The Influence of Chromosomal Polymorphism on Embryo Development and Embryonic Molecular Karyotype in Preimplantation Genetic Testing for Chromosomal Translocation

Traditionally, chromosomal polymorphisms (CPMs) are normal genetic variants in individuals with no phenotypic variations. However, some studies have shown that CPM is related to reproductive diseases. We explored the influence of CPM on embryonic development and molecular karyotype in chromosomal translocation (CT) patients undergoing preimplantation genetic testing (PGT) between February 2013 and May 2019. Twenty-six cases with CPM and 56 controls with normal chromosomes were included. Furthermore, a 1:4 match pair analysis by female age included 39 cases with CTCPM and 185 controls with CT. There was no statistical difference in fertilization rate (78.48% vs. 78.33%), cleavage rate on Day 3 (90.32% vs. 89.16%), blastocyst rate (60.00% vs. 60.80%), and the high-quality blastocyst rate (36.31% vs. 35.22%) between CPM and normal chromosomes. The high-quality blastocyst rate of CTCPM was significantly lower than that for CT (26.78% vs. 38.89%). Moreover, there was no statistical difference in fertilization rate (70.65% vs. 70.37%), cleavage rate on Day 3 (88.67% vs. 89.53%), and blastocyst rate (48.48% vs. 53.17%) between CTCPM and CT. In addition, one CTCPM spouse had a lower high-quality blastocyst rate, especially of males with CTCPM. Abnormal embryo rates of CTCPM were significantly higher than those for CT (78.64% vs. 68.93%). Abnormal embryo rates were higher in both CTCPM and CPM paternal carriers with CT partners, respectively. For CT, CTCPM may have an impact on the high-quality blastocyst rate and embryonic molecular karyotype, especially in male patients. Patients with CTCPM are relatively rare, but this population would benefit from being explored using a larger sample size.

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The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype

10.21203/rs.2.14678/v4 ◽

2020 ◽

Author(s):

Gang Li ◽

Weiyi Shi ◽

Wenbin Niu ◽

Jiawei Xu ◽

Yihong Guo ◽

...

Keyword(s):

Embryonic Development ◽

High Quality ◽

Preimplantation Genetic Testing ◽

Abnormal Embryo ◽

Significant Difference ◽

Molecular Karyotype ◽

Group A ◽

The Difference ◽

Group B ◽

Euploid Embryo

Abstract Background: Balanced complex chromosome rearrangements (BCCR) are balanced chromosomal structural aberrations that involve two or more chromosomes and at least three breakpoints. It is very rare in the population.The objective is to explore the difference of influence of three types of BCCR on early embryonic development and molecular karyotype.Results:Twelve couples were recruited including four couples of three-way rearrangements carriers (group A), three couples of double two-way translocations carriers (group B) and five couples of exceptional CCR carriers (group C). A total of 243 oocytes were retrievedin the seventeen preimplantation genetic testing (PGT) cycles, and 207 of these were available for fertilization.After intracytoplasmic sperm injection, 181oocytes normally fertilized. The rates of embryos forming on day3 in three groups were 87.88%, 97.78% and77.14%, which was significantly different (P=0.01). Compared with group B, the rate of embryo formation was statistically significantly lower in group C(P=0.01).Furthermore, the rates of high-quality blastocysts in three group were 14.71%, 48.15% and 62.96%, respectively, which was significantly different (P=0.00). Compared with group B andC, the rate of high-quality blastocysts in group A was statistically significantly lower (P=0.00;P=0.00). Comprehensive chromosome analysis was performed on 83 embryos, including 75 trophectodermcellsand 8 blastomeres. Except 7 embryos failed to amplify, 9.01%embryos were diagnosed as euploidy, and 90.91% were diagnosed as abnormal. As for group A, the euploid embryo rate was 10.71%and the abnormal embryo rate was 89.29%. In group B,the euploid embryo rate was 3.85%, the abnormal embryo rate was 96.15%. The euploid embryo rate was 13.04%, the abnormal embryo rate was 86.96% in group C.There were no significant differences among the three groups (P = 0.55).Conclusions:The lowest rate of high quality blastocysts has been for three-way rearrangements and the lowest rate of euploidy has been for double two-way translocations, although no significant difference. Different types of BCCR maybe have little effect on the embryonic molecular karyotype. The difference of influence of BCCR on early embryonic developmentandmolecular karyotypeshould be further studied.

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The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype

10.21203/rs.2.14678/v5 ◽

2020 ◽

Author(s):

Gang Li ◽

Weiyi Shi ◽

Wenbin Niu ◽

Jiawei Xu ◽

Yihong Guo ◽

...

Keyword(s):

Embryonic Development ◽

High Quality ◽

Preimplantation Genetic Testing ◽

Abnormal Embryo ◽

Significant Difference ◽

Molecular Karyotype ◽

Group A ◽

The Difference ◽

Group B ◽

Euploid Embryo

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The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype

10.21203/rs.2.14678/v3 ◽

2020 ◽

Author(s):

Gang Li ◽

Weiyi Shi ◽

Wenbin Niu ◽

Jiawei Xu ◽

Yihong Guo ◽

...

Keyword(s):

Embryonic Development ◽

High Quality ◽

Preimplantation Genetic Testing ◽

Abnormal Embryo ◽

Significant Difference ◽

Molecular Karyotype ◽

Group A ◽

The Difference ◽

Group B ◽

Euploid Embryo

Abstract Background: Balanced complex chromosome rearrangements (BCCR) are balanced chromosomal structural aberrations that involve two or more chromosomes and at least three breakpoints. It is very rare in the population.The objective is to explore the difference of influence of three types of BCCR on early embryonic development and molecular karyotype.Results:Twelve couples were recruited including four couples of three-way rearrangements carriers (group A), three couples of double two-way translocations carriers (group B) and five couples of exceptional CCR carriers (group C). A total of 243 oocytes were retrievedin the seventeen preimplantation genetic testing (PGT) cycles, and 207 of these were available for fertilization.After intracytoplasmic sperm injection, 181oocytes normally fertilized. The rates of embryos forming on day3 in three groups were 87.88%, 97.78% and77.14%, which was significantly different (P=0.01). Compared with group B, the rate of embryo formation was statistically significantly lower in group C(P=0.01).Furthermore, the rates of high-quality blastocysts in three group were 14.71%, 48.15% and 62.96%, respectively, which was significantly different (P=0.00). Compared with group B andC, the rate of high-quality blastocysts in group A was statistically significantly lower (P=0.00;P=0.00).Comprehensive chromosome analysis was performed on 83 embryos, including 75 trophectodermcellsand 8 blastomeres. Except 7 embryos failed to amplify, 9.01%embryos were diagnosed as euploidy, and 90.91% were diagnosed as abnormal. As for group A, the euploid embryo rate was 10.71%and the abnormal embryo rate was 89.29%. In group B,the euploid embryo rate was 3.85%, the abnormal embryo rate was 96.15%. The euploid embryo rate was 13.04%, the abnormal embryo rate was 86.96% in group C.There were no significant differences among the three groups (P = 0.55).Conclusions:The lowest rate of high quality blastocysts has been for three-way rearrangements and the lowest rate of euploidy has been for double two-way translocations, although no significant difference. Different types of BCCR maybe have little effect on the embryonic molecular karyotype. The difference of influence of BCCR on early embryonic developmentandmolecular karyotypeshould be further studied.

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Morphokinetic parameters in chromosomal translocation carriers undergoing preimplantation genetic testing

Reproductive BioMedicine Online ◽

10.1016/j.rbmo.2018.11.006 ◽

2019 ◽

Vol 38 (2) ◽

pp. 177-183

Author(s):

Jenna Lammers ◽

Arnaud Reignier ◽

Carole Splingart ◽

Kamran Moradkhani ◽

Paul Barrière ◽

...

Keyword(s):

Genetic Testing ◽

Chromosomal Translocation ◽

Preimplantation Genetic Testing ◽

Morphokinetic Parameters

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Efficiency Comparison Between Dry And Humid Cultures For Clinical Outcome of The In Vitro Fertilization-Embryo Transfer

10.21203/rs.3.rs-335124/v2 ◽

2021 ◽

Author(s):

Weihai Xu ◽

Lin Zhang ◽

Ling Zhang ◽

Shishi Li ◽

Jing Shu

Keyword(s):

In Vitro Fertilization ◽

Embryo Transfer ◽

Implantation Rate ◽

Fertilization Rate ◽

Cleavage Rate ◽

High Quality ◽

Vitro Fertilization ◽

Efficiency Comparison ◽

Ivf Et

Abstract Background: In this study, we compared the in vitro embryo development, embryo transfer outcome and the offspring outcome in the in vitro fertilization-embryo transfer (IVF-ET) between dry culture (DC) and humid culture (HC). Methods: Our study was divided into two parts. Firstly, we determined the fertilization rate, cleavage rate and high-quality embryo rate from 21 cycles in the DC group (N=262 oocytes) and HC group (N=263 oocytes). Secondly, we determined the embryo transfer outcome and the offspring outcome in DC group (N=184 cycles) and HC group (N=136 cycles). Results: Compared with the HC group, significant increase was observed in the high-quality embryo rate (66.1.2% vs. 55.3%, p=0.037) and implantation rate (49.8% vs. 40.6%, p=0.027) in the DC group. No statistical differences were observed in the pregnant outcome and birth defect of the offspring (p>0.05). Compared with HC, DC was associated with a higher high-quality embryo rate and a higher implantation rate after embryo transfer. Conclusions: No statistical differences were noticed in the offspring conditions between the two culture modes. Taken together, DC may serve as a promising method for IVF-ET.

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The influence of balanced complex chromosomal rearrangements on preimplantation embryonic development potential and molecular karyotype

10.21203/rs.2.14678/v2 ◽

2019 ◽

Author(s):

Gang Li ◽

Weiyi Shi ◽

Wenbin Niu ◽

Jiawei Xu ◽

Yihong Guo ◽

...

Keyword(s):

Embryonic Development ◽

Formation Rate ◽

Early Embryonic Development ◽

High Quality ◽

Abnormal Embryo ◽

Molecular Karyotype ◽

Group A ◽

The Difference ◽

Group B ◽

Euploid Embryo

Abstract Background: Balanced complex chromosome rearrangements (BCCR) are balanced chromosomal structural aberrations that involve two or more chromosomes and at least three breakpoints. It is very rare in the population. The objective is to explore the difference of influence of three types of BCCR on early embryonic development and molecular karyotype. Results: Twelve couples were recruited including four couples of three-way rearrangements carriers (group A), three couples of double two-way translocations carriers (group B) and five couples of exceptional CCR carriers (group C). A total of 243 oocytes were retrieved in the seventeen preimplantation genetic testing (PGT) cycles, and 207 of these were available for fertilization. After intracytoplasmic sperm injection, 181 oocytes normally fertilized.The rates of embryos forming on day3 in three groups were 87.88%, 97.78% and 77.14%, which was significantly different (P=0.01). Compared with group B, the rate of embryo formation was statistically significantly lower in group C (P=0.01). Furthermore, the rates of high-quality blastocysts in three group were 14.71%, 48.15% and 62.96%, respectively, which was significantly different (P=0.00). Compared with group B and C, the rate of high-quality blastocysts in group A was statistically significantly lower (P=0.00; P=0.00). Comprehensive chromosome analysis was performed on 83 embryos, including 75 trophectoderm cells and 8 blastomeres. Except 7 embryos failed to amplify, 9.01% embryos were diagnosed as euploidy, and 90.91% were diagnosed as abnormal. As for group A, the euploid embryo rate was 10.71% and the abnormal embryo rate was 89.29%. In group B, the euploid embryo rate was 3.85%, the abnormal embryo rate was 96.15%. The euploid embryo rate was 13.04%, the abnormal embryo rate was 86.96% in group C. There were no significant differences among the three groups (P = 0.55). Conclusions: The double two-way translocations couples have more chance to get balanced or normal embryos probably, and there may be more high-quality blastocysts in exceptional CCRs, but the blastocyst formation rate was similar among the three type of BCCR. Different types of BCCR maybe have little effect on the embryonic molecular karyotype. The difference of influence of BCCR on early embryonic development and molecular karyotype should be further studied.

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Effects of Different Types of Incubators on Embryo Development and Clinical Outcomes

10.5772/intechopen.97833 ◽

2021 ◽

Author(s):

Ji Liu ◽

Yan-Hua Zhou ◽

Xiao-Xiao Wang ◽

Ling-Xi Tong ◽

Yan-Hong Li ◽

...

Keyword(s):

Clinical Outcomes ◽

Embryo Development ◽

Recovery Time ◽

Culture Media ◽

Fertilization Rate ◽

High Quality ◽

Water Jacket ◽

Different Types ◽

Temperature Recovery ◽

Blastocyst Rate

Main differences of incubators are humidity, temperature and gas control ways, which play important roles in regulating the steady state of culture media. In this study, we compared the effects of different types of incubators (air jacket incubators and water jacket incubators) on embryo development and clinical outcomes in human assisted reproduction. We found that temperature recovery time in air jacket incubators was significantly shorter than that in water jacket incubators. Although the O2 recovering time was also significantly shorter in air jacket incubators as compared with the water jacket incubator, no significant differences were observed in CO2 recovering time between two groups, which was also verified by pH recovering time of culture media. Besides, the temperature of culture medium in the dish covered with oil recovered more quickly in the air jacket incubators. However, there were no significant differences observed in the fertilization rate, Day 3 high-quality embryo rate, blastocyst rate, good blastocyst rate and clinical outcomes between two groups. These results indicate that the microenvironment, especially the temperature, in air jacket incubator recover faster than that in water jacket incubators, however, there were no significant differences in embryo development and clinical outcomes between two types of incubators.

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MicroRNA Expression Profiles Analysis in Sperm Reveals hsa-mir-191 is an Auspicious Omen of in Vitro Fertilization

10.21203/rs.2.12439/v1 ◽

2019 ◽

Author(s):

Hua Xu ◽

Xin Wang ◽

Zhikai Wang ◽

Jianhui Li ◽

Zhiming Xu ◽

...

Keyword(s):

Embryonic Development ◽

Small Rna ◽

Expression Profiles ◽

Fertilization Rate ◽

Small Rna Sequencing ◽

High Quality ◽

Potential Marker ◽

Blastocyst Rate ◽

The Relationship ◽

Early Human

Abstract Background: MicroRNAs(miRNAs) are a class of non-coding small RNAs, which play important roles in many physiological processes by regulating gene expression. Previously studies show that the expression level of total miRNAs increased during the progression of mouse embryonic development, and some miRNAs control the regulatory network in the development progression. However, there are few studies focused on effects of miRNA on the early human embryonic development. The relationship of between miRNAs and early human embryogenesis is still unknown. Results In this study, 102 male sperm samples that were treated with assisted reproductive technology but with normal sperm index were collected for small RNA sequencing, and the relationships between differentially expressed small RNA and fertilization rate, blastocyst rate and high-quality embryo rate were analyzed. The results showed that the sperm samples with high expression of hsa-mir-191 had higher fertilization rate, blastocyst rate and high-quality embryo rate. Using hsa-mir-191 as a single indicator to predict high-quality embryo rate, the ROC curve has an AUC of 0.686. At the same time, we also found that the expression of hsa-mir-191 has a certain correlation with sperm abnormality rate (cor = 0.29, p< 0.01). In addition, we also evaluated the relationship between hsa-mir-34c and human early embryo development in these 102 sperm samples, and obtained negative results. Conclusions These findings suggest that higher hsa-mir-191-5p expression with better human early embryonic development, and indicate that the hsa-mir-191-5p could be a potential marker to access the quality of IVF.

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Preimplantation genetic testing for balanced complex chromosomal rearrangement carriers by comprehensive chromosome screening

10.21203/rs.2.9882/v1 ◽

2019 ◽

Author(s):

Gang Li ◽

Weiyi Shi ◽

Wenbin Niu ◽

Jiawei Xu ◽

Yihong Guo ◽

...

Keyword(s):

Genetic Testing ◽

Snp Array ◽

Chromosome Analysis ◽

High Rate ◽

Normal Embryo ◽

Ovum Donation ◽

Preimplantation Genetic Testing ◽

Abnormal Embryo ◽

Comprehensive Chromosome Screening ◽

Structural Aberrations

Abstract Background: Balanced complex rearrangements (BCCRs) are balanced chromosomal structural aberrations that involve two or more chromosomes and at least three breakpoints. It is very rare in the population. Whether the couple of BCCRs benefit from preimplantation genetic testing (PGT) need to be further explored. Here, we reported the outcome of PGT in BCCRs carriers. Results: A total of 141 oocytes were retrieved from 7 couples within 10 PGT cycles, including 116 mature oocytes (MII), and 94 (81.03%) oocytes normally fertilized after intracytoplasmic sperm injection (ICSI). Then, 47 embryos were biopsied, including 8 embryos at the cleavage stage and 39 (41.49%) blastocysts. After comprehensive chromosome analysis, the balanced or normal embryo rate was 11.36% (5), the abnormal embryo rate was 88.63% (39), and 3 failed to amplify. Among them, the balanced or normal embryo rate was 33.33% (3) and the abnormal embryo rate was 66.67% (6) in the three-way rearrangements. The balanced or normal embryo rate was 5.6% (1) and the abnormal embryo rate was 94.4% (17) in double two-way translocations. The balanced or normal embryo rate was 5.9% (1) in exceptional CCRs, and the abnormal embryo rate was 94.1% (16). There were no significant differences among the three groups (P=0.11). In the 10 PGT cycles, there were 7 cycles in which no embryo could be transplanted and 3 cycles in which balanced or normal embryos underwent frozen-thawed embryo transplantation. One of the 3 cycles was clinically pregnant, and the prenatal diagnosis of amniocytes using G-band and SNP array at 16 weeks of gestation was 46, XN, and a boy was born alive and healthy. Conclusions: BCCR carriers have a high rate of obtaining abnormal embryos, but they can also have healthy offspring. For BCCR carriers with fertility needs, PGT is recommended to have related offspring, or they can choose sperm donor or ovum donation-assisted reproduction.

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Effects of Gender of Reciprocal Chromosomal Translocation on Blastocyst Formation and Pregnancy Outcome in Preimplantation Genetic Testing

Frontiers in Endocrinology ◽

10.3389/fendo.2021.704299 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hui Song ◽

Hao Shi ◽

En-tong Yang ◽

Zhi-qin Bu ◽

Zi-qi Jin ◽

...

Keyword(s):

Genetic Testing ◽

Pregnancy Outcome ◽

Reciprocal Translocation ◽

Maternal Age ◽

Formation Rate ◽

Chromosomal Translocation ◽

Blastocyst Formation ◽

Preimplantation Genetic Testing ◽

Group A ◽

Group B

ObjectiveTo determine the effect of gender of reciprocal chromosomal translocation on blastocyst formation and pregnancy outcome in preimplantation genetic testing, including different parental ages.MethodsThis was a retrospective cohort study that enrolled 1034 couples undergoing preimplantation genetic testing-structural rearrangement on account of a carrier of reciprocal chromosomal translocation from the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from January 2015 to December 2019. Group A represented 528 couples in which the man was the carrier of reciprocal translocation and group B represented 506 couples in which the woman was the carrier of reciprocal translocation. All patients were divided into two groups according to their age: female age<35 and female age≥35. Furthermore, the differences in blastocyst condition and pregnancy outcome between male and female carriers in each group were further explored according to their father’s age.ResultsThe blastocyst formation rate of group A (55.3%) is higher than that of group B (50%) and the results were statistically significant (P<0.05). The blastocyst formation rate of group A is higher than that of group B, no matter in young maternal age or in advanced maternal age (P<0.05). The blastocyst formation rate in maternal age<35y and paternal age<30y in group A(57.1%) is higher than that of Group B(50%); Similarly, the blastocyst formation rate in maternal age≥35 and paternal age≥38y(66.7%) is higher than that of Group B(33.3%)(all P<0.05). There was no difference in fertilization rate, aeuploidy rate, clinical pregnancy rate, miscarriage rate and live birth rate between Group A and Group B.ConclusionWhen the carrier of reciprocal translocation is male, the blastocyst formation rate is higher than that of female carrier. While there is no significant difference between the two in terms of fertilization rate, aeuploidy rate, clinical pregnancy rate, miscarriage rate and live birth rate.

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