Aging and chronic administration of serotonin-selective reuptake inhibitor citalopram upregulate Sirt4 gene expression in the preoptic area of male mice

Abstract In rodents, kisspeptin neurons in the rostral periventricular area of the third ventricle (RP3V) of the preoptic area are considered to provide a major stimulatory input to the GnRH neuronal network that is responsible for triggering the preovulatory LH surge. Noradrenaline (NA) is one of the main modulators of GnRH release, and NA fibers are found in close apposition to kisspeptin neurons in the RP3V. Our objective was to interrogate the role of NA signaling in the kisspeptin control of GnRH secretion during the estradiol induced LH surge in ovariectomized rats, using prazosin, an α1-adrenergic receptor antagonist. In control rats, the estradiol-induced LH surge at 17 hours was associated with a significant increase in GnRH and kisspeptin content in the median eminence with the increase in kisspeptin preceding that of GnRH and LH. Prazosin, administered 5 and 3 hours prior to the predicted time of the LH surge truncated the LH surge and abolished the rise in GnRH and kisspeptin in the median eminence. In the preoptic area, prazosin blocked the increases in Kiss1 gene expression and kisspeptin content in association with a disruption in the expression of the clock genes, Per1 and Bmal1. Together these findings demonstrate for the first time that NA modulates kisspeptin synthesis in the RP3V through the activation of α1-adrenergic receptors prior to the initiation of the LH surge and indicate a potential role of α1-adrenergic signaling in the circadian-controlled pathway timing of the preovulatory LH surge.

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Hypothalamic regulation of reproduction under the chronic influence of toluene and melatonin

Journal of obstetrics and women s diseases ◽

10.17816/jowd89476 ◽

2021 ◽

Vol 50 (2) ◽

pp. 42-46

Author(s):

G. O. Kerkeshko

Keyword(s):

Biogenic Amines ◽

Preoptic Area ◽

Chronic Administration ◽

Female Rats ◽

Control Group ◽

Simultaneous Administration ◽

Chronic Effect ◽

Circadian Variations ◽

Hypothalamic Regulation ◽

Maximal Permissible Concentration

Experiments on chronic administration of melatonin with and without chronic inhalation of toluene dosed at both maximal permissible concentration (50 mg/ml) and limited chronical range (500 mg/m3) have been carried out on female rats to discover their effects on biogenic amines system in hypothalamic structures related to gonadoliberin synthesis and secretion - preoptic area (PA) and median eminence (ME). Contents of biogenic amines in ME and especially in PA have been shown to have circadian variations with maximum in the morning in control group of rats.The chronic effect of synchronizing agent melatonin (administered dissolved in drinking water in concentration of 10 pg/m l, at night during 2 months) on neotransmitters and their circadian variations in both hypothalamic structures proved surprisingly to be much alike the effect of toluene. Both chemicals cause the disturbances of normal circadian variations o f norepinephrine, dopamine and serotonine in PA and dopamine in ME. The simultaneous administration of toluene and melatonin showed likewise no synchronizing ability of the latter under the conditions described.

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Gene expression profiling of the olfactory tissues of sex-separated and sex-combined female and male mice

Scientific Data ◽

10.1038/sdata.2018.260 ◽

2018 ◽

Vol 5 (1) ◽

Cited By ~ 3

Author(s):

Stephen W. Santoro ◽

Susanne Jakob

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Expression Profiling ◽

Male Mice

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Reduction of serotonergic gene expression in the raphe nuclei of the midbrain under positive fighting experience in male mice

Advances in Bioscience and Biotechnology ◽

10.4236/abb.2013.410a3005 ◽

2013 ◽

Vol 04 (10) ◽

pp. 36-44 ◽

Cited By ~ 9

Author(s):

Dmitry A. Smagin ◽

Ul’yana A. Boyarskikh ◽

Natalya P. Bondar ◽

Maxim L. Filipenko ◽

Natalia N. Kudryavtseva

Keyword(s):

Gene Expression ◽

Raphe Nuclei ◽

Male Mice ◽

Raphé Nuclei

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c-Fos gene expression pattern in the hypothalamus and the preoptic area of defeminized rats

Brain Research ◽

10.1016/s0006-8993(00)02244-7 ◽

2000 ◽

Vol 867 (1-2) ◽

pp. 100-106 ◽

Cited By ~ 11

Author(s):

Isabel Arrieta ◽

Ignacio Camacho-Arroyo ◽

C.Adriana Mendoza-Rodrı́guez ◽

Marco A. Cerbón

Keyword(s):

Gene Expression ◽

Expression Pattern ◽

Preoptic Area ◽

Gene Expression Pattern

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Oxtr/TRPV1 expression and acclimation of skeletal muscle to cold-stress in male mice

Journal of Endocrinology ◽

10.1530/joe-20-0346 ◽

2021 ◽

Author(s):

Elena Conte ◽

Adele Romano ◽

Michela De Bellis ◽

Maria Luisa De Ceglia ◽

Maria Rosaria Carratù ◽

...

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Cold Stress ◽

Transient Receptor Potential ◽

Receptor Potential ◽

Transient Receptor Potential Vanilloid ◽

Male Mice ◽

Transient Receptor ◽

Immunohistochemical Studies ◽

Circulating Levels

We explored the involvement of Oxytocin receptor (Oxtr)/ Transient-receptor-potential-vanilloid-1 (TRPV1) genes and Oxytocin (Oxt) on the adaptation of skeletal muscle to cold stress challenge in mice. Oxtr expression in hypothalamic paraventricular (PVN), supraoptic nuclei (SON), and hippocampus (HIPP) were evaluated by immunohistochemistry in parallel with the measurement of circulating Oxt. The Oxtr and TRPV1 gene expression in Soleus (SOL) and Tibialis Anterior (TA) muscles were investigated by RT-PCR. Histological studies of the cardiac muscle after cold stress were also performed. Male mice (n=15) were divided into controls maintained at room temperature (RT=24°C), exposed to cold stress (CS) at T=4°C for 6 hours (6h), and 5 days (5d). Immunohistochemical studies showed that Oxtr protein expression increased by 2-fold (p=0.01) in PVN and by 1.5-fold (p=0.0001) in HIPP after 6h and 5d CS, but decreased by 2-fold (p=0.026) in SON at 5d. Both Oxtr and TRPV1 gene expression increased after 6h and 5d CS in SOL and TA muscles. Oxtr vs TRPV1 gene expression in SOL and TA muscles evaluated by regression analysis was linearly correlated following CS at 6h and 5d but not at control temperature of 24+1°C, supporting the hypothesis of coupling between these genes. The circulating levels of Oxt are unaffected after 6h CS but decreased by 0.2-fold (p=0.0141) after 5d CS. This is the first report that Oxtr and TRPV1 expression are upregulated in response to cold acclimation in skeletal muscle. The up-regulation of Oxtr in PVN and HIPP balances the decrease of circulating Oxt.

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Galanin gene expression in radiothyroidectomy-induced thyrotroph adenomas

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1996.271.1.e24 ◽

1996 ◽

Vol 271 (1) ◽

pp. E24-E30

Author(s):

J. F. Hyde ◽

J. P. Moore ◽

K. W. Drake ◽

D. G. Morrison

Keyword(s):

Gene Expression ◽

Thyroid Hormones ◽

Anterior Pituitary ◽

Human Growth ◽

Mrna Levels ◽

Male Mice ◽

Pituitary Glands ◽

Positive Regulators ◽

Hypothalamic Pituitary Axis ◽

Iodine 131

Galanin gene expression is markedly increased in the anterior pituitary glands of estrogen-treated rats (lactotroph hyperplasia) as well as human growth hormone-releasing hormone transgenic mice (somatotroph hyperplasia). The objective of this study was to examine galanin in a mouse model of thyrotroph adenoma formation. Male mice were radiothyroidectomized by use of iodine-131 (131I), and galanin peptide levels were assessed in the hypothalamic-pituitary axis. Immunoreactive galanin concentrations in the anterior pituitaries of 131I-treated mice were decreased 80% at 3, 6, 9, and 12 mo after radiothyroidectomy. Galanin peptide levels in the hypothalamus were decreased 20-25% at these times. Treatment with either estradiol or 3,3',5-triiodo-L-thyronine increased galanin peptide concentrations in the anterior pituitaries of 131I-treated mice, but neither treatment restored galanin concentrations. Galanin mRNA levels were decreased > 80% 1 yr after radiothyroidectomy. We conclude that, unlike animal models of lactotroph and somatotroph hyperplasia, galanin gene expression is suppressed throughout the development of thyrotroph adenomas, suggesting that galanin does not have a stimulatory role in the proliferation of thyrotrophs. Moreover, these data show that thyroid hormones are important positive regulators of galanin gene expression in the mouse and that estrogen may stimulate galanin gene expression in the absence of thyroid hormones.

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Exercise induces region‐specific remodeling of astrocyte morphology and reactive astrocyte gene expression patterns in male mice

Journal of Neuroscience Research ◽

10.1002/jnr.24430 ◽

2019 ◽

Vol 97 (9) ◽

pp. 1081-1094 ◽

Cited By ~ 5

Author(s):

Adam J. Lundquist ◽

Jacqueline Parizher ◽

Giselle M. Petzinger ◽

Michael W. Jakowec

Keyword(s):

Gene Expression ◽

Expression Patterns ◽

Reactive Astrocyte ◽

Gene Expression Patterns ◽

Male Mice

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Analysis of β-catenin gene mutations and gene expression in liver tumours of C57BL/10J mice produced by chronic administration of sodium phenobarbital

Toxicology ◽

10.1016/j.tox.2019.152343 ◽

2020 ◽

Vol 430 ◽

pp. 152343 ◽

Cited By ~ 2

Author(s):

James E. Sidaway ◽

Terry C. Orton ◽

Kassiani Kalaitzi ◽

Huw B. Jones ◽

Alison Foster ◽

...

Keyword(s):

Gene Expression ◽

Gene Mutations ◽

Chronic Administration ◽

Liver Tumours ◽

Sodium Phenobarbital

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Dysfunction in Ribosomal Gene Expression in the Hypothalamus and Hippocampus following Chronic Social Defeat Stress in Male Mice as Revealed by RNA-Seq

Neural Plasticity ◽

10.1155/2016/3289187 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 22

Author(s):

Dmitry A. Smagin ◽

Irina L. Kovalenko ◽

Anna G. Galyamina ◽

Anatoly O. Bragin ◽

Yuriy L. Orlov ◽

...

Keyword(s):

Gene Expression ◽

Social Stress ◽

Ribosome Biogenesis ◽

Social Defeat ◽

Brain Regions ◽

Ribosomal Gene ◽

Rna Seq ◽

Male Mice ◽

Social Defeat Stress ◽

Chronic Social Defeat Stress

Chronic social defeat stress leads to the development of anxiety- and depression-like states in male mice and is accompanied by numerous molecular changes in brain. The influence of 21-day period of social stress on ribosomal gene expression in five brain regions was studied using the RNA-Seq database. MostRps, Rpl, Mprs, andMprlgenes were upregulated in the hypothalamus and downregulated in the hippocampus, which may indicate ribosomal dysfunction following chronic social defeat stress. There were no differentially expressed ribosomal genes in the ventral tegmental area, midbrain raphe nuclei, or striatum. This approach may be used to identify a pharmacological treatment of ribosome biogenesis abnormalities in the brain of patients with “ribosomopathies.”

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