Four Gram-negative, rod-shaped bacterial strains, TF-26T, TF-28, TF-42 and TF-53, were isolated from a tidal flat in the Yellow Sea, Korea, and their taxonomic positions were determined by a polyphasic characterization. The strains grew optimally in the presence of 2–3 % (w/v) NaCl and at 30–37 °C. The predominant menaquinone detected in the four strains was MK-6. These strains contained large amounts of fatty acids C16 : 1
ω7c and/or iso-C15 : 0 2-OH, iso-C15 : 0, iso-C16 : 0 3-OH, C15 : 0 and iso-C17 : 0 3-OH. The DNA G+C contents of the four strains were 32·3–32·8 mol%. Strains TF-26T, TF-28, TF-42 and TF-53 showed 16S rRNA gene sequence similarity levels of 99·8–100 % and DNA–DNA relatedness levels of 82–87 %. The four strains exhibited 16S rRNA gene sequence similarity levels of 95·0–98·0 % to the type strains of the five current Tenacibaculum species, and DNA–DNA relatedness levels between the four strains and two phylogenetic relatives, Tenacibaculum mesophilum DSM 13764T and Tenacibaculum skagerrakense DSM 14836T, were less than 21 %. On the basis of phenotypic, phylogenetic and genetic data, strains TF-26T, TF-28, TF-42 and TF-53 were classified in the genus Tenacibaculum as members of a novel species, for which the name Tenacibaculum lutimaris sp. nov. (type strain, TF-26T=KCTC 12302T=DSM 16505T) is proposed.
Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea
