scholarly journals Five Constituents Contributed to the Psoraleae Fructus-Induced Hepatotoxicity via Mitochondrial Dysfunction and Apoptosis

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhaojuan Guo ◽  
Pin Li ◽  
Chunguo Wang ◽  
Qianjun Kang ◽  
Can Tu ◽  
...  
Keyword(s):  
Animal Experiments ◽  
Extraction Process ◽  
Cell Counting ◽  
Severe Injuries ◽  
Ethanol Extraction ◽  
Intracellular Lipid Accumulation ◽  
Q Exactive ◽  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer ◽  
Hepg2 Cell Lines

Backgrounds: Psoraleae Fructus (PF)-induced hepatotoxicity has been reported in clinical and animal experiments. However, the hepatotoxic constituents and mechanisms underlying PF-induced toxicity have remained unclear. Therefore, this study explored the potentially toxic PF components and revealed their relative mechanisms.Methods: The hepatotoxicity of PF water (PFW) and ethanol (PFE) extracts was compared using Kunming mice. The different compositions between PFW and PFE, which were considered toxic compositions, were identified using the UHPLC-Q-Exactive MS method. Then, L02 and HepG2 cell lines were used to evaluate the toxicity of these compositions. Cell viability and apoptosis were determined through the Cell Counting Kit-8 (CCK-8) assay and flow cytometry, respectively. An automatic biochemical analyzer detected the aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). Lastly, we used high-content screening (HCS) to determine the levels of reactive oxygen species (ROS), lipid, and mitochondrial membrane potential (MMP).Results: The ethanol extraction process aggravated the hepatotoxicity of PF, causing more severe injuries. The content of psoralen, isopsoralen, bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol was higher in the PFE than PFW. Bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol induced cell apoptosis and the AST, ALT, and ALP leakages. Furthermore, these five constituents increased intracellular lipid accumulation and ROS levels but decreased the MMP level.Conclusion: The ethanol extraction process could induce severe PF hepatotoxicity. Bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol are the main hepatotoxic ingredients. This mechanism could be associated with oxidative stress and mitochondrial damage-mediated apoptosis. Taken together, this study provides a basis for the clinical application of PF that formulates and improves its herbal standards.

111 BIOCHEMICAL COMPOSITION OF FOLLICULAR FLUID IN RELATION TO THE STIMULUS TO INDUCE OVULATION IN ALPACAS (VICUGNA PACOS)

2017 ◽  
Vol 29 (1) ◽  
pp. 164 ◽  
Author(s):  
W. Huanca ◽  
A. Castro ◽  
N. Gomez ◽  
A. Cordero
Keyword(s):  
Follicular Fluid ◽  
Total Protein ◽  
Biochemical Composition ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Array Transducer ◽  
Vicugna Pacos ◽  
Continue Research ◽  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer

Alpacas, like other camelids, are induced ovulators. A study was designed to determine the effect of the ovulation-inducing stimulus on the biochemical composition of follicular fluid. Adult female alpacas (n = 18) were examined daily for 3 days by transrectal ultrasonography using a 5-MHz linear-array transducer (Aloka SSD-500, Tokyo, Japan). When the largest growing ovarian follicle was ≥7 mm, alpacas were given 1.0 mL of seminal plasma intramuscularly (SP, n = 9) or 40 µg of busereline acetate intramuscularly (GnRH, n = 9). A transvaginal transducer with an attached needle guide (Aloka UST-945BP-5) was used for collection of follicular fluid 22 h post-induction. Follicular contents were then centrifuged at 800 × g for 20 min to separate the fluid from the cells. The follicular fluid was collected and stored at –20°C until analysis with a semi-automatic biochemical analyzer (SINOWA, China). The results were glucose 49.17 and 47.95 (mg/dL; P > 0.05), total protein 1.85 and 1.15 (g/dL; P < 0.05), albumin 1.11 and 1.13 (g/dL; P > 0.05), triglycerides 3.94 and 3.16 (mg/dL; P > 0.05), cholesterol 39.01 and 42.5 (mg/dL; P > 0.05), phosphatase 32.68 and 21.36 (IU/L; P < 0.05), alanine aminotransferase 3.66 and 5.07 (IU/L; P > 0.05), and lactate dehydrogenase 42.17 and 27.27 (IU/L; P > 0.05) for SP or GnRH treatments, respectively. Results suggest the need to continue research to explain the effect of possible differences in total protein, cholesterol, and phosphatase on oocyte-expressed genes and follicular development. Research was supported by the project no. 405-PNICP-PIAP-2014-UNMSM.

AB-1 automatic biochemical analyzer

1975 ◽  
Vol 9 (6) ◽  
pp. 365-366
Author(s):  
B. M. Kotenev
Keyword(s):  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer

Determination and Ratio of Ferulic Acid and Gastrodin in Xiongma Decoction Extract by Different Extraction Process

2014 ◽  
Vol 955-959 ◽  
pp. 740-743 ◽  
Author(s):  
Lin Liu ◽  
Yue Ting Liu ◽  
Ju Huo ◽  
Zhao Ying
Keyword(s):  
Ferulic Acid ◽  
Extraction Method ◽  
Mobile Phase ◽  
Acetic Acid Solution ◽  
Extraction Process ◽  
New Drugs ◽  
Quality Inspection ◽  
Ethanol Extraction ◽  
Main Effect

Objective: The determination and ratio of ferulic acid and gastrodin in Xiongma Decoction extract by different extraction process were compared, to provide scientific research basis on creating new drugs, controlling the quality combination of modern Compound Chinese, determining the main effect and compatibility. Methods: Chuanxiong and Tianma were extracted by different extraction method such as the boiling method and ethanol extraction by different concentrations to make ​​Xiongma Decoction. The content of ferulic acid and gastrodin were determination by HPLC to make Xiongma Decoction and their ratio was calculated. Chromatographic conditions of ferulic acid: mobile phase: methanol-0.01 % acetic acid solution (30:70, V/V), the detection wavelength: 310nm; chromatographic conditions of Gastrodin: mobile phase: acetonitrile-0.05 % phosphoric acid (3:97, V/V), detection wavelength: 220nm. Results: The determination of ferulic acid was 0.976mg • ml-1, gastrodin was 1.586mg • ml-1, the ratio of ferulic acid and gastrodin was1:1.6 in boiling method; while the determination of ferulic acid was 6.08mg • ml-1, gastrodin was 3.57mg • ml-1, the ratio was 1.7:1 in ethanol extraction. Conclusion: There are different determination and ratio, when there is different extraction process in the same prescription. This may bring about changes the main effect in prescription. This suggests that the ratio of the main active ingredient should be included in the quality inspection, when preparation process of the traditional recipe was changed.

scholarly journals Extraction optimisation and lipid-lowering activity of Auricularia heimuer polysaccharides

2021 ◽  
Author(s):  
Xianghui Kong ◽  
Yinpeng Ma ◽  
Yu Pan ◽  
Wei Jiang ◽  
Dingjin Li ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Enzymatic Hydrolysis ◽  
Functional Foods ◽  
Animal Experiments ◽  
Extraction Process ◽  
Extraction Methods ◽  
Lipid Lowering ◽  
Process Optimisation ◽  
Molecular Weight Range ◽  
Lowering Activity

Assessments of molecular weight distribution and activity/efficacy of Auricularia heimuer polysaccharides (AAP) are of substantial significance for its extraction process optimisation. In the present study, single-factor orthogonal test and response surface methodology were employed to optimise extraction conditions of AAP. Furthermore, a rat hyperlipidaemia model was established to compare the lipid-lowering activity of polysaccharides obtained by three extraction methods. Conditions for enzymatic hydrolysis were optimised as pH 5.0, 1% cellulase, 2.5% substrate concentration and enzymolysis time of 1.5 h, leading to an up to 31.8% polysaccharide yield and 89.13% of polysaccharides within the molecular weight range of 5 000 Da to 10 000 Da. The results of animal experiments showed that the lipid-lowering activity of enzymolysis-extracted polysaccharides was significantly higher than that of water- and ultrasonic-extracted ones (P &lt; 0.01). So the present study revealed that enzymatic hydrolysis-extracted polysaccharides showed the strongest hypolipidaemia activity, providing a basis for the development of A. heimuer-based functional foods and drugs.

scholarly journals Validation of the biochemical method for determining the total cholesterol level in the samples of biological fluids as a base for providing the quality of dyslipidemia diagnostics

2021 ◽  
Vol 25 (1) ◽  
pp. 50-56
Author(s):  
S. V. Misiurova ◽  
N. O Svid ◽  
V. Ye Dobrova ◽  
I. A Otrishko ◽  
V. V. Propisnova
Keyword(s):  
Total Cholesterol ◽  
Biological Fluids ◽  
Total Cholesterol Level ◽  
Clinical Diagnostic Laboratory ◽  
Diagnostic Laboratory ◽  
Clinical Diagnostic ◽  
Uncertainty Of Measurements ◽  
Biochemical Analyzer ◽  
Repeatability And Reproducibility ◽  
Automatic Biochemical Analyzer

The main principles of creating a quality system in modern laboratory diagnostics are: standardization of laboratory processes by developing standard operating procedures; general quality management of laboratory research based on the development and implementation of the requirements of international standards (according to ISO 15189: 2015 "Medical laboratories. Basic requirements for quality and competence"); quality control of all stages of the laboratory process through the implementation of the validation procedure. Aim. To develop a methodology for conducting validation procedures to assess the suitability of a biochemical method for determining the level of total cholesterol in biological fluids in the Clinical Diagnostic Laboratory of the Clinical Diagnostic Center of the NUPh. Materials and methods. The object of the study was a standardized method for determining the concentration of total cholesterol. The method was validated using the “Cholesterol Reagent Set” test kit and the standard sample “Chemical control. Reagent kit. Level 1 ", manufactured by High Technology, Inc. (USA) with known concentration. The measurements were carried out on an Express Plus automatic biochemical analyzer manufactured by Bayer Corporation, Germany. When processing the research results, descriptive statistics were used and a number of statistical evaluations were carried out. Results. A protocol and a validation report were developed at the Clinical Diagnostic Laboratory of the CDC NUPh to assess the suitability of the method for determining the concentration of cholesterol in biological fluids by the photometric enzymatic method on an automatic biochemical analyzer Express Plus (using reagents and control material manufactured by High Technology, Inc., USA). The validation characteristics of the method were determined: repeatability and reproducibility, correctness and uncertainty of measurements. Evaluation of the internal laboratory repeatability and reproducibility of this technique indicates the absence of gross errors in the operation of the instrument and statistically important differences in measurements. The assessment of the correctness of the method (carried out using the control material) proved that the systematic error is not significant (according to a given acceptance criterion). The expanded uncertainty calculation showed that the obtained values ​​of the total cholesterol level can be considered accurate and reliable. Conclusions. Validation of the method for determining total cholesterol in human blood by the photometric enzymatic method has proven that this method has performance characteristics that correspond to the regulated ones, meets the established criteria, and the parameters measured with it correspond to the proper ones. Key words: validation, determination method, total cholesterol, repeatability and reproducibility, correctness and uncertainty of measurements

scholarly journals Reference intervals of the magnesium in the healthy individuals, residents of Astrakhan

2019 ◽  
Vol 10 (4) ◽  
pp. 87-91
Author(s):  
O. V. Petrova ◽  
D. M. Nikulina ◽  
M. U. Martirosov
Keyword(s):  
Serum Magnesium ◽  
Reference Intervals ◽  
Laboratory Research ◽  
Photometric Method ◽  
Healthy Individuals ◽  
Biochemical Analyzer ◽  
Reference Serum ◽  
Automatic Biochemical Analyzer ◽  
Total Magnesium

Objective: to establish reference serum magnesium intervals in healthy individuals, residing in Astrakhan.Materials and methods: the investigated group was formed from 120 men and 120 women healthy residents of Astrakhan aged from 21 to 50 years (average age 37,6 ± 0,9). Th e study of calcium was carried out on an automatic biochemical analyzer “Cobas 311 c” (Roche Diagnostic, Germany) by photometric method.Results: the reference intervals for the magnesium in healthy men and women aged from 21 to 50 years, residing in Astrakhan is 0,72 – 0,99 mmol/L.Conclusion: the reference range of total magnesium, established by us, can be used in laboratories of Astrakhan, as it was developed taking into account domestic and foreign recommendations for selection donors for research and ensuring the quality of laboratory research at all stages. 

scholarly journals The value of the acidified glycerol lysis test with a graphical determination for screening of hereditary spherocytosis

2020 ◽  
Vol 6 (6) ◽  
pp. 51-59
Author(s):  
T. T. Asatryan ◽  
L. B. Gaikovaya ◽  
V. V. Slepisheva
Keyword(s):  
Hemolytic Anemia ◽  
Reliable Method ◽  
Hereditary Spherocytosis ◽  
Search Methods ◽  
Common Cause ◽  
Clinical Diagnostic ◽  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer

Hereditary spherocytosis (HS) is the most common cause of hemolytic anemia of varying severity can lead to complications such as cholelithiasis, sepsis, etc. Difficulties in diagnosis are asymptomatic and atypical cases HS. It is important to search methods that can detect microspherocytic anemia.The article describes the technique of acidified glycerol lysis test (AGLT), the results and analytical characteristics of the method. Presents a refined acidified glycerol lysis test (AGLT) with incubated blood performed on the automatic biochemical analyzer with a graphical check of the lysis of erythrocytes is a sensitive, fast and reliable method of identification of HS and can be used in clinical diagnostic laboratories.

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