scholarly journals Neuropeptide Bursicon Influences Reproductive Physiology in Tribolium Castaneum

2021 ◽  
Vol 12 ◽  
Author(s):  
Jingjing Li ◽  
Zidan Zhu ◽  
Jingxiu Bi ◽  
Qili Feng ◽  
Brenda T. Beerntsen ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Penaeus Monodon ◽  
Adult Emergence ◽  
Underlying Mechanism ◽  
Reproductive Physiology ◽  
Tor Signaling ◽  
Larval Size ◽  
Ribosomal Protein S6 ◽  
Cuticle Sclerotization ◽  
Genes Encoding

Bursicon is a neuropeptide belonging to the cystine knot family and is composed of burs and partner of burs (pburs) subunits. It can form heterodimers or homodimers to execute different biological functions. Bursicon heterodimers regulate cuticle sclerotization and wing maturation, whereas bursicon homodimers mediate innate immunity and midgut stem cell proliferation. A recent study has shown that bursicon potentially induces the expression of vitellogenin (Vg) in the black tiger shrimp Penaeus monodon; however, the underlying mechanism remains unknown. In this study, we investigated the role of bursicon in the reproductive physiology of the red flour beetle, Tribolium castaneum. The knockdown of burs, pburs, or its receptor T. castaneum rickets (Tcrk) in 2-day pupae significantly downregulated the expression levels of Vg1, Vg2, and Vg receptor (VgR) genes in females 3- and 5-day post-adult emergence, leading to abnormal oocytes with limited Vg content. The silencing of burs repressed the number of eggs laid and completely inhibited egg hatch, whereas the silencing of pburs dramatically decreased the number of eggs laid, hatch rate, and offspring larval size, and this RNA interference (RNAi) effects persisted to the next generation. Furthermore, the knockdown of burs or pburs downregulated the expression of the insulin/insulin-like signaling/target of rapamycin (TOR) signaling genes encoding insulin receptor (InR), protein kinase B (Akt), TOR, and ribosomal protein S6 kinase (S6K). Most importantly, the injection of recombinant pburs (r-pburs) protein was able to upregulate the expression of Vg, VgR, InR, Akt, TOR, S6K, JH synthesis (JHAMT), Methoprene-tolerant (Met), and Taiman (Tai) in normal females and rescue the expression of Vg and VgR in pburs RNAi females but failed to rescue Vg and VgR in Tcrk knockdown females. We infer that bursicon homodimers influence Vg expression via the receptor Tcrk, possibly by mediating the expression of the juvenile hormone (JH) and IIS/TOR pathway genes, thereby regulating reproduction in T. castaneum.

scholarly journals The 40S Ribosomal Protein S6 Response to Blue Light by Interaction with SjAUREO in Saccharina japonica

2019 ◽  
Vol 20 (10) ◽  
pp. 2414 ◽  
Author(s):  
Hexiang Luan ◽  
Jianting Yao ◽  
Zhihang Chen ◽  
Delin Duan
Keyword(s):  
Ribosomal Protein ◽  
Cdna Library ◽  
Blue Light ◽  
Cell Cycle Progression ◽  
Saccharina Japonica ◽  
Cdna Libraries ◽  
Cellular Division ◽  
Cycle Progression ◽  
Ribosomal Protein S6 ◽  
Genes Encoding

Blue light (BL) plays an important role in regulation of the growth and development of aquatic plants and land plants. Aureochrome (AUREO), the recent BL photoreceptor identified in photosynthetic stramenopile algae, is involved in the photomorphogenesis and early development of Saccharina japonica porophytes (kelp). However the factors that interact with the SjAUREO under BL conditions specifically are not clear. Here in our study, three high quality cDNA libraries with CFU over 5 × 106 and a recombination rate of 100% were constructed respectively through white light (WL), BL and darkness (DK) treatments to the juvenile sporophytes. Based on the constructed cDNA libraries, the interactors of SjAUREO were screened and analyzed. There are eighty-four genes encoding the sixteen predicted proteins from the BL cDNA library, sixty-eight genes encoding eighteen predicted proteins from the DK cDNA library, and seventy-four genes encoding nineteen proteins from the WL cDNA library. All the predicted proteins are presumed to interact with SjAUREO when co-expressed with SjAUREO seperately. The 40S ribosomal protein S6 (RPS6), which only exists in the BL treated cDNA library except for two other libraries, and which is essential for cell proliferation and is involved in cell cycle progression, was selected for detailed analysis. We showed that its transcription was up-regulated by BL, and was highly transcribed in the basal blade (meristem region) of juvenile sporophytes but less in the distal part. Taken together, our results indicated that RPS6 was highly involved in BL-mediated kelp cellular division and photomorphogenesis by interacting with SjAUREO.

scholarly journals The effect of different selection intensities on selection response in egg-laying of Tribolium castaneum

1975 ◽  
Vol 25 (1) ◽  
pp. 17-27 ◽  
Author(s):  
R. G. Ruano ◽  
F. Orozco ◽  
C. López-Fanjul
Keyword(s):  
Tribolium Castaneum ◽  
Adult Emergence ◽  
Selection Response ◽  
Fixed Number ◽  
Egg Laying ◽  
Short Term ◽  
Selection Intensities ◽  
Number Of Individuals ◽  
Rate Of Response ◽  
Good Agreement

SUMMARYAn experiment was carried out to test the effect of varying selection intensity on the response to individual selection with a fixed number of individuals scored per generation. The selected trait was egg laying of virgin females of Tribolium castaneum scored from the 7th to the 1 lth day after adult emergence. Five different selected proportions of females were considered (5, 10, 20, 33 and 50%) and each treatment was represented by two replicate lines. Control lines were maintained throughout the experiment. The lines selected at the lowest proportions (5 and 10%) led to the largest initial gains, but the largest final gains were achieved, by the lines where the proportions selected were 10 and 20%, in agreement with theory. Lines where the proportion selected was 50% gave the lowest rate of response over the period considered (32 generations). A good agreement was found between predicted and realized short-term responses to selection. Prediction at later stages of selection deteriorated in the most strongly selected lines mainly due to the levels of inbreeding attained.

scholarly journals Effect of coffee on the growth and development of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae

2015 ◽  
Vol 42 (2) ◽  
pp. 211-216 ◽  
Author(s):  
Md Mostakim ◽  
Ataur Rahman Khan
Keyword(s):  
Tribolium Castaneum ◽  
Growth And Development ◽  
Coffea Arabica ◽  
Adult Emergence ◽  
Red Flour Beetle ◽  
Flour Beetle ◽  
Botanical Pesticide ◽  
Coleoptera Tenebrionidae

Effect of various concentrations of coffee, Coffea arabica, viz. 12.50%, 25% and 50% on the growth and development of the red flour beetle, Tribolium castaneum (Herbst) was determined. Coffee lengthened the larval and pupal periods significantly. There was also a significant reduction in the pupal recovery and adult emergence of the beetle following treatment. The food with 50% coffee had the most detrimental effects on the beetle. Coffee extract could be used as useful botanical pesticide for controlling red flour beetle.Bangladesh J. Zool. 42(2): 211-216, 2014

scholarly journals Toxicity Of Fenvalerate On Larvae Of The Susceptible And Resistant Strains Of Tribolium Castaneum (Herbst)

1970 ◽  
Vol 6 ◽  
pp. 27-31
Author(s):  
Saiful Islam Faruki ◽  
Rogena Yeasmin ◽  
Dipali Rani Das
Keyword(s):  
Tribolium Castaneum ◽  
Larval Mortality ◽  
Adult Emergence ◽  
Synthetic Pyrethroid ◽  
Red Flour Beetle ◽  
Flour Beetle ◽  
Post Exposure ◽  
Laboratory Conditions ◽  
Resistant Strains

Five-day old larvae of the susceptible (FSS-II) and organophosphorus-resistant (PH-I) strains of the red flour beetle Tribolium castaneum were exposed under laboratory conditions to food treated with 100, 250, 500 and 1000 ppm of a synthetic pyrethroid Fenvalerate up to adult emergence. The larval mortality was assessed at 1-, 3-, 7- and 15-days post-exposure, and up to pupation. PH-I was found less susceptible to Fenvalerate than FSS-II which required 1.2 to 3.1 fold more insecticide to induce 50% mortality. The pyrethroid treatments significantly increased the larval and pupal periods (P<0⋅05) in both the strains and the formation of pupal and adult progenies was adversely affected (P<0⋅001). DOI: http://dx.doi.org/10.3329/jles.v6i0.9717 JLES 2011 6: 27-31

COMPONENTS OF GROWTH IN GENETICALLY DIVERSE POPULATIONS OF TRIBOLIUM CASTANEUM

1969 ◽  
Vol 11 (4) ◽  
pp. 896-907 ◽  
Author(s):  
DuWayne C. Englert ◽  
A. E. Bell
Keyword(s):  
Tribolium Castaneum ◽  
Adult Emergence ◽  
Pupal Weight ◽  
Diverse Populations ◽  
Correlated Responses ◽  
Larval Weight ◽  
Developmental Patterns ◽  
Adult Weight ◽  
Total Growth ◽  
Selection For

The growth and developmental patterns of 19 genetically diverse populations of Tribolium castaneum were examined. Measurements were obtained on three primary traits (13-day larval weight, pupation time and pupal weight) and three secondary traits (number of larval molts, adult emergence time and adult weight). The populations were chosen on the basis of their previous selection histories. Selection for different primary traits produced profound effects upon the total growth and development complex.Genetic and environmental relationships between primary traits were estimated within each population, and a consistent asymmetry of correlated responses was observed to be determined by the direction of selection.Possible effects upon the disruption of an intricate balance of the three metamorphosis hormones (activation, molting and juvenile) through selection were postulated.

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