scholarly journals Pre-Existing Humoral Immunity Enhances Epicutaneously-Administered Allergen Capture by Skin DC and Their Migration to Local Lymph Nodes

2021 ◽  
Vol 12 ◽  
Author(s):  
Pierre-Louis Hervé ◽  
Camille Plaquet ◽  
Noémie Assoun ◽  
Nathalie Oreal ◽  
Laetitia Gaulme ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Humoral Immunity ◽  
Blocking Antibody ◽  
Patch Application ◽  
Skin Delivery ◽  
Specific Igg ◽  
Antigen Presenting ◽  
Draining Lymph Nodes ◽  
Specific Igg Antibodies

Due to its richness in antigen presenting cells, e.g., dendritic cells (DC), the skin has been identified as a promising route for immunotherapy and vaccination. Several years ago, a skin delivery system was developed based on epicutaneous patches allowing the administration of antigen through intact skin. Using mouse models, we have shown that epicutaneous allergen application leads to a rapid uptake and transport of allergen-positive cells to skin-draining lymph nodes (LN). This occurred primarily in animals previously sensitized to the same allergen. In that context, we sought to better understand the role of the specific preexisting immunity in allergen capture by skin DC and their subsequent migration to LN. Specifically, we investigated the role of humoral immunity induced by sensitization and the involvement of IgG Fc receptors (FcγR). Epicutaneous patches containing fluorescently-labeled ovalbumin (OVA) were applied to naïve mice that had previously received either sera or purified IgG isolated from OVA-sensitized mice. To investigate the involvement of FcγR, animals received 2.4G2 (anti-FcγRII/RIII) blocking antibody, 24 hours before patch application. Mice that received sera or purified IgG originating from OVA-sensitized mice showed an increase in the quantity of OVA-positive DC in skin and LN. Moreover, the blockade of FcγR reduced the number of OVA-positive DC in LN to a level similar to that observed in naïve animals. Overall, these results demonstrate that preexisting specific-IgG antibodies are involved in allergen capture by skin DC following EPIT through the involvement of antigen-specific IgG-FcγR.

scholarly journals Inhalation of Ortho-Phthalaldehyde Vapor Causes Respiratory Sensitization in Mice

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Victor J. Johnson ◽  
Jeffrey S. Reynolds ◽  
Wei Wang ◽  
Kara Fluharty ◽  
Berran Yucesoy
Keyword(s):  
Lymph Nodes ◽  
B Lymphocytes ◽  
Case Reports ◽  
Inhalation Exposure ◽  
Cytokine Gene Expression ◽  
Respiratory Problems ◽  
Specific Igg ◽  
High Level ◽  
Lymphocyte Populations ◽  
Draining Lymph Nodes

Ortho-Phthalaldehyde (OPA) has been approved for high-level sterilization of heat-sensitive medical instruments and is increasingly being used as a replacement in the healthcare industry for glutaraldehyde, a known sensitizer. Numerous case reports have been published indicating workers and patients experiencing respiratory problems, anaphylaxis, skin reactivity, and systemic antibody production. Our laboratory previously demonstrated that OPA is a dermal sensitizer in mice. The goal of the present study was to determine if OPA is a respiratory sensitizer following inhalation exposure. Mice were exposed to OPA vapor and airway and lymph nodes were examined for cytokine gene expression and alterations in lymphocyte populations. Inhalation of OPA for 3 days resulted in a concentration-dependent increase in lymphocyte proliferation, mainly B lymphocytes, in the draining lymph nodes. A secondary challenge of mice with OPA resulted in a dramatic increase in the population of B lymphocytes expressing IgE. Expression of Th2 (IL-4, IL-5, and IL-13) and anti/proinflammatory (IL-10, TNFα, and IL-1β) cytokine genes was upregulated in the lymph nodes and the nasal mucosa. Mice exposed to the higher concentrations of OPA-produced OPA-specific IgG1 antibodies indicating systemic sensitization. These findings provide evidence that OPA has the potential to cause respiratory sensitization in mice.

scholarly journals Pathogenic role of glycan-specific IgG antibodies in IgA nephropathy

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Yan-feng Zhao ◽  
Li Zhu ◽  
Li-jun Liu ◽  
Su-fang Shi ◽  
Ji-cheng Lv ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Igg Antibodies ◽  
Pathogenic Role ◽  
Specific Igg ◽  
Specific Igg Antibodies

Cellular infiltration at skin lesions and draining lymph nodes of sheep infested with adultHyalomma anatolicum anatolicumticks

Parasitology ◽  
2005 ◽  
Vol 131 (5) ◽  
pp. 657-667 ◽  
Author(s):  
D. K. V. BOPPANA ◽  
S. K. WIKEL ◽  
D. G. RAJ ◽  
M. B. MANOHAR ◽  
J. LALITHA
Keyword(s):  
T Cells ◽  
Lymph Nodes ◽  
Antigen Presenting Cells ◽  
Skin Lesions ◽  
Cellular Infiltration ◽  
Regional Lymph Nodes ◽  
Mhc Ii ◽  
Skin Biopsies ◽  
Antigen Presenting ◽  
Draining Lymph Nodes

Immunohistochemical analysis of skin and draining lymph nodes of sheep repeatedly infested with the ixodid tickHyalomma anatolicum anatolicumwere studied for different antigen-presenting cells and lymphocyte subpopulations. Infiltration of neutrophils, macrophages and lymphocytes adjacent to the tick bite site were observed. Skin biopsies showed significant increases in dermal infiltration of CD8+and γδ+T cells at 72 h and 8 days after both primary and secondary infestation. Infiltrations of MHC-II DR/DQ decreased at 72 h after tick infestation, whereas significant increases were recorded for 8-day skin biopsies. CD1+cellular infiltrations were observed during secondary infestations at the dermis. Decreased ratios of CD4[ratio ]CD8 T cells and MHC-II[ratio ]CD1 antigen-presenting cells were observed in both infestations compared to healthy skin biopsies. Ratios of αβ[ratio ]γδ T cells increased gradually during infestation compared to uninfested skin. The regional lymph nodes from tick-infested sheep showed an increased CD8+, γδ+T and CD1+cellular infiltration compared to control lymph nodes. CD4+T cells were decreased. There were no significant changes in CD45R+cellular infiltration either at skin lesions or regional lymph nodes.

scholarly journals Occurrence of COVID-19 Symptoms During SARS-CoV-2 Infection Defines Waning of Humoral Immunity

2021 ◽  
Vol 12 ◽  
Author(s):  
Jun Wu ◽  
Bo-Yun Liang ◽  
Yao-Hui Fang ◽  
Hua Wang ◽  
Xiao-Li Yang ◽  
...  
Keyword(s):  
Humoral Immunity ◽  
Antibody Responses ◽  
Surveillance Program ◽  
Igg Antibodies ◽  
Immunization Programs ◽  
Specific Igg ◽  
Asymptomatic Infections ◽  
Asymptomatic Individuals ◽  
Specific Igg Antibodies

Approximately half of the SARS-CoV-2 infections occur without apparent symptoms, raising questions regarding long-term humoral immunity in asymptomatic individuals. Plasma levels of immunoglobulin G (IgG) and M (IgM) against the viral spike or nucleoprotein were determined for 25,091 individuals enrolled in a surveillance program in Wuhan, China. We compared 405 asymptomatic individuals who mounted a detectable antibody response with 459 symptomatic COVID-19 patients. The well-defined duration of the SARS-CoV-2 endemic in Wuhan allowed a side-by-side comparison of antibody responses following symptomatic and asymptomatic infections without subsequent antigen re-exposure. IgM responses rapidly declined in both groups. However, both the prevalence and durability of IgG responses and neutralizing capacities correlated positively with symptoms. Regardless of sex, age, and body weight, asymptomatic individuals lost their SARS-CoV-2-specific IgG antibodies more often and rapidly than symptomatic patients did. These findings have important implications for immunity and favour immunization programs including individuals after asymptomatic infections.

Antigen-presenting cells in draining lymph nodes of goats repeatedly infested by the Cayenne tick Amblyomma cajennense nymphs

2010 ◽  
Vol 53 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Gaby Ermelindo Roberto Monteiro ◽  
Gervásio Henrique Bechara ◽  
Alessandra Maria Franzin ◽  
Isabel Kinney Ferreira de Miranda Santos
Keyword(s):  
Lymph Nodes ◽  
Antigen Presenting Cells ◽  
Amblyomma Cajennense ◽  
Antigen Presenting ◽  
Draining Lymph Nodes

scholarly journals Capture of influenza by medullary dendritic cells via SIGN-R1 is essential for humoral immunity in draining lymph nodes

2010 ◽  
Vol 11 (5) ◽  
pp. 427-434 ◽  
Author(s):  
Santiago F Gonzalez ◽  
Veronika Lukacs-Kornek ◽  
Michael P Kuligowski ◽  
Lisa A Pitcher ◽  
Søren E Degn ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Lymph Nodes ◽  
Humoral Immunity ◽  
Draining Lymph Nodes

Experimental Echinococcus granulosus infection in mice: immunocytochemical analysis of lymphocyte populations in local lymphoid infections during early infection

Parasitology ◽  
1987 ◽  
Vol 94 (3) ◽  
pp. 523-532 ◽  
Author(s):  
Eleanor M. Riley ◽  
J. B. Dixon
Keyword(s):  
Lymph Nodes ◽  
Echinococcus Granulosus ◽  
Post Infection ◽  
Potential Role ◽  
Lymphocyte Subsets ◽  
Suppressor Cells ◽  
Lymphocyte Populations ◽  
Draining Lymph Nodes ◽  
T Suppressor Cells

The influence of subcutaneous infection with protoscoleces of Echinococcus granulosus on the distribution of lymphocyte subsets in draining lymph nodes has been evaluated by immunocytochemical labelling of lymphocyte surface antigens. These studies reveal marked expansion of paracortical Thy-1 +, Lyt-1 + cells and moderate proliferation of surface immunoglobulin-bearing B-cells immediately after infection. The Lyt-1 +:Lyt-2 + ratio decreases rapidly during the first 21 days post-infection and remains below unity until at least 12 weeks post-infection due to severe depletion of Lyt-1 + cells in draining lymph nodes and a significant increase in the percentage of Lyt-2 + cells. The potential role of these Lyt-1 ∓, 2 + (putative T-suppressor) cells in regulation of the anti-parasite immune response and mediation of generalized immunosuppression is discussed in the light of evidence of inhibition of anti-parasite immunity in infected mice.

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