Transcriptional Control of Quality Differences in the Lipid-Based Cuticle Barrier in Drosophila suzukii and Drosophila melanogaster

Abstract Background Drosophila suzukii is widely distributed. Research has revealed that the presence of Drosophila melanogaster can reduce the emergence and egg laying of D. suzukii. However, the reasons for these phenomena have not yet been reported. To investigate this issue, we sought to answer three questions: Can the presence of D. melanogaster reduce the longevity of D. suzukii? Does D. melanogaster dominate in larval interspecific competition with D. suzukii? Does reproductive interference occur between these species; i.e., do individuals of one species (e.g., D. suzukii) engage in reproductive activities with individuals of the other (e.g., D. melanogaster) such that the fitness of one or both species is reduced? Results The results showed that the adult offspring number of Drosophila suzukii was significantly reduced when this species was reared with Drosophila melanogaster. The larval interspecific competition had no significant effects on Drosophila suzukii longevity or population size. Surprisingly, Drosophila melanogaster imposed reproductive interference on males of Drosophila suzukii, which led to a significant decline in the rate of successful mating of the latter species. Conclusions The presence of Drosophila melanogaster causes the population size of Drosophila suzukii to decrease through reproductive interference, and the rate of successful mating in Drosophila suzukii is significantly reduced in the presence of Drosophila melanogaster.

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Peer Review #3 of "The microbiota of Drosophila suzukii influences the larval development of Drosophila melanogaster (v0.1)"

10.7287/peerj.8097v0.1/reviews/3 ◽

2019 ◽

Author(s):

S Fellous

Keyword(s):

Drosophila Melanogaster ◽

Larval Development ◽

Peer Review ◽

Drosophila Suzukii

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Polycomb Assemblies Multitask to Regulate Transcription

Epigenomes ◽

10.3390/epigenomes3020012 ◽

2019 ◽

Vol 3 (2) ◽

pp. 12 ◽

Cited By ~ 2

Author(s):

Miguel Vidal

Keyword(s):

Gene Expression ◽

Drosophila Melanogaster ◽

Transcriptional Control ◽

Catalytic Properties ◽

Integrated Approach ◽

Gene Repression ◽

Developmental Studies ◽

Topological Domains ◽

Developmental Transitions ◽

New Findings

The Polycomb system is made of an evolutionary ancient group of proteins, present throughout plants and animals. Known initially from developmental studies with the fly Drosophila melanogaster, they were associated with stable sustainment of gene repression and maintenance of cell identity. Acting as multiprotein assemblies with an ability to modify chromatin, through chemical additions to histones and organization of topological domains, they have been involved subsequently in control of developmental transitions and in cell homeostasis. Recent work has unveiled an association of Polycomb components with transcriptionally active loci and the promotion of gene expression, in clear contrast with conventional recognition as repressors. Focusing on mammalian models, I review here advances concerning roles in transcriptional control. Among new findings highlighted is the regulation of their catalytic properties, recruiting to targets, and activities in chromatin organization and compartmentalization. The need for a more integrated approach to the study of the Polycomb system, given its fundamental complexity and its adaptation to cell context, is discussed.

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Invasive Drosophila suzukii facilitates Drosophila melanogaster infestation and sour rot outbreaks in the vineyards

Royal Society Open Science ◽

10.1098/rsos.170117 ◽

2017 ◽

Vol 4 (3) ◽

pp. 170117 ◽

Cited By ~ 28

Author(s):

A. Rombaut ◽

R. Guilhot ◽

A. Xuéreb ◽

L. Benoit ◽

M. P. Chapuis ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Disease Outbreaks ◽

Invasive Pest ◽

Drosophila Suzukii ◽

Extensive Disease ◽

Field Collection ◽

Invasive Pests ◽

Sour Rot ◽

Independent Assay ◽

Grapevine Disease

How do invasive pests affect interactions between members of pre-existing agrosystems? The invasive pest Drosophila suzukii is suspected to be involved in the aetiology of sour rot, a grapevine disease that otherwise develops following Drosophila melanogaster infestation of wounded berries. We combined field observations with laboratory assays to disentangle the relative roles of both Drosophila in disease development. We observed the emergence of numerous D. suzukii , but no D. melanogaster flies, from bunches that started showing mild sour rot symptoms days after field collection. However, bunches that already showed severe rot symptoms in the field mostly contained D. melanogaster . In the laboratory, oviposition by D. suzukii triggered sour rot development. An independent assay showed the disease increased grape attractiveness to ovipositing D. melanogaster females. Our results suggest that in invaded vineyards, D. suzukii facilitates D. melanogaster infestation and, consequently, favours sour rot outbreaks. Rather than competing with close species, the invader subsequently permits their reproduction in otherwise non-accessible resources and may cause more frequent, or more extensive, disease outbreaks.

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Transformation of cultured Drosophila melanogaster cells with a dominant selectable marker.

Molecular and Cellular Biology ◽

10.1128/mcb.5.8.1833 ◽

1985 ◽

Vol 5 (8) ◽

pp. 1833-1838 ◽

Cited By ~ 66

Author(s):

D C Rio ◽

G M Rubin

Keyword(s):

Drosophila Melanogaster ◽

Transposable Element ◽

Transcriptional Control ◽

Fusion Gene ◽

Plasmid Vector ◽

Resistant Cell ◽

Resistant Cell Line ◽

Culture Cells ◽

Dominant Selectable Marker ◽

Neomycin Resistance

We have developed a method for the stable and efficient introduction of foreign DNA into Drosophila melanogaster tissue culture cells. A plasmid vector was constructed that carries the bacterial neomycin resistance gene under the transcriptional control of the copia transposable element long terminal repeat promoter. After calcium phosphate-DNA transfection, this vector rendered D. melanogaster cells resistant to the aminoglycoside G-418, a derivative of gentamicin. The vector DNA appeared to be integrated in long tandem arrays of 10 to 20 copies per cell and was stable for many generations in the absence of selection. To test the usefulness of this system for introducing nonselected DNA into D. melanogaster cells, a gene fusion between the P transposable element and the hsp70 promoter was inserted into the copia-neomycin resistance plasmid. After transfection and establishment of a G-418-resistant cell line, the hsp-P fusion gene was found to be efficiently transcribed after heat shock.

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Microbiota-mediated competition between pest insects and its application to the evolution-proof protection of crops

10.1101/2020.08.05.238055 ◽

2020 ◽

Author(s):

Antoine Rombaut ◽

Romain Gallet ◽

Kenza Qitout ◽

Mukherjy Samy ◽

Robin Guilhot ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Ecological Niche ◽

Insect Pest ◽

Adaptive Significance ◽

Condition Dependence ◽

Larval Competition ◽

Drosophila Suzukii ◽

Pest Insects ◽

Competition Avoidance ◽

Over Time

AbstractSpecies that share the same resources often avoid costly competition with contextdependent behaviors. This is the case of the invasive insect pest Drosophila suzukii which larvae’s ecological niche overlaps with that of Drosophila melanogaster in ripe, but not rotten, fruit. Previous research showed D. suzukii prevents costly larval competition by not ovipositing in substrates with D. melanogaster eggs. We discovered D. suzukii females respond to cues produced by D. melanogaster adults associated to gut microbiota bacteria. This behavior of D. suzukii varied over time and among populations, revealing subtle condition-dependence. In particular, D. suzukii females that bore D. melanogaster gut bacteria stopped avoiding sites with D. melanogaster cues. The adaptive significance of the behavior was investigated by reproducing experimentally in-fruit larval competition. D. suzukii larvae only suffered from competition with D. melanogaster if the competitor was associated to its microbiota, suggesting D. suzukii has evolved a solution to avoid its offspring develops in challenging environments. We argue that D. suzukii’s competition avoidance behavior has features enabling the design of an evolution-proof repellent to protect crops.

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Phenotypic rescue by a bovine transgene in a Cu/Zn superoxide dismutase-null mutant of Drosophila melanogaster

Molecular and Cellular Biology ◽

10.1128/mcb.14.2.1302-1307.1994 ◽

1994 ◽

Vol 14 (2) ◽

pp. 1302-1307

Author(s):

I Reveillaud ◽

J Phillips ◽

B Duyf ◽

A Hilliker ◽

A Kongpachith ◽

...

Keyword(s):

Superoxide Dismutase ◽

Drosophila Melanogaster ◽

Life Span ◽

Male Fertility ◽

Transcriptional Control ◽

Adult Life ◽

P Element ◽

Normal Male ◽

Null Mutant ◽

Adult Life Span

Null mutants for Cu/Zn superoxide dismutase (CuZnSOD) in Drosophila melanogaster are male sterile, have a greatly reduced adult life span, and are hypersensitive to paraquat. We have introduced a synthetic bovine CuZnSOD transgene under the transcriptional control of the D. melanogaster 5C actin promoter into a CuZnSOD-null mutant of D. melanogaster. This was carried out by P-element-mediated transformation of the Drosophila-bovine CuZnSOD transgene into a CuZnSOD+ recipient strain followed by genetic crossing of the transgene into a strain carrying the CuZnSOD-null mutation, cSODn108. The resulting transformants express bovine CuZnSOD exclusively to about 30% of normal Drosophila CuZnSOD levels. Expression of the Drosophila-bovine CuZnSOD transgene in the CuZnSOD-null mutant rescues male fertility and resistance to paraquat to apparently normal levels. However, adult life span is restored to only 30% of normal, and resistance to hyperoxia is 90% of that found in control flies. This striking differential restoration of pleiotropic phenotypes could be the result of a threshhold of CuZnSOD expression necessary for normal male fertility and resistance to the toxicity of paraquat or hyperoxia which is lower than the threshold required to sustain a normal adult life span. Alternatively, the differential rescue of fertility, resistance to active oxygen, and life span might indicate different cell-specific transcriptional requirements for these functions which are normally provided by the control elements of the native CuZnSOD gene but are only partly compensated for by the transcriptional control elements of the actin 5C promoter.

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