ◽  
Usha Kiran P.
Keyword(s):  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Observational Study ◽  
Nalidixic Acid ◽  
Resistance Pattern ◽  
Clinical Practices ◽  
E Coli ◽  
Antibiotic Resistance Pattern

Background: Urinary tract infection (UTI) is one of the most common diseases caused by bacteria in communities and hospital settings. With the irrational prescription of antibiotics and their misuse leads to constant increase in resistance. This study aims to evaluate the spectrum and antibiotic resistance pattern of uropathogens and to provide a basis for appropriate antimicrobial therapy in patients with UTI.Methods: A retrospective observational study was carried out for a period of 6 months from April 2018 to September 2018 at general hospital Kakinada. Data of 282 positive urine culture reports and their antibiotic susceptibility test results were collected from the records of the Microbiology department and were analyzed and depicted in percentages. Sample processing, identification of organisms, and pattern of antimicrobial sensitivity were carried out as per the Clinical and Laboratory Standards Institute guidelines.Results: Out of 282 positive reports, Escherichia coli was the most common isolated uropathogen with a total of 148 (52.4%) followed by Klebsiella 72 (26%), 20 (7.9%) each of Citrobacter and pseudomonas aeruginosa. E. coli were resistant to nalidixic acid (59.5%), cotrimoxazole (58.1%), ciprofloxacin (54%) amoxyclav (52.7%), ampicilin (45.9%), cefatoxime (37.8%), cefaperazone and salbactum (25.3%). Klebsiella were resistant to amoxyclav (77.7%), cotrimazole (50%), nalidixic acid (41.6%), ampicilin (44.4%), cefatoxime (27.7%), ciprofloxacin (25%). E. coli and Klebsiella were highly susceptible to nitrofurantoin, cefaperazone and salbactum, piperacillin and tazobactum, amikacin, imipenem, gentamicin.Conclusions: The majority of the isolated bacteria were resistant to many antibiotics commonly used in clinical practices. So prior culture reports and institutional antibiograms are necessary for prescribing antibiotics rationally.

scholarly journals Comparative Antibiogram of Escherichia Coli Isolated from the Urinary Tract Infection in Patients from Tertiary Care Hospital

2021 ◽  
pp. 123-128
Author(s):  
Nandkishor Bankar ◽  
Dhruba Hari Chandi ◽  
Praful Patil ◽  
Gaurav Mahajan
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Tertiary Care ◽  
Resistance Pattern ◽  
Care Hospital ◽  
Biochemical Tests ◽  
E Coli

Introduction: UTI occurs when bacteria that live inside intestine find their way into the urinary tract through the urethra. Women are familiar to be a lot of at risk of UTI than men, with a five hundredth probability of a UTI in their life. Aim: The aim of this study was to find the antibiotic resistance pattern in Escherichia coli isolated from urinary tract infection with commonly found antibiotics among different age and sex groups. Materials and Methods: All positive urine samples for Escherichia coli obtained from patients were included. On the basis of colony morphology and biochemical tests Isolates were identified. The antibiogram profile of the isolates was determined by commonly used antibiotics. Result: In this studied, total 250 patients with UTI of both sexes were included and divided into age group. Samples processed only 250 samples with the growth of E. coli were included in this studied. The isolated E.coli showed more resistant to some degree to all the antibiotics tested.  The greatest resistance was shown against Amoxycillin (15.2% isolated were sensitive) and the least resistance was shown against Imipenem (96.4% isolated were sensitive). Conclusion: The pattern of antibiotic resistance varies from place to place, region to region, and country to country. Antimicrobial resistance should be monitored on a regular basis in both the community and hospital settings.

scholarly journals Prevalence and antibiotic resistance pattern of extended spectrum beta lactamase producing Escherichia coli isolated from urinary tract infection

2018 ◽  
Vol 8 (2) ◽  
pp. 78-81 ◽  
Author(s):  
Mohsen Rajabnia ◽  
Mohammad Saad Forghani ◽  
Sabah Hasani ◽  
Mohammad Bahadoram ◽  
Mahsa Mohammadi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Resistance Pattern ◽  
Beta Lactamase ◽  
Esbl Producer ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli

Introduction: Urinary tract infection (UTI) due to extended spectrum beta-lactamase (ESBL)-producing bacteria including Escherichia coli has become widespread. Studies have shown a trend toward higher mortality, longer hospitalization, greater hospital expenses and reduced rates of clinical and microbiologic response in ESBL UTI. Objectives: The aim of this study is to determinate the prevalence and antibiotic resistance pattern of ESBL producing E. coli isolated from UTI. Patients and Methods: This cross-sectional study was conducted on 3126 samples. Urine specimens were cultured on Eosin Methylene Blue (EBM) and blood agar. The disk diffusion standard method (Kirby Bauer) was used to test the susceptibility of the drug on MullerHinton agar plates and results were reviewed based on Clinical and Laboratory Standards Institute (CLSI) criteria. The reviewing of ESBL-producing uropathogens was carried out using Combined Disk Test (CDT) by using cefotaxime (CTX; 30 µg) and cefotaximeclavulanic acid (CTX; 30 µg /CA:10 µg) disks and CLSI protocol. Results: Out of 291 E. coli isolates, 108 (37.11%) are ESBL-producer and 183 (62.89%) are non–ESBL-producer. Among ESBL-producing E. coli, the highest antibiotic resistance was observed with cefotaxime (100%), amoxicillin (97.22%) and piperacillin (96.3%) and the highest antibiotic sensitivity was observed with meropenem (93.5%), nitrofurantoin (81.48%) and gentamicin (55.56%). Conclusion: We recommended that cephalosporins, penicillins and cotrimoxazole are not suggested in the treatment of ESBL-producing E. coli. On the other hand, carbapenems as a first line and aminoglycosides as the next step in the treatment of ESBL-producing E. coli are recommended.

scholarly journals Multiple-Antibiotic Resistance Mediated by Plasmids and Integrons in Uropathogenic Escherichia coli and Klebsiella pneumoniae

1970 ◽  
Vol 24 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Taslima Taher Lina ◽  
Sabita Rezwana Rahman ◽  
Donald James Gomes
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Klebsiella Pneumoniae ◽  
High Rate ◽  
E Coli ◽  
Class 1

Antibiotic resistance in urinary tract infection (UTI) is a growing public health problem in the world. In this study, a total of 182 uropathogens were isolated from patients with symptoms of urinary tract infection (UTI). Escherichia coli (88%) was the most prevalent isolate, while Klebsiella pneumoniae was recovered from 12% cases. The male/female ratio was 1:3. About 56% female and 51% male patients belonged to the age group >40 years. The antibiotic resistance rates of the isolates to fifteen different drugs were investigated. E. coli and K. pneumoniae showed variable pattern of susceptibility. The percentage of resistance to different drugs was higher in E. coli isolates compared to that of K. pneumoniae. Among the total number of isolates about 87% were resistant to at least three commonly used antibiotics. All the isolates were sensitive to imipenem. Analysis of the plasmid DNA had shown that the plasmid pattern was very diverse in both E. coli and K. pneumoniae. All the isolates contained multiple numbers of plasmid ranging from 1.0 to >140 MDa. Middleranged plasmids (30 to 80 MDa), the transferable resistance plasmids, were found to be present in 86% E. coli and 85% K. pneumoniae isolates. The strong association observed between plasmid profiles and drug resistance patterns suggest that plasmids other than the common plasmids may have epidemiological significance. The presence of class 1 and class 2 integrons were also investigated. A relatively high occurrence of class 1 integrons, that are associated with lateral transfer of antibacterial resistance genes, was observed in K. pneumoniae (88%) than in E. coli isolates (54%). Class 2 integrons were not found in any of the E. coli and K. pneumoniae isolates. These results show the high rate of drug resistance and the presence of high rate of transferable elements in these MDR isolates. Keywords: Uropathogens, Escherichia coli, Klebsiella pneumoniae, Multidrug-resistant (MDR) bacteria, Plasmid profiles, IntegronsDOI: http://dx.doi.org/10.3329/bjm.v24i1.1231 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 19-23

scholarly journals Antibiotic sensitivity pattern of bacterial isolates from urine samples of admitted patients with urinary tract infection in a tertiary care teaching hospital of Tripura, India: a hospital record based study

2018 ◽  
Vol 7 (4) ◽  
pp. 585
Author(s):  
Uttam K. Das ◽  
Prithul Bhattacharjee ◽  
Shubhaleena Debnath ◽  
Maitrayee Chakraborty ◽  
Ranjib Ghosh ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Teaching Hospital ◽  
Antibiotic Sensitivity ◽  
Urine Samples ◽  
Hospital Record ◽  
E Coli ◽  
Sensitivity Pattern

Background: Urinary tract infection (UTI) being one of the most common and a serious health problem both in the community and hospital settings each year worldwide, the emergence of antibiotic resistance in the management of UTI is a serious public health issue. The present study will analyse the antimicrobial sensitivity pattern of pathogens isolated from the urine samples of admitted patients suffering from UTI in Tripura Medical College and Dr. B.R. Ambedkar Memorial Teaching Hospital (TMC).Methods: This was a hospital record-based study. The urine samples of clinically diagnosed UTI patients admitted in various departments of the hospital during the study period were included. The reports of culture and sensitivity testing of the samples were collected. The results were interpreted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI).Results: During the 12-month study period, a total of 752 urine samples were analysed. Enterococcus (43.75%) was the most frequently isolated bacteria, followed by E. coli (28.45%) and Klebsiella (14.89%). Enterococcus was highly sensitive (p<0.001) to vancomycin (95.33%), E. coli was mostly sensitive to nitrofurantoin (83.65%) and Klebsiella mainly sensitive to imipenem (75.49%).Conclusions: The study showed that positive urine culture with the antibiotic sensitivity of the isolates is very important for antimicrobial therapy, as antibiotic resistance is a worldwide problem which causes ineffectiveness of treatment.

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