111 BIOCHEMICAL COMPOSITION OF FOLLICULAR FLUID IN RELATION TO THE STIMULUS TO INDUCE OVULATION IN ALPACAS (VICUGNA PACOS)

2017 ◽  
Vol 29 (1) ◽  
pp. 164 ◽  
Author(s):  
W. Huanca ◽  
A. Castro ◽  
N. Gomez ◽  
A. Cordero
Keyword(s):  
Follicular Fluid ◽  
Total Protein ◽  
Biochemical Composition ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Array Transducer ◽  
Vicugna Pacos ◽  
Continue Research ◽  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer

Alpacas, like other camelids, are induced ovulators. A study was designed to determine the effect of the ovulation-inducing stimulus on the biochemical composition of follicular fluid. Adult female alpacas (n = 18) were examined daily for 3 days by transrectal ultrasonography using a 5-MHz linear-array transducer (Aloka SSD-500, Tokyo, Japan). When the largest growing ovarian follicle was ≥7 mm, alpacas were given 1.0 mL of seminal plasma intramuscularly (SP, n = 9) or 40 µg of busereline acetate intramuscularly (GnRH, n = 9). A transvaginal transducer with an attached needle guide (Aloka UST-945BP-5) was used for collection of follicular fluid 22 h post-induction. Follicular contents were then centrifuged at 800 × g for 20 min to separate the fluid from the cells. The follicular fluid was collected and stored at –20°C until analysis with a semi-automatic biochemical analyzer (SINOWA, China). The results were glucose 49.17 and 47.95 (mg/dL; P > 0.05), total protein 1.85 and 1.15 (g/dL; P < 0.05), albumin 1.11 and 1.13 (g/dL; P > 0.05), triglycerides 3.94 and 3.16 (mg/dL; P > 0.05), cholesterol 39.01 and 42.5 (mg/dL; P > 0.05), phosphatase 32.68 and 21.36 (IU/L; P < 0.05), alanine aminotransferase 3.66 and 5.07 (IU/L; P > 0.05), and lactate dehydrogenase 42.17 and 27.27 (IU/L; P > 0.05) for SP or GnRH treatments, respectively. Results suggest the need to continue research to explain the effect of possible differences in total protein, cholesterol, and phosphatase on oocyte-expressed genes and follicular development. Research was supported by the project no. 405-PNICP-PIAP-2014-UNMSM.

224 OVARIAN FOLLICLE DEVELOPMENT DURING SEASONAL TRANSITION IN WAPITI

2006 ◽  
Vol 18 (2) ◽  
pp. 220
Author(s):  
R. McCorkell ◽  
M. Woodbury ◽  
G. Adams
Keyword(s):  
Breeding Season ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Maximum Diameter ◽  
Follicle Development ◽  
Estrous Cycles ◽  
Ovarian Follicle Development ◽  
Array Transducer ◽  
October 17 ◽  
Ovulatory Follicles

Wapiti are seasonally polyestrous. The transition into and out of the breeding season is marked by resumption of ovulation in autumn and cessation of ovulation in winter. Onset of ovulatory cyclicity is distinct and associated with aggressive breeding behavior of stags in rut. Cessation of ovulation at the end of the breeding season is not distinguished by behavioral patterns. The objective of the present study was to characterize follicular and luteal dynamics in wapiti during the transitional periods into and out of the breeding seasons. Transition from anestrus to estrus was monitored in 15 hinds, aged 2 to 14 years, over two successive seasons (11 in year 1, with 5 hinds from year 1 used again in year 2 along with 4 new hinds; n = 20 observations). Transition from estrus to anestrus was monitored in 11 hinds over 1 season (n = 11 observations). Hinds were maintained on a farm near Saskatoon, Saskatchewan (52°07′N, 106°38′W). The ovaries were examined daily during September through October by transrectral ultrasonography using a B-mode ultrasound machine and a 7.5 MHz linear array transducer for transition to estrus, and December through April for transition to anestrus. The first ovulation was recorded on September 15 and all hinds had ovulated for the first time by October 7. In 17 of 20 observations, the duration of the first interovulatory interval (IOI) was 9.3 ± 0.4 days (mean ± SEM). With one exception, these IOIs were characterized by one wave of follicular development. The remaining three IOIs ranged from 16 to 23 days and consisted of two or three waves of follicle development. The second ovulation occurred by October 15 in hinds with a short IOI and by October 17 in all remaining hinds. The mean dates of first and last ovulation were September 25 and February 7, respectively, an interval of 135 days. The median date of the last ovulation was February 15 and the range was from December 3 to March 22. Duration of the last IOI of the season (21.2 ± 0.6 days) was similar to the notional 21-day cycle for wapiti, but longer (P < 0.05) than the duration of the first IOI (10.9 ± 1.0 days). Maximum diameters of the first 2 ovulatory follicles were similar (11.3 ± 0.4 vs. 11.3 ± 0.2 mm), but were larger (P < 0.05) than the last 2 ovulatory follicles of the breeding season (10.3 ± 0.3 vs. 10.1 ± 0.4 mm). Maximum diameter of the corpus luteum (CL) tended (P = 0.06) to be smaller for the short IOI than for longer IOI of the first and last cycles. Diameter of the last CL of the season was not different from that of the previous CL (12.8 ± 0.6 vs. 12.5 ± 0.6 mm); however, it was detected for a longer period (22.3 ± 1.2 vs. 19.3 ± 0.7 days; P < 0.05). Estrous cycles during transition into the breeding season have been described as being irregular and those out of the breeding season as increasingly long. In the present study, the transition periods were characterized by regular events. Transition to regular estrous cycles was preceded by one short (9 days) IOI. The last IOI of the breeding season was the same as that reported during the rut. Transition to anestrus occurred most commonly in February and was marked by a failure of the dominant follicle to ovulate after luteal regression.

A Novel Method for Evaluating Compatibility of Hepatocytes with some Kinds of Biomaterials

2005 ◽  
Vol 288-289 ◽  
pp. 511-514
Author(s):  
Yingxin Xu ◽  
Wen Jie Wang ◽  
Yong Zhang ◽  
Xu Hua Song ◽  
Li Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Total Protein ◽  
Culture Medium ◽  
New Method ◽  
Morphological Observation ◽  
Hepatic Tissue ◽  
Feasible Method ◽  
Biochemical Analyzer ◽  
Novel Method ◽  
Automatic Biochemical Analyzer

To evaluate the biocompatibility of biomedical materials for hepatic tissue engineering, another new method was introduced to observe hepatocytes functions. In this experiment, hepatocytes were seeded onto four kinds of membranes of PLLA, PLGA (90:10), PLGA (75:25), and Chitosan cross-linked with collagen. The culture mediums were collected at 21 day after seeding, and then albumin (Alb), Urea (UN), glucose (Glu), total protein (TP), and triglyceride (TG) in the supernatant were detected by automatic biochemical analyzer. Results showed that hepatocytes on film of Chitosan cross-linked with collagen exhibited the highest level of TP and TG. These results were highly corresponding with the results of morphological observation. This data indicated that analyzing TP and TG in culture medium by automatic biochemical analyzer might be applied to evaluate hepatocytes biocompatibility on materials as a convenient and feasible method.

70 Effect of Ovulation Stimulus on Biochemical Composition of the Oviduct of Alpaca (Vicugna pacos)

2018 ◽  
Vol 30 (1) ◽  
pp. 173
Author(s):  
F. Y. Hilari ◽  
J. C. Villanueva ◽  
W. F. Huanca ◽  
B. Lira ◽  
W. Huanca
Keyword(s):  
Biochemical Composition ◽  
Ovulation Induction ◽  
Control Group ◽  
Gnrh Analogue ◽  
Adult Females ◽  
Vicugna Pacos ◽  
Buserelin Acetate ◽  
Biochemical Analyzer ◽  
Oviduct Fluid

Experiments were designed to determine the effect of ovulation stimulus on the biochemical composition of oviduct fluid in alpacas (Vicugna pacos). Twenty-four adult females were used to determine profiles of protein, albumin, glucose, cholesterol, and triglycerides in oviduct fluid. The animals were stimulated with 1 mL of seminal plasma (SP), 0.042 mg of a gonadotropin-releasing hormone (GnRH) analogue (buserelin acetate; Conceptal, Intervet, Holland), or PBS as control group. The oviduct was removed inmediately after slaughter on Day 2 or 4 after ovulation induction and washed with 3 mL of PBS solution. Determination of the biochemical composition was carried out with specific kits from the FAR Diagnostics Italia laboratory using a semiautomatic biochemical analyzer of the Sinowa China brand, which is based on spectrophotometric evaluation. The results are presented in Table 1. Our results suggest that the method of induction of ovulation affected the biochemical composition of oviduct fluid, including concentrations of albumin, cholesterol, total protein, triglyceride, and glucose. Table 1.Biochemical composition of oviduct fluid (mean ± SEM).

96 Follicular Fluid and Serum Biochemical Composition in Alpacas (Vicugna pacos) with Different Nutritional Planes

2018 ◽  
Vol 30 (1) ◽  
pp. 187
Author(s):  
W. Huanca ◽  
F. Hilari ◽  
M. Ticona ◽  
B. Lira ◽  
J. C. Villanueva ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Biochemical Composition ◽  
Oocyte Quality ◽  
Metabolizable Energy ◽  
High Rate ◽  
Hypodermic Needle ◽  
Limited Information ◽  
Embryo Survival ◽  
Natural Pasture ◽  
Vicugna Pacos

The importance of nutrition on composition of follicular fluid and oocyte quality is recognised in several species but limited information is available on alpacas and would explain the high rate of early embryo mortality. The aim of this study was to analyse the biochemical composition of serum and follicular fluid (FF) from alpacas (Vicugna pacos) with different nutritional planes. Twelve adult female alpacas between 6 and 8 years old were assigned randomly to either a high (n = 6) or low (n = 6) plane of nutrition. Nutritional planes were defined by the grazing condition of the natural pasture with (high) or without (low) supplementation with 200 g of concentrate/day (total digestible nutrients: 52%, protein: 16%, and metabolizable energy: 2.8 Mcal kg−1). The nutritional conditions were imposed 1 month before the start of the experiment.The concentrations of total glucose, cholesterol, triglycerides, and proteins were determined with a semi-automatic biochemical analyzer (SINOWA, China). When an ovulatory follicle ≥7 mm was detected by ultrasonography using a 5-MHz linear-array transducer (SSD-500, Aloka, Tokyo, Japan), a transvaginal transducer with an attached needle guide (UST-945BP-5, Aloka) was used to collect follicular fluid. Follicular puncture was performed using a disposable 19-gauge # 12 mm hypodermic needle connected to a 50-mL conical tube via a silicon tube. A caudal epidural anaesthesia was induced with 4 mL of 2% lidocaine. Blood samples were obtained by jugular venipuncture into serum vacutainers and kept at room temperature for 30 min. Follicular fluid and serum were centrifuged at 1500 × g for 20 min, decanted, and stored at –20°C until analysed. Results were analysed by Student’s t-test. The results are presented in Table 1. The results suggest differences in biochemical composition of glucose and cholesterol in follicular fluid, which could explain the oocyte quality and embryo survival in alpacas. Table 1.Biochemical composition of follicular fluid (FF) and serum from alpacas (Vicugna pacos) under 2 nutritional planes Research was supported by INNOVATE PERU Grant 405-PNICP-PIAP-2014.

scholarly journals Relaxin protein and gene expression in ovarian follicles of immature pigs

1998 ◽  
Vol 21 (2) ◽  
pp. 179-187 ◽  
Author(s):  
KM Ohleth ◽  
Q Zhang ◽  
CA Bagnell
Keyword(s):  
Gene Expression ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Ovarian Follicles ◽  
In Vitro Growth ◽  
Follicle Size ◽  
Theca Interna

Relaxin production by the ovarian follicle of gonadotropin-primed, prepubertal gilts is well documented. As far as we are aware, a source of relaxin in pig follicles, independent of gonadotropins, has not yet been reported. Therefore, the objective of this study was to determine whether relaxin is produced in porcine follicles in the absence of exogenous or cyclic gonadotropins. In immature pigs, immunoreactive relaxin was detected in fluids from small (1-3 mm), medium (4-5 mm) and large (>6 mm) follicles and localized to the theca interna of large follicles. Relaxin levels in follicular fluid significantly increased with follicle size (P<0.05). Relaxin mRNA was detected in whole small- and medium-sized follicles. In large follicles, the relaxin gene was expressed in thecal layers, but not granulosa cells. The abundance of relaxin transcript did not change with follicle size. In summary, relaxin protein and mRNA were detected in porcine follicles from immature animals, indicating that relaxin is produced in the porcine follicle in the absence of exogenous or cyclic gonadotropins. Relaxin's in vitro growth effects on porcine granulosa and theca cells support this follicular relaxin as a growth modulator during porcine follicular development.

Magnetic resonance image attributes of the bovine ovarian follicle antrum during development and regression

Reproduction ◽  
2000 ◽  
pp. 311-323 ◽  
Author(s):  
JL Hilton ◽  
GE Sarty ◽  
GP Adams ◽  
RA Pierson
Keyword(s):  
Magnetic Resonance ◽  
Relaxation Rate ◽  
Follicular Fluid ◽  
Magnetic Resonance Image ◽  
Follicular Development ◽  
Physiological Status ◽  
Proton Density ◽  
Dominant Follicle ◽  
Rate Heterogeneity ◽  
Pixel Value

The magnetic resonance images and maps of bovine ovaries acquired at defined phases of follicular development and regression were studied to determine whether magnetic resonance image attributes of the follicular antrum reflect the physiological status of dominant and subordinate ovarian follicles. Ovariectomies were performed at day 3 of wave one, day 6 of wave one, day 1 of wave two and at >/= day 17 after ovulation. The timings of ovariectomies were selected to acquire growing, early static, late static and regressing follicles of the first wave and preovulatory follicles of the ovulatory wave. Pre-selection and subordinate follicles were also available for analysis. Serum samples were taken on the day of ovariectomy and follicular fluid samples were taken after imaging. Numerical pixel value and pixel heterogeneity in a spot representing approximately 95% of the follicular antrum were quantified in T(1)- and T(2)-weighted images. T(1) and T(2) relaxation rates (T(1) and T(2)), proton density, apparent diffusion coefficients and their heterogeneities were determined from the computed magnetic resonance maps. The antra of early atretic dominant follicles showed higher T(2)-weighted mean pixel value (P < 0.008) and heterogeneity (P < 0. 01) and lower T(2) heterogeneity (P < 0.008) than growing follicles. Subordinate follicles in the presence of a preovulatory dominant follicle had higher T(1), T(1) heterogeneity, proton density, proton density heterogeneity, and lower mean pixel value in T(1)-weighted images than subordinate follicles of the anovulatory wave (P < 0.04). T(1) relaxation rate heterogeneity and proton density heterogeneity were positively correlated with follicular fluid oestradiol concentration (r = 0.4 and 0.3; P < 0.04). T(2) relaxation rate heterogeneity was positively correlated with follicular fluid progesterone concentration (r = 0.4; P < 0.008). Quantitative differences in magnetic resonance image attributes of the antrum observed among phases of follicular development and regression coincided with changes in the ability of the dominant follicle to produce steroid hormones and ovulate, and thus were indicative of physiological status and follicular health.

scholarly journals Genome-wide transcriptome profiling uncovers differential miRNAs and lncRNAs in ovaries of Hu sheep at different developmental stages

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Samina Shabbir ◽  
Prerona Boruah ◽  
Lingli Xie ◽  
Muhammad Fakhar-e-Alam Kulyar ◽  
Mohsin Nawaz ◽  
...  
Keyword(s):  
Target Genes ◽  
Expression Profiles ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Differentially Expressed ◽  
Ovary Development ◽  
Estrogen Metabolism ◽  
Genome Wide ◽  
Micro Rnas ◽  
Hu Sheep

AbstractOvary development is an important determinant of the procreative capacity of female animals. Here, we performed genome-wide sequencing of long non-coding RNAs (lncRNAs) and mRNAs on ovaries of 1, 3 and 8 months old Hu sheep to assess their expression profiles and roles in ovarian development. We identified 37,309 lncRNAs, 45,404 messenger RNAs (mRNAs) and 330 novel micro RNAs (miRNAs) from the transcriptomic analysis. Six thousand, seven hundred and sixteen (6716) mRNAs and 1972 lncRNAs were significantly and differentially expressed in ovaries of 1 month and 3 months old Hu sheep (H1 vs H3). These mRNAs and target genes of lncRNAs were primarily enriched in the TGF-β and PI3K-Akt signalling pathways which are closely associated with ovarian follicular development and steroid hormone biosynthesis regulation. We identified MSTRG.162061.1, MSTRG.222844.7, MSTRG.335777.1, MSTRG.334059.16, MSTRG.188947.6 and MSTRG.24344.3 as vital genes in ovary development by regulating CTNNB1, CCNA2, CDK2, CDC20, CDK1 and EGFR expressions. A total of 2903 mRNAs and 636 lncRNAs were differentially expressed in 3 and 8 months old ovaries of Hu sheep (H3 vs H8); and were predominantly enriched in PI3K-Akt, progesterone-mediated oocyte maturation, estrogen metabolism, ovulation from the ovarian follicle and oogenesis pathways. These lncRNAs were also found to regulate FGF7, PRLR, PTK2, AMH and INHBA expressions during follicular development. Our result indicates the identified genes participate in the development of the final stages of follicles and ovary development in Hu sheep.

scholarly journals Heat stress diminishes gonadotropin receptor expression and enhances susceptibility to apoptosis of rat granulosa cells

Reproduction ◽  
2005 ◽  
Vol 129 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Takashi Shimizu ◽  
Izumi Ohshima ◽  
Manabu Ozawa ◽  
Satoko Takahashi ◽  
Atsushi Tajima ◽  
...  
Keyword(s):  
Heat Stress ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Follicular Development ◽  
Receptor Expression ◽  
Female Rats ◽  
Aromatase Activity ◽  
Mrna Levels ◽  
Gonadotropin Receptor ◽  
Estradiol Levels

Heat stress inhibits ovarian follicular development in mammalian species. We hypothesized that heat stress inhibits the function of follicular granulosa cells and suppresses follicular development. To test this, immature female rats were injected with pregnant mare serum gonadotropin (PMSG) at 48 h after the start of temperature treatment (control: 25 °C, 50% RH; heat stress: 35 °C, 70% Relative Humidity). The ovaries and granulosa cells of follicles at different developmental stages were analyzed for gonadotropin receptor levels and aromatase activity; estradiol levels were measured in follicular fluid. Before injection, heat stress diminished only the amount of FSH receptor on granulosa cells of antral follicles. During PMSG-stimulated follicular development, heat stress strongly inhibited gonadotropin receptor levels and aromatase activity in granulosa cells, and estradiol levels in the follicular fluid of early antral, antral and preovulatory follicles. To examine apoptosis and mRNA levels of bcl-2 and bax in granulosa cells, follicles harvested 48 h after PMSG injection were cultured in serum-free conditions. Heat-stressed granulosa cells showed a time-dependent increase in apoptosis. The bcl-2 mRNA levels were similar in control and heat-stressed granulosa cells; bax mRNA levels were increased in heat-stressed granulosa cells. According to these results, heat stress inhibits expression of gonadotropin receptors in granulosa cells and attenuates estrogenic activity of growing follicles, granulosa cells of heat-stressed follicles are susceptible to apoptosis, and the bcl2/bax system is not associated with heat-stress-induced apoptosis of granulosa cells. Our study suggests that decreased numbers and function of granulosa cells may cause ovarian dysfunction in domestic animals in summer.

Altrenogest affects the development and endocrine milieu of ovarian follicles in prepubertal and mature gilts†

2020 ◽  
Vol 103 (5) ◽  
pp. 1069-1084
Author(s):  
Adam J Ziecik ◽  
Klaudia Drzewiecka ◽  
Katarzyna Gromadzka-Hliwa ◽  
Jan Klos ◽  
Patrycja Witek ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Messenger Rna ◽  
Ovarian Follicle ◽  
Adenosine Monophosphate ◽  
Cyst Formation ◽  
Ovarian Follicles ◽  
Mrna Levels ◽  
Molecular Control ◽  
Antral Follicles ◽  
Follicular Cyst

Abstract Altrenogest with gonadotropins is commonly used to synchronize the estrous cycle, but it can also lead to follicular cyst formation, especially in prepubertal gilts. Here, we aimed to investigate how maturity and altrenogest treatment affect the development, endocrine milieu, and molecular control of ovarian follicles. Crossbred prepubertal and mature gilts were challenged or not (control) with altrenogest, and ovaries were collected in the morning on the first day of behavioral estrus. In prepubertal gilts, altrenogest decreased the percentage of primordial and atretic small follicles, but increased large antral follicles when compared with controls. In mature gilts, altrenogest reduced the percentage of primary follicles and elevated the total number of antral follicles. Maturity affected the estradiol level in the follicular fluid of preovulatory follicles, luteinizing hormone (LH)-stimulated cyclic adenosine monophosphate (cAMP) generation, and LH receptor messenger RNA (mRNA) expression in granulosa. Moreover, cytochrome P45017A1 (CYP17A1) mRNA levels in the theca layer were affected and correlated with follicular androstendione and estradiol concentration. Altrenogest negatively affected follicular fluid progesterone concentration and decreased levels of prostaglandin (PG) E2 in prepubertal gilts and PGF2alpha metabolite in mature gilts. LH-stimulated cAMP release in granulosa cells of mature gilts as well as human chorionic gonadotropin- and forskolin-induced cAMP were also affected. In addition, altrenogest downregulated CYP17A1 mRNA in the prepubertal theca layer and PGF2alpha synthase expression in the granulosa and theca layer of mature gilts. To the best of our knowledge, this is the first study to report multiple effects of maturity and altrenogest on the endocrine milieu and molecular regulations governing ovarian follicle development in gilts.

Relationships between concentrations of steroids, igf-1 and igf binding proteins during follicular development in the ewe

1995 ◽  
Vol 1995 ◽  
pp. 56-56
Author(s):  
M. Khalid ◽  
W. Haresign
Keyword(s):  
Follicular Fluid ◽  
Biochemical Markers ◽  
Binding Proteins ◽  
Ovarian Function ◽  
Follicular Development ◽  
Physiological Status ◽  
Igf Binding Proteins ◽  
Ovarian Cells ◽  
Igf Binding

Insulin-like growth factor-1 (IGF-1) is one of the potential autocrine/paracrine regulators of ovarian function. Not only do relationships exist between follicular fluid concentrations of IGF-1 and various biochemical markers of follicular differentiation, but IGF-1 has also been shown to stimulate both proliferation and steroidogenesis in ovarian cells in vitro (Adashi et al., 1985). The actions of IGF-1 are thought to be modulated by IGF-binding proteins (IGFBPs). Indeed, follicular growth and atresia in the ewe have been reported to be determined more by changes in IGFBPs than by changes in IGF-1 (Monget et al., 1993). However, in mat particular study, stage of follicular development was determined by follicle size and by microscopic examination of the granulosa cells of individual follicles rather than by biochemical markers of follicle status. The objective of the present study was, therefore, to investigate changes in IGF-1 and IGFBPs levels in follicular fluid and to relate these to the physiological status as determined by steroidogenic content of follicular fluid.

Sign in / Sign up

Export Citation Format

Share Document