scholarly journals RNAi-Mediated Silencing of Pgants Shows Core 1 O-Glycans Are Required for Pupation in Tribolium castaneum

2021 ◽  
Vol 12 ◽  
Author(s):  
Weidong Li ◽  
Kristof De Schutter ◽  
Els J. M. Van Damme ◽  
Guy Smagghe

Protein glycosylation is one of the most common and most important post-translational modifications. Despite the growing knowledge on N-glycosylation, the research on O-glycosylation is lagging behind. This study investigates the importance of O-glycosylation in the post-embryonic development of insects using the red flour beetle, Tribolium castaneum, as a model. We identified 28 O-glycosylation-related genes (OGRGs) in the genome of the red flour beetle. 14 OGRGs were selected for functional analysis based on their involvement in the initial attachment of the carbohydrate in the different O-glycosylation pathways or the further elongation of the most abundant O-glycans and, in addition, showing severe RNAi-induced phenotypes in Drosophila melanogaster. The expression profile of these OGRGs was mapped throughout the developmental stages of the insect and in the different tissues of the pupa and adult. Subsequently, these genes were silenced using RNA interference (RNAi) to analyze their role in development. A broad spectrum of phenotypes was observed: from subtle effects and disrupted wing formation when silencing the genes involved in O-mannosylation, to blockage of pupation and high mortality after silencing of the genes involved in O-GalNAc and core 1 O-glycan (O-GalNAc-Gal) synthesis. RNAi experiments were also performed to assess the effects of blocking multiple pathways of O-glycosylation. However, the observed phenotypes induced by multiple RNAi were similar to those of the single gene RNAi experiments. The silencing of OGRGs often resulted in high mortality and wing phenotypes, indicating the importance of O-glycosylation for the survival of the insect and the formation of wings during the post-embryonic development of T. castaneum.

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e65125 ◽  
Author(s):  
Amanda Fraga ◽  
Lupis Ribeiro ◽  
Mariana Lobato ◽  
Vitória Santos ◽  
José Roberto Silva ◽  
...  

2014 ◽  
Vol 50 (1) ◽  
pp. 17-26 ◽  
Author(s):  
Chieka Minakuchi ◽  
Takahiro Ohde ◽  
Ken Miura ◽  
Toshiharu Tanaka ◽  
Teruyuki Niimi

2020 ◽  
Vol 121 ◽  
pp. 104017 ◽  
Author(s):  
Shouya Naruse ◽  
Yumiko Washidu ◽  
Ken Miura ◽  
Tetsuro Shinoda ◽  
Chieka Minakuchi

Author(s):  
N. Ahmed ◽  
A. Ali ◽  
S. A. Memon ◽  
T. K. Qambrani ◽  
G. Khaliq

Red flour beetle (Tribolium castaneum) is the major insect pest of store grain, and its single larvae can attack 88 grains during its life which leads to a considerable loss of quality and viability of grain. Therefore, to manage this insect pest present research was designed to check the effects of different colours of lights on the developmental stages of red flour beetle and their attraction on lights. Six (White, Yellow, Red, Green, Blue and Black) colours of light were used in the present experiment at 30±2oC and 60%±10% relative humidity (RH). Results revealed that a longer incubation period (4.17±0.18, less hatching (78.66±0.50%) and highest mortality (21.34±1.11%) of T. castaneum eggs were observed on blue light, while a short incubation period (3.03±0.51), highest hatching (89.99±1.92%) and lowest mortality (10.01±0.22%) were observed on white colour of light. However, a longer duration (32.01±0.15, 12.33±0.31) and maximum (28.10±2.50 ,14.66±0.52) mortality of T. castaneum was found in larval and pupae stages on blue colour of light, while a short duration (21.27±0.27, 7.33±0.13) and minimum (8.30±2.5, 8.00±0.65) larval and pupal  mortality (%) were seen by red and yellow colour of lights. Similarly, an adult’s longevity significantly reduced (48.33±1.76) when beetles were exposed on blue light, whereas a significant (62.33±1.66) increase in adult longevity was found on white colour of light. A highest (33.33±0.56) attraction of red flour beetle was found on red colour of light, whereas, a lowest attraction was observed on blue (6.00±0.85) and black (10.00±1.55) colours of lights.


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