scholarly journals Simple Sample Preparation Method for Direct Microbial Identification and Susceptibility Testing From Positive Blood Cultures

2018 ◽  
Vol 9 ◽  
Author(s):  
Hong-wei Pan ◽  
Wei Li ◽  
Rong-guo Li ◽  
Yong Li ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Susceptibility Testing ◽  
Preparation Method ◽  
Blood Cultures ◽  
Sample Preparation Method ◽  
Microbial Identification ◽  
Simple Sample Preparation

scholarly journals Rapid Sepsityper in clinical routine: 2 years’ successful experience

2020 ◽  
Vol 69 (12) ◽  
pp. 1398-1404
Author(s):  
Miriam Cordovana ◽  
Anna Zignoli ◽  
Simone Ambretti
Keyword(s):  
Sample Preparation ◽  
Causative Agent ◽  
Type Species ◽  
Susceptibility Testing ◽  
Preparation Method ◽  
Bloodstream Infections ◽  
Routine Practice ◽  
Sample Preparation Method ◽  
Content Type ◽  
Link Type

Introduction. Rapid identification of the causative agent of sepsis is crucial for patient outcomes. Aim. The Sepsityper sample preparation method enables direct microbial identification of positive blood culture samples via matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS). Hypothesis/Gap statement. The implementation of the Sepsityper method in the routine practice could represent a fundamental tool to achieve a prompt identification of the causative agent of bloodstream infections, and therefore accelerate the adoption of the proper antibiotic treatment. Methodology. In this study, the novel rapid workflow of the MALDI Biotypr Sepsityper kit (Bruker Daltonik GmbH, Germany) was evaluated using routine samples from a 2-year period (n=6918), and dedicated optimized protocols for the microbial groups that were more difficult to identify were developed. Moreover, the use of the residual bacterial pellet to perform susceptibility testing using different methods (commercial broth microdilution, disc diffusion, gradient diffusion) was investigated. Results. The rapid Sepsityper protocol allowed the identification of 5470/6338 (86.3 %) monomicrobial samples at species level, with very good performance for all of the clinically most significant pathogens (2510/2592 enterobacteria, 631/669 Staphylococcus aureus and 223/246 enterococci were identified). Streptococcus pneumoniae , Bacteroides fragilis and yeasts were the most troublesome to identify, but the application of specific optimized protocols significantly improved their rate of identification (from 14.7–71.5 %, 47.8–89.7 % and 37.1–89.5 %, respectively). Specificity was 100 % (no identification was made for the false-positive samples). Further, the residual pellet proved to be suitable to investigate susceptibility to antimicrobials, enabling us to simplify the workflow and shorten the time to report. Conclusion. The Rapid Sepsityper workflow proved to be a reliable sample preparation method for identification and susceptibility testing directly from positive blood cultures, providing novel approaches for accelerated diagnostics of bloodstream infections.

Development of a sample preparation method for acrylamide determination in cocoa via silylation

2016 ◽  
Vol 8 (29) ◽  
pp. 5874-5880 ◽  
Author(s):  
Magdalena Surma ◽  
Anna Sadowska-Rociek ◽  
Ewa Cieślik
Keyword(s):  
Sample Preparation ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
Cocoa Powder ◽  
Simple Sample Preparation

This paper reports the development of a rapid and simple sample preparation method for acrylamide (AA) determination in cocoa powder through conversion to N,O-bis(trimethylsilyl)acrylamide.

A green and simple sample preparation method to determine pesticides in rice using a combination of SPME and rotating disk sorption devices

2019 ◽  
Vol 1069 ◽  
pp. 57-65 ◽  
Author(s):  
Eduarda Omena ◽  
Anderson Luiz Oenning ◽  
Josias Merib ◽  
Pablo Richter ◽  
Milton Rosero-Moreano ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Preparation Method ◽  
Rotating Disk ◽  
Sample Preparation Method ◽  
Simple Sample Preparation

scholarly journals Validation of a simple sample preparation method for multielement analysis of bovine serum

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0211859 ◽  
Author(s):  
Diego Luna ◽  
Marta Miranda ◽  
Antonio Humberto Hamad Minervino ◽  
Verónica Piñeiro ◽  
Carlos Herrero-Latorre ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Bovine Serum ◽  
Preparation Method ◽  
Multielement Analysis ◽  
Sample Preparation Method ◽  
Simple Sample Preparation

To Eliminate Curtain Effect of FIB TEM Samples by a Combination of Sample Dicing and Backside Milling

2014 ◽  
Author(s):  
Jian-Shing Luo ◽  
Hsiu Ting Lee
Keyword(s):  
Sample Preparation ◽  
Focused Ion Beam ◽  
Preparation Method ◽  
Low Cost ◽  
Ion Beam ◽  
Sample Preparation Method ◽  
Site Specific ◽  
Dual Beam ◽  
Transmission Electron

Abstract Several methods are used to invert samples 180 deg in a dual beam focused ion beam (FIB) system for backside milling by a specific in-situ lift out system or stages. However, most of those methods occupied too much time on FIB systems or requires a specific in-situ lift out system. This paper provides a novel transmission electron microscopy (TEM) sample preparation method to eliminate the curtain effect completely by a combination of backside milling and sample dicing with low cost and less FIB time. The procedures of the TEM pre-thinned sample preparation method using a combination of sample dicing and backside milling are described step by step. From the analysis results, the method has applied successfully to eliminate the curtain effect of dual beam FIB TEM samples for both random and site specific addresses.

Cross-Section Sample Preparation Method for Imaging Dopant Related Anomalies Using Scanning Probe Microscopy Techniques

2014 ◽  
Author(s):  
Swaminathan Subramanian ◽  
Khiem Ly ◽  
Tony Chrastecky
Keyword(s):  
Sample Preparation ◽  
Failure Analysis ◽  
Cross Section ◽  
Scanning Probe Microscopy ◽  
Preparation Method ◽  
Fault Isolation ◽  
Scanning Probe ◽  
Sample Preparation Method ◽  
Probe Microscopy ◽  
Section Sample

Abstract Visualization of dopant related anomalies in integrated circuits is extremely challenging. Cleaving of the die may not be possible in practical failure analysis situations that require extensive electrical fault isolation, where the failing die can be submitted of scanning probe microscopy analysis in various states such as partially depackaged die, backside thinned die, and so on. In advanced technologies, the circuit orientation in the wafer may not align with preferred crystallographic direction for cleaving the silicon or other substrates. In order to overcome these issues, a focused ion beam lift-out based approach for site-specific cross-section sample preparation is developed in this work. A directional mechanical polishing procedure to produce smooth damage-free surface for junction profiling is also implemented. Two failure analysis applications of the sample preparation method to visualize junction anomalies using scanning microwave microscopy are also discussed.

scholarly journals Application of the Dehydration Homogeneous Liquid–Liquid Extraction (DHLLE) Sample Preparation Method for Fingerprinting of Honey Volatiles

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2277
Author(s):  
Piotr M. Kuś ◽  
Igor Jerković
Keyword(s):  
Carboxylic Acid ◽  
Sample Preparation ◽  
Preparation Method ◽  
Liquid Extraction ◽  
Sample Preparation Method ◽  
Phenyllactic Acid ◽  
Homogeneous Liquid ◽  
Liquid Liquid Extraction ◽  
Wide Range ◽  
Chemical Groups

Recently, we proposed a new sample preparation method involving reduced solvent and sample usage, based on dehydration homogeneous liquid–liquid extraction (DHLLE) for the screening of volatiles and semi-volatiles from honey. In the present research, the method was applied to a wide range of honeys (21 different representative unifloral samples) to determine its suitability for detecting characteristic honey compounds from different chemical classes. GC-FID/MS disclosed 130 compounds from different structural and chemical groups. The DHLLE method allowed the extraction and identification of a wide range of previously reported specific and nonspecific marker compounds belonging to different chemical groups (including monoterpenes, norisoprenoids, benzene derivatives, or nitrogen compounds). For example, DHLLE allowed the detection of cornflower honey chemical markers: 3-oxo-retro-α-ionols, 3,4-dihydro-3-oxoedulan, phenyllactic acid; coffee honey markers: theobromine and caffeine; linden honey markers: 4-isopropenylcyclohexa-1,3-diene-1-carboxylic acid and 4-(2-hydroxy-2-propanyl)cyclohexa-1,3-diene-1-carboxylic acid, as well as furan derivatives from buckwheat honey. The obtained results were comparable with the previously reported data on markers of various honey varieties. Considering the application of much lower volumes of very common reagents, DHLLE may provide economical and ecological advantages as an alternative sample preparation method for routine purposes.

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