scholarly journals Comparison of Cell Wall Polysaccharide Composition and Structure Between Strains of Sporothrix schenckii and Sporothrix brasiliensis

2021 ◽  
Vol 12 ◽  
Author(s):  
Héctor L. Villalobos-Duno ◽  
Laura A. Barreto ◽  
Álvaro Alvarez-Aular ◽  
Héctor M. Mora-Montes ◽  
Nancy E. Lozoya-Pérez ◽  
...  
Keyword(s):  
Cell Wall ◽  
Sporothrix Schenckii ◽  
Cell Wall Composition ◽  
Cell Wall Polysaccharide ◽  
Sporothrix Brasiliensis ◽  
Composition And Structure ◽  
Causative Agents ◽  
Polysaccharide Composition ◽  
Pathogenic Process

Sporothrix schenckii, Sporothrix brasiliensis, and Sporothrix globosa are the main causative agents of sporotrichosis, a human subcutaneous mycosis. Differences in virulence patterns are associated with each species but remain largely uncharacterized. The S. schenckii and S. brasiliensis cell wall composition and virulence are influenced by the culturing media, with little or no influence on S. globosa. By keeping constant the culturing media, we compared the cell wall composition of three S. schenckii and two S. brasiliensis strains, previously described as presenting different virulence levels on a murine model of infection. The cell wall composition of the five Sporothrix spp. strains correlated with the biochemical composition of the cell wall previously reported for the species. However, the rhamnose-to-β-glucan ratio exhibits differences among strains, with an increase in cell wall rhamnose-to-β-glucan ratio as their virulence increased. This relationship can be expressed mathematically, which could be an important tool for the determination of virulence in Sporothrix spp. Also, structural differences in rhamnomannan were found, with longer side chains present in strains with lower virulence reported for both species here studied, adding insight to the importance of this polysaccharide in the pathogenic process of these fungi.

Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission

2020 ◽  
Vol 61 (4) ◽  
pp. 814-825 ◽  
Author(s):  
Ruben Parra ◽  
Miguel A Paredes ◽  
Juana Labrador ◽  
Cláudia Nunes ◽  
Manuel A Coimbra ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Separation ◽  
Cell Walls ◽  
Cell Wall Composition ◽  
Arabinogalactan Protein ◽  
Cell Wall Polysaccharide ◽  
Ripe Fruit ◽  
Olive Fruit ◽  
Fruit Abscission ◽  
Polysaccharide Composition

Abstract Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission. However, changes in cell wall composition during natural fruit abscission are poorly understood. In olive (Olea europaea L.), some cultivars such as ‘Picual’ undergo massive natural fruit abscission after fruit ripening. This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in ‘Picual’ olive. To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ. Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides. Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones. Our results indicate that unesterified HGs are likely to contribute to cell separation in the olive fruit AZ. Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission. In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission. This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.

scholarly journals Dependence of Plant Cell Wall Composition and Structure on Cellulose Synthase-Like Knock Out Mutant

2012 ◽  
Vol 102 (3) ◽  
pp. 590a-591a
Author(s):  
Andreia M. Smith-Moritz ◽  
Jeemeng Lao ◽  
Joshua L. Heazlewood ◽  
Pamela C. Ronald ◽  
Miguel E. Vega-Sanchez
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cellulose Synthase ◽  
Cell Wall Composition ◽  
Knock Out ◽  
Composition And Structure

Ripening-related changes in raspberry cell wall composition and structure

2001 ◽  
Vol 56 (5) ◽  
pp. 423-428 ◽  
Author(s):  
Derek Stewart ◽  
Peitro P.M. Iannetta ◽  
Howard V. Davies
Keyword(s):  
Cell Wall ◽  
Cell Wall Composition ◽  
Composition And Structure

scholarly journals Relationship between Texture and Cell Wall Polysaccharides of Fruit Flesh in Various Species of Citrus

HortScience ◽  
1996 ◽  
Vol 31 (1) ◽  
pp. 114-116 ◽  
Author(s):  
Noboru Muramatsu ◽  
Toshio Takahara ◽  
Kiyohide Kojima ◽  
Tatsushi Ogata
Keyword(s):  
Cell Wall ◽  
Ethylenediaminetetraacetic Acid ◽  
Sugar Content ◽  
Correlation Coefficients ◽  
Cell Wall Polysaccharide ◽  
Cell Wall Polysaccharides ◽  
Satsuma Mandarin ◽  
Fruit Flesh ◽  
Polysaccharide Composition ◽  
The Relationship

Various species and cultivars of citrus were studied to determine the relationship between texture and cell wall polysaccharide content of fruit flesh. Among those tested cultivars, navel orange (Citrus sinensis Osbeck) and hassaku (C. hassaku Hort. ex Tanaka) were firmest, `Fukuhara orange' (C. sinensis Osbeck) was intermediate, and satsuma mandarin (C. unshiu Marc.) was softest. There was a 3-fold difference in firmness among the 12 citrus cultigens measured. Cohesiveness values ranged from 0.30 to 0.49 and were not correlated with fruit firmness. Sugar content in each cell wall fraction was highest in the water and EDTA fractions, followed by the hemicellulose fraction, and was lowest in the cellulose fraction. Correlation coefficients between firmness and sugar content ranged from 0.69 to 0.88 and were highest in the cellulose fraction. This study suggests that firmness of fruit flesh among the cultigens is influenced by cell wall polysaccharide composition. Chemical name used: ethylenediaminetetraacetic acid (EDTA).

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