Phosphate-Solubilizing Bacterium Acinetobacter pittii gp-1 Affects Rhizosphere Bacterial Community to Alleviate Soil Phosphorus Limitation for Growth of Soybean (Glycine max)

Phosphorus (P) availability is a major restriction to crop production, and phosphate-solubilizing bacteria (PSBs) in soils are responsible for P turnover. However, it remains unknown whether the application of PSB can facilitate both inorganic and organic P transformation and enhance function of plant rhizosphere bacteria. In this study, we applied Illumina MiSeq sequencing, plate-colony counting, quantitative PCR, and multiple ecological analyses. We found that the inoculation of PSB Acinetobacter pittii gp-1 significantly promoted the growth of soybean represented by better vegetation properties (e.g., plant height and root P) and increased activities of phosphatase (4.20–9.72 μg/g/h) and phytase (0.69–1.53 μmol/g/day) as well as content of indole acetic acid (5.80–40.35 μg/g/h). Additionally, the application of strain A. pittii gp-1 significantly increased abundances of both inorganic and organic P-cycling-related genes (i.e., phoD, bpp, gcd, and pstS). More importantly, the application of A. pittii gp-1 could increase the function represented by P-cycling-related enzymes (e.g., phosphotransferase) of rhizosphere bacterial community based on functional profiling. To our knowledge, this is the first report that the application of PSB A. pittii promotes inorganic and organic P utilization and increases the function of rhizosphere bacterial community. Therefore, the PSB A. pittii gp-1 could be a good candidate for the promotion of soybean growth.

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Contribution of Arbuscular Mycorrhizal Fungi, Phosphate–Solubilizing Bacteria, and Silicon to P Uptake by Plant

Frontiers in Plant Science ◽

10.3389/fpls.2021.699618 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hassan Etesami ◽

Byoung Ryong Jeong ◽

Bernard R. Glick

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Crop Production ◽

Current Knowledge ◽

Arbuscular Mycorrhizal ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

P Deficiency ◽

Phosphate Solubilizing ◽

P Fertilizers

Phosphorus (P) availability is usually low in soils around the globe. Most soils have a deficiency of available P; if they are not fertilized, they will not be able to satisfy the P requirement of plants. P fertilization is generally recommended to manage soil P deficiency; however, the low efficacy of P fertilizers in acidic and in calcareous soils restricts P availability. Moreover, the overuse of P fertilizers is a cause of significant environmental concerns. However, the use of arbuscular mycorrhizal fungi (AMF), phosphate–solubilizing bacteria (PSB), and the addition of silicon (Si) are effective and economical ways to improve the availability and efficacy of P. In this review the contributions of Si, PSB, and AMF in improving the P availability is discussed. Based on what is known about them, the combined strategy of using Si along with AMF and PSB may be highly useful in improving the P availability and as a result, its uptake by plants compared to using either of them alone. A better understanding how the two microorganism groups and Si interact is crucial to preserving soil fertility and improving the economic and environmental sustainability of crop production in P deficient soils. This review summarizes and discusses the current knowledge concerning the interactions among AMF, PSB, and Si in enhancing P availability and its uptake by plants in sustainable agriculture.

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Inorganic and organic phosphate solubilization potential of Stenotrophomonas maltophilia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/948/1/012054 ◽

2021 ◽

Vol 948 (1) ◽

pp. 012054

Author(s):

Suliasih ◽

S Widawati

Keyword(s):

Acid Phosphatase ◽

Stenotrophomonas Maltophilia ◽

Phosphate Solubilization ◽

Peat Soil ◽

Organic Phosphate ◽

Broth Culture ◽

Phosphate Solubilizing Bacteria ◽

Organic P ◽

Phosphate Solubilizing ◽

Inorganic And Organic

Abstract Most soils contain Phosphorus (P) in insoluble compounds as organic and inorganic forms which is unavailable to plants. Furthermore, phosphate solubilizing bacteria (PSB) play an important role in converting insoluble P to a bioavailable form through solubilizing and mineralizing inorganic or organic P. Therefore, this study aims to determine the ability of isolate a phosphate solubilizing bacterium (PSB), Stenotrophomonas maltophilia to solubilize inorganic and organic P. The bacterium was isolated from peat soil of West Kalimantan using Pikovskaya medium added with Ca3 (PO4)2 as the P source. The activity of organic P (acid phosphatase and phytase) was measured using p-nitrophenyl phosphate disodium (pNPP 0.115 M) and sodium phytate as the substrate of bacterial broth culture. Also, the optimization for inorganic and organic P solubilization by the bacterium was conducted. The maximum values of inorganic and organic phosphate solubilization was recorded to be 52.26 μg/mL and 44.51 U/mL (acid phosphatase), 0.13 U/mL (phytase) respectively. Optimum conditions found were temperature at 30 °C, pH 6.0 and in the presence of sucrose and beef extract, which serve as carbon and nitrogen sources, respectively. Therefore, it can probably be used as a biological fertilizer for plants.

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Management of Phosphorus in Salinity-Stressed Agriculture for Sustainable Crop Production by Salt-Tolerant Phosphate-Solubilizing Bacteria—A Review

Agronomy ◽

10.3390/agronomy11081552 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1552

Author(s):

Gobinda Dey ◽

Pritam Banerjee ◽

Raju-Kumar Sharma ◽

Jyoti-Prakash Maity ◽

Hassan Etesami ◽

...

Keyword(s):

Plant Growth ◽

Salinity Stress ◽

Crop Production ◽

Saline Soils ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Negative Effects ◽

P Fertilizer ◽

Low Efficiency ◽

Phosphate Solubilizing

Among the environmental factors, soil salinity is one of the most detrimental factors affecting plant growth and productivity. Nutritional-imbalance is also known as one of the negative effects of salinity on plant growth and productivity. Among the essential plant nutrients, phosphorus (P) is a nutrient in which the uptake, transport, and distribution in plant is adversely affected by salinity-stress. Salinity-stress-mediated low a P availability limits the crop production. Adding additional P fertilizer is generally recommended to manage P deficit in saline-soils; however, the low-efficiency of available P fertilizer use in salt-affected soils, restricts P availability, and P fertilizers are also a cause of significant environmental concerns. The application of salinity-tolerant phosphate–solubilizing-bacteria (ST-PSB) can be as a greatly effective and economical way to improve the P availability, and recover the P-deficit in saline-land. This review focuses on soil salinization and its effect on P availability, the mechanisms of P solubilization by ST-PSB, ST-PSB diversity, their role in alleviating salinity stress in plants, the current and future scenarios of their use, and the potential application of this knowledge to manage the sustainable environmental system. According to this review, adding ST-PSB to saline soils could be an alternative for alleviating the negative effects of salinity on plants and may ameliorate salinity tolerance.

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Biochemical activity and bioassay on maize seedling of selected indigenous phosphate-solubilizing bacteria isolated from the acid soil ecosystem

Open Agriculture ◽

10.1515/opag-2020-0036 ◽

2020 ◽

Vol 5 (1) ◽

pp. 300-304

Author(s):

Betty Natalie Fitriatin ◽

Dita Fauziah ◽

Fabira Nur Fitriani ◽

Dewi Nurma Ningtyas ◽

Pujawati Suryatmana ◽

...

Keyword(s):

Acid Soil ◽

Block Design ◽

Maize Seedling ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Soil Ecosystem ◽

Maize Productivity ◽

Randomized Block Design ◽

Phosphate Solubilizing ◽

Microbial Fertilizers

AbstractPhosphorus availability is the major constraint for plant growth in the acid soil ecosystem, due to high fixation by Al and Fe. Microbial fertilizers such as phosphate-solubilizing bacteria (PSB) can increase P availability in soils for root uptake. The objective of the research was to verify the ability of four isolates of PSB isolated from acid soil to solubilize unavailable inorganic phosphate, produce phosphatase, malic acid and indole acetic acid (IAA), as well as increase plant height of maize seedling. The bioassay by growing maize seedling in liquid nutrients has been performed to study the response of seedling to PSB inoculation. The experimental design of bioassay was a randomized block design with five replications. The results showed that the isolates RR 1 and SPR 4 had a relatively high solubilizing index. Moreover, all the PSB isolates had the ability to produce phosphatase and IAA and dissolve P. The performance of PSB-inoculated seedling was better visually and the root length was increased by 66.7–74.5% compared to the control. This result concludes that the species of four isolates needs to be identified by a biomolecular method and formulated as biofertilizers for increasing the maize productivity in the acid soil ecosystem.

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Responses of Phosphate-Solubilizing Microorganisms Mediated Phosphorus Cycling to Drought-Flood Abrupt Alternation in Summer Maize Field Soil

Frontiers in Microbiology ◽

10.3389/fmicb.2021.768921 ◽

2022 ◽

Vol 12 ◽

Author(s):

Wuxia Bi ◽

Baisha Weng ◽

Denghua Yan ◽

Hao Wang ◽

Mengke Wang ◽

...

Keyword(s):

Driving Forces ◽

Soil Microbial Communities ◽

Mature Stage ◽

Phosphate Solubilizing Bacteria ◽

Field Soil ◽

Summer Maize ◽

Maize Field ◽

P Cycling ◽

Plant P Uptake ◽

Phosphate Solubilizing

Soil microbial communities are essential to phosphorus (P) cycling, especially in the process of insoluble phosphorus solubilization for plant P uptake. Phosphate-solubilizing microorganisms (PSM) are the dominant driving forces. The PSM mediated soil P cycling is easily affected by water condition changes due to extreme hydrological events. Previous studies basically focused on the effects of droughts, floods, or drying-rewetting on P cycling, while few focused on drought-flood abrupt alternation (DFAA), especially through microbial activities. This study explored the DFAA effects on P cycling mediated by PSM and P metabolism-related genes in summer maize field soil. Field control experiments were conducted to simulate two levels of DFAA (light drought-moderate flood, moderate drought-moderate flood) during two summer maize growing periods (seeding-jointing stage, tasseling-grain filling stage). Results showed that the relative abundance of phosphate-solubilizing bacteria (PSB) and phosphate-solubilizing fungi (PSF) increased after DFAA compared to the control system (CS), and PSF has lower resistance but higher resilience to DFAA than PSB. Significant differences can be found on the genera Pseudomonas, Arthrobacter, and Penicillium, and the P metabolism-related gene K21195 under DFAA. The DFAA also led to unstable and dispersed structure of the farmland ecosystem network related to P cycling, with persistent influences until the mature stage of summer maize. This study provides references for understanding the micro process on P cycling under DFAA in topsoil, which could further guide the DFAA regulations.

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Effect of Nanoparticle Phosphate Rock and Phosphate Solubilizing Fungi on Soil P-Potential, P-Retention, Organic Carbon and Base Saturation on Cilembu’s Inceptisols

Materials Science Forum ◽

10.4028/www.scientific.net/msf.1044.143 ◽

2021 ◽

Vol 1044 ◽

pp. 143-150

Author(s):

Muhammad Amir Solihin ◽

Pujawati Suryatmana ◽

Fajri Syahid Nurhakim ◽

Rina Devnita ◽

Mahfud Arifin

Keyword(s):

Soil Properties ◽

Crop Production ◽

Phosphate Rock ◽

Base Saturation ◽

Organic P ◽

Soil P ◽

Randomized Design ◽

Completely Randomized Design ◽

Phosphate Solubilizing ◽

P Retention

Intensive agricultural cultivation on Cilembu’s Inceptisols has become several soil properties problem for crop production. Nanoparticle phosphate rock and phosphate solubilizing fungi have ability to increase some soil properties content. The research aimed to observe the effect of nanoparticle phosphate rock and phosphate solubilizing fungi on soil P-potential, P-retention, C-organic and base saturation on Cilembu’s Inceptisols. The experiment arranged in Completely Randomized Design. The nanoparticle phosphate rock consisted of 4 levels. The phosphate solubilizing fungi consisted of 2 levels, and 2 replication. Soil P-Potential, P-Retention, C-organic, and Base Saturation were observed. The factors was observed after one month incubation on soil. Results showed that there were an interaction between nanoparticle phosphate rock and phosphate solubilizing fungi on the C-organic after one month incubation. Nanoparticle phosphate rock had affected on C-organic, P-potential and base saturation significantly, but had not affected on P-retention. Utilization of phosphate solubilizing fungi as soil ameliorant had affected significantly on P-potential and C-organic, but had not affected on P-retention and base saturation. Nanoparticle phosphate rock with a dose of 6% had the best effect on increase P-potential and base saturation

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Integrated Effects of Co-Inoculation with Phosphate-Solubilizing Bacteria and N2-Fixing Bacteria on Microbial Population and Soil Amendment Under C Deficiency

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16132442 ◽

2019 ◽

Vol 16 (13) ◽

pp. 2442 ◽

Cited By ~ 1

Author(s):

Zhikang Wang ◽

Ziyun Chen ◽

Xiangxiang Fu

Keyword(s):

Plant Growth ◽

Soil Properties ◽

Soil Amendment ◽

Growth Promotion ◽

Synergistic Effects ◽

Available P ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Unsterilized Soil ◽

Phosphate Solubilizing

The inoculation of beneficial microorganisms to improve plant growth and soil properties is a promising strategy in the soil amendment. However, the effects of co-inoculation with phosphate-solubilizing bacteria (PSB) and N2-fixing bacteria (NFB) on the soil properties of typical C-deficient soil remain unclear. Based on a controlled experiment and a pot experiment, we examined the effects of PSB (M: Bacillus megaterium and F: Pseudomonas fluorescens), NFB (C: Azotobacter chroococcum and B: Azospirillum brasilence), and combined PSB and NFB treatments on C, N, P availability, and enzyme activities in sterilized soil, as well as the growth of Cyclocarya Paliurus seedlings grow in unsterilized soil. During a 60-day culture, prominent increases in soil inorganic N and available P contents were detected after bacteria additions. Three patterns were observed for different additions according to the dynamic bacterial growth. Synergistic effects between NFB and PSB were obvious, co-inoculations with NFB enhanced the accumulation of available P. However, decreases in soil available P and N were observed on the 60th day, which was induced by the decreases in bacterial quantities under C deficiency. Besides, co-inoculations with PSB and NFB resulted in greater performance in plant growth promotion. Aimed at amending soil with a C supply shortage, combined PSB and NFB treatments are more appropriate for practical fertilization at intervals of 30–45 days. The results demonstrate that co-inoculations could have synergistic interactions during culture and application, which may help with understanding the possible mechanism of soil amendment driven by microorganisms under C deficiency, thereby providing an alternative option for amending such soil.

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Effect of organic acids production and bacterial community on the possible mechanism of phosphorus solubilization during composting with enriched phosphate-solubilizing bacteria inoculation

Bioresource Technology ◽

10.1016/j.biortech.2017.09.092 ◽

2018 ◽

Vol 247 ◽

pp. 190-199 ◽

Cited By ~ 72

Author(s):

Yuquan Wei ◽

Yue Zhao ◽

Mingzi Shi ◽

Zhenyu Cao ◽

Qian Lu ◽

...

Keyword(s):

Bacterial Community ◽

Organic Acids ◽

Phosphate Solubilizing Bacteria ◽

Phosphorus Solubilization ◽

Phosphate Solubilizing

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Phosphate Solubilization Potentials of Acinetobacter Strains and their Relations with Soil Properties

Eurasian Chemico-Technological Journal ◽

10.18321/ectj50 ◽

2010 ◽

Vol 12 (3,4) ◽

pp. 231 ◽

Cited By ~ 1

Author(s):

M. Ogut ◽

F. Er ◽

N. Kandemir

Keyword(s):

Phosphate Solubilization ◽

Soil Phosphorus ◽

Soil Samples ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Batch Cultures ◽

Phosphate Solubilizers ◽

Gene Sequence Analysis ◽

Initial Ph ◽

Phosphate Solubilizing

Phosphate solubilizing bacteria can be used as soil or seed inoculum to increase soil phosphorus (P) availability for agricultural purposes. There is also a possibility of using these microorganisms to biotechnologically dissolve phosphate ores for the production of phosphorus fertilizers. Twenty-one soil samples were collected along a highway in Turkey to isolate phosphate solubilizing bacteria. A total of 20 phosphate solubilizers were isolated from the rhizosphere of wheat and maize grown in the pots, which contained the collected soil samples. The isolates were distributed among the genera, Acinetobacter (7), Pseudomonas (7), Enterobacter (2), Enterococcus (1), Escherichia (1), Photorhabdus (1), and Bacillus (1) as determined by the 16S rDNA gene sequence analysis. Since the Acinetobacter species were most effective in Pikovskaya’s agar, which contained tricalcium phosphate for the sole P-source, they were further experimented for the phosphate solubilization in batch cultures. The mean phosphorus dissolved in 5 day incubation ranged between 167 and 1022 ppm P. The initial pH of 7.8 dropped below 4.7 in six isolates with a gluconic acid production in the concentrations ranging between 27.5 and 37.5 mM. Acinetobacter isolates have some potential as an inoculum both for soil and biotechnological P-solubilization.

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