scholarly journals Isolation and Identification of a Recombinant Porcine Epidemic Diarrhea Virus With a Novel Insertion in S1 Domain

2021 ◽  
Vol 12 ◽  
Author(s):  
Dongliang Li ◽  
Yongtao Li ◽  
Yunchao Liu ◽  
Yumei Chen ◽  
Wenqiang Jiao ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Driving Forces ◽  
Viral Evolution ◽  
Animal Experiments ◽  
Central China ◽  
N Gene ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV) is the major pathogen that causes diarrhea and high mortality in newborn piglets with devastating impact to the pig industry. Recombination and mutation are the main driving forces of viral evolution and genetic diversity of PEDV. In 2016, an outbreak of diarrhea in piglets occurred in an intensive pig farm in Central China. A novel PEDV isolate (called HNAY) was successfully isolated from clinical samples. Sequence analysis and alignment showed that HNAY possessed 21-nucleotide (nt) insertion in its S1 gene, which has never been reported in other PEDV isolates. Moreover, the sequence of the insertion was identical with the sequence fragment in PEDV N gene. Notably, the HNAY strain exhibited two unique mutations (T500A and L521Y) in the neutralizing epitopes of the S1 protein that were different from those of other PEDV variant strains and CV777-based vaccine strains. Additionally, PEDV HNAY might be derived from a natural recombination between two Chinese variant PEDV strains. Animal experiments demonstrated that HNAY displayed higher pathogenicity compared with two other clinical isolates. This study lays the foundation for better understanding of the genetic evolution and molecular pathogenesis of PEDV.

scholarly journals Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods

1998 ◽  
Vol 5 (3) ◽  
pp. 412-414 ◽  
Author(s):  
Franco Guscetti ◽  
Curzio Bernasconi ◽  
Kurt Tobler ◽  
Kristien Van Reeth ◽  
Andreas Pospischil ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Immunofluorescence ◽  
Good Reliability ◽  
Diarrhea Virus ◽  
Pcr Method ◽  
Porcine Epidemic Diarrhea ◽  
Cryostat Sections ◽  
Formalin Fixed

ABSTRACT An immunohistochemistry method using formalin-fixed tissues, a direct immunofluorescence method using cryostat sections, an enzyme-linked immunosorbent assay (ELISA), and a PCR method were compared for diagnosis in a litter of weaned pigs that had been experimentally inoculated with wild-type porcine epidemic diarrhea virus (PEDV) and killed between 6 and 60 h after onset of diarrhea. The immunohistochemistry method proved to be as reliable as direct immunofluorescence for diagnosis of PEDV in tissues collected postmortem. The good reliability of ELISA for investigating clinical samples was confirmed, whereas the PCR method used was ineffective.

scholarly journals Pathogenicity and immunogenicity of a new strain of porcine epidemic diarrhea virus containing a novel deletion in the N gene

2020 ◽  
Vol 240 ◽  
pp. 108511 ◽  
Author(s):  
Xian-Wei Wang ◽  
Mi Wang ◽  
Jing Zhan ◽  
Qian-Yu Liu ◽  
Lin-lin Fang ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
N Gene ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Molecular Characterization of Porcine Epidemic Diarrhea Virus and Its New Genetic Classification Based on the Nucleocapsid Gene

Viruses ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 790
Author(s):  
Sung-Jae Kim ◽  
Van-Giap Nguyen ◽  
Thi-My-Le Huynh ◽  
Yong-Ho Park ◽  
Bong-Kyun Park ◽  
...  
Keyword(s):  
B Cell ◽  
Porcine Epidemic Diarrhea Virus ◽  
Cell Epitope ◽  
N Gene ◽  
Swine Industry ◽  
Genetic Classification ◽  
Nucleocapsid Gene ◽  
Diarrhea Virus ◽  
B Cell Epitope ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV) causes continuous, significant damage to the swine industry worldwide. By RT-PCR-based methods, this study demonstrated the ongoing presence of PEDV in pigs of all ages in Korea at the average detection rate of 9.92%. By the application of Bayesian phylogenetic analysis, it was found that the nucleocapsid (N) gene of PEDV could evolve at similar rates to the spike (S) gene at the order of 10−4 substitutions/site/year. Based on branching patterns of PEDV strains, three main N gene-base genogroups (N1, N2, and N3) and two sub-genogroups (N3a, N3b) were proposed in this study. By analyzing the antigenic index, possible antigenic differences also emerged in both the spike and nucleocapsid proteins between the three genogroups. The antigenic indexes of genogroup N3 strains were significantly lower compared with those of genogroups N1 and N2 strains in the B-cell epitope of the nucleocapsid protein. Similarly, significantly lower antigenic indexes in some parts of the B-cell epitope sequences of the spike protein (COE, S1D, and 2C10) were also identified. PEDV mutants derived from genetic mutations of the S and N genes may cause severe damage to swine farms by evading established host immunities.

scholarly journals Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from 2010 to 2011

Virus Genes ◽  
2012 ◽  
Vol 46 (2) ◽  
pp. 337-344 ◽  
Author(s):  
Xia Yang ◽  
Jin-yao Huo ◽  
Lu Chen ◽  
Feng-mei Zheng ◽  
Hong-tao Chang ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Porcine Epidemic Diarrhea Virus ◽  
Central China ◽  
Variation Analysis ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Genetic Characterization of Porcine Epidemic Diarrhea Virus in China Between 2014 and 2018: Emergence of the G1c Subtype

2020 ◽  
Vol 40 (04) ◽  
pp. 474-478
Author(s):  
Wenqiang Jiao
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Phylogenetic Trees ◽  
Genetic Characterization ◽  
Diagnostic Methods ◽  
Clinical Samples ◽  
Swine Industry ◽  
Diarrhea Virus ◽  
Positive Rate ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea virus (PEDV) has caused substantial economical loss to the Chinese swine industry. To illustrate the genetic characterization of PEDV circulating in China, 205 clinical samples between 2014 and 2018 were collected from 7 provinces in China. 93.17% (191 of 205) of the intestinal and fecal samples were positive for PEDV. 25 S1 amino acid (aa) together with 27 ORF3 genes from 8 provinces were sequenced and analyzed. The phylogenetic trees based on the S1 and ORF3 genes were constructed by the neighbor-joining method using MEGA 7 software. PEDV prevalence was 86.96% (40 of 46) of the swine farms in the 8 provinces and the PEDV positive rate was 93.17% (191 of 205) in the tested samples. Genetic analysis showed CH-JIANGXI-1-2016 CH-JIAGNXI-2-2016, CH-JIANGXI-3-2016 and CH-JIANGXI-2017 had three notable insertions or deletions occurred at aa 59-62, 160, and 139 (140) when compared to all of the strains in this study; moreover, phylogenetic analysis indicated that the four isolates formed a new branch significantly different from G1a, G1b and Indel subtype based on S1 gene: that is the G1c subtype. More research is needed to determine whether the insertions and deletions had biological influence on the virus. The results acquired in the present study showed the genetic diversity of PEDV circulating in 8 provinces, providing information for the development of new diagnostic methods and new vaccines

scholarly journals The interactome of porcine epidemic diarrhea virus nucleocapsid protein

2019 ◽  
Author(s):  
Min Tan ◽  
Guofei Ding ◽  
Xinna Cai ◽  
Shengliang Cao ◽  
Fangyuan Cong ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Cellular Proteins ◽  
Economic Losses ◽  
N Gene ◽  
Effective Vaccine ◽  
Label Free ◽  
Swine Industry ◽  
N Protein ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Abstract Background Many viral proteins specifically interact with cellular proteins to facilitate virus replication. Understanding these interactions can decipher the viral infection mechanism and provide potential targets for antiviral therapy. Porcine epidemic diarrhea virus (PEDV), the agent of PED, causes numerous economic losses for the swine industry each year. Till now, no effective vaccine or drugs are available to contain this disease. As a result, it is critical urgent to elucidate the PEDV interactome. The nucleocapsid (N) of PEDV plays an important role in viral replication. Results In this study, the N gene was cloned into pEGFP-C1 and transfected into 293T cells. The interactome of N was elucidated by label-free mass spectrometry. A total of 125 cellular proteins interacting with PEDV N protein were discovered, of which 4 cellular proteins, DHX9, NCL, KAP1, TCEA1, were confirmed by pull down, immunoprecipitation, and co-localization. Conclusions The interactome of N protein supplied a powerful tool to explore the role of N in PEDV infection and therapeutic targets.

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