scholarly journals Identification and Integrated Analysis of MicroRNA and mRNA Expression Profiles During Agonistic Behavior in Chinese Mitten Crab (Eriocheir sinensis) Using a Deep Sequencing Approach

2020 ◽  
Vol 11 ◽  
Author(s):  
Yangyang Pang ◽  
Long He ◽  
Yameng Song ◽  
Xiaozhe Song ◽  
Jiahuan Lv ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Deep Sequencing ◽  
Agonistic Behavior ◽  
Expression Profiles ◽  
Eriocheir Sinensis ◽  
Integrated Analysis ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Deep Sequencing Approach

scholarly journals Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yang-Yang Pang ◽  
Gen-Yong Huang ◽  
Ya-Meng Song ◽  
Xiao- Zhe Song ◽  
Jia-Huan Lv ◽  
...  
Keyword(s):  
Agonistic Behavior ◽  
Cost Effective ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Physical Damage ◽  
Phylogenetic Tree Analysis ◽  
Important Species ◽  
Downstream Target ◽  
Mitten Crab

AbstractChinese mitten crab (Eriocheir sinensis) as a commercially important species is widely cultured in China. However, E. sinensis is prone to agonistic behavior, which causes physical damage and wastes energy resources, negatively impacting their growth and survival. Therefore, understanding the regulatory mechanisms that underlie the switching of such behavior is essential for ensuring the efficient and cost-effective aquaculture of E. sinensis. The 5-HT2B receptor is a key downstream target of serotonin (5-HT), which is involved in regulating animal behavior. In this study, the full-length sequence of 5-HT2B gene was cloned. The total length of the 5-HT2B gene was found to be 3127 bp with a 236 bp 5′-UTR (untranslated region), a 779 bp 3′-UTR, and a 2112 bp open reading frame encoding 703 amino acids. Phylogenetic tree analysis revealed that the 5-HT2B amino acid sequence of E. sinensis is highly conserved with that of Cancer borealis. Using in vitro co-culture and luciferase assays, the miR-143 targets the 5-HT2B 3′-UTR and inhibits 5-HT2B expression was confirmed. Furthermore, RT-qPCR and Western blotting analyses revealed that the miR-143 mimic significantly inhibits 5-HT2B mRNA and protein expression. However, injection of miR-143 did not decrease agonistic behavior, indicating that 5-HT2B is not involved in the regulation of such behavior in E. sinensis.

A clip domain serine protease (cSP) from the Chinese mitten crab Eriocheir sinensis: cDNA characterization and mRNA expression

2009 ◽  
Vol 27 (6) ◽  
pp. 670-677 ◽  
Author(s):  
Yunchao Gai ◽  
Limei Qiu ◽  
Lingling Wang ◽  
Linsheng Song ◽  
Changkao Mu ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Serine Protease ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab

scholarly journals Tissue-expression profiles unveils the gene interaction of hepatopancreas, eyestalk, and ovary in precocious female Chinese mitten crab, Eriocheir sinensis

2018 ◽  
Author(s):  
Xiaowen Chen ◽  
Jun Wang ◽  
Xin Hou ◽  
Wucheng Yue ◽  
Shu Huang ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Expression Profiles ◽  
Tissue Expression ◽  
Eriocheir Sinensis ◽  
Lipid Transport ◽  
Vitelline Membrane ◽  
Ovary Development ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Neuropeptide Hormone

AbstractSexual precocity is a serious and common biological phenomenon in animal species. Large amount of precocity individuals was identified in Chinese mitten crab, Eriocheir sinensis, which caused huge economical loss every year. However, the underlying genetic basis of precocity in E. sinensis is still lack. In this study, histology observation, comparative transcriptome was conducted among different stages of precocious one-year old and normal two-year old E. sinensis, tissue-expression profiles of ovary, hepatopancreas, and eyestalk tissues were presented and compared. Genes associated with lipid metabolic process, lipid transport, vitelline membrane formation, vitelline synthesis and neuropeptide hormone related genes were upregulated in the ovary, hepatopancreas and eyestalk of precocious E. sinensis. Our results indicated eyestalk involved in neuroendocrine system providing neuropeptide hormone that may induce vitellogenesis in hepatopancreas and further stimulate ovary development. Hepatopancreas is a site for energy storage, vitellogenin synthesis and may assist to induce oogenesis through lipid transport in precocious E. sinensis. The genetic basis of precocity in E. sinensis is an integrated gene regulatory network of eyestalk, hepatopancreas, and ovary tissues. Our study provides effective convenient phenotype measurement method for identification of potential precocious E. sinensis detection, and valuable genetic resources and novel insights into the research of molecular mechanism of precocity in E. sinensis.

cDNA cloning, characterization and mRNA expression of cryptocyanin from the Chinese mitten crab, Eriocheir sinensis H. Milne Edwards, 1853

Crustaceana ◽  
2016 ◽  
Vol 89 (3) ◽  
pp. 273-290 ◽  
Author(s):  
Xingqiang Wang ◽  
Lingling Wang ◽  
Mengqiang Wang ◽  
Huan Zhang ◽  
Linsheng Song
Keyword(s):  
Untranslated Region ◽  
Expression Profiles ◽  
Vibrio Anguillarum ◽  
Eriocheir Sinensis ◽  
Amino Acid Residues ◽  
Chinese Mitten Crab ◽  
Reading Frame ◽  
Mitten Crab ◽  
Inflammatory Processes ◽  
Moulting Cycle

Cryptocyanin is a copper-free non-respiratory protein and plays an important role in the physiological process of decapod crustaceans. In the present study, the full length cDNA encoding cryptocyanin (EsCc) was cloned from the haemocytes of the Chinese mitten crab Eriocheir sinensis, which was 2630 bp, consisting of a 5′-untranslated region of 237 bp, a 3′-untranslated region of 359 bp, and an open reading frame of 2034 bp. The deduced protein possessed 678 amino-acid residues, and the predicted molecular mass was 79.09 kDa. The phylogenetic analysis revealed that it belonged to the arthropod haemocyanin superfamily and shared 78% homology to Dungeness crab Metacarcinus magister cryptocyanin subunit 2. The expression pattern of EsCc in five tissues was investigated in premoult, intermoult and postmoult stages to understand its role during the moulting cycle. In hepatopancreas, the significant up-regulation of EsCc expression was observed in both premoult and intermoult stages, while the significant change of EsCc expressions in muscle and haemocytes was only found in premoult stages. To gain insight into the antimicrobial activity of EsCc in inflammatory processes, its expression profiles in the haemolymph of Chinese mitten crab challenged with Vibrio anguillarum and Pichia pastoris were examined. The challenge of V. anguillarum induced higher expression of EsCc as early as 6 h post-infection. Instead, a significant up-regulation of EsCc transcript was observed at 12 h post P. pastoris challenge. The above results implied that crustacean cryptocyanin was involved in the new exoskeleton formation. Also, it might be induced to resist invasions of pathogens.

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