scholarly journals HOTAIR Modulated Pathways in Early-Stage Breast Cancer Progression

2021 ◽  
Vol 11 ◽  
Author(s):  
Martin C. Abba ◽  
María Laura Fabre ◽  
Jaeho Lee ◽  
Pradeep Tatineni ◽  
Hyunsuk Kil ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Signaling Pathways ◽  
Cancer Progression ◽  
Ductal Carcinoma ◽  
Early Stage ◽  
Breast Cancer Progression ◽  
Early Stage Breast Cancer ◽  
Cell Growth And Migration ◽  
Mesenchymal Transition ◽  
Over Expression

The long-non-coding HOX transcript antisense intergenic RNA (HOTAIR) was identified as significantly upregulated in breast ductal carcinoma in situ (DCIS). The aim of this study was to characterize the phenotypic effects and signaling pathways modulated by HOTAIR in early-stage breast cancer progression. We determined that HOTAIR induces premalignant phenotypic changes by increasing cell proliferation, migration, invasion and in vivo growth in normal and DCIS breast cell lines. Transcriptomic studies (RNA-seq) identified the main signaling pathways modulated by HOTAIR which include bioprocesses related to epithelial to mesenchymal transition, cell migration, extracellular matrix remodeling and activation of several signaling pathways (HIF1A, AP1 and FGFR). Similar pathways were identified as activated in primary invasive breast carcinomas with HOTAIR over-expression. We conclude that HOTAIR over-expression behaves as a positive regulator of cell growth and migration both in normal and DCIS breast cells involved with early-stage breast cancer progression.

scholarly journals LINC00885 a Novel Oncogenic Long Non-Coding RNA Associated with Early Stage Breast Cancer Progression

2020 ◽  
Vol 21 (19) ◽  
pp. 7407
Author(s):  
Martin C. Abba ◽  
Romina Canzoneri ◽  
Agustina Gurruchaga ◽  
Jaeho Lee ◽  
Pradeep Tatineni ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Signaling Pathways ◽  
Cancer Progression ◽  
Ductal Carcinoma ◽  
Early Stage ◽  
Breast Cancer Progression ◽  
Early Stage Breast Cancer ◽  
Protein Coding ◽  
Breast Cells ◽  
Invasive Breast Carcinomas

Long intergenic non-protein coding RNA 885 (LINC00885) was identified as significantly upregulated in breast ductal carcinoma in situ (DCIS). The aim of this study was to characterize the phenotypic effects and signaling pathways modulated by LINC00885 in non-invasive and invasive breast cancer models. We determined that LINC00885 induces premalignant phenotypic changes by increasing cell proliferation, motility, migration and altering 3D growth in normal and DCIS breast cell lines. Transcriptomic studies (RNA-seq) identified the main signaling pathways modulated by LINC00885, which include bioprocesses related to TP53 signaling pathway and proliferative signatures such as activation of EREG, EGFR and FOXM1 pathways. LINC00885 silencing in breast cancer lines overexpressing this lncRNA leads to downregulation of proliferation related transcripts such as EREG, CMYC, CCND1 and to significant decrease in cell migration and motility. TCGA-BRCA data analyses show an association between high LINC00885 expression and worse overall survival in patients with primary invasive breast carcinomas (p = 0.024), suggesting that the pro-tumorigenic effects of LINC00885 overexpression persist post-invasion. We conclude that LINC00885 behaves as a positive regulator of cell growth both in normal and DCIS breast cells possibly operating as a ceRNA and representing a novel oncogenic lncRNA associated with early stage breast cancer progression.

Abstract 5031: Regulatory T cell ablation accelerates early stage breast cancer progression

2019 ◽  
Author(s):  
Wei Du ◽  
Leandro Martinez ◽  
Valentina Robila ◽  
Nicholas Clark ◽  
Michael Idowu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
T Cell ◽  
Cancer Progression ◽  
Regulatory T Cell ◽  
Early Stage ◽  
Breast Cancer Progression ◽  
Early Stage Breast Cancer ◽  
Cell Ablation

Abstract 5031: Regulatory T cell ablation accelerates early stage breast cancer progression

2019 ◽  
Author(s):  
Wei Du ◽  
Leandro Martinez ◽  
Valentina Robila ◽  
Nicholas Clark ◽  
Michael Idowu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
T Cell ◽  
Cancer Progression ◽  
Regulatory T Cell ◽  
Early Stage ◽  
Breast Cancer Progression ◽  
Early Stage Breast Cancer ◽  
Cell Ablation

scholarly journals ID2 and GJB2 promote early-stage breast cancer progression by regulating cancer stemness

2019 ◽  
Vol 175 (1) ◽  
pp. 77-90 ◽  
Author(s):  
Yin Liu ◽  
Puspa R. Pandey ◽  
Sambad Sharma ◽  
Fei Xing ◽  
Kerui Wu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Progression ◽  
Early Stage ◽  
Breast Cancer Progression ◽  
Early Stage Breast Cancer ◽  
Cancer Stemness

scholarly journals LINC00665 promotes breast cancer progression through regulation of the miR-379-5p/LIN28B axis

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Wei Ji ◽  
Yu-Ling Diao ◽  
Yi-Ran Qiu ◽  
Jie Ge ◽  
Xu-Chen Cao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Progression ◽  
Epithelial Mesenchymal Transition ◽  
Normal Breast ◽  
Breast Cancer Progression ◽  
Migration And Invasion ◽  
Breast Tumorigenesis ◽  
Mesenchymal Transition ◽  
Tumorigenesis And Progression ◽  
Mirna Sponges

AbstractBreast cancer is the most common malignant tumor among women worldwide. Although increasing evidence indicates that long noncoding RNAs (lncRNAs) play critical roles during breast tumorigenesis and progression, the involvement of most lncRNAs in breast cancer remains largely unknown. In the current study, we demonstrated that LINC00665 promotes breast cancer cell proliferation, migration, and invasion. Accumulating evidence indicates that many lncRNAs can function as endogenous miRNA sponges by competitively binding common miRNAs. In this study, we demonstrated that LINC00665 functions as a sponge for miR-379-5p, reducing the ability of miR-379-5p to repress LIN28B. LINC00665 promoted breast cancer progression and induced an epithelial–mesenchymal transition-like phenotype via the upregulation of LIN28B expression. Clinically, LINC00665 expression was increased but miR-379-5p expression was decreased in breast cancer tissues compared with that in normal breast tissues in the TCGA database. Furthermore, the expression of LINC00665 was negatively related with miR-379-5p expression. Collectively, our results reveal the LINC00665–miR-379-5p–LIN28B axis and shed light on breast cancer therapy.

scholarly journals Expression of epithelial-mesenchymal transition-related markers and phenotypes during breast cancer progression

2020 ◽  
Vol 181 (2) ◽  
pp. 369-381 ◽  
Author(s):  
Charlotte Levin Tykjær Jørgensen ◽  
Carina Forsare ◽  
Pär-Ola Bendahl ◽  
Anna-Karin Falck ◽  
Mårten Fernö ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Progression ◽  
Epithelial Mesenchymal Transition ◽  
Breast Cancer Progression ◽  
Mesenchymal Transition

scholarly journals MicroRNA-1291 Is Associated With Locoregional Metastases in Patients With Early-Stage Breast Cancer

2020 ◽  
Vol 11 ◽  
Author(s):  
Daniel Escuin ◽  
Laura López-Vilaró ◽  
Olga Bell ◽  
Josefina Mora ◽  
Antonio Moral ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Signaling Pathways ◽  
Axillary Lymph Node ◽  
Target Genes ◽  
Early Stage ◽  
Early Stage Breast Cancer ◽  
Axillary Lymph ◽  
Illumina Platform ◽  
Down Regulation

Evidence that microRNAs (miRNAs) regulate the various steps of metastasis is increasing. Several studies have looked at the miRNA expression profile in primary breast tumors but few have compared primary tumor and sentinel lymph node (SLN) metastasis. We correlated the expression of miRNAs with the SLN status and the outcome of axillary lymph node dissection (ALND) in 60 patients with early breast cancer. We profiled the expression of miRNAs in paired breast tumor samples and SLNs using the NextSeq500 Illumina platform and key findings were validated by qPCR. MultiMiR Bioconductor and Reactome pathways analysis were performed to identify target genes and signaling pathways affected by altered expressed miRNAs. Our results show that nine miRNAs were differentially expressed in tumor tissues (q ≤ 0.05). In tumor samples, a 13.5-fold up-regulation of miR-7641-2 (q < 0.001) and a 2.9-fold down-regulation of miR-1291 (q < 0.001) were associated with tumors with positive SLNs. However, only down-regulation of miR-1291 (q = 0.048) remained significant in paired SLNs samples. Interestingly, a 10.5 up-regulation of miR-1291 in SLNs samples was associated with additional axillary lymph node involvement (q < 0.001). The enrichment analyses showed that canonical and non-canonical WNT pathways and negative regulation of various receptor tyrosine kinases signaling pathways were targets of miR-1291 and supports the role of miR-1291 as a tumor suppressor gene (TSG). Further studies are warranted to investigate the use of miR-1291 as a surrogate biomarker of SLN node metastasis in patients with early-stage breast cancer.

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