scholarly journals Wound Vitality in Decomposed Bodies: New Frontiers Through Immunohistochemistry

2021 ◽  
Vol 8 ◽  
Author(s):  
Giuseppe Bertozzi ◽  
Michela Ferrara ◽  
Raffaele La Russa ◽  
Giovanni Pollice ◽  
Giovanni Gurgoglione ◽  
...  
Keyword(s):  
Qualitative Method ◽  
Soft Tissues ◽  
Immunohistochemical Study ◽  
Matrix Proteins ◽  
Forensic Sciences ◽  
Wound Age ◽  
Limiting Factor ◽  
Glycophorin A ◽  
Experimental Application ◽  
Combined Use

Background: The question about wound vitality and the estimation of wound age of production are two of the classic investigation fields of forensic sciences. To answer this, the techniques most frequently used in research studies are immunohistochemistry (IHC), molecular biology, and biochemistry. Despite the great data on the literature about the usefulness of IHC in forensic pathology, there is always a request for further studies, especially on tissues altered by putrefactive phenomena. In fact, the degradation of the tissues is intended as the main limiting factor to the use of this technique.Scope: The aim of this pilot study was to evaluate the immunohistochemical behavior of samples collected from decomposed bodies (in different putrefaction phases) and to relate these findings to wound vitality and postmortem interval.Materials and Methods: Samples of skin and soft tissues were collected during autopsies, which were executed on decomposed bodies, whose cause of death was concluded to be traumatic. An immunohistochemical study was performed using antibodies against CD15, CD45, IL-15, tryptase, and glycophorin-A MMPs (endopeptidases involved in degrading extracellular matrix proteins: MMP-9 and MMP-2). An immunohistochemistry (IHC) reaction was evaluated according to a qualitative method as the following legend: (0): not expressed, (+): isolated and disseminated expression, (++): expression in groups or widespread foci, and (+++): widespread expression.Results: Most of the tested markers (tryptase, glycophorin, IL15, CD 15, CD 45, and MMP9) showed to be highly expressed in the tissue of putrefied skin for 15 days.Discussion and Conclusion: Although certainly inconclusive, this experimental application demonstrated that a nonexclusive but combined use of multiple antibodies is appropriate to verify wound vitality in decomposed bodies. Among them, GPA exhibited major reliability.

scholarly journals Immunohistochemical Study Of Fibronectin, Tenascin and Type I Collagen In Tongue And Lip carcinomas

2017 ◽  
Vol 16 ◽  
pp. 1-13
Author(s):  
João Luiz de Miranda ◽  
Felipe Rodrigues de Matos ◽  
Frederico Santos Lages ◽  
Dhelfeson Willya Douglas-de-Oliveira ◽  
Roseana de Almeida Freitas
Keyword(s):  
Type I Collagen ◽  
Immunohistochemical Study ◽  
Immunohistochemical Analysis ◽  
Tumor Stroma ◽  
Matrix Proteins ◽  
Type I ◽  
Lower Lip ◽  
Strong Expression ◽  
Weak Expression ◽  
Malignant Grade

AIM: The aim was to compare the immunoexpression of extracellular matrix proteins in squamous cell carcinomas of tongue (SCCTo) and lower lip (SCCLi). METHODS: Eleven SCCTo and 11 SCCLi were selected and examined according to Bryne’s method (1998). For immunohistochemical study utilized antibodies to fibronectin, tenascin and type I collagen. Histopathologic and immunohistochemical analysis were performed on the tumor invasive front. RESULTS: All SCCTo were classified in high score malignant grade and all SCCLi in lower score. Fibronectin showed strong immunorreactivity in the peritumoral basement membrane (BM) in 91% of SCCTo and all cases of SCCLi, while in the tumor stroma (TS) all cases of SCCTo and SCCLi had strong intensity. Tenascin had strong expression in BM of 91% cases of SCCTo and 63.4% of SCCLi and in TS had strong expression in 91% cases of SCCTo and 54.6% of SCCLi. Type I collagen demonstrated weak immunoreactivity in the TS of 72.7% cases of SCCTo and 63.4% of SCCLi. CONCLUSION: These results may suggest that the strong expression of fibronectin and tenascin proteins and the weak expression of type I collagen could play a role in the invasive process of oral SCC.

Potassium metabolism and the accumulation of 137Caesium by decapod Crustacea

1962 ◽  
Vol 42 (2) ◽  
pp. 199-241 ◽  
Author(s):  
G. W. Bryan ◽  
Eileen Ward
Keyword(s):  
Body Surface ◽  
Soft Tissues ◽  
Sea Water ◽  
The Body ◽  
Limiting Factor ◽  
Freshwater Crayfish ◽  
Marine Animals ◽  
Decapod Crustacea ◽  
Concentration Factors ◽  
Potassium Metabolism

SUMMARYThe accumulation of 137Cs from sea water has been examined in relation to potassium metabolism in the lobster Homarus vulgaris and in the prawn Palaemon serratus. In unfed animals 137Cs is taken up and lost far more slowly than 42K. Although all the inactive K in the animals can be exchanged with 42K, higher whole-animal concentration factors are reached for 137Cs (about eight for lobsters and twenty-five for prawns). This is because both species have higher plasma/medium ratios for 137Cs than K at equilibrium despite the selective excretion of 137Cs. Also, except for the hepatopancreas in lobsters and fed prawns, all soft tissues can probably attain higher tissue/plasma ratios for 137Cs than inactive K.Uptake of both isotopes has also been studied in the freshwater crayfish Austropotamobius pallipes pallipes. In crayfish in o-i % sea water 137Cs is not concentrated to the same extent as K by whole animals (50-200 for 137Cs against about 4500 for K). Although the situation between plasma and tissues resembles that in the marine animals, 137Cs cannot be accumulated in the plasma to the same degree as K. Crayfish selectively excrete 137Cs in the urine relative to K at a lower concentration than in the plasma.In the accumulation of 137Cs by all species, muscle is the principal limiting factor in uptake and loss, but with 42K the body surface becomes more limiting.Experiments on the absorption of 137Cs from food in prawns and freshwater crayfish have been carried out. In prawns in a constant environment, feeding is probably less important than uptake over the body surface while in crayfish feeding is probably much more important.

Flow Cytometry, Beads, and Microchannels

2005 ◽  
Author(s):  
Tione Buranda ◽  
Larry A. Sklar
Keyword(s):  
Flow Cytometry ◽  
Dna Analysis ◽  
Microfluidic Devices ◽  
Model Systems ◽  
Limiting Factor ◽  
Flat Surfaces ◽  
Combined Use ◽  
Potential Applications ◽  
Small Reynolds Numbers ◽  
Flow Through

Microfluidic devices generally consume microliter to submicroliter volumes of sample and are thus well suited for use when the required reagents are scarce or expensive. Because microfluidic devices operate in a regime in which small Reynolds numbers govern the delivery of fluid samples, reagent mixing and subsequent reactivity has been a severe limiting factor in their applicability. The inclusion of packed beads in the microfluidic device repertoire has several advantages: molecular assemblies for the assay are created outside the channel on beads and characterized with flow cytometry, uniform populations of beads may be assured through rapid cytometric sorting, beads present a larger surface area for the display of receptors than flat surfaces, rapid mixing in the microcolumn is achieved because the distance that must be covered by diffusion is limited to the (≤1-μm) interstitial space between the closely packed receptorbearing beads, and analytes are captured in a flow-through format and, as such, each bead can act as a local concentrator of analytes. Progress in the combined use of beads and microfluidic devices has been limited by the ability to pack beads at specific sections of microfluidic devices. A subsequent challenge associated with the packed microcolumns of beads is the substantial pressure drop that affects the fluid flow velocity. However, some of these challenges have been overcome in the design of simple model systems that have potential applications in DNA analysis, chromatography, and immunoassays. It is the intent of this chapter to examine the recent emergence of small-volume heterogeneous immunoassays, using beads trapped in microchannels, while excluding other closely related applications such as capillary electrophoresis and flow injection–based approaches. Of necessity, the authors’ interests and availability of information pertinent to the specific discussions presented below impose additional restrictions. To date, there are only a handful of applications that combine packed beads and microfluidic devices, and even fewer that make the overt connection between flow cytometry–based assays and beads. Harrison and coworkers have provided one of the earliest conceptual demonstrations of the capability to incorporate packed beads in microfluidic devices for analytical purposes.

scholarly journals An immunohistochemical study of matrix proteins in the craniofacial cartilage in midterm human fetuses

2013 ◽  
Vol 57 (4) ◽  
pp. 39 ◽  
Author(s):  
S. Shibata ◽  
Y. Sakamoto ◽  
O. Baba ◽  
C. Qin ◽  
G. Murakami ◽  
...  
Keyword(s):  
Immunohistochemical Study ◽  
Matrix Proteins ◽  
Human Fetuses

CD117 expression in peri-implantitis soft tissues: An immunohistochemical study

2010 ◽  
Vol 26 ◽  
pp. e44
Author(s):  
S.D. Aspriello ◽  
A. Zizzi ◽  
F. Bambini ◽  
M. Lorenzi ◽  
C. Rubini ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Immunohistochemical Study

Immunohistochemical study of the extracellular matrix proteins laminin, fibronectin and type IV collagen in secretory meningiomas

2008 ◽  
Vol 15 (7) ◽  
pp. 806-811 ◽  
Author(s):  
Mariella Caffo ◽  
Gerardo Caruso ◽  
Salvatore Galatioto ◽  
Francesco Meli ◽  
Fabio Cacciola ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Type Iv Collagen ◽  
Immunohistochemical Study ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Type Iv
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