scholarly journals In-cell Solid-State NMR Studies of Antimicrobial Peptides

2020 ◽  
Vol 2 ◽  
Author(s):  
Frances Separovic ◽  
David W. Keizer ◽  
Marc-Antoine Sani
Keyword(s):  
Solid State ◽  
Antimicrobial Peptides ◽  
Solid State Nmr ◽  
Bacterial Resistance ◽  
Invasive Technique ◽  
Nmr Studies ◽  
Bacterial Membranes ◽  
Modes Of Action

Antimicrobial peptides (AMPs) have attracted attention as alternatives to classic antibiotics due to their expected limited pressure on bacterial resistance mechanisms. Yet, their modes of action, in particular in vivo, remain to be elucidated. In situ atomistic-scale details of complex biomolecular assemblies is a challenging requirement for deciphering the complex modes of action of AMPs. The large diversity of molecules that modulate complex interactions limits the resolution achievable using imaging methodology. Herein, the latest advances in in-cell solid-state NMR (ssNMR) are discussed, which demonstrate the power of this non-invasive technique to provide atomic details of molecular structure and dynamics. Practical requirements for investigations of intact bacteria are discussed. An overview of recent in situ NMR investigations of the architecture and metabolism of bacteria and the effect of AMPs on various bacterial structures is presented. In-cell ssNMR revealed that the studied AMPs have a disruptive action on the molecular packing of bacterial membranes and DNA. Despite the limited number of studies, in-cell ssNMR is emerging as a powerful technique to monitor in situ the interplay between bacteria and AMPs.

A kinetic study of mechanochemical halogen bond formation by in situ31P solid-state NMR spectroscopy

2017 ◽  
Vol 53 (71) ◽  
pp. 9930-9933 ◽  
Author(s):  
Yijue Xu ◽  
Lysiane Champion ◽  
Bulat Gabidullin ◽  
David L. Bryce
Keyword(s):  
Activation Energy ◽  
Solid State ◽  
Nmr Spectroscopy ◽  
Kinetic Study ◽  
Solid State Nmr ◽  
Halogen Bond ◽  
Bond Formation ◽  
Nmr Studies ◽  
Solid State Nmr Spectroscopy

In situ 31P solid-state NMR studies of mechanochemical halogen bond formation provide insights into the cocrystallisation process and an estimate of the activation energy.

scholarly journals 1 H-Detected Solid-State NMR Studies of Water-Inaccessible Proteins In Vitro and In Situ

2016 ◽  
Vol 55 (43) ◽  
pp. 13606-13610 ◽  
Author(s):  
João Medeiros-Silva ◽  
Deni Mance ◽  
Mark Daniëls ◽  
Shehrazade Jekhmane ◽  
Klaartje Houben ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Nmr Studies

New in situ solid-state NMR techniques for probing the evolution of crystallization processes: pre-nucleation, nucleation and growth

2015 ◽  
Vol 179 ◽  
pp. 115-140 ◽  
Author(s):  
Colan E. Hughes ◽  
P. Andrew Williams ◽  
Victoria L. Keast ◽  
Vasileios G. Charalampopoulos ◽  
Gregory R. Edwards-Gau ◽  
...  
Keyword(s):  
Solid State ◽  
Liquid State ◽  
Solid State Nmr ◽  
Crystallization Process ◽  
Solid Particles ◽  
Crystal Formation ◽  
Nmr Studies ◽  
Nmr Techniques ◽  
Solid Phases

The application of in situ techniques for investigating crystallization processes promises to yield significant new insights into fundamental aspects of crystallization science. With this motivation, we recently developed a new in situ solid-state NMR technique that exploits the ability of NMR to selectively detect the solid phase in heterogeneous solid–liquid systems (of the type that exist during crystallization from solution), with the liquid phase “invisible” to the measurement. As a consequence, the technique allows the first solid particles produced during crystallization to be observed and identified, and allows the evolution of different solid phases (e.g., polymorphs) present during the crystallization process to be monitored as a function of time. This in situ solid-state NMR strategy has been demonstrated to be a powerful approach for establishing the sequence of solid phases produced during crystallization and for the discovery of new polymorphs. The most recent advance of the in situ NMR methodology has been the development of a strategy (named “CLASSIC NMR”) that allows both solid-state NMR and liquid-state NMR spectra to be measured (essentially simultaneously) during the crystallization process, yielding information on the complementary changes that occur in both the solid and liquid phases as a function of time. In this article, we present new results that highlight the application of our in situ NMR techniques to successfully unravel different aspects of crystallization processes, focusing on: (i) the application of a CLASSIC NMR approach to monitor competitive inclusion processes in solid urea inclusion compounds, (ii) exploiting liquid-state NMR to gain insights into co-crystal formation between benzoic acid and pentafluorobenzoic acid, and (iii) applications of in situ solid-state NMR for the discovery of new solid forms of trimethylphosphine oxide and l-phenylalanine. Finally, the article discusses a number of important fundamental issues relating to practical aspects, the interpretation of results and the future scope of these techniques, including: (i) an assessment of the smallest size of solid particle that can be detected in in situ solid-state NMR studies of crystallization, (ii) an appraisal of whether the rapid sample spinning required by the NMR measurement technique may actually influence or perturb the crystallization behaviour, and (iii) a discussion of factors that influence the sensitivity and time-resolution of in situ solid-state NMR experiments.

In situ solid-state NMR studies of the catalytic conversion of methanol on the molecular sieve SAPO-34

1990 ◽  
Vol 94 (7) ◽  
pp. 2730-2734 ◽  
Author(s):  
Michael W. Anderson ◽  
Bogdan. Sulikowski ◽  
Patrick J. Barrie ◽  
Jacek. Klinowski
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Molecular Sieve ◽  
Catalytic Conversion ◽  
Nmr Studies

scholarly journals Deuterium Solid State NMR Studies of Intact Bacteria Treated With Antimicrobial Peptides

2021 ◽  
Vol 2 ◽  
Author(s):  
Valerie Booth
Keyword(s):  
Solid State ◽  
Antimicrobial Peptides ◽  
Solid State Nmr ◽  
Model Membranes ◽  
Nmr Studies ◽  
Intact Cells ◽  
Whole Cells ◽  
H Nmr ◽  
Structure And Dynamics ◽  
Lipid Mixtures

Solid state NMR has been tremendously useful in characterizing the structure and dynamics of model membranes composed of simple lipid mixtures. Model lipid studies employing solid state NMR have included important work revealing how membrane bilayer structure and dynamics are affected by molecules such as antimicrobial peptides (AMPs). However, solid state NMR need not be applied only to model membranes, but can also be used with living, intact cells. NMR of whole cells holds promise for helping resolve some unsolved mysteries about how bacteria interact with AMPs. This mini-review will focus on recent studies using 2H NMR to study how treatment with AMPs affect membranes in intact bacteria.

In situ solid-state NMR studies of Ca3SiO5: hydration at room temperature and at elevated temperatures using 29Si enrichment

1994 ◽  
Vol 29 (15) ◽  
pp. 3926-3940 ◽  
Author(s):  
A. R. Brough ◽  
C. M. Dobson ◽  
I. G. Richardson ◽  
G. W. Groves
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Room Temperature ◽  
Elevated Temperatures ◽  
Nmr Studies
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